Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,359 A→C 8.9% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,3590AC 8.9% 601.2 / 13.3 284T68P (ACC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (106/138);  new base C (4/30);  total (112/172)
Fisher's exact test for biased strand distribution p-value = 2.80e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498
                                                                                                                                          |                                                                                                                                           
    cGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCACCCGGAGCGACGGGAGCGACGGGAGCTACCGGCGAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGa                                                                                                                                        <  2:1642111/140‑1 (MQ=17)
     gggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAg                                                                                                                                       <  1:2495436/140‑1 (MQ=255)
          cTACTGGGTAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:2848416/140‑1 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:2096541/140‑1 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCggagcgacgggagcgacg                                                                                                                                  >  2:2503168/1‑140 (MQ=255)
            aCTGGGTAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  1:4508803/140‑1 (MQ=255)
            aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACGGGAGCGACggg                                                                                                                                <  1:3565209/140‑1 (MQ=255)
               ggggAAGCTGGACCAGCCGGACCAGCCGGAGCGCCGGGAGCCACCGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGCGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGGCGCAACCGGCGCGCCGGgcgc                                                                                                                             >  1:495627/1‑140 (MQ=17)
                gcgAAGCTGGACCAGCCGGACCACCGGGAGCGACGGGAGCCACTGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGcc                                                                                                                            <  1:19172/138‑1 (MQ=255)
                 ggAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCa                                                                                                                           <  1:3903416/140‑1 (MQ=255)
                  gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  2:41903/140‑1 (MQ=255)
                  gAAGCTGGACCAGACGGACCAGCCGGAGCGACGGGACCCACGGGAGCTACCGGTGAAGCTGGACCAGCCGGAGCCACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  2:805348/140‑1 (MQ=21)
                    aGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacgggagcgacgg                                                                                                                        >  2:2793622/1‑140 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCCACCGGCGCGACCGGGGCGAgggg                                                                                                                       >  2:707918/1‑140 (MQ=14)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  1:2572439/1‑140 (MQ=255)
                       tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGCAGCCACGGGAg                                                                                                                     >  1:2617229/1‑140 (MQ=255)
                       tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAg                                                                                                                     >  2:1856490/1‑140 (MQ=255)
                        ggaccagccggaccagccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGc                                                                                                                    <  1:337018/140‑1 (MQ=255)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  2:2205163/140‑1 (MQ=255)
                              gccggtcccgccggcGCGACGGCGCGCCGGGAGCTACCGGTGAAGCGGGACCAGCCGGAGCGTCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGTCGGGAGCCACGGGAGCTACCggg                                                                                                             <  2:5019624/140‑1 (MQ=17)
                               ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  2:2567993/140‑1 (MQ=255)
                               ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  1:3066540/140‑1 (MQ=255)
                                   accagccggaGCGCGCGGAGCCACCGGACGTACCGGGGCAGCCGGACCAGCCGGACCGCCGGGAGCCACAGGACCTCCCGGGGAACCCGGACCACCCGGACCCACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  2:4704786/140‑1 (MQ=14)
                                   accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACGGGAGCCACGGGGGCGACGGGCGCAGCCggacaag                                                                                                         >  1:2067195/1‑135 (MQ=11)
                                       gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCCGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGACCTACCGCGGaccccgc                                                                                                     >  1:2932496/1‑134 (MQ=18)
                                       gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCACCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACAGGAGCGACCGGAGCGACGGCAGCTACCGGGGAagccgg                                                                                                     >  1:4437983/1‑140 (MQ=18)
                                        ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGTACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                                                                                                    <  1:3655337/140‑1 (MQ=255)
                                            aGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  2:938505/140‑1 (MQ=255)
                                            aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  1:1090940/140‑1 (MQ=255)
                                            aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                >  2:1724527/1‑140 (MQ=255)
                                            aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  1:1423978/140‑1 (MQ=255)
                                            aGCGACGGGAGCCACCGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  2:4998967/140‑1 (MQ=255)
                                             gCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAagccggaccagc                                                                                               >  1:632242/1‑140 (MQ=255)
                                             gCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagc                                                                                               >  1:166355/1‑140 (MQ=255)
                                              cGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagcc                                                                                              <  1:1654126/140‑1 (MQ=255)
                                                aCGGGAGCCACGGGAGCTCCCGGTGCAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg                                                                                            <  1:4301141/140‑1 (MQ=255)
                                                aCGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg                                                                                            <  1:1724527/140‑1 (MQ=255)
                                                aCGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg                                                                                            <  1:3919937/140‑1 (MQ=255)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAATCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  1:4939901/140‑1 (MQ=255)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  1:673927/140‑1 (MQ=255)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACCGGGGAACCCGGAGCAGCCGGACCACCCGGAGCGACCGGAGCGACGGGAGCGACCGGGGCagccgggcctggcgga                                                                                           >  2:1479461/1‑140 (MQ=17)
                                                    gAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAgccggaccagccggacca                                                                                        <  1:4909458/140‑1 (MQ=255)
                                                      gCCACGGGAGCTACCGGCGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  2:366903/140‑1 (MQ=255)
                                                       ccACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGACCCACCGGAGCGACGGGCGCGACGGGTGCGAGCGGGGCCgccggaccagccggaccaccg                                                                                     >  2:2233139/1‑137 (MQ=18)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  1:1504757/140‑1 (MQ=255)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagagaca                                                                            >  2:1231221/1‑140 (MQ=255)
                                                                  aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:1166923/140‑1 (MQ=255)
                                                                  aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:4845488/140‑1 (MQ=255)
                                                                    cGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                        <  2:4418558/140‑1 (MQ=255)
                                                                       ggAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                     <  1:2161576/140‑1 (MQ=255)
                                                                       ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                     <  1:5104560/140‑1 (MQ=255)
                                                                       ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                     <  1:4159383/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:3804590/140‑1 (MQ=255)
                                                                          aGCTGGACCAGCCGGAGCGACGGGAGGGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAACAACCGgagcgacaggagcgacag                                                                  <  1:4660911/140‑1 (MQ=255)
                                                                          aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCggagcgacaggagcgaccg                                                                  >  2:4513767/1‑138 (MQ=255)
                                                                          aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                  <  1:4789687/140‑1 (MQ=255)
                                                                          aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGGCCACCCGgagcgacgggcgcgacag                                                                  >  2:3175305/1‑140 (MQ=17)
                                                                           gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                 <  1:1750186/140‑1 (MQ=255)
                                                                             tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:1080140/140‑1 (MQ=255)
                                                                             tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCCACCGGAGCGACCGGCGCAACCGGCCACGCCGGCACAGCCCGACCAACCGgaccgagaggggcgagagcgc                                                               >  2:3999540/1‑137 (MQ=14)
                                                                              ggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCgacaggagcgacaggagc                                                              <  1:4216657/140‑1 (MQ=255)
                                                                              ggACCAGCCGGACCGCCGGGAGCGCCGGGAGAGACCGGCGCACCCGGAGCACCAGGACCACCCGGAGCCACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCgacaggagcgacaggagc                                                              <  1:197097/140‑1 (MQ=14)
                                                                               gACCAGCCGGAGCTACGGGAGCCACCGGAGCTACCGGGGAAGCTGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCt                                                             <  2:873377/140‑1 (MQ=255)
                                                                               gACCAGCCGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCt                                                             <  2:2668791/140‑1 (MQ=255)
                                                                                tccAGCCGGAGCCCCGGGAGCGACAGTATCTACGGGGTCCGCCGGCCCAGCGGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  2:635878/139‑1 (MQ=17)
                                                                                aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCCACGGGAGCTACCGGGGACGCCGGAGCCACCGGACCAACCGGAGCgaccggcgcgacaggagcga                                                            >  2:3429716/1‑138 (MQ=17)
                                                                                aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCAc                                                                                                                 >  2:3558267/1‑87 (MQ=255)
                                                                                    gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        <  2:3283573/140‑1 (MQ=255)
                                                                                    gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGCAGCTCCCGGCGCAGCCGGACCAGCGCGAGCACGCGGTGCGACAGGAGGGACAGGAGCGGCCgg                                                        >  1:4563665/1‑140 (MQ=14)
                                                                                     ccGGAGCGCCGGGCGCGACCGGAGCCACCGGGGCCGCCGGACCAGCCGGCCCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:4039851/140‑1 (MQ=14)
                                                                                         cgCGACGGGAGCGACTGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  2:3023504/139‑1 (MQ=255)
                                                                                         aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  2:192647/140‑1 (MQ=255)
                                                                                         aGCGACGGGAGCGACAGGAGCTACCGGGGAACCCGGCCCAGCAGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  2:4321977/140‑1 (MQ=255)
                                                                                              cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCCGGCCCAGCCGGACCAACCGGAGCGACCGGAGCGACCGGCGCTCCCGGGGAAGCCGGa                                              >  1:3220840/1‑140 (MQ=17)
                                                                                              cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                              >  1:2379277/1‑140 (MQ=255)
                                                                                               gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             >  2:4576026/1‑140 (MQ=255)
                                                                                               gggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  2:1727239/140‑1 (MQ=255)
                                                                                                    ccacgggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGACCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        <  1:2727883/135‑1 (MQ=14)
                                                                                                    cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACTGGAGCTACCGTGGACGCCTGACCAGCCCGACCAACCTCAGAGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        >  1:4388650/1‑140 (MQ=17)
                                                                                                    cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        <  2:3923335/140‑1 (MQ=255)
                                                                                                            gCTACCGGTGAAGCCGGACCAGCCGGACCAACGGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgac                                <  2:30940/140‑1 (MQ=255)
                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               >  2:137176/1‑108 (MQ=255)
                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  1:137176/108‑1 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGGGCGACAGGAGCGCCCGGGGAAGCCGGACCAGCCggagcgaccgg                             >  2:3408282/1‑140 (MQ=17)
                                                                                                                     gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAATCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCACCCGGAGCAGCCGGAGCAGCCggagcgacaggagcgac                       >  1:2279277/1‑140 (MQ=17)
                                                                                                                     gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                       <  1:281978/140‑1 (MQ=255)
                                                                                                                     gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGCCggagcgacaggagcgac                       >  1:1988917/1‑140 (MQ=255)
                                                                                                                        gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        >  1:1241587/1‑120 (MQ=255)
                                                                                                                        gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        <  2:1241587/120‑1 (MQ=255)
                                                                                                                             accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               >  1:3428424/1‑140 (MQ=255)
                                                                                                                             accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               >  1:3885678/1‑140 (MQ=255)
                                                                                                                              gcagccggcccaCCCGGAGGGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  2:3225883/139‑1 (MQ=17)
                                                                                                                                  ccggaccaGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga          >  2:2707092/1‑140 (MQ=255)
                                                                                                                                  ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgaccggagcggcggga          >  1:1588602/1‑140 (MQ=255)
                                                                                                                                    gcaccaACCGCAGCGACGGCAGCCACGGGCGCGACCGCGGAAGCCGGACCAGCCGGACCAACCGGAGCGCCAGGAGCGACACGAGCTACCGGGGAAGCCGGACCAGCGGGAGCgacaggagcgacaggagcgacaggagc        <  1:908387/138‑1 (MQ=17)
                                                                                                                                     gaccaCCCGGAGCGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt       <  1:3864892/140‑1 (MQ=255)
                                                                                                                                     gaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt       <  1:558602/140‑1 (MQ=255)
                                                                                                                                          aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGTCCGGAGCTACAGGGGTCGCCGGACCCGCCGGTGCGACAGGTGCGCCAGGCGCGCGAGGTGCTACCgg  >  1:5166578/1‑140 (MQ=17)
                                                                                                                                          aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg  >  1:2635490/1‑140 (MQ=255)
                                                                                                                                          aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgc  >  1:730427/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: