Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,759,191 A→C 5.5% P188P (CCA→CCC pksG → polyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,759,1910AC 5.5% 528.7 / 13.7 164P188P (CCA→CCCpksGpolyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG
Reads supporting (aligned to +/- strand):  ref base A (72/82);  new base C (2/7);  total (74/90)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 166 total aligned reads displayed.

CCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGAATACCAAA  >  NZ_CP034943/1759052‑1759324
                                                                                                                                           |                                                                                                                                     
ccAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGGTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCa                                                                                                                                       >  2:16881/1‑140 (MQ=255)
   gTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGa                                                                                                                                    >  2:1177921/1‑140 (MQ=255)
       tatTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCaa                                                                                                                                <  1:212130/140‑1 (MQ=255)
       tatTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCaa                                                                                                                                <  2:534104/140‑1 (MQ=255)
              cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGACTACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGTGGTGTTTCATATAGATCCAGGAGCACATGGCTa                                                                                                                         >  2:1081207/1‑140 (MQ=255)
              cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                                                         >  2:945503/1‑140 (MQ=255)
              cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                                                         >  2:498489/1‑140 (MQ=255)
              cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                                                         >  1:835048/1‑140 (MQ=255)
              cgTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTa                                                                                                                         >  2:741942/1‑140 (MQ=255)
               gTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTAt                                                                                                                        <  2:1170139/140‑1 (MQ=255)
                 tttttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTa                                                                                                                      <  2:1297697/140‑1 (MQ=255)
                 tttttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTa                                                                                                                      <  1:968418/140‑1 (MQ=255)
                 tttttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCAGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTa                                                                                                                      <  1:739638/140‑1 (MQ=255)
                    ttAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACgg                                                                                                                   <  1:568338/140‑1 (MQ=255)
                           cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCGGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTGTCAAATAGATCCAGGAGCAAAGGGCTATTACGGCTATGaa                                                                                                            >  2:1702990/1‑140 (MQ=255)
                           cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGaa                                                                                                            >  2:669224/1‑140 (MQ=255)
                           cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAAGGGCTATTACGGCTATGaa                                                                                                            >  2:858733/1‑140 (MQ=255)
                           cTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCAAGGAGCAAATGGCTATTACGGCTATGaa                                                                                                            >  2:923087/1‑140 (MQ=255)
                               gggCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGa                                                                                                        <  2:773620/140‑1 (MQ=255)
                               gggCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGa                                                                                                        <  2:864347/140‑1 (MQ=255)
                                         gCTTTAAGTGAAGACTGGTGTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                                                              <  2:2245168/140‑1 (MQ=255)
                                         gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                                                              <  1:2338773/140‑1 (MQ=255)
                                         gCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTg                                                                                              <  1:2154439/140‑1 (MQ=255)
                                          cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                                                             >  1:702079/1‑140 (MQ=255)
                                          cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                                                             >  1:407935/1‑140 (MQ=255)
                                          cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                                                             >  1:1421203/1‑140 (MQ=255)
                                          cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                                                             >  1:206123/1‑140 (MQ=255)
                                          cTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGc                                                                                             >  1:1616826/1‑140 (MQ=255)
                                              aaccgAAGATTGGTCTCATGCGGACCCGAGCGCCGGAGCGCGAGCGGTGGCCGATGTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCgcc                                                                                         <  1:1902706/136‑3 (MQ=255)
                                              aaGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTc                                                                                         <  1:271509/140‑1 (MQ=255)
                                              aaGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTc                                                                                         <  1:294482/140‑1 (MQ=255)
                                              aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAACTGGTTATTACGGCTATGACGTGATGGACACGTGCCgcc                                                                                         <  1:2188875/140‑3 (MQ=255)
                                              aaGCGAAGATTGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCgcc                                                                                         <  1:904877/140‑3 (MQ=255)
                                                   aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                                                    >  2:2227673/1‑140 (MQ=255)
                                                   aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                                                    >  2:1425047/1‑140 (MQ=255)
                                                   aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                                                    >  2:200790/1‑140 (MQ=255)
                                                   aaGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCAtt                                                                                    >  2:1785391/1‑140 (MQ=255)
                                                      aCTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTccc                                                                                 <  1:951481/140‑1 (MQ=255)
                                                      aCTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTccc                                                                                 <  1:631507/140‑1 (MQ=255)
                                                          gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCa                                                                                                                                 <  2:2403747/88‑1 (MQ=255)
                                                          gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACgg                                                                                                                   >  2:2029848/1‑102 (MQ=255)
                                                          gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACgg                                                                                                                   <  1:2029848/102‑1 (MQ=255)
                                                          gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACa                                                                             >  1:315041/1‑140 (MQ=255)
                                                          gTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACa                                                                             >  1:931891/1‑140 (MQ=255)
                                                               aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGaa                                                                        >  2:1045464/1‑140 (MQ=255)
                                                               aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGaa                                                                        >  1:1802270/1‑140 (MQ=255)
                                                               aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGaa                                                                        >  1:1363581/1‑140 (MQ=255)
                                                               aTGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGaa                                                                        >  2:1335020/1‑140 (MQ=255)
                                                                tGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAg                                                                       >  1:2229855/1‑140 (MQ=255)
                                                                     aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGACAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                                                  >  1:1574780/1‑140 (MQ=255)
                                                                     aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCggg                                                                   >  1:1915302/1‑139 (MQ=255)
                                                                     aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCggg                                                                   <  2:1915302/139‑1 (MQ=255)
                                                                     aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                                                  >  1:1250677/1‑140 (MQ=255)
                                                                     aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                                                  >  2:1328801/1‑140 (MQ=255)
                                                                     aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                                                  >  2:814424/1‑140 (MQ=255)
                                                                     aaCCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGga                                                                  >  2:248802/1‑140 (MQ=255)
                                                                     aaCCGAGCGACGGAGCGGGAGCGGTCGCTGTTTTGGTAGGGGAAAATCCCGAGGAGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATCGATACCTGCCGTCACATTCCCCACCGTGAAGCGGga                                                                  >  2:377780/1‑140 (MQ=255)
                                                                          aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                                             >  1:1358890/1‑140 (MQ=255)
                                                                          aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                                             >  1:2105540/1‑140 (MQ=255)
                                                                          aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTc                                                             >  1:1400725/1‑140 (MQ=255)
                                                                          aGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGCGAACATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGGTATGAAGTGATGGGTACCTGCCGGACCATTCCCGACCGTGAAGCTGGAGAtcg                                                             >  1:1358863/1‑138 (MQ=255)
                                                                            cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                                             >  1:45635/1‑74 (MQ=255)
                                                                            cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                                             >  1:45648/1‑74 (MQ=255)
                                                                            cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                                             <  2:45648/74‑1 (MQ=255)
                                                                            cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                                             >  1:1886432/1‑74 (MQ=255)
                                                                            cgcCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATg                                                                                                                             <  2:1886432/74‑1 (MQ=255)
                                                                              ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                                         <  2:2088608/140‑1 (MQ=255)
                                                                              ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                                         <  2:1064026/140‑1 (MQ=255)
                                                                              ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                                         <  1:1982164/140‑1 (MQ=255)
                                                                              ccGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAc                                                         <  1:547404/140‑1 (MQ=255)
                                                                               cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                                        <  1:200790/140‑1 (MQ=255)
                                                                               cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                                        <  1:2302178/140‑1 (MQ=255)
                                                                               cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                                        <  2:2327373/140‑1 (MQ=255)
                                                                               cGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGAcc                                                        <  2:508076/140‑1 (MQ=255)
                                                                                ggagcgggagcgGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCt                                                       <  1:1785391/140‑1 (MQ=255)
                                                                                ggagcgggagcgGTGGCTGTGTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCt                                                       <  2:2338966/140‑1 (MQ=255)
                                                                                       gagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTa                                                <  2:1462905/140‑1 (MQ=255)
                                                                                       gagcggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTa                                                <  1:1166244/140‑1 (MQ=255)
                                                                                           ggTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGt                                            <  1:1328801/140‑1 (MQ=255)
                                                                                            gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc                                           <  2:841709/140‑1 (MQ=255)
                                                                                            gTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTc                                           >  2:611080/1‑140 (MQ=255)
                                                                                              ggCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGATCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCGCCCCATTCCCGACAGTGAAGCGGGAGATTCTGATCTGTCTTTAATGTCat                                         <  1:1177921/140‑1 (MQ=255)
                                                                                                 tGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATc                                      >  1:1255080/1‑140 (MQ=255)
                                                                                                     ttGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGa                                  >  2:1651519/1‑140 (MQ=255)
                                                                                                     ttGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGa                                  <  2:1574780/140‑1 (MQ=255)
                                                                                                         tAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgc                              >  2:1482007/1‑140 (MQ=255)
                                                                                                          aGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgct                             <  1:779044/140‑1 (MQ=255)
                                                                                                          aGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgct                             <  1:2330313/140‑1 (MQ=255)
                                                                                                          aGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATtgct                             <  2:1499289/140‑1 (MQ=255)
                                                                                                             ggAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCg                          >  2:442288/1‑140 (MQ=255)
                                                                                                               aaaaTCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGaa                        <  2:2052930/140‑1 (MQ=255)
                                                                                                               aaaaTCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGaa                        <  2:363509/140‑1 (MQ=255)
                                                                                                                        aGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCttt               >  2:90461/1‑140 (MQ=255)
                                                                                                                             tttCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATAGCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGa          <  1:2380298/140‑1 (MQ=255)
                                                                                                                             tttCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGa          >  1:2034147/1‑140 (MQ=255)
                                                                                                                             tttCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGa          <  1:992867/140‑1 (MQ=255)
                                                                                                                              ttCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTTATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGaa         <  2:590976/140‑1 (MQ=255)
                                                                                                                              ttCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGaa         <  2:1611035/140‑1 (MQ=255)
                                                                                                                                     aGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGAATACCaaa  <  2:2322154/140‑1 (MQ=255)
                                                                                                                                     aGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGAATACCaaa  <  2:2222379/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
CCAGTGATATTTCGCGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGTGAAGACTGGTCTTATGCCGAACCGAGCGCCGGAGCGGGAGCGGTGGCTGTTTTGGTAGGGGAAAATCCCGAGGTGTTTCAAATAGATCCAGGAGCAAATGGCTATTACGGCTATGAAGTGATGGATACCTGCCGTCCCATTCCCGACAGTGAAGCGGGAGATTCTGACCTGTCTTTAATGTCATATCTGGATTGCTGCGAACAGGCCTTTCTGGAATACCAAA  >  NZ_CP034943/1759052‑1759324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: