BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,592	A→C	21.1%	E145D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,592	0	A	C	21.1%	412.2 / 103.7	267	E145D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (107/99); new base C (20/40); total (127/140)
Fisher's exact test for biased strand distribution p-value = 1.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

CCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACC  >  NZ_CP034943/607455‑607730

    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                                                      <  1:3304088/140‑1 (MQ=255)

    ccGGACCGACCGGAGCGCCAGGAGCGACACGAGCTCCCGGCGCACCTGGACCAGCTGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                                                      <  1:4143413/140‑1 (MQ=21)

     cggggaagccggacccaccggagcgaccggagccacaggggctactggggacggcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                                                                                                     >  1:1035173‑M1/107‑140 (MQ=255)

     cGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAc                                                                                                                                     <  1:3480966/140‑1 (MQ=255)

         gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                                                                                 <  1:3199332/140‑1 (MQ=255)

         gaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                                                                                                 <  1:2118006‑M1/38‑1 (MQ=255)

          cgacaggagcgacaggagcgacaggagCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                                                                <  1:3661952/140‑1 (MQ=255)

            ccctgtgcgacaggagcgacaggagcTACCGGGGAAGCTTGACCAGCTGGAGCTACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                                              <  2:1682561/134‑1 (MQ=255)

             caggcgcgacaggagcgacaggagcTCCCGGCGAAGCTGGACCACCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                                             <  1:890244/140‑1 (MQ=25)

             caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                                             <  1:106696/140‑1 (MQ=255)

             caggagccacaggagctaaaggcgcAAAAGGTGAACCTGGACCCCCTGGAGCCAGGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                                             <  1:2451885/140‑1 (MQ=18)

             caggaccgccaggagctaaaggagcTACAGGCCAACATGGACCAGCTGGAGCCACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                                             <  1:4240025/140‑1 (MQ=18)

              aggagcgacaggagcgccaggcgcGACCGGGGAAGCCGGACCACCTGGACCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                                                            <  2:1270449/140‑1 (MQ=21)

              aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                                              <  1:5126311/138‑1 (MQ=255)

              aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                                              >  2:3789380/1‑138 (MQ=255)

              aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                                                            >  1:1418692/1‑140 (MQ=255)

              aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                                                            >  1:5132606/1‑140 (MQ=255)

                     accggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                                                                                                     <  2:5097889‑M1/50‑1 (MQ=255)

                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACggg                                                                                                                     <  1:4085042/140‑1 (MQ=255)

                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                     <  1:3122189/140‑1 (MQ=255)

                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACGGGAGCCACAGGGGCAACTGGGGAAGCCGGACCAGCCTGAGCGACAgg                                                                                                                     >  2:2389028/1‑140 (MQ=255)

                      gcggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                                                                                                                    >  1:4688620‑M1/90‑140 (MQ=255)

                      ccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCAACTGGCGACGTCGGACCAGCCGGAGCGAcagtc                                                                                                                    <  1:2803115‑M1/51‑3 (MQ=255)

                       aggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGGGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                   >  2:4608365/1‑140 (MQ=255)

                          cgcgaccggagcTCCCGGCGCCGCTGGCCCCGCTGGAGCCCCGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  1:2446716/139‑1 (MQ=17)

                          agcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  2:658559‑M1/55‑1 (MQ=255)

                          agcgaccggagcTACCGGGGTAGCTGCAACAGAGGGAGCCACTGGAGCGACCGGAGCTACCGGGGAAGCCGGCCCATCCGGCGCCACGGGAGCCACTGGGGCTTCTGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  1:2648621/140‑1 (MQ=17)

                            cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACTGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                              >  1:1371127/1‑140 (MQ=255)

                               cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGACAGAAGCTAccgcg                                                                                                           <  1:4777709‑M1/60‑3 (MQ=255)

                                   agcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  1:5192766/140‑1 (MQ=255)

                                   agcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  1:4823793/140‑1 (MQ=255)

                                    gccacatgggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                                                                                                     <  2:5191966‑M1/50‑1 (MQ=255)

                                    gccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGTAAGc                                                                                                      <  2:3499365‑M1/65‑1 (MQ=255)

                                     cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTCCTGGGGACGACGGACCAGCCGGAGCGCCAGGAGCTACAGGGGAAGcc                                                                                                     >  1:3369853/1‑140 (MQ=18)

                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACTGGGGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCgg                                                                                                   >  2:5003887/1‑140 (MQ=255)

                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                   >  1:4635409/1‑140 (MQ=255)

                                         cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCAACAGGGGCTCCTGGGGAAGCCGGACCTGCCGGAGCGACAGGAGATACCGGGGAAGCCGGAc                                                                                                 >  2:5123540/1‑140 (MQ=18)

                                         cGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGATCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCGACAGGGGCTACGGGGGAAGCCGGACCGGCCGGAGCGACCGGTGCTGCCGGGGCCGCCGGAc                                                                                                 >  1:5027581/1‑140 (MQ=18)

                                             gctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAccaac                                                                                             <  1:3737904‑M1/74‑3 (MQ=255)

                                               aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                           >  2:1914794/1‑140 (MQ=255)

                                               aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                           >  2:4555062/1‑140 (MQ=255)

                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                          >  2:2780323/1‑140 (MQ=255)

                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                          <  1:2780323/140‑1 (MQ=255)

                                                actggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCggaccaacacg                                                                                          <  2:110952‑M1/77‑6 (MQ=255)

                                                  tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGAg                                                                                        >  1:2323441/1‑140 (MQ=255)

                                                  gggACCCGCTCGAGCGACGGGCGCCTCACGCCCTACCGGCGCTGCCGGACCGGCCGGGGCCACGGGTGGCACCGGGGCTTCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                        <  2:1070776/139‑1 (MQ=17)

                                                     aCCAGCGGGAGCGACGGGAGCGACAGGCGGTACCGGGGAAGCCGGCCCAGCCGGGGCCAGGGGAGCCCGTGGGGCGACTGGGGAAGCCGGAGCCGCCGGAGCGCCTGGCGCTGCCGGGGGAGCGGGCGCGGCGGGAGCgg                                                                                     >  1:1340369/1‑139 (MQ=16)

                                                      ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                    <  1:4570664/140‑1 (MQ=255)

                                                       ccgCTCGAGCGACGGGAGCGACAGGAGCGAGCGGGGAACCCGGACCACCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGGGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                   <  1:655751/138‑1 (MQ=14)

                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCGACGGGAGCCACAGGGGCTACTGGGGAAGACGGACCTGCCGGAGCGACAGGAGATACAGCGCAAGCCGGCCCAGCCTGAGCGAAAGGa                                                                                >  1:4210169/1‑140 (MQ=14)

                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACGGGGGCTCCCGGCGAAGCATCACCAACCGCAGCCACAGGCGCTACCGGGGAACCCGCACCGGCCGCAGCGACAgcc                                                                                >  1:4315486/1‑138 (MQ=17)

                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGCGCGACAGGa                                                                                >  2:1394829/1‑140 (MQ=255)

                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACgggc                                                                                >  1:4981866/1‑139 (MQ=255)

                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACCGGa                                                                                >  2:4070918/1‑140 (MQ=255)

                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGcca                                                                            >  2:215638/1‑138 (MQ=255)

                                                                  aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                        >  1:1667646/1‑140 (MQ=255)

                                                                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                       >  1:4882730/1‑140 (MQ=255)

                                                                   cGGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGCGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                       <  1:1788769/140‑1 (MQ=255)

                                                                       aGCGACAGGAGCGACCGGGGCCGCCGGACCCGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                   <  1:1320649/140‑1 (MQ=17)

                                                                            cAGGAGCTACCGGGGCCGCCGGACCCGCCGGGGCCACGGCAGCCACAGGCGCTACTGGTGAAGCCGGAGCAGCCGGAGCGACAGGTGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                              <  2:2270518/140‑1 (MQ=18)

                                                                            cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                              <  2:338847/140‑1 (MQ=255)

                                                                             aGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                             <  2:4235051/140‑1 (MQ=255)

                                                                             aGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                             <  1:1400060/140‑1 (MQ=255)

                                                                                    aCCGGGGAAGCCGGACCAGCCGGGGCAACGGGAGCCCCAGGGGCTACTGGGGAAGCCGGACCACCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                      <  1:707918/140‑1 (MQ=255)

                                                                                    aCCGGGGAACCCGGACCAGCCGGCGCCACGGGAGCCACCGGCGCTACAGGGGAACCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                      <  2:2635490/140‑1 (MQ=17)

                                                                                          gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                >  1:34131/1‑140 (MQ=255)

                                                                                           cccccGGACCAGCCGGGGCCACGCCAGCCACAGGGGCGACGGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                               <  2:2037956/137‑1 (MQ=17)

                                                                                           caGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAATCCGGACCAGCCGGTGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                               <  2:4820800/139‑1 (MQ=255)

                                                                                             gCCGGACCAGCCGGCGCCACGGGAGCCACAGGGCCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGTAGCGCCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAgg                                             <  1:978925/140‑1 (MQ=17)

                                                                                              ccGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGa                                            >  1:1588301/1‑140 (MQ=255)

                                                                                                  accaccCGGGGCCACGGGAGCCACAGGCGCGACTGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                        <  1:2920447/140‑1 (MQ=17)

                                                                                                       ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       >  2:1480447/1‑72 (MQ=255)

                                                                                                       ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  1:1480447/72‑1 (MQ=255)

                                                                                                           ggCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGAc                                                                                                                        >  1:3569081/1‑51 (MQ=255)

                                                                                                           ggCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGAc                                                                                                                        <  2:3569081/51‑1 (MQ=255)

                                                                                                           ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAc                                                                                                                                     <  2:1131841/38‑1 (MQ=255)

                                                                                                           ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACGGGGGACGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAg                               >  1:3040525/1‑140 (MQ=17)

                                                                                                           ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                               <  1:2201263/140‑1 (MQ=255)

                                                                                                            gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGc                              >  1:4544933/1‑140 (MQ=255)

                                                                                                            gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGagcgaccggagcgaccggcgcacc                              >  2:1839586/1‑134 (MQ=14)

                                                                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                 >  1:3058891/1‑68 (MQ=40)

                                                                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGAc                         >  1:1234378/1‑140 (MQ=255)

                                                                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                         >  2:3174148/1‑140 (MQ=255)

                                                                                                                        tgagcagCTACCGGACAACCCGGACCCCACGGAGCCACAGGAGCAACCGGACAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                  <  2:4752670/134‑1 (MQ=11)

                                                                                                                        acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                            >  1:1942484/1‑66 (MQ=255)

                                                                                                                        acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                            <  2:75201/66‑1 (MQ=25)

                                                                                                                        acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                            >  1:75201/1‑66 (MQ=25)

                                                                                                                        acaGGGGCTACCGGCGAAGCCGGACCACCCGGAGCTACAGGCGCTACCGGGGAAGCCGGAGCACCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                  <  2:4160027/140‑1 (MQ=11)

                                                                                                                             ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                           >  1:3934734/1‑46 (MQ=40)

                                                                                                                              gCTCCTGGGGAAGCCGGAGCAGCCGGAGCGACTGGAGCTCCCGGTGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc            <  1:3767787/140‑1 (MQ=14)

                                                                                                                              gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGAAGCCGGACCAACCGGAGCCACTGGAGCGAACGGGGAAGACGCACAAGACGGAGctgc            >  2:1377381/1‑137 (MQ=11)

                                                                                                                              gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc            <  2:1333908/140‑1 (MQ=21)

                                                                                                                                 aCTGGTGAAGCCGCTCCCCCCGCCGCGACAGGAGCTTCCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAgg         <  2:4782150/140‑1 (MQ=14)

                                                                                                                                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCCGGAGCGACCGGCGCGGCCTGACCAGCCGGAGCGACCgg         >  2:3854169/1‑140 (MQ=14)

                                                                                                                                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg         <  2:2650447/140‑1 (MQ=255)

                                                                                                                                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGCAGCCGGAGAAGCCGGAGCGACGGCAGcga    >  2:3653110/1‑138 (MQ=11)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc   >  2:3520413/1‑140 (MQ=255)

                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                             >  2:194833/1‑81 (MQ=33)

                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                             <  1:194867/81‑1 (MQ=33)

                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCCACCGGAGCTAcc  >  2:244271/1‑140 (MQ=255)

CCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACC  >  NZ_CP034943/607455‑607730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`