Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 828,787 G→A E77K (GAA→AAA)  treC → alpha,alpha‑phosphotrehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943828,7870GA98.0% 573.4 / 0.1 156E77K (GAA→AAA) treCalpha,alpha‑phosphotrehalase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (74/77);  total (77/79)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 156 total aligned reads displayed.

GATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGA  >  NZ_CP034943/828652‑828923
                                                                                                                                       |                                                                                                                                        
 aTCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATAc                                                                                                                                     >  2:50433/1‑140 (MQ=255)
 aTCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATAc                                                                                                                                     >  2:1430859/1‑140 (MQ=255)
 aTCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGAGTATTACTCGATTTATCCTAAATAc                                                                                                                                     >  2:50426/1‑140 (MQ=255)
           cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCTTGATTATTACTCGATTTATCCTAAATACTGGACGATgg                                                                                                                           >  2:473590/1‑140 (MQ=255)
           cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGAggg                                                                                                                           >  2:2029851/1‑140 (MQ=255)
           cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           >  2:1479131/1‑140 (MQ=255)
           cATTATTGAAAAGCTGGATTACTTAAAAACGCTACAGGTGGATGTGCTTTGGCTGACGCCGATTTATGATTCTCCCCAGCATGATAATGGGTACGATATTCGTGATTATTACTCGATTTATCCTGAATACGGAACGATgg                                                                                                                           >  1:3049158/1‑140 (MQ=255)
                    aaaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTg                                                                                                                  <  2:2426104/140‑1 (MQ=255)
                     aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACTGGACGATGGAGGATTTTGa                                                                                                                 >  1:1893621/1‑140 (MQ=255)
                     aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGa                                                                                                                 >  2:135713/1‑140 (MQ=255)
                     aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGa                                                                                                                 >  1:2911072/1‑140 (MQ=255)
                      aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGCCACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  2:2524075/140‑1 (MQ=255)
                      aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  2:1758770/140‑1 (MQ=255)
                      aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  2:1679976/140‑1 (MQ=255)
                      aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  1:1944386/140‑1 (MQ=255)
                      aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  1:910307/140‑1 (MQ=255)
                      aGCTTGATGACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  2:2700558/140‑1 (MQ=255)
                            aTTACTTACAAACACTTCAGGTAGAAGTGCTTGGGCTGCCACCGATTTATGAGTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  1:1579745/140‑1 (MQ=255)
                            aTTACTTAAAACCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGCTTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  1:2623016/140‑1 (MQ=255)
                            aTTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  1:1082152/140‑1 (MQ=255)
                                  tAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCATAAAAACTGGACGATGTAGGATTTTGAGCGGTTACTGTcc                                                                                                    >  2:1614144/1‑140 (MQ=255)
                                   aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                   <  1:11900/140‑1 (MQ=255)
                                   aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                   <  1:2002381/140‑1 (MQ=255)
                                   aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                   <  1:1024697/140‑1 (MQ=255)
                                     acacacTTCCGGTAGACGTGCTTTGGCTGACACCGATTGAAGAGTCTCCGCAGCATGATCATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGaa                                                                                                 <  1:984917/138‑1 (MQ=255)
                                     aaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGaa                                                                                                 <  1:2097591/140‑1 (MQ=255)
                                     aaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGaa                                                                                                 <  1:1373085/140‑1 (MQ=255)
                                           ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                           >  1:857544/1‑140 (MQ=255)
                                           ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                           >  1:2044233/1‑140 (MQ=255)
                                           ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGAATATTACTCGATTTATCCTAAATACGGGACGAGGGAGGATTTTGAGCGGTTACTGTCCGCAGCTCAt                                                                                           >  1:1745056/1‑140 (MQ=255)
                                                     tgtgCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGAGTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGcc                                                                                 >  2:1945287/1‑140 (MQ=255)
                                                       tgCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                   <  1:1656756/120‑1 (MQ=255)
                                                       tgCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                   >  2:1656756/1‑120 (MQ=255)
                                                         cTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaa                                                                             >  2:2881806/1‑140 (MQ=255)
                                                         cTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaa                                                                             >  1:2038157/1‑140 (MQ=255)
                                                         cTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaa                                                                             >  1:1252456/1‑140 (MQ=255)
                                                         cTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaa                                                                             >  1:1937210/1‑140 (MQ=255)
                                                          tttGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaaa                                                                            >  1:2543673/1‑140 (MQ=255)
                                                          tttGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaaa                                                                            >  1:2491462/1‑140 (MQ=255)
                                                          tttGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaaa                                                                            >  2:281973/1‑140 (MQ=255)
                                                           ttGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAg                                                                           <  2:2911072/140‑1 (MQ=255)
                                                            tGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCTATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgt                                                                          <  1:1605314/140‑1 (MQ=255)
                                                            tGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgt                                                                          <  1:480932/140‑1 (MQ=255)
                                                                tGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgtcgtc                                                                      <  2:2433708/140‑1 (MQ=255)
                                                                tGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGAGTATTACTCGATTTATCCTCAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgtcgtc                                                                      <  2:2960315/140‑1 (MQ=255)
                                                                 gACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATCAAAGAGGCCTGAAAGTCGTCa                                                                     >  1:2047852/1‑140 (MQ=255)
                                                                       gATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATc                                                               <  2:2756247/140‑1 (MQ=255)
                                                                       gATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATc                                                               <  2:2510939/140‑1 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACgg                                                                                                                                   <  2:2165805/71‑1 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACgg                                                                                                                                   >  1:2165805/1‑71 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgt                                                                          >  2:2862413/1‑128 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCCTGGATCt                                                              >  1:828994/1‑140 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCt                                                              >  1:2301266/1‑140 (MQ=255)
                                                                            aTGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtc                                                          <  1:651116/140‑1 (MQ=255)
                                                                              gagtctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACGGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:2751327/137‑1 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        >  1:3047942/1‑140 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:2623248/140‑1 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:512451/140‑1 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:512294/140‑1 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:1297490/140‑1 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCTATTTATCCTAACTACGGGACGATGGAGGATTTTGTGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATTGATCTTGTCGTCAAt                                                    <  1:1713292/140‑1 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    >  1:1025016/1‑140 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    >  2:1065344/1‑140 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  1:616408/140‑1 (MQ=255)
                                                                                   tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCGTAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:1778060/135‑1 (MQ=255)
                                                                                   tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCGTAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        >  2:1778060/1‑135 (MQ=255)
                                                                                   tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATc                                                   >  2:371505/1‑140 (MQ=255)
                                                                                   tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATc                                                   >  1:1082213/1‑140 (MQ=255)
                                                                                    ccGCAGCATGATACTGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  1:2220312/140‑1 (MQ=255)
                                                                                    ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  2:2732481/140‑1 (MQ=255)
                                                                                         gcaTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACAt                                             <  1:1430859/140‑1 (MQ=255)
                                                                                          caTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATc                                            >  1:2628632/1‑140 (MQ=255)
                                                                                          caTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATc                                            >  2:2246300/1‑140 (MQ=255)
                                                                                            tGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                          >  2:2248154/1‑140 (MQ=255)
                                                                                            tGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                          <  1:23922/140‑1 (MQ=255)
                                                                                             gATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAag                                                                                      >  2:116860/1‑95 (MQ=255)
                                                                                              aTAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACa                                        <  1:2508652/140‑1 (MQ=255)
                                                                                               tAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACag                                       >  1:1986567/1‑140 (MQ=255)
                                                                                                  tGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGc                                    >  2:2783415/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  2:2135830/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:207192/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:1328944/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  2:1163748/1‑140 (MQ=255)
                                                                                                      tctGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAGCATACATCAACAGAGCACaa                                >  1:536162/3‑140 (MQ=255)
                                                                                                      tATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                                <  1:257878/140‑1 (MQ=255)
                                                                                                      tATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                                <  1:1308900/140‑1 (MQ=255)
                                                                                                        tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAg                                                                                                >  2:47423/1‑74 (MQ=255)
                                                                                                        tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAAt                              >  1:1344404/1‑140 (MQ=255)
                                                                                                         gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        >  1:2757092/1‑113 (MQ=255)
                                                                                                         gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                             >  2:434044/1‑140 (MQ=255)
                                                                                                              tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  2:753160/108‑1 (MQ=255)
                                                                                                               cGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCa                       >  1:61143/1‑140 (MQ=255)
                                                                                                                    ttattaCTCGAGTTATCCTAACTACGGGACGTTGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATATTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAgg                  <  1:50426/140‑1 (MQ=255)
                                                                                                                      attaCTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCg                >  1:675505/1‑140 (MQ=255)
                                                                                                                          ctctATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  2:2124413/140‑1 (MQ=255)
                                                                                                                          cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  2:1354913/140‑1 (MQ=255)
                                                                                                                          cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCAGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  2:668162/140‑1 (MQ=255)
                                                                                                                               tttATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCt       <  2:970833/140‑1 (MQ=255)
                                                                                                                                   tCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAg   <  2:3049894/140‑1 (MQ=255)
                                                                                                                                    ccTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTAGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGa  <  1:1479131/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGA  >  NZ_CP034943/828652‑828923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: