BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,413	A→C	9.5%	T86P (ACC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,413	0	A	C	9.5%	568.5 / 7.2	257	T86P (ACC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (102/115); new base C (6/31); total (110/147)
Fisher's exact test for biased strand distribution p-value = 4.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

GCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607275‑607552

gCCACGGGAGCTACCGGCGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                                                                            <  2:366903/140‑1 (MQ=255)

gCCACCGGAGCCCCCGGCGCAGCCGGACCAGAAGGAGCGCCGGGAGCCACGGGAGCTACCGGAGCTACCGGCCCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                                                                            <  2:1275947/140‑1 (MQ=11)

          cTACCGGGGAAGCTGGACCAGCCGGAGCGAGGGGAGCGAAAGGAGCTACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                                                                                  <  1:3258472/140‑1 (MQ=255)

          cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACCGGAGCGACAGGCGCAACGGGAGCTCCGGGATCCGCCGCAGCGGCCGGAGCAACCtgagcgcca                                                                                                                                  >  1:1763982/1‑140 (MQ=2)

          cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                                                                                  <  1:1504757/140‑1 (MQ=255)

              cGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                                                                              <  2:4418558/140‑1 (MQ=255)

                 ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGCACCACCCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACCggagcgacaggagcga                                                                                                                           >  1:2446485/1‑140 (MQ=14)

                  gAAGGTGCACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGTGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  2:2062081/140‑1 (MQ=17)

                  gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                                                                       >  1:5156070/1‑127 (MQ=255)

                  gAAGCTGGACCAGCCGGAGCGAAGTGACCGAAAGGAGCTAACTGTGAAGCGGGACCAGCCGTACCATCCGTAGCGAAGTGAGCCACGGGAGCTACCGGGGAAGCCGGAACAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  2:834156/140‑1 (MQ=18)

                    aGCTGGACCAGCCGGAGCGACGGGAGGGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAACAACCGgagcgacaggagcgacag                                                                                                                        <  1:4660911/140‑1 (MQ=255)

                    aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCggagcgacaggagcgaccg                                                                                                                        >  2:4513767/1‑138 (MQ=255)

                    aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                                                                        <  1:4789687/140‑1 (MQ=255)

                    aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGGCCACCCGgagcgacgggcgcgacag                                                                                                                        >  2:3175305/1‑140 (MQ=17)

                     gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       <  2:4913894/140‑1 (MQ=255)

                       tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCCACCGGAGCGACCGGCGCAACCGGCCACGCCGGCACAGCCCGACCAACCGgaccgagaggggcgagagcgc                                                                                                                     >  2:3999540/1‑137 (MQ=14)

                        ggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCgacaggagcgacaggagc                                                                                                                    <  1:4216657/140‑1 (MQ=255)

                        ggACCAGCCGGACCGCCGGGAGCGCCGGGAGAGACCGGCGCACCCGGAGCACCAGGACCACCCGGAGCCACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCgacaggagcgacaggagc                                                                                                                    <  1:197097/140‑1 (MQ=14)

                         gACCAGCCGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCt                                                                                                                   <  2:2668791/140‑1 (MQ=255)

                          aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGCGCTa                                                                                                                  >  1:4177968/1‑140 (MQ=255)

                          aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                  >  1:439298/1‑140 (MQ=255)

                              gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                              <  2:3283573/140‑1 (MQ=255)

                              gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGCAGCTCCCGGCGCAGCCGGACCAGCGCGAGCACGCGGTGCGACAGGAGGGACAGGAGCGGCCgg                                                                                                              >  1:4563665/1‑140 (MQ=14)

                               ccGGAGCGCCGGGCGCGACCGGAGCCACCGGGGCCGCCGGACCAGCCGGCCCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  1:4039851/140‑1 (MQ=14)

                               ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             >  2:1231725/1‑140 (MQ=255)

                                   accgcccggAGCCACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  2:4537321/133‑1 (MQ=14)

                                   aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  2:192647/140‑1 (MQ=255)

                                   aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:2700446/140‑1 (MQ=255)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGTAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    >  1:3106948/1‑140 (MQ=255)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCCGGCCCAGCCGGACCAACCGGAGCGACCGGAGCGACCGGCGCTCCCGGGGAAGCCGGa                                                                                                    >  1:3220840/1‑140 (MQ=17)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    >  1:2379277/1‑140 (MQ=255)

                                         gggCGCGACAGGAGCGACCGGGGAAGCCGGCCCAGCCGCACCACCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  2:2221276/140‑1 (MQ=17)

                                         gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGCCAGGAGCGACAGGAGCTCCCGGGGAAGCCGGAc                                                                                                   >  2:107359/1‑140 (MQ=255)

                                         gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACAGGAGCTACGGGAGCCACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCAGCCGGAGCGACCGGAGCGACGGGAGCGACAGGGGAGGCggggc                                                                                                   >  1:444975/1‑138 (MQ=14)

                                         gggAGCCAGCGGAGCTACCGGCGATTCCGGCCCAGCCGCACCACCCGGAGCGACGGGAGCCACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  2:876399/140‑1 (MQ=14)

                                             gCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                               <  2:4766812/140‑1 (MQ=255)

                                              ctaaaGGAGCAACCGGGCAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                              <  1:783720/136‑1 (MQ=17)

                                              cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                              <  1:3604743/140‑1 (MQ=255)

                                                      gctcgcGGGGAAGCCGGCCCACCCGGACCACCCGGTGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGTCAGGAGCGACAGGAGGTACCGGGGAAGCCGGACCAGCCggagcgac                                                                                      <  1:3832808/135‑1 (MQ=17)

                                                       cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                                                     <  1:137176/108‑1 (MQ=255)

                                                         aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGCGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                   >  2:3507304/1‑140 (MQ=255)

                                                         aCCGGGGAAGCCGGACCAACCGGAGCAACCGGAGCCACGGGAGCCACGGGAGCACGCGGGCAAGCCGGAGCAGCAGGAGCTACCGGCGAGACAGGACCCACCGGAGCGCCAGGTGACCCGGGAGCAGCCggagcgggagg                                                                                   >  1:1635788/1‑135 (MQ=2)

                                                          gcgacccagccggaccagccggaccAACCGGAGCTCCGGGAGCCACGGGAGCTACCGGCGAACCCGGAGCACCCGGTCCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCggagcgacggga                                                                                  <  2:255590/133‑1 (MQ=14)

                                                               gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                                                                             <  1:3856718/140‑1 (MQ=255)

                                                               gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAATCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCACCCGGAGCAGCCGGAGCAGCCggagcgacaggagcgac                                                                             >  1:2279277/1‑140 (MQ=17)

                                                               gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                                                                             <  1:281978/140‑1 (MQ=255)

                                                               gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                                                                             >  1:578306/1‑140 (MQ=255)

                                                                  gccggaccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          <  1:166335/140‑1 (MQ=255)

                                                                  gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          >  2:3123536/1‑140 (MQ=255)

                                                                       accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                                                     >  1:3885678/1‑140 (MQ=255)

                                                                        gcagccggcccaCCCGGAGGGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  2:3225883/139‑1 (MQ=17)

                                                                        cccgccggaccaCCCGGACCCCATGGAGCACCCGGACCTCCCGGGCCACCCGGACCAGCCGGACCCCCGGGAGTCACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:1858344/137‑1 (MQ=2)

                                                                        ccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  2:3428424/140‑1 (MQ=255)

                                                                           gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                 <  1:669178/140‑1 (MQ=255)

                                                                           gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                 <  1:3393659/140‑1 (MQ=255)

                                                                            ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacgggagccacaggcgcctcggga                                                                >  1:255590/1‑133 (MQ=14)

                                                                             cggaccaACCGGAGGGACGGGAGCCCCGTGATCTACAGGGGAATCCGGACCAGCCGGACCACCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag                                                               <  2:4191517/140‑1 (MQ=17)

                                                                              gcaccaACCGCAGCGACGGCAGCCACGGGCGCGACCGCGGAAGCCGGACCAGCCGGACCAACCGGAGCGCCAGGAGCGACACGAGCTACCGGGGAAGCCGGACCAGCGGGAGCgacaggagcgacaggagcgacaggagc                                                              <  1:908387/138‑1 (MQ=17)

                                                                                acccACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCCGCGGGTCCCACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGAGCGACAGGTGCGACAGGAGCGACAGGAGCTa                                                            <  1:4939389/140‑1 (MQ=17)

                                                                                accaACCGGAGCCACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa                                                            <  1:2148518/140‑1 (MQ=255)

                                                                                    aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGTCCGGAGCTACAGGGGTCGCCGGACCCGCCGGTGCGACAGGTGCGCCAGGCGCGCGAGGTGCTACCgg                                                        >  1:5166578/1‑140 (MQ=17)

                                                                                    aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgc                                                        >  1:3026279/1‑139 (MQ=255)

                                                                                     ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCTACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  2:513303/140‑1 (MQ=255)

                                                                                         aGCCACGGGAGCCACCGGAGCTACCGGCGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  2:4616835/140‑1 (MQ=14)

                                                                                             ccGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCGACAGGAGCCACCGGAGCGACTGGGGCACCTGGCCCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTgg                                               <  2:336778/139‑1 (MQ=11)

                                                                                             aCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAATCTgg                                               <  1:2171432/140‑1 (MQ=255)

                                                                                              cGGGAGCCTCGGGAGCTACGGGGGCAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  2:2215709/140‑1 (MQ=14)

                                                                                              cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  1:3916382/140‑1 (MQ=255)

                                                                                               gggAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGAGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAc                                             <  1:1231221/140‑1 (MQ=255)

                                                                                               gggAGCCACGGGAGCTACCGGCGAAGCCGGACCAGCCGGACCCCCCGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCTGGAc                                             <  2:38938/140‑1 (MQ=17)

                                                                                                ggAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGAGCGACAGGAGCGACCGGGGAAGCTGGagc                                            >  2:890244/1‑138 (MQ=17)

                                                                                                   gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACCGGAGCGACAGGAGCGACAGGACCAACCGGTGAAGCCGGAGCGTCAGGAGCGACAGGTGCGTCAGGAGCGGCAGGAGCGAGCGGCGCATCGGGagcagc                                         >  2:979773/1‑140 (MQ=9)

                                                                                                   gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                                                           <  2:716226/42‑1 (MQ=255)

                                                                                                   gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                   >  2:2813266/1‑98 (MQ=255)

                                                                                                    ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                        >  1:205687/1‑140 (MQ=255)

                                                                                                    ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                        >  1:553375/1‑140 (MQ=255)

                                                                                                    acACGGGAGCTCCCGCGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                        <  2:1857009/139‑1 (MQ=17)

                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGACCCGCTGGAGCGACg                               >  1:3764321/1‑140 (MQ=255)

                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:1279227/116‑1 (MQ=255)

                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:1241227/116‑1 (MQ=255)

                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  2:1241227/1‑116 (MQ=255)

                                                                                                               cccGGGGAAGCCGGACCTCCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACggg                             <  2:4940360/139‑1 (MQ=255)

                                                                                                               aCCTGGGATGCCGGCCCAGCCTGACCCACCGGAGCGACAGGAGCGACAGGCGCTACCGGTGAAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:166355/140‑1 (MQ=14)

                                                                                                               aCCGGGGACGCGGGACCCGCCGGACCAACCGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCGGGTGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  1:2580296/140‑1 (MQ=17)

                                                                                                               aCCGGGGAAGCCGCACCCGCCGGACCACCCGGAGCGACAGGAGCGCCCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:632242/140‑1 (MQ=14)

                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCACCCGCAGCGCATGTAGCGACAGGAGCTACCGGGGAAGCCGTACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  1:1200367/140‑1 (MQ=17)

                                                                                                                    ggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCTGTGAAGCCGGACCAGCCGGATCGACAGGAGCGACAGGAGCTACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGa                        >  1:2037739/1‑140 (MQ=255)

                                                                                                                    ggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGa                        >  1:4820800/1‑140 (MQ=255)

                                                                                                                        gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGTGCGACAGGAGCGACAGGAGCTCCCGGCGAAGCTGGCCCAGCTGGAGCGACGTGAGCGACCgg                    >  1:4297231/1‑140 (MQ=17)

                                                                                                                        cccggaccagccggaccaCCCGTAGCGACTGGAGTGACAGGTCCTACCGGGGTAGCCGGACCAGCCGGAGCCACAGCAGCGACTGTAGCGACAGGCGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                    <  2:439391/139‑1 (MQ=18)

                                                                                                                        cccggaccagcccgaccaACCCGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACTGGAGCGACAGGTGCGACACGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                    <  2:4177968/139‑1 (MQ=14)

                                                                                                                         ccggtgcagccggaccaCCCGGCGCGACCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGCCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGa                   <  2:5187336/134‑1 (MQ=14)

                                                                                                                           ggatcagccgtaccaACCGGAGCTACAGGAGCCACAGGAGCTCCCGGTGAAGCAGGACCAGCCGGAGCCACAGGAGCGACCGGAGCCACTGGAGCTACCGGGGATGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGc                 <  2:3837774/140‑1 (MQ=14)

                                                                                                                           ggaccacccggaccacccGGAGCCATAGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCAGGAGCGACGGGAGCGACAGGAGc                 <  2:1133910/140‑1 (MQ=11)

                                                                                                                             accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCAGGACCAGCGGGAGCGACGGGAGCCACAGGAGCTa               >  1:2768035/1‑140 (MQ=255)

                                                                                                                             accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGa                        >  2:157669/1‑131 (MQ=255)

                                                                                                                             accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCGGGAGCGACCGGAGCGACAGGAGcga               >  2:303960/1‑138 (MQ=255)

                                                                                                                                cgccggacccCCCCGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGCACCCACCGGCGCCACAGGAGCGACAGGAGCTACCGGGCAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCg            <  2:3106948/139‑1 (MQ=14)

                                                                                                                                agccggaccaCCCGCAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCg            <  2:2379277/140‑1 (MQ=17)

                                                                                                                                      accaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGAGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCggggccg      >  1:1070776/1‑137 (MQ=255)

GCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607275‑607552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`