Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,759,161 G→C 5.7% G178G (GGG→GGC pksG → polyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,759,1610GC 5.7% 603.0 / 18.0 182G178G (GGG→GGCpksGpolyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG
Reads supporting (aligned to +/- strand):  ref base G (90/81);  new base C (3/8);  total (93/89)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

TCTCCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCG  >  NZ_CP034943/1759024‑1759300
                                                                                                                                         |                                                                                                                                           
 ctcCGGGTGCCAAAGCACTGGTTATCGCCCGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                          >  1:534104/1‑140 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                          >  1:446478/1‑140 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                          >  1:1771155/1‑140 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGaa                                                                                                                                          >  1:1297697/1‑140 (MQ=255)
 ctcCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTGTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGGTGTGTTGGTAGGGGaa                                                                                                                                          >  2:2029773/1‑140 (MQ=255)
          ccAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 <  1:476920/140‑1 (MQ=255)
          ccAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 <  1:1412687/140‑1 (MQ=255)
          ccAAAGCACTGGTTATCGCCAGTGATAGTTGGCGTTTGTTAATCGCGGAGGGCGGAGCCGCTTTAAGCGACGACTGGTATTATGCCGAACCGCGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 <  2:1808398/140‑1 (MQ=255)
          ccAAAGCACGGGGTCTCGCCAGTGATATTTCGCGTTTTTTAAGCGCGGACGGCGGAGACGCTTTAAGTGAAGACGGGTCTTCTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 <  2:1945407/140‑1 (MQ=255)
                   tGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCaa                                                                                                                        >  2:2338773/1‑140 (MQ=255)
                     gTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATACCGAGGTGTTTCAAAt                                                                                                                      <  1:1152611/140‑1 (MQ=255)
                          cGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATc                                                                                                                 >  2:43231/1‑140 (MQ=255)
                          cGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATc                                                                                                                 >  2:779044/1‑140 (MQ=255)
                            ccAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGGTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCa                                                                                                               >  2:16881/1‑140 (MQ=255)
                            ccAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTATTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCa                                                                                                               <  2:2368494/140‑1 (MQ=255)
                               gTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGa                                                                                                            >  1:2327373/1‑140 (MQ=255)
                               gTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGa                                                                                                            >  2:1177921/1‑140 (MQ=255)
                                   tatTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCaa                                                                                                        <  2:534104/140‑1 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGACTACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGTGGTGTTTCATATAGATCCAGGAGCACATGGCTa                                                                                                 >  2:1081207/1‑140 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                                 >  1:835048/1‑140 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                                 >  2:741942/1‑140 (MQ=255)
                                          cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                                 >  1:318556/1‑140 (MQ=255)
                                           gTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTAt                                                                                                <  2:1170139/140‑1 (MQ=255)
                                           gTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTAt                                                                                                <  2:961369/140‑1 (MQ=255)
                                             tttttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTa                                                                                              <  1:968418/140‑1 (MQ=255)
                                             tttttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCAGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTa                                                                                              <  1:739638/140‑1 (MQ=255)
                                                     cGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATg                                                                                      <  1:2331838/140‑1 (MQ=255)
                                                       cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGaa                                                                                    >  2:859219/1‑140 (MQ=255)
                                                        tGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAg                                                                                   <  2:1730847/140‑1 (MQ=255)
                                                           gggCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGa                                                                                <  2:458541/140‑1 (MQ=255)
                                                                     gCTTTAAGTGAAGACTGGTGTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                                      <  2:2245168/140‑1 (MQ=255)
                                                                     gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                                      <  1:2338773/140‑1 (MQ=255)
                                                                     gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                                      <  1:2154439/140‑1 (MQ=255)
                                                                     gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                                      <  2:2244693/140‑1 (MQ=255)
                                                                      cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                                     >  1:1616826/1‑140 (MQ=255)
                                                                      cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                                     >  1:702079/1‑140 (MQ=255)
                                                                          aaccgAAGATTGGTCTCATGCGGACCCGAGCGCCGGAGCGCGAGCGGTGGCCGATGTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCgcc                                                                 <  1:1902706/136‑3 (MQ=255)
                                                                          aaGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTTTTACGGCTATGAAGTGATGGATACCTGCCGTc                                                                 <  1:1834905/140‑1 (MQ=255)
                                                                          aaGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTc                                                                 <  1:294482/140‑1 (MQ=255)
                                                                          aaGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTc                                                                 <  1:271509/140‑1 (MQ=255)
                                                                          aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAACTGGTTATTACGGCTATGACGTGATGGACACGTGCCgcc                                                                 <  1:2188875/140‑3 (MQ=255)
                                                                          aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCgcc                                                                 <  1:904877/140‑3 (MQ=255)
                                                                               aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                            >  2:200790/1‑140 (MQ=255)
                                                                               aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                            >  2:1425047/1‑140 (MQ=255)
                                                                                  aCTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTccc                                                         <  1:631507/140‑1 (MQ=255)
                                                                                  aCTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTccc                                                         <  1:951481/140‑1 (MQ=255)
                                                                                      gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCa                                                                                                         <  2:2403747/88‑1 (MQ=255)
                                                                                      gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCACATGGCTATTACGGCTCTGAAGTGATGGATCCCTGCCGTCCCATTCCCGaga                                                     >  2:1435082/1‑138 (MQ=255)
                                                                                      gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACa                                                     >  2:27566/1‑140 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 >  2:1223475/1‑59 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 <  1:1223475/59‑1 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGTATACCTGCCGTCCCATTCCCGACAGTGaa                                                >  1:1363237/1‑140 (MQ=255)
                                                                                           aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGaa                                                >  2:1335020/1‑140 (MQ=255)
                                                                                            tGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAg                                               >  1:2229855/1‑140 (MQ=255)
                                                                                                gAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCggg                                           <  2:1318473/140‑1 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGACAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                          >  1:1574780/1‑140 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCACATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                          >  1:553788/1‑140 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCggg                                           <  2:1915302/139‑1 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCggg                                           >  1:1915302/1‑139 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                          >  2:814424/1‑140 (MQ=255)
                                                                                                 aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                          >  2:666458/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 >  1:85538/1‑48 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAg                                                                                                                                 <  2:85538/48‑1 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                     >  1:2031158/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                     >  1:2105540/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                     >  1:1217698/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                     >  1:1788335/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                     >  1:2111258/1‑140 (MQ=255)
                                                                                                      aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGCGAACATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGGTATGAAGTGATGGGTACCTGCCGGACCATTCCCGACCGTGAAGCTGGAGAtcg                                     >  1:1358863/1‑138 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                     >  1:1369979/1‑74 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                     <  2:45635/74‑1 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                     >  1:45648/1‑74 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                     <  2:1886432/74‑1 (MQ=255)
                                                                                                        cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                     >  1:1886432/1‑74 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                 <  1:1982164/140‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                 <  2:2088608/140‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                 <  2:1048916/140‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                 <  2:879119/140‑1 (MQ=255)
                                                                                                          ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                 <  2:1156630/140‑1 (MQ=255)
                                                                                                           cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                <  2:508076/140‑1 (MQ=255)
                                                                                                           cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                <  2:2327373/140‑1 (MQ=255)
                                                                                                           cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                <  2:931891/140‑1 (MQ=255)
                                                                                                                   gagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTa                        <  1:1166244/140‑1 (MQ=255)
                                                                                                                   gagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTa                        <  2:1681527/140‑1 (MQ=255)
                                                                                                                       ggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGt                    <  1:1328801/140‑1 (MQ=255)
                                                                                                                       ggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGt                    <  2:540969/140‑1 (MQ=255)
                                                                                                                        gttgatGTTTTGGTAGGCGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc                   <  2:1529480/135‑1 (MQ=255)
                                                                                                                        gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                <  2:2161239/127‑1 (MQ=255)
                                                                                                                        gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc                   >  2:161634/1‑140 (MQ=255)
                                                                                                                        gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc                   <  2:841709/140‑1 (MQ=255)
                                                                                                                         tGGCTGTTTTTGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCa                  <  2:375679/140‑1 (MQ=255)
                                                                                                                          ggCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCGCCCCATTCCCGACAGTGAAGCGGGAGATTCTGATCTGTCTTTAATGTCat                 <  1:2020499/140‑1 (MQ=255)
                                                                                                                             tGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATc              >  1:1255080/1‑140 (MQ=255)
                                                                                                                             tGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATc              >  1:1499289/1‑140 (MQ=255)
                                                                                                                                 ttGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGa          >  2:2068808/1‑140 (MQ=255)
                                                                                                                                     tAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgc      >  2:1482007/1‑140 (MQ=255)
                                                                                                                                      aGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgct     <  2:1499289/140‑1 (MQ=255)
                                                                                                                                      aGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgct     <  2:1255080/140‑1 (MQ=255)
                                                                                                                                      aGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgct     <  1:945503/140‑1 (MQ=255)
                                                                                                                                      aGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgct     <  1:779044/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TCTCCGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCG  >  NZ_CP034943/1759024‑1759300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: