Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,379 G→C 5.7% T74T (ACG→ACC EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,3790GC 5.7% 698.6 / 3.2 265T74T (ACG→ACCEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (97/144);  new base C (11/7);  total (110/155)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

AGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGG  >  NZ_CP034943/607241‑607516
                                                                                                                                          |                                                                                                                                         
aGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacgggagcgacgg                                                                                                                                          >  2:2793622/1‑140 (MQ=255)
 gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCCACCGGCGCGACCGGGGCGAgggg                                                                                                                                         >  2:707918/1‑140 (MQ=14)
 gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacgggagcgacggg                                                                                                                                         >  2:3661952/1‑140 (MQ=255)
 gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                                         >  1:2572439/1‑140 (MQ=255)
 gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACAAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACAGGAGCGACGGGAGCCACggg                                                                                                                                         >  2:2858165/1‑140 (MQ=255)
   tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGCAGCCACGGGAg                                                                                                                                       >  1:2617229/1‑140 (MQ=255)
   tGGACCAGCCGGACCAGCCGGAGCGACCGGAGCCACGGGAGCCACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACagggg                                                                                                                                       <  2:1547316/140‑3 (MQ=21)
      accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                                    <  2:2205163/140‑1 (MQ=255)
      accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCAACCGGAGCTACGGGAGCCACCGGAGcga                                                                                                                                    >  1:5069218/1‑138 (MQ=18)
           ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                                               <  1:1384738/140‑1 (MQ=255)
           ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                                               >  2:2577229/1‑140 (MQ=255)
               accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACGGGAGCCACGGGGGCGACGGGCGCAGCCggacaag                                                                                                                           >  1:2067195/1‑135 (MQ=11)
                   cccggaGCGACGGGAGCCACGGGAGCTCCCGGTGAAGCTCGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgg                                                                                                                       <  2:1837252/139‑1 (MQ=255)
                        accGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                                  <  2:4999243/138‑1 (MQ=255)
                        aGCGACGGGCGCCACGGGAGCCACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                                  <  2:459498/140‑1 (MQ=255)
                        aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                                  <  1:1090940/140‑1 (MQ=255)
                        aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                                  >  2:1724527/1‑140 (MQ=255)
                         gCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAagccggaccagc                                                                                                                 >  1:632242/1‑140 (MQ=255)
                         gCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagc                                                                                                                 >  1:4940360/1‑140 (MQ=255)
                          cGACGGGAGCCACGGGAGCTACCGGCGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagcc                                                                                                                <  1:4859688/140‑1 (MQ=255)
                            aCGGGAGCCACGGGAGCTCCCGGTGCAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg                                                                                                              <  1:4301141/140‑1 (MQ=255)
                             ggggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                                             <  1:52293/139‑1 (MQ=255)
                             cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                                             <  1:673927/140‑1 (MQ=255)
                             cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                                             >  1:1694474/1‑140 (MQ=255)
                                   ccACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGACCCACCGGAGCGACGGGCGCGACGGGTGCGAGCGGGGCCgccggaccagccggaccaccg                                                                                                       >  2:2233139/1‑137 (MQ=18)
                                     aggggAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGTAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                                     <  1:3061215/138‑1 (MQ=255)
                                            cTACCGGGGAAGCTGGACCAGCCGGAGCGAGGGGAGCGAAAGGAGCTACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                                              <  1:3258472/140‑1 (MQ=255)
                                            cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                                              <  1:1956978/140‑1 (MQ=255)
                                            cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagagaca                                                                                              >  2:1231221/1‑140 (MQ=255)
                                              aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                                            <  1:1166923/140‑1 (MQ=255)
                                              aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACGCGGACCAACCTGACCGACCCGAGCAACGGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCggagcgacagg                                                                                            >  2:3192898/1‑140 (MQ=18)
                                                   ggAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                                       <  1:2161576/140‑1 (MQ=255)
                                                   ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGCACCACCCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACCggagcgacaggagcga                                                                                       >  1:2446485/1‑140 (MQ=14)
                                                   ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                                       <  1:4159383/140‑1 (MQ=255)
                                                   ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCAGGACCAACCggagcgacaggagcga                                                                                       <  1:3151550/140‑1 (MQ=255)
                                                   ggAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                                       <  1:2371040/140‑1 (MQ=11)
                                                    gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                      <  1:3804298/140‑1 (MQ=255)
                                                    gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                      <  1:1980934/140‑1 (MQ=255)
                                                      aGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                                    <  1:5163138/140‑1 (MQ=255)
                                                      aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCggagcgacaggagcgaccg                                                                                    >  2:4513767/1‑138 (MQ=255)
                                                       gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGCCCAACCGGagcgacaggagcgacagg                                                                                   >  2:3048214/1‑140 (MQ=255)
                                                       gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                   <  1:1750186/140‑1 (MQ=255)
                                                         tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                 <  2:1080140/140‑1 (MQ=255)
                                                         tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCCACCGGAGCGACCGGCGCAACCGGCCACGCCGGCACAGCCCGACCAACCGgaccgagaggggcgagagcgc                                                                                 >  2:3999540/1‑137 (MQ=14)
                                                          ggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCgacaggagcgacaggagc                                                                                <  1:4216657/140‑1 (MQ=255)
                                                           gACCAGCCGGAGCTACGGGAGCCACCGGAGCTACCGGGGAAGCTGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCt                                                                               <  2:873377/140‑1 (MQ=255)
                                                            tccAGCCGGAGCCCCGGGAGCGACAGTATCTACGGGGTCCGCCGGCCCAGCGGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                              <  2:635878/139‑1 (MQ=17)
                                                            aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCCACGGGAGCTACCGGGGACGCCGGAGCCACCGGACCAACCGGAGCgaccggcgcgacaggagcga                                                                              >  2:3429716/1‑138 (MQ=17)
                                                            aCCAGCCGGACCGACGGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACCGGCGCtg                                                                              >  1:439391/1‑139 (MQ=17)
                                                               aGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAGCCGGACCAAGAGGAGCGACCGGAGCCACGGGAGCTACCGGCGACGCCGGACCAGCCGGACATACGGGCGCGTCAGGAGGGGCAGGAGCTAGCg                                                                           >  1:2794751/1‑140 (MQ=14)
                                                                gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGCAACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCgg                                                                          >  1:2211667/1‑140 (MQ=255)
                                                                gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                          <  2:3283573/140‑1 (MQ=255)
                                                                 ccGGAGCGCCGGGCGCGACCGGAGCCACCGGGGCCGCCGGACCAGCCGGCCCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                         <  1:4039851/140‑1 (MQ=14)
                                                                 ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                         <  1:4840591/140‑1 (MQ=255)
                                                                     aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                     <  2:192647/140‑1 (MQ=255)
                                                                     aGCGACGGGAGCGACAGGAGCGACCGGTCAAGCCGGAGCGGCCGGAGCAACCGGAGCTCGGGGTGACACCGGACCGCCCGCGCACGCCCGACCTTCCGCACCCCCCGGAGCGACAGGCGCTGCCGGAGCTTGCGGGGGAg                                                                     >  1:1290854/1‑140 (MQ=255)
                                                                          cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACCGGAGCGACCGGGGCCCCCGGAGCAACCGTGGCACCGGGACCACACGGAGCCCCCGGACCCACAGGAGCGACCGGGGCTCCCGGGGAAGCCGGa                                                                >  1:3884003/1‑140 (MQ=9)
                                                                           gggCGCGACAGGAGCGACCGGGGAAGCCGGCCCAGCCGCACCACCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                               <  2:2221276/140‑1 (MQ=17)
                                                                           gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACAGGAGCTACGGGAGCCACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCAGCCGGAGCGACCGGAGCGACGGGAGCGACAGGGGAGGCggggc                                                               >  1:444975/1‑138 (MQ=14)
                                                                               gCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                           <  2:4766812/140‑1 (MQ=255)
                                                                                ccacgggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGACCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                          <  1:2727883/135‑1 (MQ=14)
                                                                                cGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                          <  1:978829/140‑1 (MQ=255)
                                                                                cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACTGGAGCTACCGTGGACGCCTGACCAGCCCGACCAACCTCAGAGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                          >  1:4388650/1‑140 (MQ=17)
                                                                                cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                          <  1:2908043/140‑1 (MQ=255)
                                                                                cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                          <  1:3889758/140‑1 (MQ=255)
                                                                                cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                          <  1:3604743/140‑1 (MQ=255)
                                                                                        gctcgcGGGGAAGCCGGCCCACCCGGACCACCCGGTGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGTCAGGAGCGACAGGAGGTACCGGGGAAGCCGGACCAGCCggagcgac                                                  <  1:3832808/135‑1 (MQ=17)
                                                                                         cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                 >  2:137176/1‑108 (MQ=255)
                                                                                           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGCGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                               >  2:3507304/1‑140 (MQ=255)
                                                                                           aCCGGGGAAGCCGGACCAACCGGAGCAACCGGAGCCACGGGAGCCACGGGAGCACGCGGGCAAGCCGGAGCAGCAGGAGCTACCGGCGAGACAGGACCCACCGGAGCGCCAGGTGACCCGGGAGCAGCCggagcgggagg                                               >  1:1635788/1‑135 (MQ=2)
                                                                                            gcgacccagccggaccagccggaccAACCGGAGCTCCGGGAGCCACGGGAGCTACCGGCGAACCCGGAGCACCCGGTCCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCggagcgacggga                                              <  2:255590/133‑1 (MQ=14)
                                                                                                 gacgccggccccgccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                                         <  1:1229663/137‑1 (MQ=255)
                                                                                                    gccggaccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                      <  1:166335/140‑1 (MQ=255)
                                                                                                    gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                          <  2:1241587/120‑1 (MQ=255)
                                                                                                         accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                 >  1:3428424/1‑140 (MQ=255)
                                                                                                          gcagccggcccaCCCGGAGGGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                <  2:3225883/139‑1 (MQ=17)
                                                                                                          gcagccggaccaACCGGCCCTACGGGAGGCAGGGGAGCTACCGGTGAAGCCGCACCAGCCGGACCAACCGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                <  2:730427/139‑1 (MQ=17)
                                                                                                          ccagccggaccaCCCGGTGCGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCACCCGCACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                <  2:4397884/140‑1 (MQ=17)
                                                                                                             gccgccccaCCCGGAGCGACGGGAGCCAGGGGAGCTACCGGGGAAGCCGGCCCCGCCGGCCCAACCGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggcgcgacagg                             <  1:1479461/134‑1 (MQ=17)
                                                                                                              ccggaccaGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga                            >  2:2707092/1‑140 (MQ=255)
                                                                                                               cggaccaACCGGAGGGACGGGAGCCCCGTGATCTACAGGGGAATCCGGACCAGCCGGACCACCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag                           <  2:4191517/140‑1 (MQ=17)
                                                                                                                 gaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt                         <  1:558602/140‑1 (MQ=255)
                                                                                                                  acccACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCCGCGGGTCCCACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGAGCGACAGGTGCGACAGGAGCGACAGGAGCTa                        <  1:4939389/140‑1 (MQ=17)
                                                                                                                      aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg                    >  1:2635490/1‑140 (MQ=255)
                                                                                                                      aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgc                    >  1:730427/1‑139 (MQ=255)
                                                                                                                       ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCTACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                   <  2:513303/140‑1 (MQ=255)
                                                                                                                       ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                   >  2:3740338/1‑140 (MQ=255)
                                                                                                                           acccccgggagccacgggagccaCGGGGGACGCCGGACCAGCGGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAg               <  1:1856490/135‑1 (MQ=14)
                                                                                                                               ccGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCGACAGGAGCCACCGGAGCGACTGGGGCACCTGGCCCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTgg           <  2:336778/139‑1 (MQ=11)
                                                                                                                               aCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTgg           >  1:3364984/1‑140 (MQ=255)
                                                                                                                                cGGGAGCCCCCGGAGCCACCGGCGCTCCCGGACCAGCCGGACCTCCCGGAGCGACTGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa          <  2:3157693/140‑1 (MQ=14)
                                                                                                                                cGGGAGCCACGGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa          <  2:2099723/140‑1 (MQ=255)
                                                                                                                                cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa          <  1:3916382/140‑1 (MQ=255)
                                                                                                                                  ggAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGAGCGACAGGAGCGACCGGGGAAGCTGGagc        >  2:890244/1‑138 (MQ=17)
                                                                                                                                     gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACCGGAGCGACAGGAGCGACAGGACCAACCGGTGAAGCCGGAGCGTCAGGAGCGACAGGTGCGTCAGGAGCGGCAGGAGCGAGCGGCGCATCGGGagcagc     >  2:979773/1‑140 (MQ=9)
                                                                                                                                     gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                       <  2:4647056/42‑1 (MQ=255)
                                                                                                                                     gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                       >  1:4647056/1‑42 (MQ=255)
                                                                                                                                      ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt    >  1:205687/1‑140 (MQ=255)
                                                                                                                                      acACGGGAGCTCCCGCGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt    <  2:1857009/139‑1 (MQ=17)
                                                                                                                                        aCGGGAGCTACCGGCGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTgg  <  1:4785868/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
AGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGG  >  NZ_CP034943/607241‑607516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: