Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0750GC26.9% 201.0 / 22.3 118E166Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (46/30);  new base C (0/28);  total (47/71)
Fisher's exact test for biased strand distribution p-value = 2.15e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 126 total aligned reads displayed.

AGCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAG  >  NZ_CP034943/1729937‑1730208
                                                                                                                                          |                                                                                                                                     
 gCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaa                                                                                                                                     >  2:331325/1‑140 (MQ=255)
 gCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaa                                                                                                                                     >  1:3425425/1‑140 (MQ=255)
  cGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaaa                                                                                                                                    >  1:3502649/1‑140 (MQ=255)
   gATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGaaagaaaa                                                                                                                                   >  1:499037/1‑140 (MQ=255)
    aTGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGaaagaaaaa                                                                                                                                  <  2:799731/140‑1 (MQ=255)
            aaTGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaa                                                                                                                          >  1:4149542/1‑140 (MQ=255)
              tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                        >  1:1190692/1‑140 (MQ=255)
               ccctcATATGAAAAATCAATTAAGCAAAGAGAAAGCAAGTACGAAATCCCAAAAGATTCAACCCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaaa                                                                                                                       <  2:3256807/136‑1 (MQ=255)
                ccGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGACAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaaaa                                                                                                                      >  1:3959166/1‑140 (MQ=255)
                ccGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaaaa                                                                                                                      >  1:2574169/1‑140 (MQ=255)
                 cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTACAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGACAAAGAAGATAAAAaag                                                                                                                     >  1:122575/1‑140 (MQ=255)
                 cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                     >  2:3645397/1‑140 (MQ=255)
                 agCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACAATAAAAAAGAAACGAAAGAAAAAGAAGATAATAaag                                                                                                                     >  1:2179191/2‑140 (MQ=255)
                   cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAATAAAAAGAAGATAAAAaagaa                                                                                                                   >  2:2136819/1‑140 (MQ=255)
                   cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                   >  1:3964250/1‑140 (MQ=255)
                   cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                   >  2:2802900/1‑140 (MQ=255)
                   cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCGAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGATGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                   >  1:906767/1‑140 (MQ=255)
                      cTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAgaagaa                                                                                                                >  1:2927850/1‑140 (MQ=255)
                         aaaaaTCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGc                                                                                                             >  2:910262/1‑140 (MQ=255)
                                aaTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTg                                                                                                      >  2:3905920/1‑140 (MQ=255)
                                        aaatttaaaGCATGTACGAAATCCAAAAAGATTAAACGATAAAACACAATAAAAGCAACAGATCTTAAAAACAGGCAGACACTAAAAAAGAAACGAAATAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                              <  2:436397/134‑1 (MQ=255)
                                        aaattcaaaGAAATTACTAAATCCCAAAATATACAACGCTAAAAGAGAAACAAAGCAACTGCTCTGAAAAAGAGCCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                              <  2:1953672/134‑1 (MQ=255)
                                        aaaGTTAAAGCAAGTAAGAAATCCAAAAAGATTCAACGCTAAATGATAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAACAGACCAAAGCGACAGTGATAAACTg                                                                                              <  2:1659547/140‑1 (MQ=255)
                                          aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTCAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGACGAAAGCGACAGTGATAAACTGga                                                                                            >  1:554007/1‑140 (MQ=255)
                                          aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGAAACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGga                                                                                            >  1:1680731/1‑140 (MQ=255)
                                             caaaGAAACTACCACACCCCAAAACATTCAACGAAAAACGAGAACAAAACCAACCGCTCTCAAAAACAGGCAGACACTAAAAAAGAAACGAACGAAAACGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGAt                                                                                         <  2:2557628/139‑1 (MQ=255)
                                                    aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAGACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                  >  2:3536250/1‑140 (MQ=255)
                                                      tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                >  1:814972/1‑140 (MQ=255)
                                                      tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACACCTCTGACAAAGAGGCAGACACTATTAAAGAAACGAAAGGACCAGACGATCAACAAGAAGAAAGCGACCGTGATCCACTGGAGATTAAAGCaag                                                                                >  1:2791313/1‑139 (MQ=255)
                                                      tACGAAATCCCAAAAGATTAAACGATAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACCAAACAAAAAGAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                <  2:1755254/140‑1 (MQ=255)
                                                        cGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                              >  2:1454412/1‑140 (MQ=255)
                                                         taaaTCCAAAAATATTAAACGATAAAAGATAATAAAATCAAAAGATCTTAAAAAGAGGAATAAAATAAAAAATAAACCAAACAAAAACAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                             <  2:2158856/139‑1 (MQ=255)
                                                           aaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACAGGCa                                                                           >  2:1174777/1‑140 (MQ=255)
                                                             tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAACAAGAGGCAGACACTAAAAAAGACACGAAAGAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                         >  1:3242228/1‑140 (MQ=255)
                                                               ccAAAATATTCAACTCTAAAAGATAATCAAAGAAACACCAAAGAAAAAGAGGCATACACTAAAAAACAAACTAAAGAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                       <  2:2114186/140‑1 (MQ=255)
                                                                 aaaaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAACAAGAGGCAGACACTAAAAAAGAAACGAAAGACAACGAAGATAAAAAAGTAGAAAGCGACAGTGATAAACTGGAGATTAAAGCCACTGGCACTGAAg                                                                     >  1:429091/1‑140 (MQ=255)
                                                                   aataTTAAACGCTAAAAGTCAAAAAAACCAACACATCTCAAAAAGAGGCAGACAATAAAAAATAAACGAAACAAAAATAAGAAAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                   <  2:906767/137‑1 (MQ=255)
                                                                   aaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                   >  1:2915228/1‑140 (MQ=255)
                                                                   aaGATTCAACGATAAAAGAGAATCAAAGCAACAGCTCTTAAAAAGAGCCAGACACTAAAAAAGAAACGAAACAAAAACACGCTAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                   <  2:707226/140‑1 (MQ=255)
                                                                        tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGCACGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGGGCTTAAAGCCCCTGGCACTGAAGGGTCAtt                                                              >  2:3710804/1‑140 (MQ=255)
                                                                        tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACGGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                              >  1:4247822/1‑140 (MQ=255)
                                                                           ccgatAACAGACAATCAAAGCAACCGCTCTCAAAAAGAGGCACACACTAACAAACAAACGAAACAAAAACAAGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  1:4029775/136‑1 (MQ=255)
                                                                           ccGCTAAAAGATATTCAAAGCACAAGATCTGAAAAAGAGGCACACACTAAAAAATAAACCAACCAAAAAGAAGATAAAACAGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  1:3837796/139‑1 (MQ=255)
                                                                           actcTAAATTAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACAATAAAAAACAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  2:188588/137‑1 (MQ=255)
                                                                           actatAAAACACAATAAAAGCAACAGCTCTTAAAAACAGGAATAAAATAAAAAACAAACGAAACAAAAACAATATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  1:210171/136‑1 (MQ=255)
                                                                           acccaaaaaGATAAAAAAACAAACAGCTCTGAAAAAGAGCCACACACTAAAAAACAAACCAAAAAAAAACAAAATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  1:675083/135‑1 (MQ=255)
                                                                           aaccTAAAACAGAATCAAACCAACAGCTCTTAAAAAGAGTCAGACCCTAAAAAACAAACGAAACAAAAACACCATAAAAAAGAAGAAAGCGACAGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  2:3497161/137‑1 (MQ=255)
                                                                           aCGCTAACACACAATCAAAGAAACACCTAAGAAAAAGAGGCACACAATAAAAAACAACCGAAAGAAAAAGACCATAAAAAAGACCCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  2:3994515/140‑1 (MQ=255)
                                                                           aCGCTAAAATACAATCAAAGCAACAGATATTAAAAATAGGCAGAAAATAAAAAATAAACGACACAAAAACAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGAATAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  2:3994461/140‑1 (MQ=255)
                                                                           aCGCTAAAACAGAATCAAACCAACACCTATGAAAAAGAGTCATAAAATAAAAAAGAAACGAAACAAAAACACGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  2:3682937/140‑1 (MQ=255)
                                                                           aCGATAAAATACAAAAAAAGCAACAGCTCTCAAAAAGAGCCACACACAAAAAAACAAACCAAACAAAAACAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  1:2871373/140‑1 (MQ=255)
                                                                           aCGATAAAAGACAATCAAAGCAACAGCTCTGAAAACGAGGCAGCAACTAAAAAACAAACGAAACAAAAAGACCATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  2:410341/140‑1 (MQ=255)
                                                                           aCGATAAAAGACAATAAACCCACCCCCTCACAAAAACAGGCACACACAAAAAAAGACACCAAACAAAAACAACAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                           <  1:3536250/140‑1 (MQ=255)
                                                                                   agagAATCACACCAACAGCTCTCAAAAAGAGGCACACAATAAAAAACAAACCAAACAAAAACAAGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                   <  2:3234314/140‑1 (MQ=255)
                                                                                   agagAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                   <  2:2927850/140‑1 (MQ=255)
                                                                                   agagAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                   <  2:814972/140‑1 (MQ=255)
                                                                                   agagAATCAAAGAAACAGCTCTTAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                   <  2:3425425/140‑1 (MQ=255)
                                                                                     agaATCAAAGCAACAGCTCTGAAAAAGAGGCAGACAATAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                 <  2:1081549/140‑1 (MQ=255)
                                                                                     agaATCAAAGCAACAGCTATTAAAAAGAGCCACACACAAAAAAATAAACGAAAGAAAAACAACATAAAAAACACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                 <  2:1510687/140‑1 (MQ=255)
                                                                                     agaATCAAACCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                 <  2:1509256/140‑1 (MQ=255)
                                                                                      gaATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAg                                                >  1:608337/1‑140 (MQ=255)
                                                                                      gaATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAg                                                >  1:579411/1‑140 (MQ=255)
                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                              >  1:4252001/1‑93 (MQ=255)
                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                              <  2:4252001/93‑1 (MQ=255)
                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                               >  2:4117959/1‑140 (MQ=255)
                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                               >  1:3234314/1‑140 (MQ=255)
                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                               >  2:2081454/1‑140 (MQ=255)
                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACT‑AAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAAATGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTAATAAgtt                                              >  2:2270745/1‑139 (MQ=255)
                                                                                         tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                             >  1:228690/1‑140 (MQ=255)
                                                                                          aaaaCCAACCGCTCTCAAAAACAGGCCCACAATAAAAAACAAACCAAAAAAAAACAACAAAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                            <  1:331325/139‑1 (MQ=255)
                                                                                          aaaaCCAACAGCTCTCAAAAACACCAACACAATAAAAAACAAACCAAAAAAAAACACCATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                            <  1:3468461/139‑1 (MQ=255)
                                                                                             aGCAACAGCTCTGAAAAAGAGGCAGACACGAAAAAAGAAACGAAAGAAAAAGAAGATCAAAAAGAAGAAAGCGACAGTGATAAACTGGCGATTAAAGCCACTGGCACTGAAGGGTCATTAACGACTTCTGACGTGTCCgg                                         >  2:3656942/1‑140 (MQ=255)
                                                                                               caacccctctCAAAAACACCCACACAAAAAAAAACAACCCAAACAAAAATACCAGAAAAAACCAGACAGCGACAGTGATAAACTGGAGATAAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGgcg                                       <  1:1637121/134‑1 (MQ=255)
                                                                                                  cacatctGAAAAAGAGGCACACAAAAAAAAACAAACGAAACAAAAACAAGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTg                                    <  1:1924607/136‑1 (MQ=255)
                                                                                                  cAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACGTATGAAGTGTCCGGCGCTg                                    >  2:2532831/1‑140 (MQ=255)
                                                                                                       ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGgcgcctgtctc                               >  1:3762148/1‑133 (MQ=255)
                                                                                                             cagagCCAGACTCTAAAAAAGAAACGAAAGAACAAGACGATACAACAGAAGAAAGCGACAGTGATAAACTGGACATTAAAGCAACTGGCTCTGTAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAg                         <  2:3152641/139‑1 (MQ=255)
                                                                                                              agagGCAGACACTAAAAAAGAAACGAAAGAAAAACCACATACAAAAGACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                        <  2:3124878/140‑1 (MQ=255)
                                                                                                                       cacaAAAAAATAAAGCAAATAAAAATAAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:1680731/140‑1 (MQ=255)
                                                                                                                       cacTAAAAAATAAACTAAAGAAAACTAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  1:857794/140‑1 (MQ=255)
                                                                                                                       cacTAAAAAATAAACTAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:554007/140‑1 (MQ=255)
                                                                                                                       cacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTGATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:3959166/140‑1 (MQ=255)
                                                                                                                       cacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:2574169/140‑1 (MQ=255)
                                                                                                                       caatAAAAAATAAACCAAACAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:2037246/137‑1 (MQ=255)
                                                                                                                       caatAAAAAAGAAACGAAAGAAAAACATGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  1:910262/137‑1 (MQ=255)
                                                                                                                       caatAAAAAACAACCGAAATAAACTGAAGATAAAAAACAACAACGCGACAGTGATAACCTGGAGATTAAAGCTACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  1:3741967/137‑1 (MQ=255)
                                                                                                                       caatAAAAAACAAACCAAAGAAAACCAAGAAAAAAACCAAGAACGCGACCGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  1:1605080/137‑1 (MQ=255)
                                                                                                                       acaaaaaaaaTAAAATAAACAAAAAGAAGAAAACAAACACCCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  1:2219204/136‑1 (MQ=255)
                                                                                                                       aacTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGATGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:3577061/139‑1 (MQ=255)
                                                                                                                       aaatAAAAACGAAACGAAACAAAAACAACCAAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  1:3742004/137‑1 (MQ=255)
                                                                                                                       aaatAAAAAATAAACTAAATAAAAACAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  1:1299393/137‑1 (MQ=255)
                                                                                                                       aaatAAAAAAGAAACGAAATAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:2887352/137‑1 (MQ=255)
                                                                                                                       aaatAAAAAAGAAACGAAAGAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa               <  2:770854/137‑1 (MQ=255)
                                                                                                                          tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg            >  2:1377727/1‑140 (MQ=255)
                                                                                                                                 gAAACGACAGAAAAAGATGATAAAAAACACTAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACGGAAGGCATCAGa     <  2:1131908/140‑1 (MQ=255)
                                                                                                                                 caaaCTAAAAAAAAATAACATAAAAAATAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa     <  1:2270745/139‑1 (MQ=255)
                                                                                                                                 aaacacaaaaaaaaacaaaaaaaaaaacaaCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa     <  1:1454412/134‑1 (MQ=255)
                                                                                                                                  aaaCGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGCAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc    >  1:2827777/1‑140 (MQ=255)
                                                                                                                                   aaCTAAACAAAAAGAATATAAAAAACAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACa   <  2:1756290/140‑1 (MQ=255)
                                                                                                                                    aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg  >  1:2253048/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                     
AGCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAG  >  NZ_CP034943/1729937‑1730208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑