Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943886,7510GC25.1% 414.1 / 21.4 196S46C (TCT→TGT) EO946_RS04490MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (96/41);  new base C (0/46);  total (98/98)
Fisher's exact test for biased strand distribution p-value = 2.61e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 208 total aligned reads displayed.

AGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAT  >  NZ_CP034943/886612‑886889
                                                                                                                                           |                                                                                                                                          
       aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTGCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTGAGGCCCCCTGACCCCCAGGCAGGAGACACGGc                                                                                                                                     >  1:2802319/1‑140 (MQ=255)
       aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGc                                                                                                                                     >  1:714619/1‑140 (MQ=255)
             aaCAGGCAAACCTAAAGCCTAACATGTTTGTTGCTGCCCTAGCGGTCGCAAAGCTTTCCCAGGATCGCCACACTCACGCTAAGGCCGAGCGTATATAGGGTAATCCCCCATGACACCCAGTACGGAGAACCGGCAAACGa                                                                                                                               <  2:2451967/140‑1 (MQ=255)
                  gCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGt                                                                                                                          >  2:386781/1‑140 (MQ=255)
                  gCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGt                                                                                                                          >  1:3046931/1‑140 (MQ=255)
                  gCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCACGAAGGAGAAACCGCAAACGACTCGt                                                                                                                          >  1:3049588/1‑140 (MQ=255)
                       ccTCATGGACAAAATGTTTCTTTCTGCCTTCGGGGGCGCAACGCTTCCCGACGATCGGCACACTGCCGATACGGCCGAGCTTATAGAGGGTAAGGCCCCATGACCCCCAGGACGGAGAAACGGCAAACGACTCGTTTATc                                                                                                                     <  2:777377/140‑1 (MQ=255)
                            atcacaaaaaGTTTCTTTCTGCGGTAGCGGTCGGAAAGCTTTCCGACGATCGGAACACTGCCGCGACGGCCGAGCGTATAGAGGGTAAGGCCCCATGACCCCCAGGACGGAGAACCGCCAAACGACTCGTTTATCgttgt                                                                                                                <  2:4125582/135‑1 (MQ=255)
                            aTGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGACGGAGAAACGGCAAACGACTCGTTTATCgttgt                                                                                                                >  2:2763002/1‑140 (MQ=255)
                               agaAAAATTTTCTTTCGGCGGTCGGGGTCGTAAAGCTTTCCGAGGATCGGCACACTGACGCTAAGGCCGAGCGTATAGCGGGTAATGCCCCATGCCCCCCAGGACGGAGAAACGGCAAACGACTCGTTTATCGTTGTTaa                                                                                                             <  2:3049588/140‑1 (MQ=255)
                                 aaaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTGTATCGTTGTTAAag                                                                                                           >  2:944040/1‑140 (MQ=255)
                                  aaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGCCGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCCAACGACTCGTTTATCGTTGTTAAagc                                                                                                          >  1:3944871/1‑140 (MQ=255)
                                                  cttAGCGTGCGCAAAGCTTTCCCACGATCGGCACACTGCCGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGCAAGGACAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCg                                                                                          <  2:1661829/138‑1 (MQ=255)
                                                  cgtcggggTCGCAAAGCTTCCCGACGATCGGCACACTGACGCTATGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCCGGACGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCg                                                                                          <  2:714619/134‑1 (MQ=255)
                                                       gggTCGGAATGCTTTCCGACGATCGGCACACTGACGCTACGGCCGCGCTTTTAGCGGGTAATGCCCCATGCCCCCCAGGAAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATc                                                                                     <  1:2039434/139‑1 (MQ=255)
                                                            ttatcgatttCCGAGGATCGGCACACTGACGCTAAGGCCGCGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAGGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  1:2930960/133‑1 (MQ=255)
                                                            tgAACGCTTGCCCACGATCGGCACACTGACGCAACGCCCGAGCGTTTAGAGGGTAATGCCCAAGGACCCCCCGGAAGGAGAAAGGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  1:3189084/139‑1 (MQ=255)
                                                            gcaatgCTTTCCCCCGAGCGGCACCCTGACGCTAAGGCCGAGCGTATAGAGGTTATTGCCCCAGGACCCCCAGGACGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgca                                                                                <  2:24879/135‑2 (MQ=255)
                                                            gcaacgCTTCCCGACGATCGCCACCCGCCCGCTAAGGCCGAGCGTATAGTGGGTAAGCCCCCATGCCCCCCAGGAAGCACAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  1:1083616/135‑1 (MQ=255)
                                                            gaaatgCTTTCCGACGATCGGCAAACGGACGGTAAGGCCGAGCTTATAGTGGTTAAGGCCCCTGCACCCCCAGCCAGTACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  2:3647927/135‑1 (MQ=255)
                                                            ccaaaGCTTTCCCACTATCGCCACACTGACGCTACGCCCGAGCTTATACAGGGTAAGCCCCAATGACCCCCAGGAAGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  2:3089703/138‑1 (MQ=255)
                                                             cactgCTTCCCGACTATCGGCACACCGACGCTCAGCCGGGTCGTTGAGAGGGAAATGCCACAGGCCCCCCCGGACGCACAACCGGCCCACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcgc                                                                               <  1:3134910/136‑1 (MQ=255)
                                                             caaaGCTTCCCTCCGATCGGCAACCTGACGATACGGCCGAGGTTTTAGAGGGTAATGCCCAATGACCCCCAGGAAGTACAACCGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcgc                                                                               <  1:2211750/139‑1 (MQ=255)
                                                                    ttCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATa                                                                        >  1:3089703/1‑140 (MQ=255)
                                                                      cccccgaTCGCAACACTGACGCTCAGGCCGTGCGTATAGAGGTTAATCCCCCATGACCCCCAGTAAGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  1:212829/136‑1 (MQ=255)
                                                                        tacgaTCGGCACACTCACTCTAAGGCCGTGCTTATAGAGGTTAATGCCCCATGACCCCCAGGAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAaca                                                                    <  2:2401015/139‑1 (MQ=255)
                                                                           gaTGGGCACCCTGACGCTACGGCCGCGCTTATAGAGGGTAATGCCCAATGACCCCCAGCATGGACAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:2681647/140‑1 (MQ=255)
                                                                           gaTCTGCACACTGCCGATAAGCCCGAGCGTTTAGAGGTTATTGCCCAAGCACCCACAGTACCGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:3892834/140‑1 (MQ=255)
                                                                           gaTCGGCACACTGCCGCTAAGGCCGAGCGTATAGAGGTTAATGCCCCATGACCCCCAGGAAGGAGAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  2:3142811/140‑1 (MQ=255)
                                                                           gaTCGGAACAGTGACGCGATGGCCGCGGTTTTAGTGGTTATGGCCCAATCACCCCCGGTAAGGACAAACGCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:3881098/140‑1 (MQ=255)
                                                                                gCACACTGCCGCTACGGCCGAGCGTATAGATGTTAATGCCCCATGACCCCATGGAAGCAGAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATc                                                            <  1:60141/140‑1 (MQ=255)
                                                                                        aCGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTCTCCAGCGCAGCCATAAACACACCAATCGTAAACAg                                                    >  2:3261527/1‑140 (MQ=255)
                                                                                        aCGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAg                                                    >  2:2120471/1‑140 (MQ=255)
                                                                                        aCGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAg                                                    >  2:3489339/1‑140 (MQ=255)
                                                                                         cggtAAGGCCGCGGGTATAGAGGGTAATGCCCCAGGACCCCCAGCAAGCACAACGGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAgg                                                   <  2:2583638/137‑1 (MQ=255)
                                                                                           cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  2:3036735/1‑140 (MQ=255)
                                                                                           cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  2:2976274/1‑140 (MQ=255)
                                                                                           cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  2:2711474/1‑140 (MQ=255)
                                                                                           cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGGTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTACTCCAGCGCAGCCCTAAACACACCAAGCGTACACAGGCt                                                 >  2:2526373/1‑140 (MQ=255)
                                                                                             aaGGCCTAGCTTATAGAGGTTAATCCCCATTGACCCCCCGCAAGTTTAACCGCAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATACAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGa                                               <  2:1771048/140‑1 (MQ=255)
                                                                                              aGGCCGAGCGTATATAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAATCAGCAGAAATAATTCAGTTATCCAGCGCAGCCCTCAACACACCAATCGTAAACAGGCTGAt                                              >  2:3704449/1‑140 (MQ=255)
                                                                                                  cGAGCGTATTGTGGTTATGCCCCCATGCCCCCCAGGCCGGACAACGGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                          <  1:386780/140‑1 (MQ=255)
                                                                                                  agAGCTTATAGAGGGTAATCCCCCATGACCCCCAGGAAGCAGAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                          <  2:652384/139‑1 (MQ=255)
                                                                                                    aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAATGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCCTAAACAGGCTGATCGCCCa                                        >  2:3858384/1‑140 (MQ=255)
                                                                                                    aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  1:1306599/1‑140 (MQ=255)
                                                                                                    aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  2:293470/1‑140 (MQ=255)
                                                                                                    aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  2:3748843/1‑140 (MQ=255)
                                                                                                    aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  2:1625007/1‑140 (MQ=255)
                                                                                                    aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCAGAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  2:2872376/1‑140 (MQ=255)
                                                                                                      cGTATCGCGGGCACTCCCCCAGGCCCCCCCGGCAGTACAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACt                                      <  1:2276806/140‑1 (MQ=255)
                                                                                                         ataGAGGGTAATGCCCCATCACCCCCTGGAAGGACAACCGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACtttt                                   <  1:944040/140‑1 (MQ=255)
                                                                                                           cgcgggTAATGCCCCAGCACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATACTTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                 <  2:3232351/137‑1 (MQ=255)
                                                                                                           agagGGTACTGCCCCATCACCCCCAGGAAGTACAACGGCCACACGCCTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATACACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                 <  1:1636581/140‑1 (MQ=255)
                                                                                                               ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                >  1:984459/1‑89 (MQ=255)
                                                                                                               ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCATCGCATCCATAAACACACCAATCGTAAACAGGCTGATAGCCCACTTTTGGCtgt                             >  2:3581810/1‑138 (MQ=255)
                                                                                                               ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:2824942/1‑140 (MQ=255)
                                                                                                               ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:2076338/1‑140 (MQ=255)
                                                                                                               ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:3666189/1‑140 (MQ=255)
                                                                                                               ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:540681/1‑140 (MQ=255)
                                                                                                               ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:2886078/1‑140 (MQ=255)
                                                                                                                gTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCTCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                            >  1:3231793/1‑140 (MQ=255)
                                                                                                                gTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                            >  1:1121147/1‑140 (MQ=255)
                                                                                                                gTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                            >  1:3844848/1‑140 (MQ=255)
                                                                                                                gTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                            >  1:484559/1‑140 (MQ=255)
                                                                                                                 tAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTc                           >  2:14783/1‑140 (MQ=255)
                                                                                                                   aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                         >  2:3831042/1‑140 (MQ=255)
                                                                                                                   aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                         >  1:3477252/1‑140 (MQ=255)
                                                                                                                   aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCAGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTGTTCTg                         >  2:2013347/1‑140 (MQ=255)
                                                                                                                    tGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                        >  2:623324/1‑140 (MQ=255)
                                                                                                                    tGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                        >  2:86230/1‑140 (MQ=255)
                                                                                                                     gCCCCATGACCCCCCGGACGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  2:3331042/140‑1 (MQ=255)
                                                                                                                     gCCCCATGACCCCCCGGACGCAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  1:3863905/140‑1 (MQ=255)
                                                                                                                     gCCCCATGACCCCCCGGAAGGATAACCGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  2:1306599/140‑1 (MQ=255)
                                                                                                                     gCCCCATCACCCCCCGGACGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  1:649550/140‑1 (MQ=255)
                                                                                                                     gCCCCATCACCCCCCCGAAGCACAACCGCAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCCTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  1:2996254/140‑1 (MQ=255)
                                                                                                                     gCCCCAGGCCCCCCAGTACGGACACACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  1:919512/140‑1 (MQ=255)
                                                                                                                     gCCCCAGCACCCCCAGTAAGCAGAAACGCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  1:1787553/140‑1 (MQ=255)
                                                                                                                       ccccgcccccccAGCCAGCACAACCTGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAAGAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGctct                     <  2:592109/133‑1 (MQ=255)
                                                                                                                         cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                          >  2:3224704/1‑117 (MQ=255)
                                                                                                                         cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   >  1:3194798/1‑140 (MQ=255)
                                                                                                                         cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   >  1:2851616/1‑140 (MQ=255)
                                                                                                                         cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   >  1:847753/1‑140 (MQ=255)
                                                                                                                         aatcaCCCCCAGGAAGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   <  2:3477252/136‑1 (MQ=255)
                                                                                                                           tcaCCCCCCGGACGCACACACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACCCCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 <  1:1315033/138‑1 (MQ=255)
                                                                                                                           tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                       <  2:299907/102‑1 (MQ=255)
                                                                                                                           tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 >  1:496442/1‑140 (MQ=255)
                                                                                                                           tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 >  2:1432731/1‑140 (MQ=255)
                                                                                                                            gcccccccGGAAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                <  1:2503210/138‑1 (MQ=255)
                                                                                                                            gACCCCCAGTCCGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                <  1:2655381/140‑1 (MQ=255)
                                                                                                                            gACCCCCAGGAAGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                <  1:1892307/140‑1 (MQ=255)
                                                                                                                            gACCCCCACGAAGCACACCCGCAACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                <  2:1026342/140‑1 (MQ=255)
                                                                                                                            cccccccAGCAAGGACAACCGCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                <  2:496442/138‑1 (MQ=255)
                                                                                                                                cccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCt            >  2:2171037/1‑140 (MQ=255)
                                                                                                                                cccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTGTTCTGCTCTGTCAATGCt            >  1:539973/1‑140 (MQ=255)
                                                                                                                                cccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCCTTATCCAGCGCAGCCATAAACACACCAAGCGTAAACAGGCTGATCGCCCACTGTTGGCTTTTCTGCTCTGTCAATGCt            >  1:2298870/1‑140 (MQ=255)
                                                                                                                                    ggaaggaGAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAg        <  1:3748843/140‑1 (MQ=255)
                                                                                                                                        ggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACat    >  1:4076566/1‑140 (MQ=255)
                                                                                                                                         gagaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                 >  1:3349412/1‑110 (MQ=255)
                                                                                                                                         gagaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                         <  2:3925513/118‑1 (MQ=255)
                                                                                                                                          agaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACatag  >  2:4043083/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAT  >  NZ_CP034943/886612‑886889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑