BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,699	A→C	5.3%	E181A (GAA→GCA) ‡	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,699	0	A	C	5.3%	549.1 / 3.1	219	E181A (GAA→GCA) ‡	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (115/85); new base C (15/3); total (130/89)
Fisher's exact test for biased strand distribution p-value = 4.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

GGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCG  >  NZ_CP034943/607562‑607835

ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACGGGGGACGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAg                                                                                                                                        >  1:3040525/1‑140 (MQ=17)

ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                        <  1:2201263/140‑1 (MQ=255)

 gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGc                                                                                                                                       >  1:4544933/1‑140 (MQ=255)

      gggAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGACCAGGCGGAGCGTCAGGAGCTACCGGCGAAGCCGGAGCAACCGGAGCGACAGGAGCGACCGGGGAATCGGGAc                                                                                                                                  >  1:3098905/1‑140 (MQ=14)

      gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGAAGCAGGAc                                                                                                                                  >  1:4987749/1‑140 (MQ=255)

      gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGCCCAACCGGAGCGACAGGAGCTCCCGGGGACGCCGGAc                                                                                                                                  >  1:4108744/1‑140 (MQ=14)

             acaGGGGCTACCGGCGAAGCCGGACCACCCGGAGCTACAGGCGCTACCGGGGAAGCCGGAGCACCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                           <  2:4160027/140‑1 (MQ=11)

                   gCTCCTGGGGAAGCCGGAGCAGCCGGAGCGACTGGAGCTCCCGGTGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                     <  1:3767787/140‑1 (MQ=14)

                   gCTACTGGGCATGCCGGAGGCGCCGGAGCTACAGGAGCTACCGGGGAATCCGGAGCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                     <  2:5091103/140‑1 (MQ=11)

                   gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                     <  2:1333908/140‑1 (MQ=21)

                      aCTGGTGAAGCCGCTCCCCCCGCCGCGACAGGAGCTTCCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAgg                                                                                                                  <  2:4782150/140‑1 (MQ=14)

                      aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCCGGAGCGACCGGCGCGGCCTGACCAGCCGGAGCGACCgg                                                                                                                  >  2:3854169/1‑140 (MQ=14)

                      aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                  >  1:605178/1‑140 (MQ=255)

                      aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                  <  2:2650447/140‑1 (MQ=255)

                        gtgcgAAGCCGCACCAGCCGCACCGCCCGGCGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                <  1:3999540/136‑1 (MQ=11)

                           ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGCAGCCGGAGAAGCCGGAGCGACGGCAGcga                                                                                                             >  2:3653110/1‑138 (MQ=11)

                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                            >  2:3564138/1‑140 (MQ=255)

                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                            >  2:700156/1‑140 (MQ=255)

                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGCCGCGACAGGAGCGAc                                                                                                            >  2:1941028/1‑140 (MQ=11)

                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                         >  1:3225379/1‑140 (MQ=255)

                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                         >  2:160636/1‑140 (MQ=255)

                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGACGGAGCGACAGGAGCTACCgg                                                                                                         >  2:1709811/1‑140 (MQ=255)

                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGCACCAACCGGAGCGACAGGAGCTAACCGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                         >  1:5040661/1‑140 (MQ=14)

                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAACAGGACCAACCGGAGCGTCCGGAGCAACAGGCGCATCCGGAAAAGCCGTAGCACCAGGAGCTAcagc                                                                                                         >  2:281745/1‑137 (MQ=11)

                                 cgcaCCATCCGGCGCGACAGGAGCCACCCGGGAAGCCGGACCCACCGGAGCGCCAGGAGCGACCGGCGACGCCGGACCCACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGAGCGACAGGAGCTACCgggg                                                                                                       <  2:1378480/137‑1 (MQ=11)

                                 cGGACCAGCCGGCGCGCCCGGAGCGACCGGGGACGCCGGCCCAGCCGGCGCCACAGGAGCTACCGGGGACGCCGGACCAACCGCAGCGTCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                       <  2:2470167/140‑1 (MQ=14)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCggcg                                                                                                       >  2:4672157/1‑138 (MQ=255)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAACCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                       >  2:3097514/1‑140 (MQ=255)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGCGCCGCCGGACCAGCCGGAGCGACAGGAGCTCCCgggg                                                                                                       >  2:2227764/1‑140 (MQ=14)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCACCCGGACCAACCGCAGCTACCGGAGCGACAGGTGCACCCGGGCCCGCCGGACCGACAGGAGCTACCgggg                                                                                                       >  2:2942108/1‑140 (MQ=14)

                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGAGCAGCCGGAGCGACAGGAGCCACCGgagacg                                                                                                    >  2:1397716/1‑138 (MQ=14)

                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAACCGGAGCTACAGGAGCGACCGGGGAAGCCGGACAGTCCGGAGCGACAGGAGCGACCGGGGCAg                                                                                                    >  2:1248204/1‑140 (MQ=14)

                                          cGGAGCGACCGGCGCTACCGGTGCAGCCGGACCCCCCGGAGCTACCGGTGATACCGGGGAAGCCGGACCCACCGGAGCCACAGGCGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                              <  2:1234378/140‑1 (MQ=14)

                                          cGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                              <  1:3392128/140‑1 (MQ=255)

                                          cGGAGCGACAGGCGCGACCGGGTAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                              <  1:5082916/140‑1 (MQ=14)

                                             aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGCCCAGACGGAGCGACAGGAGCTACCGCGGAAGCCGGACCac                                                                                           >  2:4331260/1‑139 (MQ=14)

                                             aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGTAGCTACCGGGGACGCCGGACCaa                                                                                           >  2:4494710/1‑140 (MQ=255)

                                                  cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGCACCAACCGGa                                                                                      >  2:2441624/1‑140 (MQ=255)

                                                   aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCACCCGGACCCGCCGGAGCGACAGGAGCTCCCGGGGCAGCCGGCCCCCCCGGAg                                                                                     >  2:3028931/1‑140 (MQ=14)

                                                       gCTACCGGGGAATCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGTAGCTACGGGCGACTCCGGACCAGCCGGAGCGACAGGAGCTACCTGGGAAGCCGGACCACACGGAGCGAc                                                                                 >  1:1046431/1‑140 (MQ=11)

                                                        cgcccGGGGAACCCGGCCCCCCCGCAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                <  2:3337061/137‑1 (MQ=14)

                                                        ccaCCGGGCAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                <  2:4882730/138‑1 (MQ=255)

                                                        cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                <  2:3991440/140‑1 (MQ=40)

                                                               ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGccc                                                                         >  1:4254827/1‑139 (MQ=255)

                                                                gccgCCGGCCCCGCCGGCGCGACCGGCGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                        <  1:1849551/137‑1 (MQ=14)

                                                                gacccccgACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGCAGCGACAGGAGCTCCCGGGGAAGCCGTACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                        <  1:4586166/133‑1 (MQ=14)

                                                                 acgCCGGAGCAGCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCATCCTGAGCGTCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACACGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                       <  1:2841902/138‑1 (MQ=14)

                                                                   gCCGGACCCCCCGCAGCCACCGGCGCTCCCGGGGACGCCGGACCACCCGGAGCGACCGGCGCTCCCGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                     <  1:2057267/140‑1 (MQ=14)

                                                                   gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAgg                                                                     <  1:4747267/140‑1 (MQ=255)

                                                                   gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                     <  1:3174148/140‑1 (MQ=255)

                                                                   cccGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                     <  1:1213772/139‑1 (MQ=255)

                                                                    ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                        >  2:757770/1‑136 (MQ=255)

                                                                     cGGACCCCCCGGCGCCACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAGAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                   <  2:4544933/140‑1 (MQ=14)

                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                   <  1:3514727/140‑1 (MQ=255)

                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                   >  1:3715168/1‑140 (MQ=255)

                                                                            gCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                            <  2:34131/140‑1 (MQ=255)

                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                           >  2:521962/1‑140 (MQ=255)

                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGCCGGGGGCCCCCGGACCAGCCGGAGCTACCGGAGCGACCGGGGCGTCCGGCCCACCCGGAGCGACCGGAGCCACCGGGGCGACTggg                                                           >  2:4961451/1‑140 (MQ=14)

                                                                             ccGGAGCCCCAGGAGCTCCCGGTGAACCCGGACCAACCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                           <  1:3908629/140‑1 (MQ=17)

                                                                                 aGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                       <  1:244271/140‑1 (MQ=255)

                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTAGTGGGGACGTCGGa                                                  >  2:1298816/1‑140 (MQ=255)

                                                                                       aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                 <  1:90196/140‑1 (MQ=255)

                                                                                       aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                 <  1:3564138/140‑1 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGCACCCGGCCCACCCGCAGCGACGGGAGCCACGGCGGACACTGTGGACGGCGGAGCAGCAgg                                          >  2:4310964/1‑140 (MQ=14)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                          >  1:2145668/1‑140 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCgg                                          >  2:835330/1‑140 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGAGAAACCGGAACAACCGGAGCGACGGGAGCCACAGGAGCCACTGGCGACCACGGAACAGCAgg                                          >  2:3069994/1‑140 (MQ=14)

                                                                                              aCCGGGGAAGCCGGACAAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGACCCACGTGGTCAACTGGGGACGTCGGACCAGCCgc                                          >  2:3812203/1‑139 (MQ=17)

                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGa                                     >  2:5018060/1‑140 (MQ=255)

                                                                                                   accaGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                   <  1:4489635/107‑1 (MQ=14)

                                                                                                    gAAGCCGGACCCACCGGAGCGACAGGAGCTACGGGGGACGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAACCCGCACCAACCGGACCGCCGGCAGCCACAGGGGCGACAGGGGACGACGGACCAGCAGCAGCGAc                                    >  2:32763/1‑140 (MQ=17)

                                                                                                    gAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                    <  1:1394829/140‑1 (MQ=255)

                                                                                                    gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGAc                                    >  2:4448839/1‑140 (MQ=255)

                                                                                                     caGCCGGACCCACCGGAGCGACAGGCGCGACCGGGGCAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACa                                   <  2:5032300/139‑1 (MQ=21)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                 >  1:702927/1‑140 (MQ=255)

                                                                                                            aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCCACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGACGGAGCTAAAGGAGCTa                            >  1:4176339/1‑140 (MQ=255)

                                                                                                            aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGCGAATCCGGAACATGCGGAGCGACTGGAGCAACAGGGCGTCCTGGGGAGGTAGGTGCTCCCGGCGGGACAGGAGCTa                            >  1:587783/1‑140 (MQ=9)

                                                                                                                aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                        >  1:4522980/1‑140 (MQ=255)

                                                                                                                aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                        >  1:2597967/1‑140 (MQ=255)

                                                                                                                 ccGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                       <  1:1288029/140‑1 (MQ=255)

                                                                                                                     aGCTACAGGCGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                   <  2:4635409/140‑1 (MQ=17)

                                                                                                                     aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                   >  2:2566613/1‑140 (MQ=255)

                                                                                                                      cccaCAGGCGCTACAGGTGACCCCGGACCAGCCGGAGCCCCAGGACCTCCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGCCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                  <  2:3441380/137‑1 (MQ=17)

                                                                                                                          cAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACGGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGACCGGCGCTACCGGGGACGCCGGa              >  2:5150686/1‑140 (MQ=18)

                                                                                                                          cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGa              >  1:868004/1‑140 (MQ=255)

                                                                                                                           aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc             >  1:4844961/1‑140 (MQ=255)

                                                                                                                               gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                        <  1:674856/77‑1 (MQ=39)

                                                                                                                               gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                        >  2:674856/1‑77 (MQ=39)

                                                                                                                               gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAAc         >  2:4730782/1‑140 (MQ=255)

                                                                                                                                cTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                     <  1:1658614/79‑1 (MQ=255)

                                                                                                                                  actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg      >  1:1935915/4‑140 (MQ=255)

                                                                                                                                  actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg      >  1:1333745/4‑140 (MQ=255)

                                                                                                                                  aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCAGGAGCGACGGGAGCCCCAGGGGCGACTGGGGAGGACGGACCAGCCGGAGCGACAGGACCTACCGGTGACGCCGGAACagacgc      >  2:4260085/1‑135 (MQ=17)

                                                                                                                                   ctggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa     >  1:2336038/3‑140 (MQ=255)

                                                                                                                                   ccGGGGAAGCCGGAGCCGCCGGCGCGACAGGAGCGACCGGGGATGCCGGACCATCCGGAGCGCCCGGAGCGACAGGGGCTACTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa     <  2:4844575/140‑1 (MQ=14)

                                                                                                                                   ccGGGGAAGCCGGACCTGCCGGCGCGACAGGAGCTCCCGGCGCAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa     <  2:2306259/140‑1 (MQ=18)

                                                                                                                                   ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa     >  2:4838067/1‑140 (MQ=255)

                                                                                                                                    tggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAg    >  2:2125082/2‑140 (MQ=255)

                                                                                                                                     ggggACGTCGGACCAGCCGGCGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGAGGGGAGCCACAGGGGCTCCTGGGCACGTCGGACCAGCCGGACCGACAGCAGCTCACGGCGACCCCGGACCAACCCGAGc   >  2:1246130/1‑140 (MQ=17)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                        <  1:226380/68‑1 (MQ=40)

GGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCG  >  NZ_CP034943/607562‑607835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`