Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,759,155 G→A 5.1% L176L (TTG→TTA pksG → polyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,759,1550GA 5.1% 588.7 / 12.8 178L176L (TTG→TTApksGpolyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG
Reads supporting (aligned to +/- strand):  ref base G (91/77);  new base A (2/7);  total (93/85)
Fisher's exact test for biased strand distribution p-value = 8.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 178 total aligned reads displayed.

TCTCCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGA  >  NZ_CP034943/1759024‑1759292
                                                                                                                                   |                                                                                                                                         
tctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGa                                                                                                                                   <  1:950275/140‑1 (MQ=255)
tctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGa                                                                                                                                   <  2:1231583/140‑1 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCCGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                  >  1:534104/1‑140 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTa                                                                                                                                        <  2:1452127/134‑1 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTa                                                                                                                                        <  2:1613262/134‑1 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTa                                                                                                                                        >  1:1613262/1‑134 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                  >  1:837872/1‑140 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                  >  2:1152611/1‑140 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                  >  1:1867559/1‑140 (MQ=255)
          ccAAAGCACGGGGTCTCGCCAGTGATATTTCGCGTTTTTTAAGCGCGGACGGCGGAGACGCTTTAAGTGAAGACGGGTCTTCTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                         <  2:1945407/140‑1 (MQ=255)
                   tGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCaa                                                                                                                >  2:2338773/1‑140 (MQ=255)
                   tGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCaa                                                                                                                >  2:1904959/1‑140 (MQ=255)
                    ggTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCaaa                                                                                                               >  2:1166244/1‑140 (MQ=255)
                     gTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAAt                                                                                                              >  1:1730847/1‑140 (MQ=255)
                     gTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATACCGAGGTGTTTCAAAt                                                                                                              <  1:1152611/140‑1 (MQ=255)
                          cGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATc                                                                                                         >  2:43231/1‑140 (MQ=255)
                          cGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATc                                                                                                         >  2:779044/1‑140 (MQ=255)
                            ccAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTATTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCa                                                                                                       <  2:2368494/140‑1 (MQ=255)
                               gTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGa                                                                                                    >  1:2327373/1‑140 (MQ=255)
                               gTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGa                                                                                                    >  2:2020499/1‑140 (MQ=255)
                               gTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGa                                                                                                    >  2:1177921/1‑140 (MQ=255)
                                   tatTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCaa                                                                                                <  2:534104/140‑1 (MQ=255)
                                   tatTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCaa                                                                                                <  1:212130/140‑1 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGACTACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGTGGTGTTTCATATAGATCCAGGAGCACATGGCTa                                                                                         >  2:1081207/1‑140 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                         >  2:498489/1‑140 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                         >  1:1280985/1‑140 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                         >  2:741942/1‑140 (MQ=255)
                                           gTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTAt                                                                                        <  2:961369/140‑1 (MQ=255)
                                             tttttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTa                                                                                      <  1:968418/140‑1 (MQ=255)
                                             tttttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTa                                                                                      <  2:2305072/140‑1 (MQ=255)
                                                  aaTCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCt                                                                                 <  1:689323/140‑1 (MQ=255)
                                                       cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCGGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTGTCAAATAGATCCAGGAGCAAAGGGCTATTACGGCTATGaa                                                                            >  2:1702990/1‑140 (MQ=255)
                                                       cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGaa                                                                            >  2:859219/1‑140 (MQ=255)
                                                       cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGaa                                                                            >  2:669224/1‑140 (MQ=255)
                                                       cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAAGGGCTATTACGGCTATGaa                                                                            >  2:858733/1‑140 (MQ=255)
                                                                     gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                              <  1:1904959/140‑1 (MQ=255)
                                                                     gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                              <  2:2244693/140‑1 (MQ=255)
                                                                     gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                              <  1:2338773/140‑1 (MQ=255)
                                                                      cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                             >  1:407935/1‑140 (MQ=255)
                                                                      cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                             >  1:1421203/1‑140 (MQ=255)
                                                                          aaGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTc                                                         <  1:782524/140‑1 (MQ=255)
                                                                          aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAACTGGTTATTACGGCTATGACGTGATGGACACGTGCCgcc                                                         <  1:2188875/140‑3 (MQ=255)
                                                                          aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCgcc                                                         <  1:718760/140‑3 (MQ=255)
                                                                          aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCgcc                                                         <  1:894228/140‑3 (MQ=255)
                                                                          aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCgcc                                                         <  1:904877/140‑3 (MQ=255)
                                                                               aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                    >  2:1785391/1‑140 (MQ=255)
                                                                               aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                    >  2:1425047/1‑140 (MQ=255)
                                                                                  aCTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTccc                                                 <  1:631507/140‑1 (MQ=255)
                                                                                     ggTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGAc                                              >  1:277028/1‑140 (MQ=255)
                                                                                      gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCa                                                                                                 >  1:2403747/1‑88 (MQ=255)
                                                                                      gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCa                                                                                                 <  2:2403747/88‑1 (MQ=255)
                                                                                      gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACgg                                                                                   >  2:2029848/1‑102 (MQ=255)
                                                                                      gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACa                                             >  1:315041/1‑140 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                         <  1:1223475/59‑1 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                         >  2:1223475/1‑59 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGTATACCTGCCGTCCCATTCCCGACAGTGaa                                        >  1:1363237/1‑140 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGaa                                        >  1:1802270/1‑140 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGaa                                        >  2:1335020/1‑140 (MQ=255)
                                                                                            tGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAg                                       >  1:2229855/1‑140 (MQ=255)
                                                                                                gAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCggg                                   <  2:1318473/140‑1 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCACATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                  >  1:553788/1‑140 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCggg                                   <  2:1915302/139‑1 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                  >  2:814424/1‑140 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                  >  2:248802/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                             >  1:2105540/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                             >  1:2031158/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                             >  1:1217698/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                             >  1:1400725/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGCGAACATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGGTATGAAGTGATGGGTACCTGCCGGACCATTCCCGACCGTGAAGCTGGAGAtcg                             >  1:1358863/1‑138 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGTTGGGTGTTGTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGTCTATGAAGTGATGGATACCTGCCGTCCCATTCCTGACAGTGAAGCGGGATATTCTg                           >  1:1585762/1‑140 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                             >  1:45635/1‑74 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                             >  1:45648/1‑74 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                             >  1:1369979/1‑74 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                             >  1:1886432/1‑74 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                             <  2:45648/74‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                         <  2:879119/140‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                         <  2:2088608/140‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                         <  2:1048916/140‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                         <  1:1957698/140‑1 (MQ=255)
                                                                                                           cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                        <  2:931891/140‑1 (MQ=255)
                                                                                                           cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                        <  2:2327373/140‑1 (MQ=255)
                                                                                                           cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                        <  1:200790/140‑1 (MQ=255)
                                                                                                           cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                        <  2:508076/140‑1 (MQ=255)
                                                                                                            ggagcgggagcgGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCt                       <  1:1785391/140‑1 (MQ=255)
                                                                                                                  ggagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCttt                 <  2:1867559/140‑1 (MQ=255)
                                                                                                                   gagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTa                <  1:1166244/140‑1 (MQ=255)
                                                                                                                   gagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTa                <  2:1681527/140‑1 (MQ=255)
                                                                                                                   gagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTa                <  2:1462905/140‑1 (MQ=255)
                                                                                                                       ggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGt            <  1:1328801/140‑1 (MQ=255)
                                                                                                                        gttgatGTTTTGGTAGGCGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc           <  2:1529480/135‑1 (MQ=255)
                                                                                                                        gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                        <  2:2161239/127‑1 (MQ=255)
                                                                                                                        gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                        >  1:2161239/1‑127 (MQ=255)
                                                                                                                        gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc           >  2:611080/1‑140 (MQ=255)
                                                                                                                        gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc           <  2:841709/140‑1 (MQ=255)
                                                                                                                         tGGCTGTTTTTGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCa          <  2:375679/140‑1 (MQ=255)
                                                                                                                          ggCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCGCCCCATTCCCGACAGTGAAGCGGGAGATTCTGATCTGTCTTTAATGTCat         <  1:2020499/140‑1 (MQ=255)
                                                                                                                             tGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATc      >  1:1255080/1‑140 (MQ=255)
                                                                                                                             tGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATc      >  1:1499289/1‑140 (MQ=255)
                                                                                                                                 ttGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGa  >  2:2068808/1‑140 (MQ=255)
                                                                                                                                 ttGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGa  >  2:1651519/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                         
TCTCCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGA  >  NZ_CP034943/1759024‑1759292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: