BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP034943	132,186	A→G	D560G (GAC→GGC)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,186	0	A	G	99.7%	1231.9 / NA	347	D560G (GAC→GGC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (217/127); minor base T (0/1); total (218/129)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322

             cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACtt                                                                                                                          >  2:3501631/1‑140 (MQ=255)

             cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCACTTATCGGCATTAAAGCAAAACtt                                                                                                                          >  2:502480/1‑140 (MQ=255)

                 cttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCg                                                                                                                      <  2:1237183/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:2657559/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  1:1765869/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:2968141/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:3391728/140‑1 (MQ=255)

                   ttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGAt                                                                                                                    <  2:110261/140‑1 (MQ=255)

                   ttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGAt                                                                                                                    <  1:1545307/140‑1 (MQ=255)

                         ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              <  1:117250/140‑1 (MQ=255)

                         ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              <  1:72207/140‑1 (MQ=255)

                           gaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTa                                                                                                            >  2:2209255/1‑140 (MQ=255)

                              aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  2:2972713/140‑1 (MQ=255)

                              aaTGCGATTGTCGGTGGGGTAGTTCCAAGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  2:329229/140‑1 (MQ=255)

                                tGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCatg                                                                                                       >  1:756835/1‑140 (MQ=255)

                                     ttGTCGGTGGGGTAGTTCCACGTTAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAATTGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGtt                                                                                                  <  2:772488/140‑1 (MQ=255)

                                                   gTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAt                                                                                    <  1:3652939/140‑1 (MQ=255)

                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGTCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   >  1:441090/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:3474414/101‑1 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     >  1:3474414/1‑101 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTc                                                                                                               >  1:1421978/1‑107 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1477488/1‑140 (MQ=255)

                                                             aaTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCaaaa                                                                                                                             <  2:1216304/89‑1 (MQ=255)

                                                              aTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAg                                                                         >  1:27948/1‑140 (MQ=255)

                                                                 cATCCCAGCTGTTCAAGCACGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTGTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtg                                                                      <  2:2448094/140‑1 (MQ=255)

                                                                  agcccAGCTGTTCAAGCAGGTCAGGAACATGCACTTCAAAATGGTGTACTGGCAGGCTTCCCGCTTAACCGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGGTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     <  1:4071679/138‑1 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     >  2:3092784/1‑140 (MQ=255)

                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:1124383/140‑1 (MQ=255)

                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:152032/140‑1 (MQ=255)

                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  1:1117208/140‑1 (MQ=255)

                                                                   tCCCAGCTGTTCAACCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTGAAAGTtgct                                                                    <  1:655159/140‑1 (MQ=255)

                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCGTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   <  1:63011/140‑1 (MQ=255)

                                                                       aGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCtt                                                                >  2:2139035/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    >  2:3050986/1‑131 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:3929068/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:2377319/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:4115899/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:3026572/1‑140 (MQ=255)

                                                                              cAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGc                                                         >  2:2275134/1‑140 (MQ=255)

                                                                              cAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGc                                                         >  2:1392961/1‑140 (MQ=255)

                                                                                 gCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                                                      <  1:3719926/140‑1 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:3217886/121‑1 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:1980363/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:2873026/1‑140 (MQ=255)

                                                                                           aaGATGCTATTGAAAATTGTGTACTGGCAGGCTTCCCGCTTATCTGCATTAAATCAAAACTTTTCGATTGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTACAGTTGCTGATTCTATGGCATTGAAAAATGCa                                            <  1:2291228/140‑1 (MQ=255)

                                                                                           aaGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAACTGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa                                            <  2:4141946/140‑1 (MQ=255)

                                                                                           aaGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa                                            <  1:1959509/140‑1 (MQ=255)

                                                                                              aTGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTc                                         >  1:224128/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCAt                                                                                                                                       <  1:1061633/45‑1 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCCTTGAAAAATGCAGTCa                                        >  2:2715328/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:909473/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:3714391/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:2410499/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1013999/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2626991/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2546894/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:491109/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:314644/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1627792/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:750294/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1025114/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGATTCTATGGCATTGAAAAATGCAGTCa                                        >  1:2960166/1‑140 (MQ=255)

                                                                                                gCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg                                       >  2:390845/1‑140 (MQ=255)

                                                                                                gCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg                                       >  1:1652131/1‑140 (MQ=255)

                                                                                                gCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg                                       >  2:2724023/1‑140 (MQ=255)

                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  1:3381101/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTATAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  1:2545812/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:3599957/140‑1 (MQ=255)

                                                                                                         aaTGGTGGACTGGCAGGCGTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTaa                              <  1:3247860/140‑1 (MQ=255)

                                                                                                           tGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAAcc                            <  2:2868275/140‑1 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa                                            <  2:372299/121‑1 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa                                            >  1:372299/1‑121 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                         >  2:1296862/1‑140 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                         >  1:3848178/1‑140 (MQ=255)

                                                                                                               gtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  2:2840492/140‑1 (MQ=255)

                                                                                                               gtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  1:563359/140‑1 (MQ=255)

                                                                                                               gtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  1:2693898/140‑1 (MQ=255)

                                                                                                               gtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  1:1627792/140‑1 (MQ=255)

                                                                                                               ggaCTGGCAGGCTTCCCGCTGATCGCCATGAAAGCAACCCTCTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATCGCACTTAAAGGTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTACCCCAGt                        <  2:1414940/138‑1 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  1:1291859/1‑140 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:615461/1‑140 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtg                       >  2:4180168/1‑139 (MQ=255)

                                                                                                                     gCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTtcttct                  >  2:429749/1‑140 (MQ=255)

                                                                                                                     gCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTtcttct                  >  2:1603338/1‑140 (MQ=255)

                                                                                                                       aGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGTCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTg                >  1:1889862/1‑140 (MQ=255)

                                                                                                                       aGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTg                >  1:2844532/1‑140 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGGATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  2:3856926/1‑140 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  1:2006708/1‑140 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  2:3998381/1‑140 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  2:3238343/1‑140 (MQ=255)

                                                                                                                          cTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc             >  1:96503/1‑140 (MQ=255)

                                                                                                                            tCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGTTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCa           <  2:1858173/140‑1 (MQ=255)

                                                                                                                            tCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCa           <  1:3770183/140‑1 (MQ=255)

                                                                                                                              ccGCTTATCGGCAATAAAGCAAAACAGTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAAt         <  1:2436986/140‑1 (MQ=255)

                                                                                                                                gCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTATAGTTGCTGGTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCa       >  2:2465272/1‑140 (MQ=255)

                                                                                                                                gCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCATGAACCAATCa       >  2:1544221/1‑140 (MQ=255)

                                                                                                                                 cTTATCGTCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  2:3830915/140‑1 (MQ=255)

                                                                                                                                 cTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  1:4140830/140‑1 (MQ=255)

                                                                                                                                 cTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACACAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGCACCAATCAt      <  1:2102149/140‑1 (MQ=255)

                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   <  1:3111269/140‑1 (MQ=255)

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`