Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 704,350 Δ1 bp intergenic (+172/‑12) EO946_RS03650 → / → EO946_RS03655 CamS family sex pheromone protein/phosphotransferase enzyme family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943704,3480A.99.6% 1163.3 / ‑2.4 233intergenic (+170/‑14)EO946_RS03650/EO946_RS03655CamS family sex pheromone protein/phosphotransferase enzyme family protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (110/122);  total (111/122)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 259 total aligned reads displayed.

GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGA  >  NZ_CP034943/704209‑704483
                                                                                                                                           |                                                                                                                                       
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         <  2:2566613/140‑1 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCCATAGCaaagaa                                                                                                                                        <  2:509161/140‑1 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                        >  1:1786655/1‑140 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                        <  1:3785999/140‑1 (MQ=255)
 cacaAGGGTTTTTTTAGGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                        <  1:683099/140‑1 (MQ=255)
  acaattgTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCTAGACACAAATCAGGGTGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:3314137/134‑2 (MQ=255)
  acaAGTGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:233492/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:3696100/140‑2 (MQ=255)
  acaAGGGTATTTTTATTTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:2245239/140‑2 (MQ=255)
      gggTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATCGCAAAGAAGGGAg                                                                                                                                  >  2:2676497/1‑140 (MQ=255)
      gggTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAg                                                                                                                                  >  1:1273101/1‑140 (MQ=255)
      gggTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAg                                                                                                                                  >  1:3721682/1‑140 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATATCAAAGAAGGGAGTTAGa                                                                                                                             <  1:3665085/140‑1 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                             >  2:1621114/1‑140 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                             >  1:578158/1‑140 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                             >  1:3454076/1‑140 (MQ=255)
            ttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGAc                                                                                                                            <  1:1680729/140‑1 (MQ=255)
                 tGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCa                                                                                                                       >  1:1168015/1‑140 (MQ=255)
                   ttACTATTAACAGTAATAGGAAAAAGATGTATTTTTGCTTCTGCCGATACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATa                                                                                                                     <  2:3863270/140‑1 (MQ=255)
                             cAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCaa                                                                                                           <  2:416522/140‑1 (MQ=255)
                                gAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCaaaaa                                                                                                        >  2:2174674/1‑140 (MQ=255)
                                 aaTAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAAc                                                                                                       <  2:3348818/140‑1 (MQ=255)
                                   tAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACat                                                                                                     >  1:752409/1‑140 (MQ=255)
                                    aGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACata                                                                                                    <  1:1879808/140‑1 (MQ=255)
                                          aaGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGTGGAGGTATGTGGATGCTGGAACGCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCAt                                                                                              >  2:697758/1‑140 (MQ=255)
                                          aaGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCAt                                                                                              >  2:912281/1‑140 (MQ=255)
                                          aaGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCAt                                                                                              <  1:1537380/140‑1 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             >  2:4065355/1‑140 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             >  2:3903849/1‑140 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             >  2:756308/1‑135 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        <  1:4261991/140‑1 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        >  1:3033564/1‑140 (MQ=255)
                                                      tGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATTCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGtt                                                                                  <  1:1766636/140‑1 (MQ=255)
                                                      tGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:1861173/88‑2 (MQ=255)
                                                          tCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTgg                                                                              >  2:3049403/1‑140 (MQ=255)
                                                          tCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTgg                                                                              >  2:2644433/1‑140 (MQ=255)
                                                             gCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCag                                                                           >  2:1524450/1‑140 (MQ=255)
                                                             gCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCag                                                                           <  2:4224257/140‑1 (MQ=255)
                                                             gCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCag                                                                           >  2:2354601/1‑140 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                   <  1:4054705/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTAGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                   <  1:2677225/140‑1 (MQ=255)
                                                                      caAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGa                                                                  <  1:2346510/140‑1 (MQ=255)
                                                                              ggggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATAc                                                          >  1:2211815/1‑140 (MQ=255)
                                                                              ggggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATAc                                                          >  1:2483839/1‑140 (MQ=255)
                                                                               gggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACg                                                         <  1:4229469/140‑1 (MQ=255)
                                                                               gggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGATTTGGCAGAGGCTGCGATGAAATACg                                                         <  1:3363309/140‑1 (MQ=255)
                                                                                 gAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGt                                                       >  1:3957222/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACg                                                         >  1:332379/1‑136 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                     >  2:1585308/1‑140 (MQ=255)
                                                                                   ggTATGGTGATGATGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAATGAAGGGAGTTAGACGTGCATAAAGAAAAAAAAAACAAAATTCATGAAGATCAGGTTTTGGCAGAGCCTCCGAAGAAATACGGttt                                                     >  2:134960/1‑140 (MQ=255)
                                                                                    gTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTtc                                                    >  2:2978196/1‑140 (MQ=255)
                                                                                     tATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTtct                                                   >  1:3152055/1‑140 (MQ=255)
                                                                                        gTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaa                                                <  2:578158/140‑1 (MQ=255)
                                                                                        gTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaa                                                <  1:852844/140‑1 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  2:2490737/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  1:2640183/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  1:3312416/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  1:214182/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  1:2080647/1‑140 (MQ=255)
                                                                                               cTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCa                                         <  2:651421/140‑1 (MQ=255)
                                                                                               cTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCa                                         >  1:159501/1‑140 (MQ=255)
                                                                                                 ggAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAgg                                       >  1:3525641/1‑140 (MQ=255)
                                                                                                 ggAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAgg                                       >  2:1560591/1‑140 (MQ=255)
                                                                                                   aaCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGACGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTg                                     <  1:4167428/140‑1 (MQ=255)
                                                                                                    aCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGc                                    <  2:3190521/140‑1 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaa                                                >  2:3811248/1‑123 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCCGGTGCGtttt                               >  1:418405/1‑140 (MQ=255)
                                                                                                          gtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTc                              >  1:1045463/1‑140 (MQ=255)
                                                                                                             aaaaCAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaaa                                               >  2:3231130/1‑120 (MQ=255)
                                                                                                                  aatcaatATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGAt                      >  2:981074/1‑140 (MQ=255)
                                                                                                                     caatATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCg                   <  1:1583853/140‑1 (MQ=255)
                                                                                                                       atatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGa                 <  1:3902882/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                <  1:2354601/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                <  1:2745460/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGCAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATGTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:32063/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:1257631/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  2:1313312/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCCGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  2:221986/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCACAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:2158728/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAACACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:2835267/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAACCATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:80163/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:1172857/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:106377/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            >  2:1219730/1‑140 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:3525641/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1560591/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:2224634/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:4249352/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAACAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:1022839/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGGTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:242589/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          <  1:3851123/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          <  2:3590247/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:1311818/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:1291409/1‑140 (MQ=255)
                                                                                                                                  aTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCCGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGtat      >  1:96904/1‑140 (MQ=255)
                                                                                                                                  aTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGtat      >  1:1499400/1‑140 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa  <  1:2922632/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa  <  2:1273101/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa  <  1:775058/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa  <  2:344235/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGA  >  NZ_CP034943/704209‑704483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: