Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,366,1150GC31.0% 241.3 / 24.3 142E205Q (GAA→CAA) EO946_RS07135PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (51/38);  new base C (0/40);  total (52/90)
Fisher's exact test for biased strand distribution p-value = 8.25e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 144 total aligned reads displayed.

CAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACTCATACAA  >  NZ_CP034943/1365977‑1366249
                                                                                                                                           |                                                                                                                                      
caaAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAaaga                                                                                                                                       >  1:393750/1‑140 (MQ=255)
     aaCACCTATTACTAAAAAATAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGG‑ACAGCGGATTACGCTGATCTCTAAAAAAGTGTGGGCACTATAATCTTTTAAGTGGTTTTTTATCTTCGGTTTTATAAAGAAGCGa                                                                                                                                  <  2:2216620/140‑1 (MQ=255)
         cagATTACTAAAAACGAAGCCAAAACCATTGCTGAGCGATAACCGGGCGGC‑ACAGCGGATGACGCAGATCTCTAAAAAAGTGAGGGCACTCTAATCTTTGATGTGGATGTTTATATTCCGTATTATAAATAAGCGACtgt                                                                                                                              <  2:2335420/138‑1 (MQ=255)
          cGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACtgtg                                                                                                                             >  1:1018562/1‑140 (MQ=255)
                ccaaaaaaGACCCCAAACCCTTTGGTGAGCTAGAAACGGGCGGC‑ACAGCGTATGACGCTTATCTCTAAAAAAGTGAGGGCACTCTAATCTTTTAAGTGTTTGTTTATCTTCCGGATTATAAAGAAGCGACTGTGAAAAtt                                                                                                                       <  2:2872854/138‑1 (MQ=255)
                acaaaaaaCAAGCCAAAACCATTGCTTAGCGAGAAACGGGCGGG‑ACAGCGGATTACGCATATCTCTAAAAAAGTGAGGGCACTCAAATCTTTGAAGTGGATGTTGATCTTCCGCATGATAAAGAAGCGACTGTGAAAAtt                                                                                                                       <  2:1018562/138‑1 (MQ=255)
                        gaaccaaaaaCCTTTGCTGAGCGAGAAACGGGCGGG‑ACAGCGTTTGACGCATATATCGAAAAATTTGAGGGCACTCTCATCTTTTAAGTGTATGTTTATCTTCCGTATTATAAAGAAGCGACTGTGAAAATTAATGCCTa                                                                                                               <  2:515373/134‑1 (MQ=255)
                                  cctgtgctgcGCGACAACCGGGGGGC‑ACAGCGGTTGCCCCAGACATCTAAAAAAGTTAGGGCAATGTCATCTTTGATGTGTTTTTTTATCTTCCGGATTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAg                                                                                                     <  1:732113/136‑1 (MQ=255)
                                    aTTGCTGAGCGAGAAACGGGCGGG‑AAAGCGTATGACGCATATCTCTAAAAAAGTGAGGGCACTCTAATCTTTTAAGTGTATGTTGATCTTCCGGATTATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGt                                                                                                   <  2:1860417/140‑1 (MQ=255)
                                        gtGAGCGAGAAAGGGGGGGG‑ACTGCTGATGACGATTATCTCGAAAAATTTGAGGGAAGTCTCATCTTTGATGTGGATTTTGATGTTCCGTTTGATAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa                                                                                               <  2:2998455/139‑1 (MQ=255)
                                        cTGCGCGAGAACCGGGGGGT‑ACAGCGGAGGACGCAGATCTCTAAAAATTTGAGGGCACTCTCATGTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa                                                                                               <  2:2163326/140‑1 (MQ=255)
                                        cTGCGCGAGAAACGGGCGGG‑ACAGCGTTTGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGATGTGGATGTTGATCTTCGGCTTTATAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa                                                                                               <  2:4075533/140‑1 (MQ=255)
                                        cTGAGCGATAAACGGGGGGC‑ACAGCGGTTGACTCAGATCTACAAAAATGTGAGGCCACTCTCATCTTTGATGTGGTTTTTGATCTTCCGGATTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa                                                                                               <  1:1116654/140‑1 (MQ=255)
                                        cTGAGCGACAACGGGGGGGG‑ACCGCGTATGACGCAGATCTCCAAAAAAGTGAGGCCACTCTCATCTTTGATGTGGATGTTTATCTCCCGTATTATAACCACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa                                                                                               <  1:3110280/140‑1 (MQ=255)
                                          gtgctataaaCGGGCGGC‑ACAGCGCATGACGCACATCTCTAAAAATGCGAGGGCACTCTCATTTTTGATGTGGTTGTTGATCTTCCGGTTGATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCaaa                                                                                             <  2:3651662/133‑1 (MQ=255)
                                          gAGCGACAAACGGGCGGG‑ACAGGGGATGCCGCAGATCTCGAAAAACGTGAGGGCACTCTCATCTTTTATGTGGATGTTTATCTACCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCaaa                                                                                             <  2:213452/140‑1 (MQ=255)
                                           aGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCCTCTTTGAAGTGGATCTTGATCTTCCGGATGATAAAGAAGCGACTCGGAAAATTAATGCCTACACCGGAAAGGTTGCAAAc                                                                                            >  2:2750454/1‑140 (MQ=255)
                                           aGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGACAGGTTGCAAAc                                                                                            >  2:3844242/1‑140 (MQ=255)
                                           aGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAAc                                                                                            >  2:2488728/1‑140 (MQ=255)
                                           aGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAAc                                                                                            >  1:1860417/1‑140 (MQ=255)
                                           aGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAAc                                                                                            >  1:2611219/1‑140 (MQ=255)
                                            gctctaaaCGGCGGGC‑ACTGCGGATGACCCATATCTCTAAAAAATTGCGGGAACTCTAAACTTTTATGTTGATGTTGATATTCCGGTTTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  2:3834219/135‑1 (MQ=255)
                                            gctataaaCGGGGGGG‑ACAGCGCGTGACCCATATTTGCAAAAAAGGGATGTCACGCCAATATTTTATGTGTTTGTTTATCTACGTGTTTATAAACACGCGACTGTGAAAATTAATGACTACACCGGACAGGTTTCAAACa                                                                                           <  2:3587040/135‑1 (MQ=255)
                                            gCGAGAACCGGGGGGG‑ACCGCGTAGGCCGCTGATCTCGAAAAATGTGAGCGCACTCTCATCTTTTATTTGGATGTTTATCTTCGGGATTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  2:1303322/140‑1 (MQ=255)
                                            gCGAGAACCGGGGGGC‑ACCGGGTATGACGCAGATCTCAAAAATCTTGAGGGAAATATATTATTTGATGTGTTTTTTCATGTTCGGTATTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  1:3545610/140‑1 (MQ=255)
                                            gCGAGAACCGGGCGGG‑ACAGGGTATGACGAATATCTCGAAAAAAGTGAGGGCACTCTAATTTTTGATGTGTTGGTTTATCTTCCGGATGATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  2:393750/140‑1 (MQ=255)
                                            gCGAGAACCGGGCGGC‑ACCGCGTATCACGCATATCTCCAAAAAAGTGAGGCCACACTAATATTTCAAGTGGATTTTGATCTTCCGCATCATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  1:2987767/140‑1 (MQ=255)
                                            gCGAGAACCGGGCGGC‑ACCGCGGAGCCCCCAGATATCCAAAAACGTTAGGCCACCCAAATTTTTGACGGGTATTTTTATCTCCCGGATTAAAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  1:1701950/140‑1 (MQ=255)
                                            gCGAGAAACGGGGGGT‑ACAGCGGATGACGCAGATCTCTAAAAAAGTGAGGGCACTCTCATATTTGAAGTGGTTTTTGATATTCCGTATCATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  2:288953/140‑1 (MQ=255)
                                            gCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGACGTGTATTTTGATCTTCCGTATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  2:73993/140‑1 (MQ=255)
                                            gCGACAAAGGGGGGGC‑ACCGCGTCTGACGCAGATCACCAAAAATGTGAGGGCACTCTAATCTTTGAAGTGGTTGTTGATGTTCCGGAGTATAAACCCGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  1:733093/140‑1 (MQ=255)
                                             gctagaaCGGGGGGGCACAGGGTATGACGAATATCTCTAAAAAAGTGAGGGCACTCTCATCTTTGAATTGTTTGTTTATCTTCCGTAGTATAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa                                                                                           <  2:447949/135‑1 (MQ=255)
                                                gaAAGGGGCGGG‑ACCGCGGATGACGCAGATCTCTAAAAATGTGAGGGCACTCTAATATTTGACGTGTATTTTGATCTTCGGGATGATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATtgt                                                                                       <  1:3098174/140‑1 (MQ=255)
                                                    cggggggg‑ACAGCGGATCACCCATATCTCCAAAACAGTGAGGGCACTCTAATTTTTGATGTGTATTTTGATCTACGGGATTATAACCACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTAt                                                                                   <  1:3570951/140‑1 (MQ=255)
                                                    cgggcggg‑ACCGCGTATCACGAATATCTCTAAAAAAGTGAGGGCACTATATTCTTTGATGTGGTTGTTGATCTACCGTATTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTAt                                                                                   <  1:1712585/140‑1 (MQ=255)
                                                    cgggcggc‑ACAGGGTATGCCGCATCCCACCAAAAAAGTGTGGCCCCACTAATATTTGAATGGGATGTTGATATTCCGGATGAAAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTAt                                                                                   <  1:3941935/140‑1 (MQ=255)
                                                      ggcggg‑ACCGCGGTTGACGCAGCTCTCTAAAAAAGTGAGGCCACTATCATCTTTGATTGGGTTGTTGATCTTCCGGATGATAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGa                                                                                 <  2:1426338/140‑1 (MQ=255)
                                                       gcggg‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAg                                                                                >  2:4197667/1‑140 (MQ=255)
                                                          ggcaccgCGGATGCCGAAGATCTCTAAAAATGTGAGGCCACTCTCATTTTTTATGTGTTTTTTTATGTTCGGTATTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATCGTGTATGAGGa                                                                              <  1:83410/134‑1 (MQ=255)
                                                          ggcaCAGCGTATGACGAAGATCTCAAAAAAATTGAGGCCACTATAATCTTTGATGTGGTTGTTGATCTCCCGGTTTAAAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGa                                                                              <  1:2853937/137‑1 (MQ=255)
                                                          ggcaCAGCGTATGACGAACATATATAAAAAAGTGAGGCCAATATCTTATTTGATGTGTTTGTTGATCTTCCGTATTAAAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGa                                                                              <  1:2480500/137‑1 (MQ=255)
                                                          gg‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGAc                                                                             >  1:3729623/1‑140 (MQ=255)
                                                               cgCGGATGACCCAGATCTCCAAAAATGTGAGGGCACTCTCATTTTTGAAGTGTATGTTTATCTTCCGTATCATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGAc                                                                         <  2:1160756/139‑1 (MQ=255)
                                                               aGCGGAGCACCCACATCTCCACAAATGTCACGCCACGCACATATTTGACCTGGATGTGCATCTCCCGTATGAGAAAGCAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGAc                                                                         <  2:1634526/140‑1 (MQ=255)
                                                                 cGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACgg                                                                       >  2:4152278/1‑140 (MQ=255)
                                                                  ggttGACGCAGATCTCTAAAAATGTGAGGGCACTGCCTTCTTTGAAGTGGATGTTGATCTGCCGGTTTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACggg                                                                      <  2:572900/137‑1 (MQ=255)
                                                                  ggATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTTAAGTGGATGTTGATCTTCCGGATTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACggg                                                                      <  2:13324/140‑1 (MQ=255)
                                                                    aTGACGCATAGCACCAAAAATGTTAGGGCACTCAATTTTTTCAATTGGATGTTCATCTTCCGTATTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGtt                                                                    <  1:2198152/140‑1 (MQ=255)
                                                                     ggACCCAGATCTCAAAAAATGTCAGGGCCAGCTCATCTTTGAGGGTTTTTTTCAGCGTCCGGATCAAAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTtc                                                                   <  1:2360943/139‑1 (MQ=255)
                                                                           aGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGTAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGACAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCtcatca                                                             >  1:1810603/1‑140 (MQ=255)
                                                                           aGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCtcatca                                                             >  1:977765/1‑140 (MQ=255)
                                                                           aGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCtcatca                                                             >  1:3877839/1‑140 (MQ=255)
                                                                           aGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGGGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCtcatca                                                             >  2:1095525/1‑140 (MQ=255)
                                                                           aGATCTCGAAAAAAGTGAGCGCACTCTCATCTTTGAACTGGATGTTGAACTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTTACGGGTTCtcatca                                                             >  2:3956556/1‑140 (MQ=255)
                                                                             aTCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCaaa                                                           >  1:2059605/1‑140 (MQ=255)
                                                                                 ccaaaaaaGTCAGGCCACACACATATTTGACGTGGATGTTCCTGTCCCGGATTATAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATtct                                                       <  1:611924/138‑1 (MQ=255)
                                                                                  gAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATtctc                                                      >  1:130558/1‑140 (MQ=255)
                                                                                  gAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATtctc                                                      >  2:2802786/1‑140 (MQ=255)
                                                                                  gAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATtctc                                                      >  1:1138629/1‑140 (MQ=255)
                                                                                           gcgggCCCTCTAATCTTTGTTGTGGATTTTTATCTTCCGGATTATAAAGAAGCGACTGTGAAAATTAATGACTACACCTGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTtctc                                             <  2:2931860/138‑1 (MQ=255)
                                                                                           gAGGGCACTGTCTTATTTTAAGTGTTTTTTGATGTTCCTGTTTATAAAGTATCGACTGTGAAAATTTATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTtctc                                             <  2:3235716/140‑1 (MQ=255)
                                                                                           gAGGGCACTATCATATTTGAATGGTATGTTGATATTCCGGTTGATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTtctc                                             <  2:3495642/140‑1 (MQ=255)
                                                                                            aGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTtctct                                            >  1:2544846/1‑140 (MQ=255)
                                                                                                aaCTCTAATATTTGATGTGGTTTTTTATCTCCCGGTTGATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGtt                                        <  1:3789677/139‑1 (MQ=255)
                                                                                                 aCTCTCATATTTGAAGGGGATGTTGTTCGTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGttt                                       <  2:3523056/140‑1 (MQ=255)
                                                                                                   tatcttCTTTGATGTGTATGTTGATCTTCCGGATCATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTc                                     <  2:3710450/135‑1 (MQ=255)
                                                                                                   tataaTCTTTGAAGTGGATTTTTATCTCCCGGATTATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTc                                     <  1:2444471/136‑1 (MQ=255)
                                                                                                     ttaTCTTTTAAGTGGTTTTTTATCTTCCGTATGATAACCACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCt                                   <  1:318972/138‑1 (MQ=255)
                                                                                                     tcATCTTTTATGTGTATGTTGATCGTCCGGATGATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCt                                   <  2:2360763/140‑1 (MQ=255)
                                                                                                     ccATCTTTGACGTGGATGTTGATCTTCCGTATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCt                                   <  1:3016859/139‑1 (MQ=255)
                                                                                                     acATCTTTGAAGTGTATTTTTATCTTCCGTATTATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCt                                   <  2:3230415/139‑1 (MQ=255)
                                                                                                       aTCTTTGACGTGGATGTTCAGCTCCCTGATTATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCttt                                 <  1:1348991/140‑1 (MQ=255)
                                                                                                       aTCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCttt                                 >  1:2807936/1‑140 (MQ=255)
                                                                                                        tCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTc                                >  2:1819726/1‑140 (MQ=255)
                                                                                                           ttGAAGGGGTTGTTGATCTTCCGGATGATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATg                             <  2:259429/140‑1 (MQ=255)
                                                                                                            tGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGACAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCTTCCAATTCTCATCTTTCTCTCGTTTTCCTGTCATGt                            >  1:2145344/1‑140 (MQ=255)
                                                                                                              aaGTGTATGTTGATCTTCCGGATTATAACTAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTg                          <  1:834503/140‑1 (MQ=255)
                                                                                                              aaGTGGTTGTTTTTCGTCCGTATTATAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTg                          <  1:108586/140‑1 (MQ=255)
                                                                                                              aaGTGGTTGTTGATCTTCCGGAGGTTAACGAAGCGACTGTGAAAATTACTGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTg                          <  2:2591875/140‑1 (MQ=255)
                                                                                                               attgtaTGTTGATCTTCCGGATGATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGa                         <  2:3729623/135‑1 (MQ=255)
                                                                                                               aGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACCCCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATACTCATCTTTCTCTCGTTTTCCTTTCATGTTgt                         >  1:2062690/1‑139 (MQ=255)
                                                                                                               aGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGa                         >  1:2662287/1‑140 (MQ=255)
                                                                                                                gtgtttGTTGATCTTCCGGATGATAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAg                        <  2:265250/135‑1 (MQ=255)
                                                                                                                gTGGTTGTTGATCTTCGGTATTTTAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAg                        <  2:857759/140‑1 (MQ=255)
                                                                                                                 tGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGGAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAgt                       >  1:4243653/1‑140 (MQ=255)
                                                                                                                 tGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAgt                       >  2:1766414/1‑140 (MQ=255)
                                                                                                                   gATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAgtgt                     >  2:3661608/1‑140 (MQ=255)
                                                                                                                   gATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAgtgt                     >  2:2310404/1‑140 (MQ=255)
                                                                                                                   gATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAgtgt                     >  2:1516106/1‑140 (MQ=255)
                                                                                                                   gATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAgtgt                     >  1:1440666/1‑140 (MQ=255)
                                                                                                                         taacTTCCGTATTAAAAAGCAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCCTCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAAc               <  1:4135175/137‑1 (MQ=255)
                                                                                                                             ttCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGaa           >  2:1790098/1‑140 (MQ=255)
                                                                                                                              tCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAAc          >  2:1189418/1‑140 (MQ=255)
                                                                                                                               ccGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACt         >  1:75591/1‑140 (MQ=255)
                                                                                                                                   attatAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACTCATa     <  1:3191953/137‑1 (MQ=255)
                                                                                                                                   atgatAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGAAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATATTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACTCATa     <  2:714782/140‑1 (MQ=255)
                                                                                                                                    tgatAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACTCATac    >  2:142629/1‑140 (MQ=255)
                                                                                                                                      atAAAGCAGCGACTGTGAAAATTAAGGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGGGTGTAAACTGAACTCATacaa  <  1:640387/140‑1 (MQ=255)
                                                                                                                                      atAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACTCATacaa  >  2:1695689/1‑140 (MQ=255)
                                                                                                                                      aaaaaGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACTCATacaa  <  1:1699096/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
CAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGG‑ACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTGTGTATGAGGACTGACGGGTTCTCATCAAATTCTCATCTTTCTCTCGTTTTCCTTTCATGTTGAGTGTGTAAACTGAACTCATACAA  >  NZ_CP034943/1365977‑1366249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑