Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 135,162 C→A 6.7% intergenic (+209/‑29) EO946_RS00715 → / → rpsJ alpha/beta hydrolase/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943135,1620CA 6.7% 1056.8 / 18.4 426intergenic (+209/‑29)EO946_RS00715/rpsJalpha/beta hydrolase/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base C (185/195);  new base A (42/2);  total (227/199)
Fisher's exact test for biased strand distribution p-value = 2.31e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 442 total aligned reads displayed.

AAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATCT  >  NZ_CP034943/135023‑135301
                                                                                                                                           |                                                                                                                                           
   tCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCAGGGCAAATGTGTTGAGGTTTTTATCACGGGGTACTGTGTAAAttt                                                                                                                                          >  1:1311912/1‑139 (MQ=255)
   tCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTg                                                                                                                                          >  2:4052748/1‑140 (MQ=255)
       gcagcaTACGTCTATTTTCTGTATAATGGTGTATGTTGGGCTTTTCCTTCGATGAAGTGAGAGGTTGCTGACACTCCCGGCCGCTTTGCCTTGGCAAACGTGGTCATGTATTTCACACTCAGAAATGTCTAACATgctct                                                                                                                                      >  1:4056592/1‑136 (MQ=255)
       gCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCATGGCAAAGGTGTTCAGGTTTTTGTGACGGAGAACTGTCTAACTtgttgt                                                                                                                                      >  1:4527244/1‑140 (MQ=255)
       gCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGAGGt                                                                                                                                      >  2:234226/1‑140 (MQ=255)
            aTACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTAAGGTTTTTGTGACGGAGAACTGTGTAACTTGATGTATGCg                                                                                                                                 >  1:4872122/1‑140 (MQ=255)
             tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCACAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGa                                                                                                                                >  2:2943984/1‑140 (MQ=255)
               cGTGTATTTTCTGTATAATGGTGTATGTTGGTCTTGGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGaaa                                                                                                                              <  2:849407/140‑1 (MQ=255)
                          tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCCAAGGTGTTGAGGTTTTTATAACGGAGAACTGTCTAACTTTATGTAGGGGAAAAGGAGGGaaaa                                                                                                                   >  1:3368497/1‑140 (MQ=255)
                          tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATTGCAAACGTGTTAAGTTTTTTATAAAGGAGAAAAGTATAAATTGATGTAGGGGAAAAGGAGGGaaaa                                                                                                                   >  1:1932006/1‑140 (MQ=255)
                          tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTCCCTGGCCAACGGGTTAATGTTTTTATAAAGGAGAACAGAGTAACTTGTTTTATGCGAACAGGAGGGaaaa                                                                                                                   >  1:2040954/1‑140 (MQ=255)
                           gTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGTTTTTTGCGCAAAGGTGGGTAaat                                                                                                                  >  2:386720/1‑140 (MQ=255)
                            tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCAAGGCACAGGTGTTAAGGTTTTTATCACGGAGAACTGTCTAAATTGTTGTAGGCGAAAAGGAGGGGAaata                                                                                                                 >  1:1413363/1‑140 (MQ=255)
                            tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCCTGGGAAAGGTTTTCAGGTTTTTATCACGGAGAACTGTCTAACTTGATGTGTGCGAAAAGGAGGGAAaata                                                                                                                 >  1:2869269/1‑140 (MQ=255)
                            tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAAGGCAAAAGTGTTCACGTTTTTATCAAGGAGAAAAGAGTAACTTTATTTAAGCGAAAAAGAGGGaaaaaa                                                                                                                 >  1:2339140/1‑138 (MQ=255)
                              taATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACAGTCTAACATTATGTAGGCGAAAAGGAGGGAaaaaaaa                                                                                                               >  1:1491265/1‑139 (MQ=255)
                                 tGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGc                                                                                                            <  1:220124/140‑1 (MQ=255)
                                    tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAAATTGATTTAGGCGAAAAGGAGGGAAAATAATGGcaaa                                                                                                         >  2:5130956/1‑140 (MQ=255)
                                    tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaa                                                                                                         >  2:167500/1‑140 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATTGCAAAGGTGTTCAGGTTTTTATAACGGAGAACTGTCTAACTTGATTTAGTCGAAAAGGAGGGAAAATAAATGcaaaac                                                                                                       >  1:2647031/1‑140 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGGACAGGGGGTCAGGTTTTTCTCACGGAGAACTGTGGACCTTGATGTTGGCGAAAAGGAGGGAAAATAATGGcaaaac                                                                                                       >  1:2765452/1‑140 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAAGGCAAAGGTGTTCAGGTTTTTCTAACGGAGAACTGTGGAACTTGATTTAGGCGAAAACGAGGGAAAATAAAGGcaaaaa                                                                                                       >  1:3773755/1‑139 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGCCAGGGCACAGGTGTTGAGGTTTTTGTCACGGAGAACTGTATAACATGATGTTGGGGCAAAGGACGGGAAAAAATGGcaaaac                                                                                                       >  1:1613507/1‑140 (MQ=255)
                                         gTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGTTGTTCAGGTTTTTCTCACGGAGAACTGTCTAAATTGATGTAGGCGAAAAGGAGGGAAAAAAATGGcaaaacaaa                                                                                                    >  2:191922/1‑140 (MQ=255)
                                           tGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGt                                                                                                                                      >  2:2147362/1‑104 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATTGCAAAGGTGTTAAGGGTTTTATCACGGAGAACTGTCTAACATTATTTAGGCGGAAAGGATGGAAAATAAAGGCAAAACaaaaaaa                                                                                                >  1:3456172/1‑139 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATTGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAat                                                                                                >  2:1065820/1‑140 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCAAGGCAAAGGTGTTCAGGTTTTTTTAAAGGAAAAAAGTGGAAAATTATGTAGGGGAAAATGAGGGAAAATAAATGCAAAAAAAAAAat                                                                                                >  1:3990814/1‑140 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAat                                                                                                >  2:4774019/1‑140 (MQ=255)
                                             gTCTTTGACTGCGATGAAGGGAGAGGTTGCTGCCACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCCGGTTTTTCTCACGGAGCACGGGCGAACATGATGTAGGCGCACAGGAGGGAAAATCATGGCAACCCaaaaaag                                                                                                >  2:1377857/1‑139 (MQ=255)
                                              tCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAatt                                                                                               <  1:2819171/140‑1 (MQ=255)
                                                tttGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTTATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattag                                                                                             >  2:924543/1‑138 (MQ=255)
                                                  tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACCGAGAACTGTCTAACATGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAAAAAAAAattcgaa                                                                                           >  2:3218470/1‑138 (MQ=255)
                                                         gATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCCGGTTTTTCTCACGGAGAACTTTATAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGttt                                                                                    >  2:448547/1‑140 (MQ=255)
                                                           tGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGa                                                                                  <  2:2875814/140‑1 (MQ=255)
                                                                tGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCAAGGTGTTCAGGTTTTTGTAAAGGAGAACAGTCGAAATTGATGTAGGCGAAAAGGAGGGAAAATAAATGCAAAACAAAAAAATAGTATTAGTTTTAAAGCa                                                                             >  1:3713526/1‑140 (MQ=255)
                                                                  agagGTTGCTGACACACCCTGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                                                                           <  2:2184506/140‑1 (MQ=255)
                                                                  agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                                                                           <  2:175141/140‑1 (MQ=255)
                                                                  agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                                                                           <  2:1834034/140‑1 (MQ=255)
                                                                  agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGATTGAAAGCata                                                                           <  2:4149251/140‑1 (MQ=255)
                                                                   gcggTTGCTGACACCCCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTATCTCACTGAGAACTGTCAAACTTTATGTAGGCGAAAATGACTGCAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCatat                                                                          <  1:2153190/138‑1 (MQ=255)
                                                                   gagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCatat                                                                          <  2:2040954/140‑1 (MQ=255)
                                                                   gagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCatat                                                                          <  2:275557/140‑1 (MQ=255)
                                                                   cagcgtgctgcCACACCCGCCCGCTTTGCCAGGGCAAAGGTGTTCACGGTTTTCTCACGGCGAACTGTCTAACTGGAGGTAGGCGACAAGGCGGGAAACTAATGCCAAAACAAAACATTCGGATTCGTTTGAAAGCatat                                                                          <  1:4449125/135‑1 (MQ=255)
                                                                    agGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTAAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATg                                                                         >  2:664213/1‑140 (MQ=255)
                                                                       ttGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATc                                                                      >  2:246254/1‑140 (MQ=255)
                                                                          cTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCCGGTTTTTCTCACGGAGAACTGTCTAAGTTGATGTAGGCGAAAAGGAGGTAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATa                                                                   <  2:2244872/140‑1 (MQ=255)
                                                                          cTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATa                                                                   <  1:5151041/140‑1 (MQ=255)
                                                                           tGACACACCCGGCCGCTTTGCTATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                                                                  <  2:4938556/140‑1 (MQ=255)
                                                                           tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                                                                  <  1:1031118/140‑1 (MQ=255)
                                                                           tGACACACCCGGCCGCTGTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                                                                  <  1:1533653/140‑1 (MQ=255)
                                                                                      gCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATc                                                       <  2:605549/140‑1 (MQ=255)
                                                                                              gCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCa                                               <  1:2518289/140‑1 (MQ=255)
                                                                                                 aTGGCAAAGGTGTTCAGGATTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCagag                                            <  1:3575879/140‑1 (MQ=255)
                                                                                                         ggTGTTAAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGAttgt                                    >  2:42853/1‑140 (MQ=255)
                                                                                                             ttCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGTGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGACGATTGTTGaa                                >  1:3059332/1‑140 (MQ=255)
                                                                                                             ttCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGaa                                >  2:1133826/1‑140 (MQ=255)
                                                                                                             ttCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGaa                                >  1:660097/1‑140 (MQ=255)
                                                                                                             ttCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGGAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATATGAAGAGAATATTGTTGaa                                >  1:461292/1‑140 (MQ=255)
                                                                                                             ttCAGGTTTATCTCACGGAGAACTGTCTAACTTGATGTAGGCGAACAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGaa                                >  1:2382841/1‑140 (MQ=255)
                                                                                                              tCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGaaa                               >  1:933303/1‑140 (MQ=255)
                                                                                                               cAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAAc                              >  1:2725081/1‑140 (MQ=255)
                                                                                                                     tttCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGTAACGGCaaa                        >  2:4603172/1‑140 (MQ=255)
                                                                                                                     tttCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCaaa                        <  2:3456172/140‑1 (MQ=255)
                                                                                                                           actgagaACTGTATAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATGAGTAGTCGTTTGAAAGCATATGATCATAGAATCCTTGATCTATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:3503257/137‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  2:2786929/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  2:338237/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  2:3713526/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:1085327/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  2:1458642/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  2:1194634/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:3230409/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:1761061/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:4121454/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:4774019/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTAGATGTAGGCGAAAAGGATGGAACAAAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:1725966/140‑1 (MQ=255)
                                                                                                                            cGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCt                 >  1:120662/1‑140 (MQ=255)
                                                                                                                                gaACTTTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  2:767864/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  2:3927682/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  2:5027500/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  1:4634238/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  1:5002767/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  1:709193/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGCCAAAACTAAAAATTCGTATTCGATTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  2:3607279/140‑1 (MQ=255)
                                                                                                                                 aaCTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGc            <  1:4910059/140‑1 (MQ=255)
                                                                                                                                 aaCTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGc            >  1:4312957/1‑140 (MQ=255)
                                                                                                                                    tGTCTAACTTGATGTAGGCGAACAGGAGGGAAAATAATGGCAAAACAAAAATTTCTTCTTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAg         <  2:3773755/140‑1 (MQ=255)
                                                                                                                                    tGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAg         <  2:3980902/140‑1 (MQ=255)
                                                                                                                                    tGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAg         <  1:388314/140‑1 (MQ=255)
                                                                                                                                    tGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAg         <  1:837712/140‑1 (MQ=255)
                                                                                                                                    tGTCTAACTGGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAg         <  2:3010693/140‑1 (MQ=255)
                                                                                                                                    ggTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAACACTTCTTATGCGTTTGAAAGCATATGATCATAGCATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGGGCTAg         <  1:3009653/139‑1 (MQ=255)
                                                                                                                                      tCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAgtg       >  2:3321006/1‑140 (MQ=255)
                                                                                                                                          aCTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATc   <  2:1545008/140‑1 (MQ=255)
                                                                                                                                          aCTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATc   <  2:725539/140‑1 (MQ=255)
                                                                                                                                          aCTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATc   <  2:2647031/140‑1 (MQ=255)
                                                                                                                                          aCTTGATGTAGGAGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATc   <  2:4030967/140‑1 (MQ=255)
                                                                                                                                           cTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  1:3622638/129‑1 (MQ=255)
                                                                                                                                           cTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTATTGTATcg  >  1:4541931/1‑139 (MQ=255)
                                                                                                                                           cTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATCt  >  1:4252340/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATCT  >  NZ_CP034943/135023‑135301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: