BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,638	G→C	10.0%	A161P (GCC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,638	0	G	C	10.0%	524.9 / 7.2	239	A161P (GCC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (117/86); new base C (9/17); total (132/107)
Fisher's exact test for biased strand distribution p-value = 3.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

AGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607502‑607777

aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                                                                          >  2:4555062/1‑140 (MQ=255)

 gCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACCCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                         <  1:3721471/140‑1 (MQ=255)

 gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                         <  1:4693744/140‑1 (MQ=255)

 gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                         <  1:2780323/140‑1 (MQ=255)

  cTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                                                        >  2:810353/1‑140 (MQ=255)

   tggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgAAGGGAGCCACAGGGGCTACTGGGGACGTCGCACCAGCCGGAGCGACAGGAGCTACCGGCTAAGCCTGACAAGCCGGAg                                                                                                                                       <  2:1378191‑M1/79‑1 (MQ=255)

   tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                                       >  1:4235051/1‑140 (MQ=255)

   gggACCCGCTCGAGCGACGGGCGCCTCACGCCCTACCGGCGCTGCCGGACCGGCCGGGGCCACGGGTGGCACCGGGGCTTCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                                       <  2:1070776/139‑1 (MQ=17)

      aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                                    >  2:3228194/1‑140 (MQ=255)

      aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAGCCGCAGCGa                                                                                                                                    >  2:3514727/1‑140 (MQ=255)

      aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                                    >  1:4844575/1‑140 (MQ=255)

      aCCAGCGGGAGCGACGGGAGCGACAGGCGGTACCGGGGAAGCCGGCCCAGCCGGGGCCAGGGGAGCCCGTGGGGCGACTGGGGAAGCCGGAGCCGCCGGAGCGCCTGGCGCTGCCGGGGGAGCGGGCGCGGCGGGAGCgg                                                                                                                                    >  1:1340369/1‑139 (MQ=16)

       ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCCCAGGGGCTACAGGGGACGCCGGACCCGCCGGCCCGACAGGAGCTACCGGGGCAGCCGGACCCCCCGGAGCGAc                                                                                                                                   >  2:1892134/1‑140 (MQ=17)

       ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                   <  1:4570664/140‑1 (MQ=255)

       ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                   <  1:1914794/140‑1 (MQ=255)

               aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                           >  2:215645/1‑140 (MQ=255)

               aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                           >  1:2650447/1‑140 (MQ=255)

               aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGCCAGCAGCtc                                                                                                                           >  1:4782150/1‑139 (MQ=255)

                   aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                                       >  1:1594459/1‑140 (MQ=255)

                   aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                                       >  1:1667646/1‑140 (MQ=255)

                    cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCGGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  1:3991440/1‑140 (MQ=255)

                    cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      <  1:810353/140‑1 (MQ=255)

                    cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  1:357814/1‑140 (MQ=255)

                    cGGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGCGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      <  1:1788769/140‑1 (MQ=255)

                        cccGACAGGAGCCACCGGCGAAGCCGGACCCCCCGGGGCCCCGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                  <  1:20894/138‑1 (MQ=17)

                        aGCGACAGGAGCGACCGGGGCCGCCGGACCCGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                  <  1:1320649/140‑1 (MQ=17)

                               ggcgcgcccGGGGACGCCGGAGCAGCCGGGGCCTCGGGAGCCCCCGGGGCTACTGTGGAAGCCGGACCTGCCGGCGCGACTGGAGGTACCGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                                                                                                           <  2:553375/133‑1 (MQ=18)

                                     aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                     <  1:4969847/140‑1 (MQ=255)

                                          ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCCACCGGCGACGCGGGACCAAACGGAGCGa                                                                                                >  1:2233033/1‑140 (MQ=17)

                                           gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                               <  2:1418692/140‑1 (MQ=255)

                                              gCCGGACCCGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                            <  1:5110052/140‑1 (MQ=255)

                                              gCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCCGCCGGAGCTACAGTAGCTACCCGCGACGCCGGAGCTGCCGGAGGGACAGGAGCTACGGTGGAAGCCGGACCCCCCGGTGCgccggg                                                                                            >  1:750141/1‑135 (MQ=255)

                                              gCCGGACCAGCCGGCGCCACGGGAGCCACAGGGCCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGTAGCGCCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAgg                                                                                            <  1:978925/140‑1 (MQ=17)

                                              gCCGCACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCCGGGGAAGGCGGACCTGCGGGAGCGTCAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAgg                                                                                            <  1:3408282/140‑1 (MQ=17)

                                                cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      <  1:105749/112‑1 (MQ=255)

                                                cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      <  1:4589938/112‑1 (MQ=255)

                                                cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  2:4589938/1‑112 (MQ=255)

                                                   aCCAGCCGGGGCCACGGCAGCCACAGGGGCGACTGGGGAAGCCGGACCCGCCGGCGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                                                                       <  2:2067195/140‑1 (MQ=17)

                                                        ccGGCGCCACGGGAGCCACAGGGGCGACGGGGGAAGCGGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                  <  1:1525193/140‑1 (MQ=14)

                                                            ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                              <  1:668329/140‑1 (MQ=255)

                                                            ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACGGGGGACGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAg                                                                              >  1:3040525/1‑140 (MQ=17)

                                                            ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                              <  1:2201263/140‑1 (MQ=255)

                                                             gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGCGAAGCCGGCCCAACCGGAGCCACCGGAGCTACCggcgcgcc                                                                             >  2:3592555/1‑134 (MQ=14)

                                                             gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGc                                                                             >  1:4544933/1‑140 (MQ=255)

                                                             gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  1:1451529/1‑99 (MQ=255)

                                                             gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGagcgaccggagcgaccggcgcacc                                                                             >  2:1839586/1‑134 (MQ=14)

                                                                  gggAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGACCAGGCGGAGCGTCAGGAGCTACCGGCGAAGCCGGAGCAACCGGAGCGACAGGAGCGACCGGGGAATCGGGAc                                                                        >  1:3098905/1‑140 (MQ=14)

                                                                  gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACGGGGGAAACAGGAc                                                                        >  2:4995255/1‑140 (MQ=14)

                                                                  gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGAc                                                                        >  1:1234378/1‑140 (MQ=255)

                                                                  gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGAAGCAGGAc                                                                        >  1:4987749/1‑140 (MQ=255)

                                                                         tgagcagCTACCGGACAACCCGGACCCCACGGAGCCACAGGAGCAACCGGACAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                 <  2:4752670/134‑1 (MQ=11)

                                                                         acaGGGGCTACCGGCGAAGCCGGACCACCCGGAGCTACAGGCGCTACCGGGGAAGCCGGAGCACCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                 <  2:4160027/140‑1 (MQ=11)

                                                                                cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACa                                                          >  2:4800763/1‑140 (MQ=255)

                                                                                  ccTGCGGCAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGTGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                        <  1:3279374/139‑1 (MQ=14)

                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGTCAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACAgg                                                        >  1:2448624/1‑140 (MQ=255)

                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCCGGAGCGACCGGCGCGGCCTGACCAGCCGGAGCGACCgg                                                        >  2:3854169/1‑140 (MQ=14)

                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                        >  1:605178/1‑140 (MQ=255)

                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                        <  2:2650447/140‑1 (MQ=255)

                                                                                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGcgc                                                   >  2:3021299/1‑138 (MQ=14)

                                                                                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGCAGCCGGAGAAGCCGGAGCGACGGCAGcga                                                   >  2:3653110/1‑138 (MQ=11)

                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGCCGCGACAGGAGCGAc                                                  >  2:1941028/1‑140 (MQ=11)

                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACGGGAGCTACAGGGGAAGCCGGAGACACCggagcgaccggagcgacc                                                 >  2:259950/1‑140 (MQ=11)

                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                            <  1:194867/81‑1 (MQ=33)

                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                            >  2:194867/1‑81 (MQ=33)

                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCACCCGGAGCGACACGAGCTAcg                                                 >  2:1388862/1‑139 (MQ=255)

                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCCACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                 >  1:517180/1‑140 (MQ=255)

                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCACCCGGAGCGACCGGAGCGACCGCGGACGCCGGACCAGCcggagcgaccggagcgaca                                                 >  1:1388170/1‑139 (MQ=11)

                                                                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCTACAGGGGAAGCCGGCCCAGCCGGAGCGACATGAGCTAcccg                                               >  1:4522301/1‑138 (MQ=14)

                                                                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                               >  2:160636/1‑140 (MQ=255)

                                                                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                               >  1:3225379/1‑140 (MQ=255)

                                                                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGCACCAACCGGAGCGACAGGAGCTAACCGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                               >  1:5040661/1‑140 (MQ=14)

                                                                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAACAGGACCAACCGGAGCGTCCGGAGCAACAGGCGCATCCGGAAAAGCCGTAGCACCAGGAGCTAcagc                                               >  2:281745/1‑137 (MQ=11)

                                                                                             cGGACCAGCCGGCGCGCCCGGAGCGACCGGGGACGCCGGCCCAGCCGGCGCCACAGGAGCTACCGGGGACGCCGGACCAACCGCAGCGTCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                             <  2:2470167/140‑1 (MQ=14)

                                                                                             cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACCCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                             >  2:5077943/1‑140 (MQ=255)

                                                                                             cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAACCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                             >  2:3097514/1‑140 (MQ=255)

                                                                                             cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGCGCCGCCGGACCAGCCGGAGCGACAGGAGCTCCCgggg                                             >  2:2227764/1‑140 (MQ=14)

                                                                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGAAGCGACAGGAGCTCCCGGGGAAg                                          >  2:1302273/1‑140 (MQ=255)

                                                                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAg                                          >  2:4018945/1‑140 (MQ=255)

                                                                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAACCGGAGCTACAGGAGCGACCGGGGAAGCCGGACAGTCCGGAGCGACAGGAGCGACCGGGGCAg                                          >  2:1248204/1‑140 (MQ=14)

                                                                                                      cGGAGCGACCGGCGCTACCGGTGCAGCCGGACCCCCCGGAGCTACCGGTGATACCGGGGAAGCCGGACCCACCGGAGCCACAGGCGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                    <  2:1234378/140‑1 (MQ=14)

                                                                                                      cGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGAc                                    <  1:3392087/140‑1 (MQ=255)

                                                                                                      cGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                    <  1:3520413/140‑1 (MQ=255)

                                                                                                       ggAGCGACTGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                                   <  1:3416512/140‑1 (MQ=255)

                                                                                                         aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGCCCAGACGGAGCGACAGGAGCTACCGCGGAAGCCGGACCac                                 >  2:4331260/1‑139 (MQ=14)

                                                                                                         aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGTAGCTACCGGGGACGCCGGACCaa                                 >  2:4494710/1‑140 (MQ=255)

                                                                                                          gccaccggAGCTACCGGGGCACGCGGACGAGCCGGAGCGACAGGAGCAACCTGTGATGCGGGAGGAAACGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGGGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                <  1:4118662/134‑1 (MQ=14)

                                                                                                             aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGCAGCCGGACCACCCgg                             >  1:4188194/1‑140 (MQ=255)

                                                                                                              cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGCACCAACCGGa                            >  2:2441624/1‑140 (MQ=255)

                                                                                                                   gCTACCGGGGAATCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGTAGCTACGGGCGACTCCGGACCAGCCGGAGCGACAGGAGCTACCTGGGAAGCCGGACCACACGGAGCGAc                       >  1:1046431/1‑140 (MQ=11)

                                                                                                                    cgcccGGGGAACCCGGCCCCCCCGCAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                      <  2:3337061/137‑1 (MQ=14)

                                                                                                                      cccGGGGCACCCGGACCACCCGGAGCTACAGGAGCTACCGGCGAACCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                    <  1:2047193/139‑1 (MQ=14)

                                                                                                                           ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGccc               >  1:4254827/1‑139 (MQ=255)

                                                                                                                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  2:356801/140‑1 (MQ=255)

                                                                                                                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                            >  1:4036765/1‑59 (MQ=33)

                                                                                                                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  2:485297/140‑1 (MQ=255)

                                                                                                                                ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  1:757743/136‑1 (MQ=255)

                                                                                                                                ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              >  2:757743/1‑136 (MQ=255)

                                                                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa      >  2:687139/1‑140 (MQ=255)

                                                                                                                                        gCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg  <  2:34131/140‑1 (MQ=255)

                                                                                                                                        gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCGACggg  >  2:1674198/1‑140 (MQ=255)

AGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607502‑607777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`