Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0990AC27.2% 159.5 / 11.6 103S174R (AGC→CGC) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base A (31/44);  new base C (0/28);  total (31/72)
Fisher's exact test for biased strand distribution p-value = 6.77e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 103 total aligned reads displayed.

AAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCG  >  NZ_CP034943/1729962‑1730228
                                                                                                                                         |                                                                                                                                 
  aaaTCATTTCCGCGAAGTGAAAGAAAGTACTAAATCCAAAAAGATTCAACGATAAAACAGAATCAAAGAAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAAAGAAAAAGAAGATAAAAAAGAACAAAGCGa                                                                                                                               <  2:808450/140‑1 (MQ=255)
  aaaTCAATTACGCAAAGTGAAAGCAAGTACGACATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGAAACTAAAAAAGAAACGAAATAAAAAGACCATAAAAAACAAGAAAGCGa                                                                                                                               <  2:3154367/140‑1 (MQ=255)
     tCAATTACGAAAAGTGAACCAAATTACCAAATCCCAAAATATTAAACGCAAAAAAATAATCAAAGAAACACAACTGAAAAAGAGCCATACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAg                                                                                                                            <  2:2818788/140‑1 (MQ=255)
       aaTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTg                                                                                                                          >  2:3905920/1‑140 (MQ=255)
              aaaatttaaaGCAATTACTAAATCACAAATGATTCAACTATAAAACAGAATCAAAGAAACAGCTCTTAAAATGAGGCAGACACTAAAAAAGAAACCAAAGAAAAATAAGATAAAAAAGAAGAAAGCGACAGTGATAAACt                                                                                                                   <  2:2082136/133‑1 (MQ=255)
               aaatttaaaGCATGTACGAAATCCAAAAAGATTAAACGATAAAACACAATAAAAGCAACAGATCTTAAAAACAGGCAGACACTAAAAAAGAAACGAAATAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                                  <  2:436397/134‑1 (MQ=255)
               aaattcaaaGAAATTACTAAATCCCAAAATATACAACGCTAAAAGAGAAACAAAGCAACTGCTCTGAAAAAGAGCCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                                  <  2:1953672/134‑1 (MQ=255)
               aaaGTTAAAGCAAGTAAGAAATCCAAAAAGATTCAACGCTAAATGATAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAACAGACCAAAGCGACAGTGATAAACTg                                                                                                                  <  2:1659547/140‑1 (MQ=255)
               aaaGTTAAAGAAAGTACGAAATCCCAAAAGATTCAACGCTAAAACAGAATCAAAGCAACAGCTCTGAAAAAGAGGCACACACTAAAAAATAAACGAAAGAAAAACAAGATAAAAAAGAACAAAGCGACAGTGATAAACTg                                                                                                                  <  2:1819367/140‑1 (MQ=255)
                 aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTCAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGACGAAAGCGACAGTGATAAACTGga                                                                                                                >  1:554007/1‑140 (MQ=255)
                 aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGAAACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGga                                                                                                                >  1:1680731/1‑140 (MQ=255)
                    caaaGAAACTACCACACCCCAAAACATTCAACGAAAAACGAGAACAAAACCAACCGCTCTCAAAAACAGGCAGACACTAAAAAAGAAACGAACGAAAACGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGAt                                                                                                             <  2:2557628/139‑1 (MQ=255)
                        gCAATTACGAAATCCCAAAAGATTCAACGCTAAAATAGAATCAAAGCAACAGCTCTGAAAAAGAGGAAGACAATAAAAAATAAACGAAACAAAAAGAAGATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTaaa                                                                                                         <  2:3593963/140‑1 (MQ=255)
                           aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAGACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                      >  2:3536250/1‑140 (MQ=255)
                             tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                    >  1:814972/1‑140 (MQ=255)
                             tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                    >  2:3878128/1‑140 (MQ=255)
                             tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACACCTCTGACAAAGAGGCAGACACTATTAAAGAAACGAAAGGACCAGACGATCAACAAGAAGAAAGCGACCGTGATCCACTGGAGATTAAAGCaag                                                                                                    >  1:2791313/1‑139 (MQ=255)
                             tACGAAATCCCAAAAGATTAAACGATAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACCAAACAAAAAGAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                    <  2:1755254/140‑1 (MQ=255)
                               cGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                                  >  2:1454412/1‑140 (MQ=255)
                                taaaTCCAAAAATATTAAACGATAAAAGATAATAAAATCAAAAGATCTTAAAAAGAGGAATAAAATAAAAAATAAACCAAACAAAAACAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                                 <  2:2158856/139‑1 (MQ=255)
                                  aaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACAGGCa                                                                                               >  2:1174777/1‑140 (MQ=255)
                                    tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAACAAGAGGCAGACACTAAAAAAGACACGAAAGAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                             >  1:3242228/1‑140 (MQ=255)
                                      ccAAAATATTCAACTCTAAAAGATAATCAAAGAAACACCAAAGAAAAAGAGGCATACACTAAAAAACAAACTAAAGAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                           <  2:2114186/140‑1 (MQ=255)
                                        aaaaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAACAAGAGGCAGACACTAAAAAAGAAACGAAAGACAACGAAGATAAAAAAGTAGAAAGCGACAGTGATAAACTGGAGATTAAAGCCACTGGCACTGAAg                                                                                         >  1:429091/1‑140 (MQ=255)
                                          aataTTAAACGCTAAAAGTCAAAAAAACCAACACATCTCAAAAAGAGGCAGACAATAAAAAATAAACGAAACAAAAATAAGAAAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                       <  2:906767/137‑1 (MQ=255)
                                          aaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                       >  1:2915228/1‑140 (MQ=255)
                                          aaGATTCAACGATAAAAGAGAATCAAAGCAACAGCTCTTAAAAAGAGCCAGACACTAAAAAAGAAACGAAACAAAAACACGCTAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                       <  2:707226/140‑1 (MQ=255)
                                               tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGCACGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGGGCTTAAAGCCCCTGGCACTGAAGGGTCAtt                                                                                  >  2:3710804/1‑140 (MQ=255)
                                               tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                                  >  1:3450933/1‑140 (MQ=255)
                                                  ccgatAACAGACAATCAAAGCAACCGCTCTCAAAAAGAGGCACACACTAACAAACAAACGAAACAAAAACAAGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:4029775/136‑1 (MQ=255)
                                                  ccGCTAAAAGATATTCAAAGCACAAGATCTGAAAAAGAGGCACACACTAAAAAATAAACCAACCAAAAAGAAGATAAAACAGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:3837796/139‑1 (MQ=255)
                                                  actcTAAATTAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACAATAAAAAACAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  2:188588/137‑1 (MQ=255)
                                                  actatAAAACACAATAAAAGCAACAGCTCTTAAAAACAGGAATAAAATAAAAAACAAACGAAACAAAAACAATATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:210171/136‑1 (MQ=255)
                                                  acgaaaaaacaaaaacaAACAAACACATCTGAAAAAGCGCCAGAAACAAAAAACCAAACCAAACAAAAACAACATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:931126/135‑1 (MQ=255)
                                                  acgaaaaaaTACAATAAAACAAACATCTCTCAAAAAGAGCCACACAATAAAAAACAAACCAAACAAAAACAACATAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:517079/135‑1 (MQ=255)
                                                  acccaaaaaGATAAAAAAACAAACAGCTCTGAAAAAGAGCCACACACTAAAAAACAAACCAAAAAAAAACAAAATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:675083/135‑1 (MQ=255)
                                                  aaccTAAAACAGAATCAAACCAACAGCTCTTAAAAAGAGTCAGACCCTAAAAAACAAACGAAACAAAAACACCATAAAAAAGAAGAAAGCGACAGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  2:3497161/137‑1 (MQ=255)
                                                  aCGCTAACACACAATCAAAGAAACACCTAAGAAAAAGAGGCACACAATAAAAAACAACCGAAAGAAAAAGACCATAAAAAAGACCCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  2:3994515/140‑1 (MQ=255)
                                                  aCGCTAAAATACAATCAAAGCAACAGATATTAAAAATAGGCAGAAAATAAAAAATAAACGACACAAAAACAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGAATAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  2:3994461/140‑1 (MQ=255)
                                                  aCGCTAAAACAGAATCAAACCAACACCTATGAAAAAGAGTCATAAAATAAAAAAGAAACGAAACAAAAACACGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  2:3682937/140‑1 (MQ=255)
                                                  aCGATAAAATACAAAAAAAGCAACAGCTCTCAAAAAGAGCCACACACAAAAAAACAAACCAAACAAAAACAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:2871373/140‑1 (MQ=255)
                                                  aCGATAAAAGAGAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAAAGAAAAAGAAGATAAAAAAGAATAAAGCGACAGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  2:3339061/140‑1 (MQ=255)
                                                  aCGATAAAAGACAATCAAAGCAACAGCTCTGAAAACGAGGCAGCAACTAAAAAACAAACGAAACAAAAAGACCATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  2:410341/140‑1 (MQ=255)
                                                  aCGATAAAAGACAATAAACCCACCCCCTCACAAAAACAGGCACACACAAAAAAAGACACCAAACAAAAACAACAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                               <  1:3536250/140‑1 (MQ=255)
                                                    gCTAAAATACAAACAAACCAACCCCTCTCAAAAAGAGGCAGACAATAAAAAACAAACCAAAAAAAAAGAAGATAAAAAACAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGa                                                                             <  1:3878128/140‑1 (MQ=255)
                                                          agagAATCACACCAACAGCTCTCAAAAAGAGGCACACAATAAAAAACAAACCAAACAAAAACAAGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                       <  2:3234314/140‑1 (MQ=255)
                                                          agagAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                       <  2:2927850/140‑1 (MQ=255)
                                                          agagAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                       <  2:814972/140‑1 (MQ=255)
                                                          agagAATCAAAGAAACAGCTCTTAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                       <  2:3425425/140‑1 (MQ=255)
                                                           cacaaTCAAAGCAAAACCTCTGAAAAACTTGCAGACAATAAAAAACAAACTAAAGAAAACGAAGATCAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGa                                                                      <  1:4201077/137‑1 (MQ=255)
                                                            agaATCAAAGCAACAGCTCTGAAAAAGAGGCAGACAATAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                     <  2:1081549/140‑1 (MQ=255)
                                                            agaATCAAAGCAACAGCTATTAAAAAGAGCCACACACAAAAAAATAAACGAAAGAAAAACAACATAAAAAACACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                     <  2:1510687/140‑1 (MQ=255)
                                                            agaATCAAACCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                     <  2:1509256/140‑1 (MQ=255)
                                                             gaATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAg                                                                    >  1:579411/1‑140 (MQ=255)
                                                             gaATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAg                                                                    >  1:608337/1‑140 (MQ=255)
                                                              aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                                  <  2:4252001/93‑1 (MQ=255)
                                                              aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                                  >  1:4252001/1‑93 (MQ=255)
                                                              aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                   >  2:4117959/1‑140 (MQ=255)
                                                              aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                   >  2:2081454/1‑140 (MQ=255)
                                                              aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                   >  1:3234314/1‑140 (MQ=255)
                                                              aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACT‑AAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAAATGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTAATAAgtt                                                                  >  2:2270745/1‑139 (MQ=255)
                                                                tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                 >  1:228690/1‑140 (MQ=255)
                                                                 aaaaCCAACCGCTCTCAAAAACAGGCCCACAATAAAAAACAAACCAAAAAAAAACAACAAAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                <  1:331325/139‑1 (MQ=255)
                                                                 aaaaCCAACAGCTCTCAAAAACACCAACACAATAAAAAACAAACCAAAAAAAAACACCATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                <  1:3468461/139‑1 (MQ=255)
                                                                    aGCAACAGCTCTGAAAAAGAGGCAGACACGAAAAAAGAAACGAAAGAAAAAGAAGATCAAAAAGAAGAAAGCGACAGTGATAAACTGGCGATTAAAGCCACTGGCACTGAAGGGTCATTAACGACTTCTGACGTGTCCgg                                                             >  2:3656942/1‑140 (MQ=255)
                                                                      caacccctctCAAAAACACCCACACAAAAAAAAACAACCCAAACAAAAATACCAGAAAAAACCAGACAGCGACAGTGATAAACTGGAGATAAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGgcg                                                           <  1:1637121/134‑1 (MQ=255)
                                                                         cacatctGAAAAAGAGGCACACAAAAAAAAACAAACGAAACAAAAACAAGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTg                                                        <  1:1924607/136‑1 (MQ=255)
                                                                         cAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACGTATGAAGTGTCCGGCGCTg                                                        >  2:2532831/1‑140 (MQ=255)
                                                                              ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGgcgcctgtctc                                                   >  1:3762148/1‑133 (MQ=255)
                                                                                    cagagCCAGACTCTAAAAAAGAAACGAAAGAACAAGACGATACAACAGAAGAAAGCGACAGTGATAAACTGGACATTAAAGCAACTGGCTCTGTAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAg                                             <  2:3152641/139‑1 (MQ=255)
                                                                                     agagGCAGACACTAAAAAAGAAACGAAAGAAAAACCACATACAAAAGACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                            <  2:3124878/140‑1 (MQ=255)
                                                                                      gagGAATACAATAAAAAAGAAACTAAATAAAAATAATATAAAAAATAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                           <  2:470724/140‑1 (MQ=255)
                                                                                          catacaaTAAAAAACAAACCAAAAAAAAATAATATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGaa                                       <  1:1544249/137‑1 (MQ=255)
                                                                                           aGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAAc                                      >  1:3177476/1‑140 (MQ=255)
                                                                                              cacaAAAAAATAAAGCAAATAAAAATAAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  2:1680731/140‑1 (MQ=255)
                                                                                              cacaAAAAAACAAACCAAACAAAAATACCATAAAAAAGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:3645397/140‑1 (MQ=255)
                                                                                              cacTAAAAAATAAACTAAAGAAAACTAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:857794/140‑1 (MQ=255)
                                                                                              cacTAAAAAATAAACTAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  2:554007/140‑1 (MQ=255)
                                                                                              cacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTGATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  2:3959166/140‑1 (MQ=255)
                                                                                              caatAAAAAATAAACCAAACAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  2:2037246/137‑1 (MQ=255)
                                                                                              caatAAAAAAGAAACGAAAGAAAAACATGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:910262/137‑1 (MQ=255)
                                                                                              caatAAAAAACAACCGAAATAAACTGAAGATAAAAAACAACAACGCGACAGTGATAACCTGGAGATTAAAGCTACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:3741967/137‑1 (MQ=255)
                                                                                              caatAAAAAACAAACCAAAGAAAACCAAGAAAAAAACCAAGAACGCGACCGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:1605080/137‑1 (MQ=255)
                                                                                              caacaaaacaGAAACGAAACAAAAACAACATAAAACACAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:2802900/136‑1 (MQ=255)
                                                                                              acaaaaaaaaTAAAATAAACAAAAAGAAGAAAACAAACACCCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:2219204/136‑1 (MQ=255)
                                                                                              aacTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGATGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  2:3577061/139‑1 (MQ=255)
                                                                                              aaatAAAAACGAAACGAAACAAAAACAACCAAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:3742004/137‑1 (MQ=255)
                                                                                              aaatAAAAAATAAACTAAATAAAAACAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  1:1299393/137‑1 (MQ=255)
                                                                                              aaatAAAAAAGAAACGAAATAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  2:2887352/137‑1 (MQ=255)
                                                                                              aaatAAAAAAGAAACGAAAGAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                   <  2:770854/137‑1 (MQ=255)
                                                                                                 tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg                                >  2:1377727/1‑140 (MQ=255)
                                                                                                   aaaaaCAAACCAAACAAAAAGACGATAAAAAAGAAGACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                              <  1:2268742/140‑1 (MQ=255)
                                                                                                        gAAACGACAGAAAAAGATGATAAAAAACACTAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACGGAAGGCATCAGa                         <  2:1131908/140‑1 (MQ=255)
                                                                                                        caaaCTAAAAAAAAATAACATAAAAAATAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                         <  1:2270745/139‑1 (MQ=255)
                                                                                                        aaacacaaaaaaaaacaaaaaaaaaaacaaCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                         <  1:1454412/134‑1 (MQ=255)
                                                                                                         aaaCGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGCAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc                        >  1:2827777/1‑140 (MQ=255)
                                                                                                          aaCTAAACAAAAAGAATATAAAAAACAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACa                       <  2:1756290/140‑1 (MQ=255)
                                                                                                           aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                      >  1:2253048/1‑140 (MQ=255)
                                                                                                                   aaaccaagaTAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa              <  2:2915228/135‑1 (MQ=255)
                                                                                                                               aaaaGACGCACGCGACAGTGATACACTGGAGATTAAAGCCACGGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAcgcg  <  1:2108030/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                 
AAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCG  >  NZ_CP034943/1729962‑1730228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑