Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,981,2530CT31.6% ‑3.8 / 13.1 19noncoding (1267/1550 nt)EO946_RS1543516S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (2/11);  new base T (0/6);  total (2/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGCA  >  NZ_CP034943/2981117‑2981386
                                                                                                                                        |                                                                                                                                     
tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGCCTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCt                                                                                                                                    <  2:3569348/137‑1 (MQ=0)
tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt                                                                                                                                    <  1:446085/137‑1 (MQ=1)
tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt                                                                                                                                    <  2:3250754/137‑1 (MQ=1)
ggttcAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCGTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCt                                                                                                                                    <  1:4127030/136‑1 (MQ=0)
                       gacggcgGGATGGTGCTGCGCGATTACTAGCGATTCGAGGTTGACGCAGTCGAGTTGCTGAGTGCGATCCGATCTGTGAACAGATTTGTGGGATTGGCTTATCGTCGCGGTTTTGCTGCCCTTTGTTCTGTCCTTTGTAg                                                                                                             <  2:3911156/136‑1 (MQ=1)
                          cggggCCTGCTGCTCCGCGATTACTAGCGAGTCCAGCTTCCCGCAGTCGAGTTGCAGACTGCGATCCGAACCGAGAACAGAGTTGTGGGAGTGGCTTAACCTCGCGGTTTCGCTGCCCTGTGTACTGTCCATTGTAGCAc                                                                                                          <  2:863711/137‑1 (MQ=1)
                                       tcagcgATTACTATCTCTTCCATCTTCACGCAGTCGTGTTGCAGACTGCGATCCGAACTGAGAAGAGATTTGTGGGATTGGCTAAACCTCGCTGTTTCGCTGGACTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAg                                                                                             <  2:3989946/137‑1 (MQ=1)
                                           cgcttACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCt                                                                                                                        <  1:2838386/106‑1 (MQ=1)
                                             atgaCTAGCGATGCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAAGAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTCTCCATTGTAGCACGTGTGTAGCCCAGGTCATa                                                                                       <  1:2635541/137‑1 (MQ=1)
                                                      gagtCCCGCTTCACGCCGTCGAGTGGCAGACTGCGATCCGAACTCAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCatg                                                                              <  2:2557043/137‑1 (MQ=1)
                                                                                                   cgaccAGATTTGTGGGAGTGGCTGAACCGCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCCCGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCGGGTTTGTCACCGGCAg                                 <  1:4154553/136‑1 (MQ=1)
                                                                                                   agtaCAGTTTTGTGGGATTGGCTTAGCCTCGCGGTTTTGCGGCCCTTTGTTGTGTCCATTGTCGAACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCGGGTTTGTCACCGGCAg                                 <  2:1599767/137‑1 (MQ=1)
                                                                                                   agcaCAGATTTGTGGGATTGGCTTCACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAg                                 <  2:3403915/137‑1 (MQ=1)
                                                                                                    gaccAGATTTGTGGGATTGGCTTACCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGt                                <  2:469428/137‑1 (MQ=1)
                                                                                                                  gagtGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCAGCCCCACCGTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGcc                  <  1:1067873/137‑1 (MQ=0)
                                                                                                                         ttcaCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCCTTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGa           <  2:83592/137‑1 (MQ=1)
                                                                                                                         ttcaCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGa           >  2:1867828/4‑140 (MQ=1)
                                                                                                                         ttcaCCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGa           <  2:1305035/137‑1 (MQ=1)
                                                                                                                                  cgctttCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAACGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGGTTGTCACCGGCAGTCACCTTAGACTGCCCAACTGACTGCTGGCa  >  2:661363/4‑140 (MQ=1)
                                                                                                                                        |                                                                                                                                     
TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGCA  >  NZ_CP034943/2981117‑2981386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: