Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,408,7270CA25.0% 246.7 / 27.0 152A327S (GCG→TCG) spoIIIAEstage III sporulation protein AE
Reads supporting (aligned to +/- strand):  ref base C (50/64);  new base A (0/38);  total (50/102)
Fisher's exact test for biased strand distribution p-value = 1.68e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 156 total aligned reads displayed.

GCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAG  >  NZ_CP034943/2408588‑2408861
                                                                                                                                            |                                                                                                                                      
gCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGc                                                                                                                                        >  2:897577/1‑140 (MQ=255)
gCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCGTCTAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGc                                                                                                                                        >  2:3413431/1‑140 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                    <  1:2672016/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                    <  1:2580749/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                    >  1:3332492/1‑140 (MQ=255)
      aaaaTATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACa                                                                                                                                  <  2:1538409/140‑1 (MQ=255)
        aaTATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGa                                                                                                                                <  1:231409/140‑1 (MQ=255)
         atatAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGAt                                                                                                                               >  1:3577364/1‑140 (MQ=255)
           ataAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCa                                                                                                                             >  2:3252391/1‑140 (MQ=255)
            taAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGACAGCACTTTGATTGCCGGAAAAGCCGCACTACCGATCaa                                                                                                                            >  1:1865657/1‑140 (MQ=255)
                   cGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCg                                                                                                                     >  2:1024911/1‑140 (MQ=255)
                   cGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCCGCTGAAGAATCGCGCCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTGGATTGCCGGAAAAGCCGCCAAACAGAGCAAAAAAGCg                                                                                                                     >  2:3891681/1‑140 (MQ=255)
                    gCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACATATCAAAATAGCGa                                                                                                                    >  1:2214168/1‑140 (MQ=255)
                    gCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGa                                                                                                                    >  1:1228132/1‑140 (MQ=255)
                    gCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGa                                                                                                                    >  1:1073253/1‑140 (MQ=255)
                         tGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACATATCAAAAGCGCGACTCCg                                                                                                               <  2:212288/140‑1 (MQ=255)
                           cTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCACGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGTAACGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCgag                                                                                                             <  2:4243989/140‑1 (MQ=255)
                              atgcggTCGAGGCAGTTACTGACGGGCCCGCCTCCACGCGGCTGAAGAAGCGCGGCTGCCAGTTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                          <  1:3400612/135‑1 (MQ=255)
                              atgaCGTCGAGGCAGGTACTGACGGCCCCCCCGCCACGCGGCTGAAGAAGCGCGGCTGCCAGCTTATAAATAAACGCG‑AGGGAAAGCACTTTGTTTGCCGGAAAAGCCGCAATACATATCAAAATAGCGACTCCGAGAAt                                                                                                          <  2:1182638/140‑1 (MQ=255)
                                gaCGTCGAGCCAGGTAATGACGGGCCCGCCTCCACGCGGCGGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑CGTGAACGCACTTTGATTCCCGGAAAAGCCGAAATACAGATCAAAATCGCGACTCCGAGAATcc                                                                                                        <  2:3641662/140‑1 (MQ=255)
                                        ggaaGGAAATGAGGGGCCCGCCCCCACGCGGCTTAAGATTCGCGGATGCACGTTAATAAATACAAGCT‑AGGCAAAGCACTTTTATGGCCGGAAAAGCCGAAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTa                                                                                                <  2:826701/137‑1 (MQ=255)
                                        ggCTGGTATTGACGGGCCCGCCTCCACGCGGCTGAAGATTCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGTAAAGCACTTTGATTGCCGTAAAAGCCGAAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTa                                                                                                <  2:3332492/140‑1 (MQ=255)
                                        ggCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTa                                                                                                >  2:3564855/1‑140 (MQ=255)
                                             gTAATTACGGGCCAGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGTGACAGCACTTTGATTGCAGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           >  2:3856900/1‑140 (MQ=255)
                                             gTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           >  2:685427/1‑140 (MQ=255)
                                              taaggccGGGCCCGCCTCCAAGGGGCTGAACACTCGCGGCTGCCTGATTATAAATAAACGCG‑AGGGAAAGCACTTTGATTGCCGGAAACGCCGCAAAACAAATCAAAAAAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                          <  1:646059/134‑1 (MQ=255)
                                              tAATGACGGGCCCGCCTCCACGCGGCTGAAGACGCGCGCCTGCCAGAAGATCACTAAACGCG‑AGGGAAACCACGTTGATTGCCGGAAACGCCGCAATACCCATCAAAATCGCCACTCCGAGAATCCCTACTGTATTTTTc                                                                                          <  2:1861919/140‑1 (MQ=255)
                                              tAATGACGGCCCCGCCTCCACGCGGCTCAACAAGCGCGGCTGCAAGATTATAAATAACCGCG‑AGGGAAACCACTTTTATTGCCGCAAACGCCCCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                          <  1:715996/140‑1 (MQ=255)
                                              tAAGGACGGGCCCCACACAAAGCGTCTTAATAATCGGTGATGCAAGGTTATAAATAAAAGCG‑AGGGAAAGCACTTTAATTGCCGGAAAAGCCGCAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                          <  2:2480520/140‑1 (MQ=255)
                                               aaTGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTca                                                                                         >  2:3176494/1‑140 (MQ=255)
                                                atgcggggCCCGCCTCCAAGCGGCTGAATAAGCGCGGAGGCCACATTATAAATAAACCCG‑TGGGAAAGACCTTTGTTTGCCGCAAAAGCCGCAATAAAGAAAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                        <  1:3581968/135‑1 (MQ=255)
                                                atcccGGCCCCGCCCCCACGCGGCGCAAGACGCGCGGCTGCCCGCTTATAACTAACCGCG‑CGGCAACGCAGTTTGATGGCCGGAAAAGCCCCAAGACACAAAACAAGAGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                        <  1:1876863/136‑1 (MQ=255)
                                                aTGACGGGCCCGCCTCCACGCGGCTGAAGAATCGGGGCTGCCAGCTTATAAATAAAACCG‑AGGCAAACCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                        <  2:1960645/140‑1 (MQ=255)
                                                   aCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcagcag                                                                                     >  2:2151240/1‑140 (MQ=255)
                                                   aCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGAG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcagcag                                                                                     >  1:82966/1‑140 (MQ=255)
                                                     gggCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACAGTATTTTTCAGCAGTa                                                                                   >  2:933432/1‑140 (MQ=255)
                                                      ggCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTaa                                                                                  >  1:2938034/1‑140 (MQ=255)
                                                       gCCCGCCTCCAATCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  1:3161659/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  1:1976823/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  1:1828712/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  2:3296245/1‑140 (MQ=255)
                                                        ccccccTCCAAGCGGCTTAAGAATCGCGGCTCCCAGGTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                <  2:489238/140‑1 (MQ=255)
                                                        cccGCCTCCAAGCGGCTCAACACTCGCGGGGGCCAGCTTAGAAATAAACGCG‑AGGGAACGCACTTTTATTGCCGGAAACGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                <  2:3519402/140‑1 (MQ=255)
                                                        cccGCCTCAAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGTAAAGCACTTTGTTTGCCGGAAAAGCCGAAATACAGAAAAAAAACGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                <  1:3039756/140‑1 (MQ=255)
                                                        cccGCCGCCACGCGGCTGAAGCACCGCGGCTGCCAGCTTATAAATAAACGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCATTCAAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                <  2:1486904/140‑1 (MQ=255)
                                                        cccGCCCCCACGCGGGTTAACACTCGCGGCGGCCATCTTATAAAAAAAAGCG‑CGGCAAAGCACTTTGATTCCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                <  2:3504152/140‑1 (MQ=255)
                                                             caccACGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAACGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGACTCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  2:1941569/138‑1 (MQ=255)
                                                             cTCCAAGCGGCTTAATAATCGCGGGTGCCTGCTTATAAAAAAACGCT‑AGGGAAACCACTTTGATGGCCGCAAACGCCGCAAAAAAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  1:3387987/140‑1 (MQ=255)
                                                             cTCCAAGCGGCTTAAGAATCGCGGCTGCCAGCTTATAAATAAACGCG‑AGGTAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAAAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  2:543582/140‑1 (MQ=255)
                                                             cTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  2:2669041/140‑1 (MQ=255)
                                                             atCCAAGCGGCTTATGACCCGGGGGGGCCAGTTTATAAA‑AAAACCGTTGGGAATCCAGTTTGATGGCCTGAAACGCCGAAAAACAGTACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  1:2914880/139‑1 (MQ=255)
                                                               cccaGCGGCTGAAGAATCGCGGCTGCCAGCTAAAAAATAAACGCG‑CGGCAACGCACTTTGATTGCCGTAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                         <  1:3244498/137‑1 (MQ=255)
                                                               caaaGCGGCGGAACAATCGCGGCTGCAAGCTTATAATTAATACCG‑AGTGAAAGCACTTTGTTGGCCGGAAAAGCGGCATTACTGATAAAACTATCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                         <  2:3423497/138‑1 (MQ=255)
                                                                cacgCGGCTGACGAATCGCGGGTGCCAGTTTATAAATAAACGCG‑AGGGAACCAACTTTGTTTGCCGGAAAAGCCGAAATACAGAACAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTg                                                                        <  1:3252391/137‑1 (MQ=255)
                                                                cacgCGGATGAAGAATCGCGGCTCCCAGTTTATAAATAACAGCG‑TGGGAAACCACTTTGATGGCCGTAAAAGCCGAAATACAGACCAAACACGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTg                                                                        <  1:486581/137‑1 (MQ=255)
                                                                  cgCGGCTGAAGAATCGGGGCTGCCAGCTTATAAATAACAGCG‑AGGGAAACCACTTTGTTTGCCGCAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                      <  1:2840415/139‑1 (MQ=255)
                                                                  aGCGGCTTAAGAATCGCGGCTGCACTATTATAAATAACAGCG‑CGGGAAAGAAATTTGTTGGCCGGAAACGCCGAAATACATAACAAAACAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                      <  1:3392549/140‑1 (MQ=255)
                                                                   gCGGCTCAAGAATCGCGGCGGCCAGATTATAAAAAAACGCG‑CGGCAAACCAATTTGATTGCCGAAAAACCCGAAATACACAAAAAAACAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATc                                                                     <  1:58613/140‑1 (MQ=255)
                                                                    cggcgtaagaaTCGCGGCTGCCAGCTTATAAATAAAAGCG‑CGGGAAAGCACTTTGATTGCCGTAAAAGCCCAAATACAGATCAAAACAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATca                                                                    <  1:1201572/134‑1 (MQ=255)
                                                                    cggcggAAGAACCGCGGCGCCCAGCTTAAAAATAAAAGCG‑AGGGAAAGCACTTTGATTCCCGGAAAATCCGAAATACAGAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATca                                                                    <  1:2790737/140‑1 (MQ=255)
                                                                     ggCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTTATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                   <  2:3629293/140‑1 (MQ=255)
                                                                     ggCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                   <  2:4094557/140‑1 (MQ=255)
                                                                     ggCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGCAAACCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                   <  2:1810035/140‑1 (MQ=255)
                                                                     ggCTGAAGAAGCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGAGTGCCGGAAAAGCCGCAATACATATCAAAAGAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                   <  2:260396/140‑1 (MQ=255)
                                                                       cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                 >  2:893437/1‑140 (MQ=255)
                                                                       cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                 >  2:4180657/1‑140 (MQ=255)
                                                                       cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                 >  1:595014/1‑140 (MQ=255)
                                                                       cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                 >  1:883355/1‑140 (MQ=255)
                                                                                cgcgGCTGCCAGATTATAAATAAACGCG‑AGGTAAAGCTCTTTGATTGCGGCAAACGCCGAAAAACAGAAAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                        <  1:3896154/140‑1 (MQ=255)
                                                                                cgcgGCTGCCAGATTATAAAAAAACGCT‑AGGCAAACCACTTTGATTGCCGGAAAAGCCGCAAAACAGAAAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                        <  1:3435645/140‑1 (MQ=255)
                                                                                cgcgGCGGCCAGATTATAAATAAACGCG‑AGGGAACGCACTTTGATTGCCGGAAAAGCCGCAATACAGAACAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                        <  1:2116698/140‑1 (MQ=255)
                                                                                 gcgGCTGCCATCTTATAAATAAACGCG‑AGGTAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgc                                                       <  1:3936660/140‑1 (MQ=255)
                                                                                 gcgGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACATATCAAAATAGCGACTCCGAGAATCCCTCCTGTTTTTTTCAGCAGTCAGGAAGCGCTGATCAGATGATCTGTcgc                                                       >  2:2991505/1‑140 (MQ=255)
                                                                                 gcgGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgc                                                       >  2:3208585/1‑140 (MQ=255)
                                                                                  cgGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgcg                                                      >  2:412685/1‑140 (MQ=255)
                                                                                  cgGCTGCCAGATTATAAATAAACGCG‑AGGCAACCCACTTTGATGGCCGCAAAAGCCCCAATACACAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgcg                                                      <  1:1942434/140‑1 (MQ=255)
                                                                                       gCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGt                                                 >  1:1914238/1‑140 (MQ=255)
                                                                                              tataAATAAACGCG‑AGGTAAAGAACTTTTTTGGCGGTAAACGCCGAATTACACAAAAAAACAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACAtt                                          <  1:1720957/140‑1 (MQ=255)
                                                                                               ataaataaAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                   >  1:791585/1‑114 (MQ=255)
                                                                                               ataaataaAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTc                                         >  2:1382474/1‑140 (MQ=255)
                                                                                               ataaataaAAGCG‑AGGGAAAGCACTTTGATTGCCGCAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                   <  2:791585/114‑1 (MQ=255)
                                                                                                 aaataaaCGCG‑AGGCAAAGAATTTTTATTGCCGTAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCgg                                       <  1:3998809/140‑1 (MQ=255)
                                                                                                 aaataaaAGCG‑AGGGAAACCACTTTGATGGCCGCAAAAGCCGAAATACAGAACAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCgg                                       <  1:2526296/140‑1 (MQ=255)
                                                                                                  aataaaAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGc                                      <  2:1054714/140‑1 (MQ=255)
                                                                                                   ataaaAGCG‑CGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:657614/140‑1 (MQ=255)
                                                                                                   ataaaAGCG‑AGGTAAAGCACTTTGATTGCGTTAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  1:2936162/140‑1 (MQ=255)
                                                                                                   ataaaAGCG‑AGGTAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:2949231/140‑1 (MQ=255)
                                                                                                   ataaaAGCG‑AGGTAAAGAACTTTGATTGCCGGAAAAGCCGAAATACATAAAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  1:3176494/140‑1 (MQ=255)
                                                                                                   ataaaAGCG‑AGGTAAACAACTTTTATTGCCGTAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  1:858046/140‑1 (MQ=255)
                                                                                                   ataaaAGCG‑AGGGAAAGCACTTTGATTGCCGTAAACCCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  1:2906809/140‑1 (MQ=255)
                                                                                                   ataaaAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  1:412685/140‑1 (MQ=255)
                                                                                                   ataaaAGCC‑AGGGAAATCTCTTTCATTGCCGTAAAAGCCGCAATACAGAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:590209/140‑1 (MQ=255)
                                                                                                        cgCG‑AGGAAAAGCAATTTTTTGGGCGAAAATCCCGAAATCGAGTTCAATCGAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                                <  1:3295705/139‑1 (MQ=255)
                                                                                                        accG‑AGGGAAAGAACTTTGATTGCCGTAAAAGCCGCAATACCCATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                                <  1:3462587/138‑1 (MQ=255)
                                                                                                                        tttGATTGCCGGAAAAGCCGAAATACAGAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTc                 <  1:4047263/140‑1 (MQ=255)
                                                                                                                               gCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTtat          >  2:1089993/1‑140 (MQ=255)
                                                                                                                               gCCGGAAAAGCCGAAATACAGAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTtat          <  1:2361415/140‑1 (MQ=255)
                                                                                                                                ccGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGACTCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCTAGTACAGGAATAAAGTTTCCGGTtata         >  1:1554447/1‑140 (MQ=255)
                                                                                                                                       aGCCGCAATAAAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAg  <  2:1554447/140‑1 (MQ=255)
                                                                                                                                       aGCCGCAAGACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAg  <  2:130353/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
GCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCG‑AGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAG  >  NZ_CP034943/2408588‑2408861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑