Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,594 C→T 17.7% A146V (GCC→GTC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5940CT17.7% 541.5 / 117.3 283A146V (GCC→GTC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base C (128/100);  new base T (9/39);  total (142/141)
Fisher's exact test for biased strand distribution p-value = 2.27e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

CCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607455‑607732
                                                                                                                                           |                                                                                                                                          
ccAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGc                                                                                                                                            <  1:3047315/140‑1 (MQ=255)
   gCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCgg                                                                                                                                         >  2:3815699/1‑140 (MQ=255)
    ccggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                                                                                                        <  2:122270‑M1/33‑1 (MQ=255)
    ccGGCGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGa                                                                                                                                        <  1:1402820/140‑1 (MQ=255)
    ccGGAGCGACAGGAGCGACAGGAGCGACAGGCGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGCGACGCCGGAGCAGCGGGGGCCACGGGAGCCACAGGGGCGCCTGGGGCCGCCggc                                                                                                                                        >  2:109546/1‑139 (MQ=21)
    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                                                        <  1:2793622/140‑1 (MQ=255)
     cggggaagccggacccaccggagcgaccggagccacaggggctactggggacggcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                                                                                                       >  1:1035173‑M1/107‑140 (MQ=255)
         gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                                                                                   <  1:3199332/140‑1 (MQ=255)
         gaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGc                                                                                                                                   <  1:1852851‑M1/38‑1 (MQ=255)
            gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCgg                                                                                                                                <  1:3476753‑M1/41‑1 (MQ=255)
            gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                                                                                                                <  1:3001442‑M1/41‑1 (MQ=255)
            gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                                                                                                                <  1:288997‑M1/41‑1 (MQ=255)
            acaggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                                                <  2:1682806/140‑1 (MQ=255)
             caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                                               <  1:106696/140‑1 (MQ=255)
             caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                                               <  1:3740338/140‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  2:4811918‑M1/43‑1 (MQ=255)
              cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACCGGAGCCACAGGGGCTACAGGGGACGTCGGACCAGCCGGAg                                                                                                                              <  2:4293362‑M1/43‑1 (MQ=255)
              aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                                                              >  1:1418692/1‑140 (MQ=255)
                 cgcgaccggagcgacagcaccGACCGCGGAACCCGGACCCGCTGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                           <  1:4680724/139‑1 (MQ=18)
                  gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCGGGAGCGACCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCAGCgcgagcgag                                                                                                                          >  2:464486/1‑139 (MQ=21)
                     accggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACCGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                                                                                                       <  2:4767450‑M1/50‑1 (MQ=255)
                     accggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                                                                                                       <  2:5097972‑M1/50‑1 (MQ=255)
                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGGGCCAgg                                                                                                                       >  2:3525706/1‑140 (MQ=255)
                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCACCGGGCGCAGCCGGACCACCCGGAGCCACCgg                                                                                                                       >  2:578085/1‑140 (MQ=18)
                       cggagcgaccggagccacaggcgctactggggaagtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                                                                                                     >  2:702927‑M1/89‑140 (MQ=255)
                          agcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  <  2:101534‑M1/55‑1 (MQ=255)
                              acgggagccacatggtctactggggaagtcggtccagccggtgcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAGCgg                                                                                                              >  2:4176339‑M1/82‑140 (MQ=255)
                              acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGGCGGAGCGACAGGAGCGACCgg                                                                                                              >  1:3841732/1‑140 (MQ=255)
                              acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                              >  2:4570664/1‑140 (MQ=255)
                                   agcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:4866263/140‑1 (MQ=255)
                                     cTACCGGGGAAGCAGGACCAGCAGGAGCGACGGGAGCGACCGGAGCGACAGGGGAAGCCGGACCAGCCGGGGCCCCGGGAGCCACAGGGGCTCCGGTCGACGCCGGACCAGCCGGACCGCCAGGAGCTACCGGCGACGcc                                                                                                       >  1:978021/1‑140 (MQ=18)
                                         cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACCGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   >  1:4471913/1‑140 (MQ=255)
                                             gctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAccaac                                                                                               <  1:264176‑M1/74‑3 (MQ=255)
                                             gctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAccaac                                                                                               <  2:2393103‑M1/74‑3 (MQ=255)
                                             gctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGCACCAGCCTGAGCCACAGGAGCTACCGGGGAAgccggaacaac                                                                                               <  2:2279205‑M1/74‑6 (MQ=255)
                                              ctactggggacgtcggaccagccggagcgacaggagctaccggggaagcctgaccaaccggagcgACGGGAGCCACAGGGGCTACAGGGGACGTCGGACCAGCCGGAGCCACAGGAGCGACCGGCGAAGCCGGaccaacc                                                                                              <  2:2650509‑M1/75‑4 (MQ=255)
                                              ctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGTAAGCCGGaccaacc                                                                                              <  2:265038‑M1/75‑4 (MQ=255)
                                               aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGGGCCACCGGAGCCACCGGAGCAACAGGCCCAGGCGCAGCAGCCGGATCGACAGCAGCGCCGGGCGACGCCGGTCCAGGCg                                                                                             >  2:1666990/1‑140 (MQ=9)
                                                gCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACCCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                            <  1:3721471/140‑1 (MQ=255)
                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                            <  1:4693744/140‑1 (MQ=255)
                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACGTGAGCGACAGGGGCTACTGCGGAAGCCGCACCAGCCGCAGCCCCAGGAGCTAGCGGGGAAGCCGGACCTCCCgg                                                                                            >  1:3263388/1‑140 (MQ=17)
                                                  tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                          >  1:4235051/1‑140 (MQ=255)
                                                     aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGa                                                                                       >  1:4312213/1‑140 (MQ=255)
                                                     aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGa                                                                                       >  1:3599074/1‑140 (MQ=255)
                                                     aCCAGCGGGAGCGACGGGAGCGACAGGCGGTACCGGGGAAGCCGGCCCAGCCGGGGCCAGGGGAGCCCGTGGGGCGACTGGGGAAGCCGGAGCCGCCGGAGCGCCTGGCGCTGCCGGGGGAGCGGGCGCGGCGGGAGCgg                                                                                       >  1:1340369/1‑139 (MQ=16)
                                                      ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                      <  1:4555062/140‑1 (MQ=255)
                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCGACGGGAGCCACAGGGGCTACTGGGGAAGACGGACCTGCCGGAGCGACAGGAGATACAGCGCAAGCCGGCCCAGCCTGAGCGAAAGGa                                                                                  >  1:4210169/1‑140 (MQ=14)
                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGGCGGACCAGCCGGAGCGCGATGAGAAAGCGGGGAATCCCGACCCAGCGGAGCGACAGGa                                                                                  >  2:1126584/1‑140 (MQ=14)
                                                           tGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                 <  1:4119435/140‑1 (MQ=255)
                                                           gggAGCGACGGGAGCCACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                 <  1:1741047/139‑1 (MQ=255)
                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                              >  2:215645/1‑140 (MQ=255)
                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                              >  1:2650447/1‑140 (MQ=255)
                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGCCAGCAGCtc                                                                              >  1:4782150/1‑139 (MQ=255)
                                                                  aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                          >  1:1594459/1‑140 (MQ=255)
                                                                  aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                          >  1:5130457/1‑140 (MQ=255)
                                                                   ccggACCGACCGGAGCGACCGGGGAAGCCGGCCCAGCCGGGGCCCCGGGAGCCCCCGGGGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                         <  2:1932914/138‑1 (MQ=17)
                                                                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCGGCCGGAGCGACAGGAGCGACCggg                                                                         >  1:3337061/1‑140 (MQ=255)
                                                                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCGTGGGGACGCCTCACCACCCGCACCGCCCGCAGCTACCGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCGACCggg                                                                         >  1:1384797/1‑140 (MQ=14)
                                                                       aGCGACAGGAGCGACCGGGGCCGCCGGACCCGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                     <  1:1320649/140‑1 (MQ=17)
                                                                            cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                <  2:338847/140‑1 (MQ=255)
                                                                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                        >  1:338847/1‑140 (MQ=255)
                                                                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                        <  1:4969847/140‑1 (MQ=255)
                                                                                      cGGGGCAGGCGGACCCGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                      <  2:1300651/140‑1 (MQ=17)
                                                                                         ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCCACCGGCGACGCGGGACCAAACGGAGCGa                                                   >  1:2233033/1‑140 (MQ=17)
                                                                                          gatgCCGGACCACCCGGGGCCACGGGAGCCACAGGGGATACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTTCCGGGGAAGCCGGTCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                  <  2:5132606/137‑1 (MQ=17)
                                                                                           cccccGGACCAGCCGGGGCCACGCCAGCCACAGGGGCGACGGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                 <  2:2037956/137‑1 (MQ=17)
                                                                                             gCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCCGCCGGAGCTACAGTAGCTACCCGCGACGCCGGAGCTGCCGGAGGGACAGGAGCTACGGTGGAAGCCGGACCCCCCGGTGCgccggg                                               >  1:750141/1‑135 (MQ=255)
                                                                                             gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGCCCAGGCGCAGCggcggg                                               >  2:4118475/1‑135 (MQ=17)
                                                                                             gCCGGACCAGCCGGCGCCACGGGAGCCACCGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                               <  1:888695/140‑1 (MQ=255)
                                                                                             cccGGACCCCCAGGCGCCTCCGGAGCCCCAGGCCCTACGGGTGAACCTGGAGCACACGGAGCCACAGGACCTACCGGGGAAGCCGGACCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                               <  2:3499388/139‑1 (MQ=2)
                                                                                              ccGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCCTAGCGACACGCGATACCGGGTAACCCGGAGCAACCGGAGCGACCGGa                                              >  1:2367367/1‑140 (MQ=18)
                                                                                              ccGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGCGGCCGGCCCACCCGGAGCGACAGGa                                              >  1:195598/1‑140 (MQ=17)
                                                                                               cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                         <  1:4589938/112‑1 (MQ=255)
                                                                                                       ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         >  2:5009696/1‑72 (MQ=255)
                                                                                                       ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:1480447/72‑1 (MQ=255)
                                                                                                       ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:5009696/72‑1 (MQ=255)
                                                                                                           ggCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                 <  1:603801/140‑1 (MQ=255)
                                                                                                           ggCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGAc                                                                                                                          <  2:3569081/51‑1 (MQ=255)
                                                                                                           ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                 <  1:668329/140‑1 (MQ=255)
                                                                                                            gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                         <  2:1451529/99‑1 (MQ=255)
                                                                                                                 gggAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGACCAGGCGGAGCGTCAGGAGCTACCGGCGAAGCCGGAGCAACCGGAGCGACAGGAGCGACCGGGGAATCGGGAc                           >  1:3098905/1‑140 (MQ=14)
                                                                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                                 >  1:3534112/1‑54 (MQ=255)
                                                                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACGGGGGAAACAGGAc                           >  2:4995255/1‑140 (MQ=14)
                                                                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGAc                           >  1:1234378/1‑140 (MQ=255)
                                                                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                           >  2:3174148/1‑140 (MQ=255)
                                                                                                                             ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             <  2:3934734/46‑1 (MQ=40)
                                                                                                                              gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGAAGCCGGACCAACCGGAGCCACTGGAGCGAACGGGGAAGACGCACAAGACGGAGctgc              >  2:1377381/1‑137 (MQ=11)
                                                                                                                                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                        <  2:2559464/95‑1 (MQ=37)
                                                                                                                                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGTCAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACAgg           >  1:2448624/1‑140 (MQ=255)
                                                                                                                                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAAACGGGGCATCAGGACCAACCGGAGCGACAGGAGCAAACGGCGAGCCCGGACCAGCCGGAGCGAGAgg           >  1:2145846/1‑140 (MQ=14)
                                                                                                                                   gtgcgAAGCCGCACCAGCCGCACCGCCCGGCGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg         <  1:3999540/136‑1 (MQ=11)
                                                                                                                                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGCAGCCGGAGAAGCCGGAGCGACGGCAGcga      >  2:3653110/1‑138 (MQ=11)
                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc     >  2:1865880/1‑140 (MQ=255)
                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                               <  1:194833/81‑1 (MQ=33)
                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                               >  2:194833/1‑81 (MQ=33)
                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCACCCGGAGCGACACGAGCTAcg    >  2:1388862/1‑139 (MQ=255)
                                                                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCCACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTAcc    >  1:517180/1‑140 (MQ=255)
                                                                                                                                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGCACCAACCGGAGCGACAGGAGCTAACCGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg  >  1:5040661/1‑140 (MQ=14)
                                                                                                                                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGACGCCGGAACAACCGGAGCGACCGGAGCGAACGGCGACGCCGGAACACCCCGAGCGACCTGAGCGACCgg  >  2:814536/1‑140 (MQ=11)
                                                                                                                                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAACAGGACCAACCGGAGCGTCCGGAGCAACAGGCGCATCCGGAAAAGCCGTAGCACCAGGAGCTAcagc  >  2:281745/1‑137 (MQ=11)
                                                                                                                                           |                                                                                                                                          
CCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607455‑607732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: