BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	12.0%	E181D (GAA→GAC) ‡	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	12.0%	403.6 / 26.2	219	E181D (GAA→GAC) ‡	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (99/86); new base C (29/3); total (130/89)
Fisher's exact test for biased strand distribution p-value = 5.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

GGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607562‑607839

ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACGGGGGACGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAg                                                                                                                                            >  1:3040525/1‑140 (MQ=17)

ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                            <  1:2201263/140‑1 (MQ=255)

      gggAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGACCAGGCGGAGCGTCAGGAGCTACCGGCGAAGCCGGAGCAACCGGAGCGACAGGAGCGACCGGGGAATCGGGAc                                                                                                                                      >  1:3098905/1‑140 (MQ=14)

      gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCACGGGGAAGCAGGACCAGCCGGAGCGACAGGAGCGAAAGGGGAAGCCGGAc                                                                                                                                      >  1:4924647/1‑140 (MQ=11)

             tgagcagCTACCGGACAACCCGGACCCCACGGAGCCACAGGAGCAACCGGACAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                               <  2:4752670/134‑1 (MQ=11)

             acaGGGGCTACCGGCGAAGCCGGACCACCCGGAGCTACAGGCGCTACCGGGGAAGCCGGAGCACCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                               <  2:4160027/140‑1 (MQ=11)

                   gCTCCTGGGGAAGCCGGAGCAGCCGGAGCGACTGGAGCTCCCGGTGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                         <  1:3767787/140‑1 (MQ=14)

                   gCTACTGGGCATGCCGGAGGCGCCGGAGCTACAGGAGCTACCGGGGAATCCGGAGCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                         <  2:5091103/140‑1 (MQ=11)

                   gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                         <  2:1333908/140‑1 (MQ=21)

                      ccTGCGGCAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGTGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                      <  1:3279374/139‑1 (MQ=14)

                      aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCCGGAGCGACCGGCGCGGCCTGACCAGCCGGAGCGACCgg                                                                                                                      >  2:3854169/1‑140 (MQ=14)

                      aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                      >  1:605178/1‑140 (MQ=255)

                      aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                      <  2:2650447/140‑1 (MQ=255)

                      aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAAACGGGGCATCAGGACCAACCGGAGCGACAGGAGCAAACGGCGAGCCCGGACCAGCCGGAGCGAGAgg                                                                                                                      >  1:2145846/1‑140 (MQ=14)

                        gtgcgAAGCCGCACCAGCCGCACCGCCCGGCGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                    <  1:3999540/136‑1 (MQ=11)

                           ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGcgc                                                                                                                 >  2:3021299/1‑138 (MQ=14)

                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                                >  2:3564138/1‑140 (MQ=255)

                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                                >  2:700156/1‑140 (MQ=255)

                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                                >  2:1865880/1‑140 (MQ=255)

                             aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCCACCGGAGCTAcc                                                                                                               >  2:244271/1‑140 (MQ=255)

                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                             >  2:160636/1‑140 (MQ=255)

                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGACGGAGCGACAGGAGCTACCgg                                                                                                             >  2:1709811/1‑140 (MQ=255)

                                 cgcaCCATCCGGCGCGACAGGAGCCACCCGGGAAGCCGGACCCACCGGAGCGCCAGGAGCGACCGGCGACGCCGGACCCACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGAGCGACAGGAGCTACCgggg                                                                                                           <  2:1378480/137‑1 (MQ=11)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCggcg                                                                                                           >  2:4672157/1‑138 (MQ=255)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAACCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                           >  2:3097514/1‑140 (MQ=255)

                                 cGGACCACCCGGAGCGCCAGGAGCTTCCGGTGAATCCGGAGCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                           <  2:2768035/140‑1 (MQ=14)

                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGAGCAGCCGGAGCGACAGGAGCCACCGgagacg                                                                                                        >  2:1397716/1‑138 (MQ=14)

                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                        >  1:356801/1‑140 (MQ=255)

                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAACCGGAGCTACAGGAGCGACCGGGGAAGCCGGACAGTCCGGAGCGACAGGAGCGACCGGGGCAg                                                                                                        >  2:1248204/1‑140 (MQ=14)

                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAg                                                                                                        >  2:2525405/1‑140 (MQ=255)

                                          cGGAGCGACCGGCGCTACCGGTGCAGCCGGACCCCCCGGAGCTACCGGTGATACCGGGGAAGCCGGACCCACCGGAGCCACAGGCGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                  <  2:1234378/140‑1 (MQ=14)

                                          cGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                  <  1:3392128/140‑1 (MQ=255)

                                             aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGCCGCGAGCGGAGCACCCGGGGAAGCGGGACCAGCCGGACCCACTGGCGCGACCGGGCAAGCCGGCCCac                                                                                               >  2:5017575/1‑139 (MQ=14)

                                             aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGCCCAGACGGAGCGACAGGAGCTACCGCGGAAGCCGGACCac                                                                                               >  2:4331260/1‑139 (MQ=14)

                                             aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACATGAGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATAGCGGGGAAGCCGGACaaa                                                                                               >  2:4494831/1‑140 (MQ=14)

                                              gccaccggAGCTACCGGGGCACGCGGACGAGCCGGAGCGACAGGAGCAACCTGTGATGCGGGAGGAAACGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGGGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                              <  1:4118662/134‑1 (MQ=14)

                                                 aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGCAGCCGGACCACCCgg                                                                                           >  1:4188194/1‑140 (MQ=255)

                                                       gCTACCGGGGAATCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGTAGCTACGGGCGACTCCGGACCAGCCGGAGCGACAGGAGCTACCTGGGAAGCCGGACCACACGGAGCGAc                                                                                     >  1:1046431/1‑140 (MQ=11)

                                                       gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                     <  2:3225379/140‑1 (MQ=40)

                                                        ccaCCGGGCAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                    <  2:4882730/138‑1 (MQ=255)

                                                        cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                    <  2:357814/140‑1 (MQ=25)

                                                        cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                    <  2:3991440/140‑1 (MQ=40)

                                                               ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGccc                                                                             >  1:4254827/1‑139 (MQ=255)

                                                                gccgCCGGCCCCGCCGGCGCGACCGGCGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:1849551/137‑1 (MQ=14)

                                                                gAAGCCGGACCAGCCGGAGCGACAGGCGCTCCCGGCGAAGCCGGCCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:5003887/140‑1 (MQ=255)

                                                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACCGGAGCACCCGGGGACGCCGGACCAGACGGAGCGCCAGGAGCCACCGGGGAACCCGGACAACCCGGACCGGCGGGAGCcac                                                                            >  2:330166/1‑140 (MQ=14)

                                                                ccacccGGACCACCAGGACCCCCAGGACCAACCGGCCAACCCGGACCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:538295/136‑1 (MQ=14)

                                                                 acgCCGGAGCAGCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCATCCTGAGCGTCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACACGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                           <  1:2841902/138‑1 (MQ=14)

                                                                   gCCGGACCCCCCGCAGCCACCGGCGCTCCCGGGGACGCCGGACCACCCGGAGCGACCGGCGCTCCCGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:2057267/140‑1 (MQ=14)

                                                                   gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAgg                                                                         <  1:4747267/140‑1 (MQ=255)

                                                                   gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:4532932/140‑1 (MQ=255)

                                                                   cccGGACCAGCCGGAGCACCAGGAGGAGCCGGCGAAGCCGGACCCACCGGAGCTCCAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTAAGCAGGACCACCCGGAGCGACGGGAGCCACAgg                                                                         <  1:5051362/139‑1 (MQ=14)

                                                                   cccGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:1213772/139‑1 (MQ=255)

                                                                    ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            >  2:2222952/1‑136 (MQ=255)

                                                                    ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            >  2:757770/1‑136 (MQ=255)

                                                                    ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:757770/136‑1 (MQ=255)

                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  1:3514727/140‑1 (MQ=255)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                    >  2:3664783/1‑140 (MQ=255)

                                                                            gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCAACCGGGGACGCCGGCCCAGCCGGAGCGACAGGCGCGACCGGGGCAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCGACAgg                                                                >  2:3405493/1‑140 (MQ=14)

                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                               >  2:521962/1‑140 (MQ=255)

                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGCCGGGGGCCCCCGGACCAGCCGGAGCTACCGGAGCGACCGGGGCGTCCGGCCCACCCGGAGCGACCGGAGCCACCGGGGCGACTggg                                                               >  2:4961451/1‑140 (MQ=14)

                                                                             ccGGAGCCCCAGGAGCTCCCGGTGAACCCGGACCAACCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                               <  1:3908629/140‑1 (MQ=17)

                                                                                 aGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                           <  1:244271/140‑1 (MQ=255)

                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTAGTGGGGACGTCGGa                                                      >  2:1298816/1‑140 (MQ=255)

                                                                                      cAGGAGCTACCGGCGCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                      <  2:4297231/140‑1 (MQ=255)

                                                                                       aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                     <  1:90196/140‑1 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                              >  1:3184617/1‑140 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCgg                                              >  2:835330/1‑140 (MQ=255)

                                                                                                cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                            >  2:420640/1‑140 (MQ=255)

                                                                                                   accaGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  1:4489635/107‑1 (MQ=14)

                                                                                                   accaGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       >  2:4489635/4‑110 (MQ=14)

                                                                                                    gcaGCCGGACCCACCGCAGCCACACGCGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        <  2:3040525/138‑1 (MQ=21)

                                                                                                    gAAGCCGGACCCCCCGGAGCGACCGGCGCTAGGGGGGTAGCCGGACCCGGCGGAGAGAGAGGAGGTACCGGGGAACGCGGACCAACCGGAGCGAGGGGTGCCACAGTGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        <  2:195598/140‑1 (MQ=18)

                                                                                                    gAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        <  1:1394829/140‑1 (MQ=255)

                                                                                                    gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGAc                                        >  2:4448839/1‑140 (MQ=255)

                                                                                                     caGCCGGACCCACCGGAGCGACAGGCGCGACCGGGGCAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACa                                       <  2:5032300/139‑1 (MQ=21)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                     >  1:702927/1‑140 (MQ=255)

                                                                                                                aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                            >  1:3754825/1‑140 (MQ=255)

                                                                                                                 ccGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                           <  1:1288029/140‑1 (MQ=255)

                                                                                                                     aGCTACAGGCGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                       <  2:4635409/140‑1 (MQ=17)

                                                                                                                      cccaCAGGCGCTACAGGTGACCCCGGACCAGCCGGAGCCCCAGGACCTCCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGCCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                      <  2:3441380/137‑1 (MQ=17)

                                                                                                                          cAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACGGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGACCGGCGCTACCGGGGACGCCGGa                  >  2:5150686/1‑140 (MQ=18)

                                                                                                                               gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:674856/77‑1 (MQ=39)

                                                                                                                               gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            >  2:674856/1‑77 (MQ=39)

                                                                                                                               gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAAc             >  2:4730782/1‑140 (MQ=255)

                                                                                                                                cTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:1658614/79‑1 (MQ=255)

                                                                                                                                  actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:1935915/4‑140 (MQ=255)

                                                                                                                                  actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:1333745/4‑140 (MQ=255)

                                                                                                                                  actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:4547447/4‑140 (MQ=255)

                                                                                                                                  aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  2:2363353/1‑140 (MQ=255)

                                                                                                                                   ctggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa         >  1:2336038/3‑140 (MQ=255)

                                                                                                                                   ccGGGGAAGCCGGAGCCGCCGGCGCGACAGGAGCGACCGGGGATGCCGGACCATCCGGAGCGCCCGGAGCGACAGGGGCTACTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa         <  2:4844575/140‑1 (MQ=14)

                                                                                                                                   ccGGGGAAGCCGGACCTGCCGGCGCGACAGGAGCTCCCGGCGCAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa         <  2:2306259/140‑1 (MQ=18)

                                                                                                                                   ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa         >  2:4838067/1‑140 (MQ=255)

                                                                                                                                    cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGGGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                 <  2:2940112/99‑1 (MQ=255)

                                                                                                                                     ggggACGTCGGACCAGCCGGCGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGAGGGGAGCCACAGGGGCTCCTGGGCACGTCGGACCAGCCGGACCGACAGCAGCTCACGGCGACCCCGGACCAACCCGAGc       >  2:1246130/1‑140 (MQ=17)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:226380/68‑1 (MQ=40)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCgcc    >  1:4627033/1‑138 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGac    <  1:2638344‑M2/140‑3 (MQ=255)

                                                                                                                                          aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgg  >  1:5130951‑M2/1‑136 (MQ=255)

GGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607562‑607839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`