Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 132,186 A→G D560G (GAC→GGC)  fusA → elongation factor G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943132,1860AG99.6% 957.6 / NA 271D560G (GAC→GGC) fusAelongation factor G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (147/123);  minor base T (0/1);  total (147/124)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322
                                                                                                                                        |                                                                                                                                        
caCGAAGTAGGCGCGTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCAt                                                                                                                                       <  2:2364992/139‑1 (MQ=255)
aaCGAAGATGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCAt                                                                                                                                       <  2:1592580/140‑1 (MQ=255)
aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCAt                                                                                                                                       >  1:2036122/1‑140 (MQ=255)
aaCGAAGAAGGCGCTTGCTGTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCAt                                                                                                                                       >  2:856332/1‑140 (MQ=255)
       aagGCGCTTGCTTTTAATTTGAAAATGCGATTGTCGGTTGGGTAGTTCCACGTTAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACGGGCATGCTTCCCGCTTATCGGCATTAAAGCa                                                                                                                                <  2:2294063/140‑1 (MQ=255)
        agGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCaa                                                                                                                               <  1:1917151/140‑1 (MQ=255)
                 cttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCg                                                                                                                      <  1:986924/140‑1 (MQ=255)
                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  1:3078947/140‑1 (MQ=255)
                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:544975/140‑1 (MQ=255)
                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:63542/140‑1 (MQ=255)
                         ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              <  1:1392000/140‑1 (MQ=255)
                         ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              <  1:2230160/140‑1 (MQ=255)
                         ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAAGACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              <  2:2621685/140‑1 (MQ=255)
                           gaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAATATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATAGGCATTAAAGCAAAACTTTTCGATGGTTCTTa                                                                                                            <  1:1349146/140‑1 (MQ=255)
                              aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  2:431043/140‑1 (MQ=255)
                              aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  1:385996/140‑1 (MQ=255)
                                               ggTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACg                                                                                        >  2:2851118/1‑140 (MQ=255)
                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGa                                                                                                >  2:1617937/1‑127 (MQ=255)
                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   <  1:712680/140‑1 (MQ=255)
                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   <  1:1337886/140‑1 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              <  2:1145374/108‑1 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              >  1:1145374/1‑108 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:586495/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:300418/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  2:31002/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:758850/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  2:2933796/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1755471/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:2647053/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1692798/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:188574/1‑140 (MQ=255)
                                                              aTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTATATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTaaat                                                                         >  1:2518143/1‑139 (MQ=255)
                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     <  1:1359849/140‑1 (MQ=255)
                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     <  1:2092601/140‑1 (MQ=255)
                                                                   tCCGAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  1:2159358/140‑1 (MQ=255)
                                                                   tCCCAGCTGTTCTAGGAGGTCTTGAAGATGATCATGTAAAGGGTTTACTGGCAGTCTTCAGGCTGTTCGGCATTAAATCACAACTTTTAGAGGGTTGTTACCATGATGTTGACGCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:2032361/140‑1 (MQ=255)
                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:2802992/140‑1 (MQ=255)
                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:2261002/140‑1 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  2:1491858/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  2:2726049/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  1:2931264/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  2:276743/1‑140 (MQ=255)
                                                                     ccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctgc                                                                  <  1:2787385/140‑1 (MQ=255)
                                                                     ccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctgc                                                                  <  1:2263117/140‑1 (MQ=255)
                                                                       aGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCatgatg                                                                                                    >  2:2697141/1‑104 (MQ=255)
                                                                       aGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCtt                                                                >  1:2765410/1‑140 (MQ=255)
                                                                       aGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCtt                                                                >  2:1780425/1‑140 (MQ=255)
                                                                          tGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTa                                                             <  1:1780425/140‑1 (MQ=255)
                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:2309807/1‑140 (MQ=255)
                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGAGGTTGACACAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:1852261/1‑140 (MQ=255)
                                                                              cAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGCTGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGc                                                         >  2:1282983/1‑140 (MQ=255)
                                                                                   aGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGa                                                    >  2:1553994/1‑140 (MQ=255)
                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:2885397/1‑140 (MQ=255)
                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:487723/1‑140 (MQ=255)
                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATGTAAAGTTGCTGCTGATATGGCAGTGaaaa                                                 >  1:198049/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     >  1:54959/1‑63 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGt                                                                                                   <  2:2426478/81‑1 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:815388/112‑1 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                                                      <  2:1852487/126‑1 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCTGtga                                        >  2:1654017/1‑138 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:988673/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2544191/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2644624/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:4205/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:3012087/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1327103/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:271558/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:300145/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1222041/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:786168/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:898205/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:2735189/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATCATGTTGACTCAAACGAAATGGCATTTAAACTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2470833/1‑140 (MQ=255)
                                                                                                gCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg                                       >  2:66018/1‑140 (MQ=255)
                                                                                                 cACTTGAAAATGGTTTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  1:1070146/140‑1 (MQ=255)
                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  1:2901776/1‑111 (MQ=255)
                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  2:333333/140‑1 (MQ=255)
                                                                                                     tGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAAt                                  >  2:1189280/1‑140 (MQ=255)
                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:2226276/140‑1 (MQ=255)
                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  1:2429223/140‑1 (MQ=255)
                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:2612891/140‑1 (MQ=255)
                                                                                                            ggTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAAccc                           <  1:2851118/140‑1 (MQ=255)
                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                         <  2:500457/60‑1 (MQ=255)
                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg                                       >  1:2580131/1‑126 (MQ=255)
                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                         >  1:560730/1‑140 (MQ=255)
                                                                                                               gtACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  2:562465/140‑1 (MQ=255)
                                                                                                                tACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 >  2:1942905/1‑130 (MQ=255)
                                                                                                                tACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       <  2:898003/140‑1 (MQ=255)
                                                                                                                     gCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCCGTCAGCAAATGTACCCCAGTtcttct                  >  2:1923898/1‑140 (MQ=255)
                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  2:2951908/1‑140 (MQ=255)
                                                                                                                          cTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc             <  2:763040/140‑1 (MQ=255)
                                                                                                                                 cTTATCGGCATTAAAGCACAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  1:1082592/140‑1 (MQ=255)
                                                                                                                                 cTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  1:876945/140‑1 (MQ=255)
                                                                                                                                 cTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  2:1692798/140‑1 (MQ=255)
                                                                                                                                 cTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  1:1576549/140‑1 (MQ=255)
                                                                                                                                 attATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  2:1985047/139‑1 (MQ=255)
                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGACATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   >  2:1445933/1‑140 (MQ=255)
                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  2:2390439/119‑1 (MQ=255)
                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        >  1:2390439/1‑119 (MQ=255)
                                                                                                                                     tCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaaa  >  2:1262742/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: