Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP034943 | 2,981,253 | 0 | C | T | 31.6% | ‑3.8 / 13.1 | 19 | noncoding (1267/1550 nt) | EO946_RS15435 | 16S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base C (2/11); new base T (0/6); total (2/17) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGCA > NZ_CP034943/2981117‑2981386 | tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGCCTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCt < 2:3569348/137‑1 (MQ=0) tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt < 1:446085/137‑1 (MQ=1) tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt < 2:3250754/137‑1 (MQ=1) ggttcAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCGTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCt < 1:4127030/136‑1 (MQ=0) gacggcgGGATGGTGCTGCGCGATTACTAGCGATTCGAGGTTGACGCAGTCGAGTTGCTGAGTGCGATCCGATCTGTGAACAGATTTGTGGGATTGGCTTATCGTCGCGGTTTTGCTGCCCTTTGTTCTGTCCTTTGTAg < 2:3911156/136‑1 (MQ=1) cggggCCTGCTGCTCCGCGATTACTAGCGAGTCCAGCTTCCCGCAGTCGAGTTGCAGACTGCGATCCGAACCGAGAACAGAGTTGTGGGAGTGGCTTAACCTCGCGGTTTCGCTGCCCTGTGTACTGTCCATTGTAGCAc < 2:863711/137‑1 (MQ=1) tcagcgATTACTATCTCTTCCATCTTCACGCAGTCGTGTTGCAGACTGCGATCCGAACTGAGAAGAGATTTGTGGGATTGGCTAAACCTCGCTGTTTCGCTGGACTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAg < 2:3989946/137‑1 (MQ=1) cgcttACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCt < 1:2838386/106‑1 (MQ=1) atgaCTAGCGATGCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAAGAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTCTCCATTGTAGCACGTGTGTAGCCCAGGTCATa < 1:2635541/137‑1 (MQ=1) gagtCCCGCTTCACGCCGTCGAGTGGCAGACTGCGATCCGAACTCAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCatg < 2:2557043/137‑1 (MQ=1) cgaccAGATTTGTGGGAGTGGCTGAACCGCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCCCGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCGGGTTTGTCACCGGCAg < 1:4154553/136‑1 (MQ=1) agtaCAGTTTTGTGGGATTGGCTTAGCCTCGCGGTTTTGCGGCCCTTTGTTGTGTCCATTGTCGAACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCGGGTTTGTCACCGGCAg < 2:1599767/137‑1 (MQ=1) agcaCAGATTTGTGGGATTGGCTTCACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAg < 2:3403915/137‑1 (MQ=1) gaccAGATTTGTGGGATTGGCTTACCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGt < 2:469428/137‑1 (MQ=1) gagtGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCAGCCCCACCGTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGcc < 1:1067873/137‑1 (MQ=0) ttcaCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCCTTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGa < 2:83592/137‑1 (MQ=1) ttcaCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGa > 2:1867828/4‑140 (MQ=1) ttcaCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGa < 2:1305035/137‑1 (MQ=1) cgctttCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAACGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGGTTGTCACCGGCAGTCACCTTAGACTGCCCAACTGACTGCTGGCa > 2:661363/4‑140 (MQ=1) | TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGCA > NZ_CP034943/2981117‑2981386 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |