Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 704,350 Δ1 bp intergenic (+172/‑12) EO946_RS03650 → / → EO946_RS03655 CamS family sex pheromone protein/phosphotransferase enzyme family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943704,3480A.99.3% 732.1 / 0.3 150intergenic (+170/‑14)EO946_RS03650/EO946_RS03655CamS family sex pheromone protein/phosphotransferase enzyme family protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (61/87);  total (63/87)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 174 total aligned reads displayed.

GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGA  >  NZ_CP034943/704209‑704483
                                                                                                                                           |                                                                                                                                       
gCACAAGGGTTTTTTTATGTTACAATTAGCAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         <  2:1333902/140‑1 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         >  1:2840191/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         >  2:1524958/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         >  1:1541022/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         >  2:1716043/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         >  2:1124928/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         >  2:105392/1‑140 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                        <  2:2305551/140‑1 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                        <  2:50490/140‑1 (MQ=255)
 aacaATGGTTTTTTTATGTTACAATTAACAGTAATAGGAAACAGATTTATTTTTGATTCTGCCGAGACACAAATCATGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                        <  2:1080369/139‑1 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:2249916/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:790403/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:1461256/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:809494/140‑2 (MQ=255)
  acaAGGGATTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:3029045/140‑2 (MQ=255)
    aaGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAggg                                                                                                                                     >  2:459675/1‑140 (MQ=255)
      gggTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAg                                                                                                                                  >  1:2697588/1‑140 (MQ=255)
       ggTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGt                                                                                                                                 <  1:204311/140‑1 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                             <  2:736209/140‑1 (MQ=255)
                 tGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCa                                                                                                                       >  1:1178621/1‑140 (MQ=255)
                      cAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAg                                                                                                                  <  1:2904751/140‑1 (MQ=255)
                           aaCAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAACTCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATc                                                                                                             <  1:1384311/140‑1 (MQ=255)
                              aGGAATAGGAAAAATATTTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCaaa                                                                                                          <  1:176695/140‑1 (MQ=255)
                              aGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGAc                                                                                                                            <  1:1160294/122‑1 (MQ=255)
                              aGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCaaa                                                                                                          <  1:2510964/140‑1 (MQ=255)
                                gAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCaaaaa                                                                                                        >  2:962145/1‑140 (MQ=255)
                                gAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGCAGTTAGACGTCCATAAAGAAATCaaaaa                                                                                                        >  2:138319/1‑140 (MQ=255)
                                   tAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACat                                                                                                     >  1:1514110/1‑140 (MQ=255)
                                        aaaaGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTc                                                                                                >  2:1231969/1‑140 (MQ=255)
                                          aaGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCAt                                                                                              >  2:2133413/1‑140 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             >  2:1746580/1‑140 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        >  1:300332/1‑140 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        >  1:903935/1‑140 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        <  1:1054508/140‑1 (MQ=255)
                                                     ttGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGt                                                                                   >  2:2138222/1‑140 (MQ=255)
                                                       gCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGAAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGttt                                                                                 >  1:2141511/1‑140 (MQ=255)
                                                          tCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGAc                                                                                                                            <  2:2174147/94‑1 (MQ=255)
                                                            tGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGaa                                                                                                                >  2:1475876/1‑104 (MQ=255)
                                                            tGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCa                                                                            <  2:1834983/140‑1 (MQ=255)
                                                            tGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCa                                                                            >  2:2217410/1‑140 (MQ=255)
                                                            tGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCa                                                                            <  1:1746580/140‑1 (MQ=255)
                                                             gCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCag                                                                           <  2:2537018/140‑1 (MQ=255)
                                                                 agaCACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGc                                                                       <  2:1918219/140‑1 (MQ=255)
                                                                    cacaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGc                                                                    <  1:679900/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                   <  1:2733599/140‑1 (MQ=255)
                                                                              ggggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATAc                                                          >  2:1114987/1‑140 (MQ=255)
                                                                               gggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACg                                                         <  1:1296327/140‑1 (MQ=255)
                                                                                 gAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGt                                                       >  2:888853/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                     >  1:1002117/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                     >  1:1004880/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                     >  2:653078/1‑140 (MQ=255)
                                                                                    gTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTtc                                                    >  1:16837/1‑140 (MQ=255)
                                                                                        gTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        >  2:2491292/1‑100 (MQ=255)
                                                                                        gTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        <  1:2491292/100‑1 (MQ=255)
                                                                                        gTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaa                                                <  2:1541022/140‑1 (MQ=255)
                                                                                        gTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaa                                                >  2:1184251/1‑140 (MQ=255)
                                                                                         ttGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaaa                                               >  2:389318/1‑140 (MQ=255)
                                                                                           gATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCACAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACa                                             >  1:2571858/1‑140 (MQ=255)
                                                                                            aTGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAg                                            >  1:1511077/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGa                                                                  <  2:369097/116‑1 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGa                                                                  >  1:369097/1‑116 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  1:2391133/1‑140 (MQ=255)
                                                                                               cTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        <  1:520500/93‑1 (MQ=255)
                                                                                               cTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCa                                         <  2:1002117/140‑1 (MQ=255)
                                                                                                   aaCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTg                                     <  2:300332/140‑1 (MQ=255)
                                                                                                   aaCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTg                                     <  2:2739846/140‑1 (MQ=255)
                                                                                                    aCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGc                                    <  1:642785/140‑1 (MQ=255)
                                                                                                       cGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGtt                                 >  2:2606917/1‑140 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACGGCCAGGTGCGtttt                               >  1:2010068/1‑140 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGtttt                               <  2:607146/140‑1 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGtttt                               <  2:2840191/140‑1 (MQ=255)
                                                                                                                aCAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                        <  1:1723994/140‑1 (MQ=255)
                                                                                                                aCAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                        <  1:2274531/140‑1 (MQ=255)
                                                                                                                aCAATCAATATCAAATCAAGAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                        <  2:2936995/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGACGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                <  2:2805720/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                <  1:1124928/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:848340/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:2194815/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:1980213/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:962145/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  2:441758/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:2301581/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:1419859/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:2964104/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  1:1095084/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa              <  2:1787377/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGACGGGAGTTAGACGTGCATAAAGAAATCAAAAACATAGTTCATGAAGATCAGGTATTGGCAGAGGCTGCGATGAAATACGGTTTCTCAACCAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1455292/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:2606917/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1370862/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1623159/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATATCATGAAGATCAGGTTTTGGCAGAGGCTGCGATTAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  2:723846/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:3076973/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:718064/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:1435494/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          <  2:1584917/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAACAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGCCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:2452975/1‑140 (MQ=255)
                                                                                                                                  aTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGACGATCAGGTTTTGGCAGAGTCTGCGATGAAATACGGTTTATCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGtat      >  2:631119/1‑140 (MQ=255)
                                                                                                                                    aGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTatat    <  1:434937/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACGGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa  <  2:2010068/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGACTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAAAACGGTTTCTCAAACAGCCAGGTGCGTTTTATGGCTGATGCGGAAAACTATGTATATGa  >  2:1596038/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGA  >  NZ_CP034943/704209‑704483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: