Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 828,787 G→A E77K (GAA→AAA)  treC → alpha,alpha‑phosphotrehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943828,7870GA99.6% 864.3 / NA 226E77K (GAA→AAA) treCalpha,alpha‑phosphotrehalase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (101/124);  minor base C (0/1);  total (101/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

GCGATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGA  >  NZ_CP034943/828650‑828923
                                                                                                                                         |                                                                                                                                        
gCGATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTaaa                                                                                                                                        <  1:1130791/140‑1 (MQ=255)
gCGATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTaaa                                                                                                                                        <  1:3828676/140‑1 (MQ=255)
     cTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACgg                                                                                                                                   >  2:3236000/1‑140 (MQ=255)
       taaCGTCATTATTGAAAAGCTTTATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGa                                                                                                                                 <  2:2902547/139‑1 (MQ=255)
       gAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGa                                                                                                                                 <  1:1063519/140‑1 (MQ=255)
       gAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGa                                                                                                                                 <  1:1970867/140‑1 (MQ=255)
             cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           >  2:858741/1‑140 (MQ=255)
                       aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGa                                                                                                                 >  1:3099492/1‑140 (MQ=255)
                        aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  1:2356209/140‑1 (MQ=255)
                        aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  2:1688506/140‑1 (MQ=255)
                        aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACAGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTAGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  1:3704805/140‑1 (MQ=255)
                         tcTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGc                                                                                                               <  2:3736968/139‑1 (MQ=255)
                          cTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCg                                                                                                              >  2:102044/1‑140 (MQ=255)
                          cTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCg                                                                                                              >  2:1861132/1‑140 (MQ=255)
                           ttGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCgg                                                                                                             <  2:4020072/140‑1 (MQ=255)
                           gtGAGTACTTCAAAACACTTCAGGAAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATCATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCgg                                                                                                             <  2:2181502/139‑1 (MQ=255)
                            tGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGt                                                                                                            >  1:2827146/1‑140 (MQ=255)
                              aTTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  1:714277/140‑1 (MQ=255)
                                    tAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgga                                                                                                                          >  2:3961733/1‑118 (MQ=255)
                                       caccacTTGAGGTAGATGTGGTTTGGCTGACTCCGATTTAGGATGCTCCGCAGCATGATCATGGGTATGCCATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGaa                                                                                                 <  1:3802944/137‑1 (MQ=255)
                                       aaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGaa                                                                                                 <  1:858741/140‑1 (MQ=255)
                                       aaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGaa                                                                                                 <  2:3489992/140‑1 (MQ=255)
                                        aaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAg                                                                                                <  1:3729798/140‑1 (MQ=255)
                                        aaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAg                                                                                                <  2:18185/140‑1 (MQ=255)
                                        aaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAg                                                                                                <  1:3144845/140‑1 (MQ=255)
                                         acacTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGc                                                                                               <  2:1517731/140‑1 (MQ=255)
                                          cacTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCt                                                                                              <  1:2587136/140‑1 (MQ=255)
                                            cTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCa                                                                                            <  2:622100/140‑1 (MQ=255)
                                             ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                           >  1:3315688/1‑140 (MQ=255)
                                             ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                           >  1:3525144/1‑140 (MQ=255)
                                             ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCAGAAGCTCAt                                                                                           >  1:906741/1‑140 (MQ=255)
                                             ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGAGGGAGGATTTTGAGCGGTTACTGTCCGAAGCGCag                                                                                           >  1:4076000/1‑139 (MQ=255)
                                              tCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATa                                                                                          >  2:3664382/1‑140 (MQ=255)
                                               cAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATaa                                                                                         >  1:1783575/1‑140 (MQ=255)
                                                     gATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAgg                                                                                   >  1:557635/1‑140 (MQ=255)
                                                           cTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaa                                                                             >  2:123526/1‑140 (MQ=255)
                                                           cTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaa                                                                             >  1:2678823/1‑140 (MQ=255)
                                                             ttGGCTGACACCGATTTATGATTCTCCGCAGCATGATAAGGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAg                                                                           >  2:1759450/1‑140 (MQ=255)
                                                                gCTGACACCGATTTATGATTCTCCGCAGTATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgtcg                                                                        >  2:155974/1‑140 (MQ=255)
                                                                gCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          >  2:2666548/1‑106 (MQ=255)
                                                                gCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgtcg                                                                        >  2:326178/1‑140 (MQ=255)
                                                                gCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgtcg                                                                        >  1:1899545/1‑140 (MQ=255)
                                                                gCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgtcg                                                                        >  2:1909938/1‑140 (MQ=255)
                                                                    acacCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCAt                                                                    <  1:903293/140‑1 (MQ=255)
                                                                    acacCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCAt                                                                    <  2:2828336/140‑1 (MQ=255)
                                                                    acacCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCAt                                                                    <  2:2821969/140‑1 (MQ=255)
                                                                     cacCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATg                                                                   >  1:681922/1‑140 (MQ=255)
                                                                          aTTTATGATTCTCCGCTGCATGATAATGGGTATGACATTCGTGTTTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAACGTCGTCATGGATCt                                                              >  1:4170401/1‑140 (MQ=255)
                                                                          aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCt                                                              >  1:150834/1‑140 (MQ=255)
                                                                          aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCt                                                              >  2:2589563/1‑140 (MQ=255)
                                                                              ctGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACGCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAACAGGCCTGAAAGTCGTCATGGATCTTgtc                                                          <  1:2859258/139‑1 (MQ=255)
                                                                                gATTCTCCGCAGCATGATAAGGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGTTGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  2:375121/140‑1 (MQ=255)
                                                                                 cctctcCCCAGCACGATCCGGGGGATGCCCTTCGTGATTACTCCTCGATTTATCCGACAGTCGCGCCGATGGAGGCTTTTGAGCGGTTACTGGCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgtc                                                       <  2:3552733/138‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  1:1861132/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  1:1675933/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  2:4209970/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  2:224134/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  1:3013051/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCATAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  1:2949260/140‑1 (MQ=255)
                                                                                     tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATc                                                   >  1:2831306/1‑140 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  2:132240/140‑1 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  1:2412605/140‑1 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  1:1231188/140‑1 (MQ=255)
                                                                                         cagcaTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATAc                                               >  2:785362/1‑140 (MQ=255)
                                                                                           gtaTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACAt                                             <  1:155974/138‑1 (MQ=255)
                                                                                           gcaTGATAATGGGTATGACATTCTTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGTTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACAt                                             <  1:1087586/140‑1 (MQ=255)
                                                                                            caggATCATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCCTAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATc                                            >  2:275044/4‑140 (MQ=255)
                                                                                            caTGATAAGGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGTGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATc                                            >  2:2313131/1‑140 (MQ=255)
                                                                                              tGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                          <  2:2811404/140‑1 (MQ=255)
                                                                                              tGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                          <  1:2069204/140‑1 (MQ=255)
                                                                                              cagtAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                          <  1:3274029/137‑1 (MQ=255)
                                                                                                 tAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACag                                       >  2:3165464/1‑140 (MQ=255)
                                                                                                 tAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACag                                       >  2:1172837/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCTAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  2:84084/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  2:1477046/124‑1 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  >  1:1470510/1‑124 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  2:2418435/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:2086042/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:1471681/1‑140 (MQ=255)
                                                                                                        tATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                                <  1:3720691/140‑1 (MQ=255)
                                                                                                         aTGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaaa                               <  2:557635/140‑1 (MQ=255)
                                                                                                          tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGTAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGACTGAAAGTCGTCATGGATCTTGTCGTCAAGCATACATCAACAGAGCACAAAt                              >  2:1979747/1‑140 (MQ=255)
                                                                                                          tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAAt                              >  2:760199/1‑140 (MQ=255)
                                                                                                           gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                             >  1:39478/1‑140 (MQ=255)
                                                                                                           gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                             >  2:703257/1‑140 (MQ=255)
                                                                                                           gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                             >  2:1383671/1‑140 (MQ=255)
                                                                                                            aCATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCGTGAAAGTCGTCATGGATCTTGTCGTCAATCAGACATCATCAGAGctcacactg                            >  2:3274029/1‑135 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  2:3510918/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  2:2945381/1‑140 (MQ=255)
                                                                                                                  gTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  2:651070/110‑1 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  1:1979036/140‑1 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  2:867725/140‑1 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  1:3429932/140‑1 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  1:523811/140‑1 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  2:2960094/140‑1 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAGCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  2:493288/140‑1 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAAGACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  1:2173444/140‑1 (MQ=255)
                                                                                                                              cGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAATAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCg          >  2:2338677/1‑140 (MQ=255)
                                                                                                                                      ccTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGa  <  2:3430166/140‑1 (MQ=255)
                                                                                                                                      ccTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGa  <  1:631516/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GCGATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGA  >  NZ_CP034943/828650‑828923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: