Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,270,0520GC26.3% 232.7 / 23.3 136L43V (CTT→GTT) prsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base G (55/43);  new base C (0/35);  total (55/81)
Fisher's exact test for biased strand distribution p-value = 1.43e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 136 total aligned reads displayed.

CACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAAC  >  NZ_CP034943/2269920‑2270186
                                                                                                                                      |                                                                                                                                      
cacTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAAcc                                                                                                                                 <  2:3017410/140‑1 (MQ=255)
   tGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACCACCCCt                                                                                                                              >  2:544401/1‑140 (MQ=255)
   tGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCt                                                                                                                              >  2:988113/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                                                                                                     >  1:661067/1‑131 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  2:2234848/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  1:2168438/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  1:2125253/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  2:2351974/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  1:1625583/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  1:2540155/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  1:1261241/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                            >  2:1409072/1‑140 (MQ=255)
     aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAACCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAAAAACCCCTAg                                                                                                                            >  2:2186280/1‑140 (MQ=255)
     aCCATCAGAATAAAACATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCAAGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCAAg                                                                                                                            >  1:818288/1‑140 (MQ=255)
          cAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                                                                                                     >  2:1111295/1‑126 (MQ=255)
                  aCCATTTTAATGATTCCTCCCAACACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGa                                                                                                               >  1:828108/1‑140 (MQ=255)
                   catttttAATGATCCCACCAAAAACCC‑TCAACATAAAAAAAAAACCAAAACGCTTCCGCCTCCCACATT‑CTCTTTTACAAGCACTTAGTGGAAAAACTTTGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGaa                                                                                                              <  1:1347041/137‑1 (MQ=255)
                    ctttttAATTATTCCTCCAAAAACCC‑TTACCATAAAAAATAAACCAAAACGCTTCCCCCTCCCAATTT‑CTATTTTTCAAGCACGTACTGGATAAACTTTGTGGTAAATACAAGAACAACCCCTATAAAAAACGACCGAAg                                                                                                             <  1:407186/138‑1 (MQ=255)
                    cATTTTAATGATCCCTCAAAAAACCG‑TCAATATAAAAAACAAACTAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTAAAGCACGTACTGGATAAACTTTGTGGTAAATACAATAACACCCCCTAGAAAAAACGACCGAAg                                                                                                             <  1:123264/140‑1 (MQ=255)
                     tttttAATTTTTCCTCAAAACCCCC‑TCAATAAAAAAAATAAACTAAAACGCTTCCGCCGCCCAATTG‑CTCTTTTTCAAGCACGTACTGTAAAAACTGTGGGGTAAAAACAACAACAACCCCTACAAAAAACGACCGAAGc                                                                                                            <  1:1409072/139‑1 (MQ=255)
                     tttttAATGTTTCCTCCAAAAACCC‑TGAATATAAAAAATAAACGAAAAAGCTTCCGCCACCCACATT‑CTCTTTTTCAAGCCCGTACTGCAAAAACTTTGTGGGAAATAAAACAACACCCCCTACAAAAAACGACCGAAGc                                                                                                            <  1:2792316/139‑1 (MQ=255)
                     tttttAATCATTCCCCAAAAACCCC‑TGACTATAAAAAAAAAACCAAAAAGCTTCCGCCTCCAACTTT‑CTCTTTTTCAAGCACGTCGTGTATAACCATTGTGGGAAATACAACAACAACCCCTACAAAAAACGACCGAAGc                                                                                                            <  1:2322805/139‑1 (MQ=255)
                     aTTTTATTGATTCCTCCAAAAACCC‑TGAAAATAAAAAAGAAACCAAAAAGATTCCGCCACCCACATT‑CTCTTTTTCAAGCACTTACTGTATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                            <  2:1289873/140‑1 (MQ=255)
                     aTTTTAATGATTCCACCAAAACCCC‑TCAATATAAAAAACAAACCCAAAAGCTGCCGCCTCCCAATTT‑CTCTTTTAAAAGCACTTACTGGTTAAACATTGTGGGAAATACAACAACAACCCCTCCAAAAAACGACCGAAGc                                                                                                            <  2:828108/140‑1 (MQ=255)
                     aTTTTAATGATTCATCCAAAAACCC‑TGAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCAAATT‑ATCTTTTTCACGCACTTACTGGATAAAAATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                            <  2:2353379/140‑1 (MQ=255)
                     aTTATAATGATTCATCCAAAAACCC‑TGAATATAAAAAACAAACGAAACAGCTTCCGCCTCCCAAATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                            <  1:2224810/140‑1 (MQ=255)
                       tttATTGATTCCTCAAAAAACCC‑TTAATATAAAAAAGAAACTAAAAAGCTTCCGCCTCCCACTTT‑CTATTTTTCAAGCACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                          <  2:3029412/140‑1 (MQ=255)
                       tttAATTATTCCTCCAAAAACCA‑TGAAGATAAAAAAGAAACCAAAACGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTTTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                          <  2:1907861/140‑1 (MQ=255)
                        ttATTGATACCACAAAAAACCC‑TCAATATAAAAAATAAACCAAAACGCGGCCGCCTCCCAAATT‑ATCTTTTTAAAGCACGTACTGGATAAACTTTGTGGTAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCAcc                                                                                                         <  1:274463/140‑1 (MQ=255)
                        ttAATTTTTCCTCAAAAAACCA‑TCAATATAAAAAATAAACGAAAAAGCTTCCGCCTCCCATATT‑CTCTTTTTAAAGCACTTACTGTAAAAACATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCAcc                                                                                                         <  1:2076351/140‑1 (MQ=255)
                        ttAATGATTCATCAAAAAACCC‑TGAATATAAAAAATAAACCAAAAAGCTTCCGCCTCCCACATT‑CTATTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                         <  2:2830326/140‑1 (MQ=255)
                        ttAATCATTCCACAAAAAACCA‑TAAATAAAAAAAAAAAACCAAAAAGCTTCCGCCTCCCACATT‑ATATTTTTAAAGCACGTACTGTATAAAATTTGTGGTAAATACAACAACAACCCCTACAAAACACGACCGAAGCAcc                                                                                                         <  1:2746874/140‑1 (MQ=255)
                         tAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGTAAATACAAGAACAACCCCTAGAAAACACGACCGAAGCACCa                                                                                                        <  1:3074825/140‑1 (MQ=255)
                          aatttttCCTCCAAAAACCC‑TTAAGATAAAAAAGAAACGAAAACGCTGCCGCCTCCCAAATT‑CTATTTTTCAAGCCCGTACTTGATAAACTTTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                       <  2:2374745/135‑1 (MQ=255)
                          aaTTATTCCTCCAAAAACCC‑TGAATATAAAAAATAAACCAAAAAGGTTCCGCCTCCCACATT‑ATCTTTTTAAAGCACGTACTGTATAAAATTTGGGGTAAATACAATAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                       <  2:786921/140‑1 (MQ=255)
                          aaTGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTATTTTTCAAGCACGTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                       <  2:1863189/140‑1 (MQ=255)
                          aaTGATTCCCCCAAAAACCC‑TTAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATA‑CTCTTTTTCACGCACGTACTGGATAAACATTGTGGCAAATACACGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                       <  2:1504526/140‑1 (MQ=255)
                           aTGATTCCTCCAAAAACCC‑TTAACATAAAAAAAAAACGAAAACTCTTCCGCATCCAAAATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGTTAAATACAACACCAACCCCTAGAAAAAACGACCGAAGCACCata                                                                                                      <  2:898816/140‑1 (MQ=255)
                              aTTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGt                                                                                                   >  2:2897602/1‑140 (MQ=255)
                               ttCCTCCAAAAACCC‑TGAATATAAAAAAGAAACGAAAAAGCTACCGCCACCAACATA‑CTCTTTTTAAAGCACGTACTGGATAAAAATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTa                                                                                                  <  2:320835/140‑1 (MQ=255)
                               ttCCTCCAAAAACCC‑GTAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTa                                                                                                  <  2:3034287/140‑1 (MQ=255)
                               ttCCTCAAAACACCC‑TGAAGATAAAAAATAAACCACAACGCGCCCGCCTCCCACATT‑CTGTTTTTCAAGCACGTACTGGATAAAAATTGTGGTAAAAAAAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTa                                                                                                  <  1:1621842/140‑1 (MQ=255)
                                tcctccAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                 <  2:2125253/140‑1 (MQ=255)
                                ccacaaaaaaaCCC‑TCAACAAAAAAAACAAACCAACAAGCTGCCCCCTCCCACATT‑CTATTTTACACGCACGTACTGTATAAACATTGGGGTAAAAACAACAACAACCCCTCCAAAAAACGACCGAAGCACCATATGTAc                                                                                                 <  1:118767/134‑1 (MQ=255)
                                    ccAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGt                                                                                             >  2:960829/1‑140 (MQ=255)
                                     caaaccccc‑TGAACATAAAAAACAAACCAAAAAGCTTCCGCCTCCCAAATT‑CTCTTTTTCAAGCACTTACTGCATAAACATTGTGGGAAATACAAGAACAACCCCTCCAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                            <  2:2910172/134‑1 (MQ=255)
                                     cAAAAACCC‑TTAATATAAAAAACAAACCAAAAAGCTTCCGCATCCCACATT‑CTATTTTTAAAGCACGTACTGTATAAACTTTGTGTGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                            <  2:1936049/140‑1 (MQ=255)
                                     cAAAAACCC‑TGAACATAAAAAAAAAACCAAAAAGCTACCGCCTCCCACATT‑CTATTTTTCAAGAACGTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                            <  2:1049696/140‑1 (MQ=255)
                                     cAAAAACCC‑TCACTATAAAAAATAAACCAACCTGGTCCCGCCTCCCACATC‑TTATTTTTCAAGCACGTACTGGATAACGTTGGTGGAAAATACACTAACAACCCCTACAAAAAACGACCGAAGCACCATATGGACAGGtt                                                                                            <  1:2321140/140‑1 (MQ=255)
                                     aaaaacCCC‑TCAAGATAAAAAATAAACCAAAACGCGTCCGCCTCCCACATT‑CTCTTTTTAAAGCCCGTACTGTATAAAATTTGTGGGAAATACAACAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                            <  1:787419/139‑1 (MQ=255)
                                     aaaaaaCCC‑TGAATAAAAAAAATAAACCAAAAAGCTTCCGCCTCCCAATTT‑CTATTTTTAAAGCACGTACTGTATAAAAATTGTGGTAAATAAAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                            <  1:2133102/139‑1 (MQ=255)
                                     aaaaaaCCC‑TGAAGATAAAAAAGAAACTAAAAAGCTTCCGCCTCACACATT‑CTCTTTTTCAAGCACTTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                            <  2:2540155/139‑1 (MQ=255)
                                     aaaaaaCCA‑TTAATAAAAAAAACAAACCAAAAAGCTTCCGCCCCCCACTTT‑ATATTTTTAAAGCCCGTACTGGATAAACTTTGTGGTAAATAAAACAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                                                                                            <  1:988113/139‑1 (MQ=255)
                                      aaaaaCCCATCAAGAAAAAAAAAAAACCAAAAAGCTACCCCCACCC‑CATTGATCTTTTTCAAGCCCGTATTGGATAAAATTTGTGGCAATTCCAACAACACCCCCACCAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                            <  1:57981/140‑1 (MQ=255)
                                        acaCCC‑TTAATATAAAAAATAACCCAAAAAGCGCCCGCCCCCCACATT‑CTCTTTTTCAAGCCCGTAGTGTATAACCTTGGTGGCAAATAAAACAACAACCCCTACAAACCACGACCGAAGCACCATATGTACAGGTTCAt                                                                                         <  1:544401/138‑1 (MQ=255)
                                         aaCCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCAtt                                                                                        <  2:337238/140‑1 (MQ=255)
                                            cc‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTctgtctc                                                                                     >  2:561035/1‑134 (MQ=255)
                                             c‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATct                                                                                    >  2:1488958/1‑139 (MQ=255)
                                                      aaaaaaGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATc                                                                            >  2:712124/1‑140 (MQ=255)
                                                       aaaaaGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCATGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTGCATTATCCTACTGATCt                                                                           >  2:2507500/1‑140 (MQ=255)
                                                       aaaaaGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCt                                                                           >  1:429992/1‑140 (MQ=255)
                                                       aaaaaGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCt                                                                           >  1:1022354/1‑140 (MQ=255)
                                                       aaaaaGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATAGGTACAGGTTCATTATCATACTGATCt                                                                           >  1:228816/1‑140 (MQ=255)
                                                                 gAAAACTCTTCCGCCACCTACATT‑CTCTTTTAAAAGCACGTAATGCATAAACATTGTGTTAAATACAACAACAACCCATAGAAAAAACGACCGAATCACCATATGTACAGGTTCATTATCATACCGATCTTTtaaataaa                                                                 <  2:1448843/140‑1 (MQ=255)
                                                                 gAAAACTCTACCGCCTCCCACATT‑CTCTTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCAATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                 <  2:70180/140‑1 (MQ=255)
                                                                 gAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                 <  2:2635565/140‑1 (MQ=255)
                                                                 cacaaaGCTTCCCCCTCCCACATT‑CTCTTTTTAAAGCACGTACTGCATAAACCTTGTGGGAAATACACCAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                 <  1:2496383/137‑1 (MQ=255)
                                                                   aaacGCTACCGCCACCCACTTT‑CTATTTTTAAAGCACTTACTGTATAAACATTGTGGGAAATACAACAACAACCCCAAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTaaataaaaa                                                               <  1:3062996/140‑1 (MQ=255)
                                                                   aaaaGCTTCCGCCTCCCACTTT‑CTCTTTTTCAAGCACGTACTGGATAAACATTTTGGTAAATAAAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTaaataaaaa                                                               <  2:1413936/140‑1 (MQ=255)
                                                                           ccGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTa                                                                                                  <  2:1681350/97‑1 (MQ=255)
                                                                                 cccaattt‑CTCTTTTTCAAGCACGTCCTGCATAAACTTTGTGGCAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAgcgc                                                 <  1:1101282/134‑1 (MQ=255)
                                                                                 cccACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                  >  1:675427/1‑75 (MQ=255)
                                                                                 cccACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                  <  2:675427/75‑1 (MQ=255)
                                                                                 cccACATT‑CTATTTTTCAAGAACGTACTGTATAAACATTGTGTGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAgcgc                                                 <  1:1097197/140‑1 (MQ=255)
                                                                                  ccACATT‑CTCTTTTTCAAGCACGTACTGGATAAAAATTGTGGTAAATACAACAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGcgcg                                                <  1:2517794/140‑1 (MQ=255)
                                                                                             tttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAg                                      >  2:2731408/1‑140 (MQ=255)
                                                                                             tttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAg                                      >  2:1227300/1‑140 (MQ=255)
                                                                                              ttttCAAGAACTTACTGTATAAACATTGTGGGAAAAACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                     <  1:2602850/140‑1 (MQ=255)
                                                                                              tttaCAAGAACGTACTGTATAAACATTGTGGGAAATACCACACCACCCCATAGAAAAAACGACCGAAGCACCATCTGTACAGGTTCAGTATCATACTGATCTTTTAAATAAAAATAACTGAACAGCGCGATGCCGGGAGc                                     <  1:2597739/140‑1 (MQ=255)
                                                                                                 tCAAGAACGTCCTGGATAAACATGGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                  <  2:1261241/140‑1 (MQ=255)
                                                                                                          tACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCaga                         <  1:2959101/140‑1 (MQ=255)
                                                                                                            cTGTATAAAAATTGTGGCAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAgaga                       <  1:960829/140‑1 (MQ=255)
                                                                                                               gATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgatga                    >  2:1721468/1‑140 (MQ=255)
                                                                                                                 tAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAtt                  >  1:653797/1‑140 (MQ=255)
                                                                                                                 tAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAtt                  >  1:1644820/1‑140 (MQ=255)
                                                                                                                   aaaaTTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGc                <  2:332830/137‑1 (MQ=255)
                                                                                                                    ccATGGTGGAATAAACAACACCACCCCCTCTAAAACACGACCGAAGCACCTTCGGTCCAGCGTCATTATCAGACTGATCTTTTAAATAAAAATAACTTACCAGCGCGATGACGGGAGCAATTCCTGCAGAGATGATTGCa               <  1:1455656/139‑1 (MQ=255)
                                                                                                                    actttGTGCGAAATACAACAACCACCCCTAGAAAACACGACCGAAGCACCATCTGTACAGGTTCAGTATCCTACTGATCTTTTAAATAAAAATAACTTACCAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa               <  1:2193473/137‑1 (MQ=255)
                                                                                                                    aaaTTTTGGTAAATACACGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa               <  1:206458/138‑1 (MQ=255)
                                                                                                                    aaaTTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa               <  1:501711/138‑1 (MQ=255)
                                                                                                                    aCATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGTTTCATTATCATTCTGATCTTTTATATAAAAATAACTTAAGAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa               >  2:2042543/1‑140 (MQ=255)
                                                                                                                    aCATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATCAAAATAACTTAACAGCGCGAAGCCGGGAGCACTTCCTGCCGAGATGTGTGCa               >  2:1993431/1‑140 (MQ=255)
                                                                                                                       ttGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAAc            <  1:996020/140‑1 (MQ=255)
                                                                                                                         gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt          >  2:590815/1‑140 (MQ=255)
                                                                                                                         gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt          >  2:506175/1‑140 (MQ=255)
                                                                                                                         gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt          >  2:501249/1‑140 (MQ=255)
                                                                                                                         gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt          >  1:2230769/1‑140 (MQ=255)
                                                                                                                         gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt          >  2:1584293/1‑140 (MQ=255)
                                                                                                                         gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt          >  2:2519955/1‑140 (MQ=255)
                                                                                                                              aaaTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGc     <  2:1649358/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCC‑TGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATT‑CTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAAC  >  NZ_CP034943/2269920‑2270186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑