Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 3,247,065 C→T intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,247,0650CT99.7% 1082.4 / NA 291intergenic (‑11/+110)bdbD/cadAdisulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (201/88);  minor base G (1/0);  total (203/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 291 total aligned reads displayed.

TCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGACG  >  NZ_CP034943/3246929‑3247199
                                                                                                                                        |                                                                                                                                      
tCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATc                                                                                                                                     >  2:1770747/1‑140 (MQ=255)
       aCCGCTTCATTGCCTTGTTCCGGTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaa                                                                                                                              >  2:732769/1‑140 (MQ=255)
                      tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               >  1:2788660/1‑140 (MQ=255)
                      tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               >  2:1621658/1‑140 (MQ=255)
                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                             >  2:3628621/1‑140 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGGCAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:472324/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:3172071/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:1235612/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:952013/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:592158/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:2685889/140‑1 (MQ=255)
                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  1:1335419/1‑140 (MQ=255)
                              tttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTACTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGc                                                                                                       <  2:2047704/140‑1 (MQ=255)
                                 gTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                    <  2:3474098/140‑1 (MQ=255)
                                  ttattaAAGATGACAATGGCTGCTACTAAGACAACAACCACAACCGTAAGCATGACCGCGAATTTCGCACAAGACTGCTGTTTCTTTTTCACTCCCACACCTTATCGATTTAAAAATAAAAAAAGAAGCGGAACGCAATa                                                                                                   >  1:3692224/1‑140 (MQ=255)
                                    attaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTGATCGTTTTAAAATTAAAAAAAGAATCGGATCGCAATAGa                                                                                                 >  2:1828609/1‑140 (MQ=255)
                                      taAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                               <  1:2497758/140‑1 (MQ=255)
                                        aaGATGACAATGGCTGCTAGTCAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGATTTAAAATTAAAAAAAGAAGCGGATCGCAAGAGACCCa                                                                                             <  2:3201325/140‑1 (MQ=255)
                                        aaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCa                                                                                             <  2:1251801/140‑1 (MQ=255)
                                             gACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtct                                                                                        >  2:4166189/1‑140 (MQ=255)
                                             gACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtct                                                                                        >  2:2647694/1‑140 (MQ=255)
                                                 ctGGCTGCTACTAAGACAACAACCACAACCGTAAGGATGACCGCGAATGTCGCAGAAGCCTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                    <  2:3267398/139‑1 (MQ=255)
                                                 aTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                    <  1:1181207/140‑1 (MQ=255)
                                                     ctgctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGGTTTAAAATTAAAAAAAGAAGAGGATCGCAATAGAACCACTTCTTTATTTTa                                                                                >  2:3520432/1‑140 (MQ=255)
                                                      tgctAGTAAGACAACAACCACAACCGTAAGGATGACCGCTAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTaa                                                                               <  1:3431308/140‑1 (MQ=255)
                                                      tgctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTaa                                                                               <  2:3396273/140‑1 (MQ=255)
                                                       gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCACTAGACCCACTTCTTTATTTTAac                                                                              >  1:1457648/1‑140 (MQ=255)
                                                        ctAGTAAGACAACAACCACAAGCGTAAGGATGACCGCGAATTTCGCAGTAGACTGCTGTTTGTTTTTCACTCCGACACGTTATCGTTTTCAAATTTAAATAAGAAGCGGATGGCAAGAGACGCTCGTGTTTAtttttagt                                                                             >  2:2508173/1‑136 (MQ=255)
                                                        ctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATATTAaca                                                                             >  2:3978819/1‑140 (MQ=255)
                                                          aggaaGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTGAACATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaag                                                                           >  1:80125/4‑140 (MQ=255)
                                                          aGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaag                                                                           >  2:2839114/1‑140 (MQ=255)
                                                            tAAGACAACCACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagac                                                                         <  1:3093085/140‑1 (MQ=255)
                                                            tAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagac                                                                         <  2:2219346/140‑1 (MQ=255)
                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                       <  2:1138421/140‑1 (MQ=255)
                                                                   acaaccacacccGGAAGGATGACCGCGAAGTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                                  <  2:787036/140‑1 (MQ=255)
                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                    >  2:52568/1‑105 (MQ=255)
                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCAATTCTTTATTTTAACAAGACAAGTGACt                                                                 >  2:3464659/1‑140 (MQ=255)
                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCATTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCTCAATAGACACACTTCTTTATTTTAACAAGACAAGTGCCt                                                                 >  1:272592/1‑140 (MQ=255)
                                                                       ccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCtata                                                              >  1:268168/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                           >  2:1693897/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                           >  1:1113220/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                           >  2:711522/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                           >  1:632773/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                           >  1:3251619/1‑140 (MQ=255)
                                                                            accGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaaa                                                         >  1:2882916/1‑140 (MQ=255)
                                                                            accGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaaa                                                         >  1:3482616/1‑140 (MQ=255)
                                                                               gTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAcc                                                      >  2:3690352/1‑140 (MQ=255)
                                                                               gTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAcc                                                      >  2:1368863/1‑140 (MQ=255)
                                                                                tAAGGATGTCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAccc                                                     >  2:3170473/1‑140 (MQ=255)
                                                                                tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCa                                                                                                      <  1:2172997/91‑1 (MQ=255)
                                                                                tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAccc                                                     >  1:245625/1‑140 (MQ=255)
                                                                                tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAccc                                                     >  2:4086440/1‑140 (MQ=255)
                                                                                tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAccc                                                     >  1:76198/1‑140 (MQ=255)
                                                                                tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAAAAAAccc                                                     >  1:3791670/1‑140 (MQ=255)
                                                                                     aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                               <  1:1939944/93‑1 (MQ=255)
                                                                                     aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                               >  2:1939944/1‑93 (MQ=255)
                                                                                     aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaa                                                >  1:3970471/1‑140 (MQ=255)
                                                                                     aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaa                                                >  2:2865366/1‑140 (MQ=255)
                                                                                       gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTCTCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCGACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                              >  2:1419499/1‑140 (MQ=255)
                                                                                       gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                              >  2:4262661/1‑140 (MQ=255)
                                                                                       gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                              >  1:868797/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGTAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1395/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAATATAAGCGGATCGCAATAGACCAACTTCTTTATTTTAAAAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:2972004/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:271915/79‑1 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:1022460/79‑1 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          >  2:1022460/1‑79 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                    >  2:2096053/1‑85 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                    <  1:2096053/85‑1 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaacaaa                                             >  2:2899556/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:703123/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:3565614/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1727916/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1614583/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:1541902/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:4209180/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:1534069/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:3088944/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:983132/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:2407691/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGGTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:3037409/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTCCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAACCGGATCGCAATAGACCCACTTCATTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:2334669/1‑140 (MQ=255)
                                                                                            cgAATTTCGCAGCAGACGGCTGTTTCTTTGTCACTCCGACACCTTATCGATTTAAAATTAAAAAAAGAAGCGGAGCGCAATAGACCCACTTCTTTATTTTAACCCGACACGTGCCTATCAGACAACCCCGAAAAAATGaa                                         <  1:1997244/140‑1 (MQ=255)
                                                                                            cgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGaa                                         <  1:1539197/140‑1 (MQ=255)
                                                                                                      agaagaCTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGa                               <  1:2839114/140‑1 (MQ=255)
                                                                                                             tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                        >  1:125810/1‑140 (MQ=255)
                                                                                                             tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                        >  2:919005/1‑140 (MQ=255)
                                                                                                             tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTATCTCCGACAATTTa                        >  1:1770316/1‑140 (MQ=255)
                                                                                                             tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCACGAAAAAATGAATTCTCTCCGCCAATTTa                        >  1:3306915/1‑140 (MQ=255)
                                                                                                               ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATc                      <  2:2997569/140‑1 (MQ=255)
                                                                                                               ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATc                      <  2:100030/140‑1 (MQ=255)
                                                                                                                    tCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAATAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACACCGAAAAAATGAATTCTCTCCGACAATTTATcctgtt                 >  1:3554020/1‑136 (MQ=255)
                                                                                                                       ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATtct                                     <  2:2159985/117‑1 (MQ=255)
                                                                                                                       ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg              >  2:4055257/1‑140 (MQ=255)
                                                                                                                        tttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga             >  1:3887071/1‑140 (MQ=255)
                                                                                                                         ttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTgag            <  2:1482247/140‑1 (MQ=255)
                                                                                                                               ccGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCAt      <  2:371020/140‑1 (MQ=255)
                                                                                                                                cGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTATTTATGTTAACAAGACAAGTGCCTATAAGAAAACCACGAAAAAATTAATTCTCTCCTACAATTTATCCGTGTTTGAGACGCATg     >  1:1506149/1‑140 (MQ=255)
                                                                                                                                 gACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGa    <  2:899766/140‑1 (MQ=255)
                                                                                                                                 gACACCTTAGCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAAAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACACTTTATCCGTTTTTGAGACGCATGa    <  2:2282616/140‑1 (MQ=255)
                                                                                                                                  acacCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGAc   >  2:467858/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGACG  >  NZ_CP034943/3246929‑3247199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: