Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,052 T→G 17.0% E573D (GAA→GAC EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0520TG17.0% 407.1 / 25.5 206E573D (GAA→GACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (59/103);  new base G (41/3);  total (100/106)
Fisher's exact test for biased strand distribution p-value = 4.27e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

GTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCG  >  NZ_CP034943/633914‑634182
                                                                                                                                          |                                                                                                                                  
gTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGtt                                                                                                                                   <  2:4673596/140‑1 (MQ=255)
gTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGtt                                                                                                                                   <  2:3597441/140‑1 (MQ=255)
 tCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttc                                                                                                                                  >  2:3891867/1‑140 (MQ=255)
     cATGGATGGCTTGTACCTTTACATGGTCTTGATAGATTTCCCGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttctttc                                                                                                                              <  2:3842907/140‑1 (MQ=255)
     cATGGATGGCTTGTACCTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttgtttct                                                                                                                             >  2:4602806/1‑140 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTc                                                                                                                        >  2:4642348/1‑140 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTc                                                                                                                        <  1:3321701/140‑1 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTc                                                                                                                        <  1:3891867/140‑1 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCtttttg                                                                                                                        >  2:3499799/1‑139 (MQ=255)
             gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTGGTTTGtttttgcg                                                                                                                      >  1:4556240/1‑137 (MQ=255)
             gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTATTTTTGCa                                                                                                                      >  1:2469012/1‑140 (MQ=255)
              cTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAt                                                                                                                     <  2:4808881/140‑1 (MQ=255)
              cTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTGTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAt                                                                                                                     <  1:1011270/140‑1 (MQ=255)
              cTTGTACCATTTCATGGTCTTGATAGATTTCCGGAGCACAGAGCAATTCACCGTTATGACTACTGTATTCTTCAATGGTTTGAACAGTGGCTTCAATGTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAt                                                                                                                     <  1:3591807/140‑1 (MQ=255)
                  tACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCattatt                                                                                                                 >  2:4043707/1‑140 (MQ=255)
                  tACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTACAATCtt                                                                                                                 >  2:4755615/1‑140 (MQ=255)
                       tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTACATTCtttttat                                                                                                            >  1:1226806/1‑138 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTCTTTGTTGTTACATAGTTTttctt                                                                                                           >  2:1305967/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTGCATTCTTTttgtt                                                                                                           >  1:2524651/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTGCATTCTTTttctt                                                                                                           >  1:2550077/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTATTTATTTTTCCATTCTTTttctt                                                                                                           >  2:1562344/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTACATTATTTttatt                                                                                                           >  1:3946283/1‑140 (MQ=255)
                         tCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTCATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGAGTTATTTGTTTTTACATTCTTTttgttc                                                                                                          >  2:4905315/1‑140 (MQ=255)
                         tCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATGCGACGGAGTCGTTGGGGGTCTTTGTTTTTCGATTCTTTttgttg                                                                                                          >  2:4246757/1‑139 (MQ=255)
                         tCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTACATTCTTTttattc                                                                                                          >  2:5128404/1‑140 (MQ=255)
                               tCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTACATTCTTTTTATTATTCata                                                                                                    >  2:1327967/1‑140 (MQ=255)
                               tCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTTCTTTTTCCATTCTTTTTATTCTTCata                                                                                                    >  2:3862942/1‑140 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                  <  2:1122609/140‑1 (MQ=255)
                                  tGATAGATTTCCGTATCACATAGCACTTCATCGTTATGACTAATGTTTTCTTCAATGGTTTTAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGa                                                                                                 <  2:4398053/140‑1 (MQ=255)
                                  tGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGa                                                                                                 <  1:4043707/140‑1 (MQ=255)
                                   gATAGATTTCCGGATCACATAGCTATTCATGGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGaa                                                                                                <  2:196307/140‑1 (MQ=255)
                                       gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                            <  1:845984/140‑1 (MQ=255)
                                         tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGTGCGTTATTTTTCCGGTATTTTTGGTATTCATATTAACGgttt                                                                                          >  2:862412/1‑140 (MQ=255)
                                         tttCCGGATCACAAAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACACTGGCTTCAATTTCTTCAATCCCACTGAGTCGTTGGCGTTATATATTTTTAAATTCTTTTTGTTGTTCAAATTGACCtttt                                                                                          >  2:632800/1‑140 (MQ=255)
                                            ccGGATCACATAGCAATTCCTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAg                                                                                       <  1:1729915/140‑1 (MQ=255)
                                             cGGCTCACATAGCAATTCAGCGTTATGACTAATGTTTTCTTCACTGGATTGAACCGTGGCTTCAATTTCGTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  1:3198331/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  2:246541/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  1:1441964/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  1:5022799/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  1:2389870/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  2:1237277/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCACTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  1:2070202/140‑1 (MQ=255)
                                             agtaTCACAGATCAAGTGATAGTGATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGTCTGAGTCGTTGGCGGTCTGTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                      <  1:4395355/137‑1 (MQ=255)
                                                  cacaTAGCAATTCATCGTTATGACTAATGTTTTCTTGAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATGCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGc                                                                                 <  2:1007976/140‑1 (MQ=255)
                                                  cacaTAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGc                                                                                 <  2:4794640/140‑1 (MQ=255)
                                                       aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                            <  1:3124091/140‑1 (MQ=255)
                                                          aaTTCATCGTTATGACGAATGTTTTCTTCACTGGTTTGAACAGTGGCTTCAATTTCTTCACTCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGa                                                                         <  2:2748968/140‑1 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTGCATTCTTTTTCTTCTTCATATGAAAGTTTTTTTTAAGGGTTTACCGaaa                                                                       >  2:3151677/1‑139 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTCCATTCTTTTTATTCTTCATATTTACGTTTTGAGTGAGGCTTAAACGaac                                                                       >  2:1497695/1‑139 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTGTTTTTCCATTATTTTTGTTCTTCATATTAACGTTTTTAGTCAGGCTTAAaagaag                                                                       >  2:3815959/1‑140 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGGGGTTGTTTTTGCGGTGTTTTTGTTTGTGATATGAACGTTTTTTGTGCGGCTTTACCGAAg                                                                       >  1:2317073/1‑140 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTATTCAATCAGACGGAGTCGTTTGCGTTGTTTGTTTTTGCATTATGTTTCTTCTTGATAAGAAAATTTTTAGTGTGGCATAAACGAAg                                                                       >  1:3707064/1‑140 (MQ=255)
                                                              cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCATTGTTTTTGTTTTTGGTATTAACGTTTTGATTGAGGGTTTAAAGAAGCa                                                                     >  1:1396362/1‑140 (MQ=255)
                                                              cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTATTTTTGGATTCTTTTTGTTGGTCATTTTAAGGGTTTGTGTGATGGGTAACAGAAGCa                                                                     >  1:2876306/1‑140 (MQ=255)
                                                              cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTCCATTCTTTTTATTCTTCATATGAAAGTTTTGAGTAAGGCTTAAACGAAGCa                                                                     >  2:91343/1‑140 (MQ=255)
                                                              cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGGTTTTGCAGTGTTTTTGTTGTTGATATTTACGTTTTGTGTGAGGCTTAACCGAAGcc                                                                     >  1:4460076/1‑139 (MQ=255)
                                                               aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGGTTGTTTTTCCCTTGTTTTTGTTGTTCATTTGAACGTGTTGTGTCAGGCTTAACCGAAGCAg                                                                    >  2:3407771/1‑140 (MQ=255)
                                                               aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAATCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAACGAAGCAg                                                                    >  2:1092560/1‑140 (MQ=255)
                                                               aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGTTTTTGCGTTGTTTTTTTTCTTAAAATGTAGGTTTTTTGTCAGGCTTAAACGAAGCAg                                                                    >  1:4635048/1‑140 (MQ=255)
                                                                 cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCGTTGTTTTTGCCGTCTTTTTGTTATTGATATGAACGTGTTTGGTCCGGCTTTAACGAACCCGGc                                                                  >  2:1752399/1‑140 (MQ=255)
                                                                 cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCGTTATTTTTTCATTCTTTTTCTTCTTCATATTAACGTTTTGTGTCAGGCTTAAACGCAGCCGGc                                                                  >  2:3233344/1‑140 (MQ=255)
                                                                 cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTAAGGCTTAACCGaacccagg                                                                  >  2:5185977/1‑134 (MQ=255)
                                                                    tATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTGCCATCTTTTTATTCTTCATATTAACGTTTTTAGTAATGCGTAAACGAAACAGGCGtt                                                               >  2:2963195/1‑140 (MQ=255)
                                                                        aCTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTCTTTTTCCAATATTTTTATTCTTAATATGAACCTTTTTAGTAAAGCTTAAAACAAGAAGGCGtttttt                                                           >  2:2379682/1‑140 (MQ=255)
                                                                           aaTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCgg                                                        <  2:2840747/140‑1 (MQ=255)
                                                                           aaTGCTTTCTTCAAGGGTATGAACAGTGGCTTCAATGTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTGCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCgg                                                        <  1:4190080/140‑1 (MQ=255)
                                                                            aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCTACGGAGTCGTTGTCTTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCTAAGCAGGCGTTTTTTCGGa                                                       <  2:2699822/140‑1 (MQ=255)
                                                                            aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                       <  2:4537789/140‑1 (MQ=255)
                                                                            aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                       <  2:1381758/140‑1 (MQ=255)
                                                                            aTGTTTTCTTCAATGGTTAGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATCTGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                       <  1:4877110/140‑1 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTATTTTTACATTCTTTTTGTTATTCATATTAAAGTTTTTAGTAAGGCTTAAACGAAGAAGGCGTTTTTTGGGAt                                                      >  2:398846/1‑140 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGAt                                                      <  2:1649876/140‑1 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGAt                                                      <  2:2274591/140‑1 (MQ=255)
                                                                                  tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGGTTGGTTTTCGATTCTTTTTGTTGTTCATATTAAAGTTTTGTTTGAGGCGTTACAGAACGAGTCGGTTTTTgggtgtcatc                                                 >  1:5128623/1‑133 (MQ=255)
                                                                                  tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTCCCTTATTTTTATTATTCATATGAACCTTTTTTGTCAGGCTTAACCGAACCACGCGTTTTTTGGGATTGctc                                                 >  1:4741499/1‑140 (MQ=255)
                                                                                  tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGGTTGTTTTTGCATTATTTTTGTTATTGATATTAAAAGTTTTTTTGATGGTTAAACGAACAAGGGGTTTTTTTGGATTcatg                                                 >  1:2036785/1‑137 (MQ=255)
                                                                                    ttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCCTTCTTTTTGTTCTTCATATTAAAGTTTTGTGTAAGGCTTAAACGAAACAGGCGTTTTTTGGGATTACTGtt                                               >  2:3737514/1‑140 (MQ=255)
                                                                                     tcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTg                                              <  2:2089534/140‑1 (MQ=255)
                                                                                         tGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGa                                          <  1:1343002/140‑1 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTCTTTTTCCCTTGTTTTTCTTCTTGATATGTACGTTTTGAGTGAGGCTTAAACGAATCAGGCGTTTTGTAGGATTCCTCTTGCTGATTCATTTtctgc                              >  2:4997283/1‑138 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGGTTATTTTTGCATTGTTTTTGGTGTTCATTTTGACGTTTTTAGGAAGGCGTAAAAGAAGCAGGGGTTTTTTCTGGTTACGATTGGGGATTCATTTTCTcc                              >  2:1525723/1‑140 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGGTTGTTTTTGCATTGTTTTTTTTAGTCATATGCACGTTTTTATTCAGGGTTAACCGGAGGAGGCGTTTTTTGGGTTTCCTCGTGGTGATTGATTTtgtca                              >  2:4452471/1‑139 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTCCCTTGTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGATTCCTCGTGCTGGTTCAttttgtgc                              >  2:3785313/1‑136 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGGTTGTTTTTGCATTAGTTTTGGTGTTGCTATGTACGTTTTGTGTGAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTGCTCTTGCTGGTTGATTTTGTcc                              >  1:3838912/1‑140 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGTTCTTTGTTTTTTCAGTTTTTTTATTCTTGATATTAACGTTTTGGGTAACGCTTTAACGAACCATCCGTTTTTTAGGATTACTCTTGCTGTTTGATTTTGTcc                              >  1:3721433/1‑140 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGGTGGGGTTGTTTGTTTTTGCCTTCGTTTTGTTCTTCATATGAACGTTTTTGGTGAGGCTTATACGAAGCGGGGGTTTTTTTGGGTTGCTCTGGGTGTTTGGTTTTGTcc                              >  2:152031/1‑140 (MQ=255)
                                                                                                      ggCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTATTTTTGCATTCTTTTTCTTGTTAAAATTAACGTTTTGAGTCAGGCTTAAACCAAGCAGGCGGTTTTTCGGATTCCGCTTTCTGTTTAAGTTTCTCCa                             >  2:2603327/1‑140 (MQ=255)
                                                                                                       gCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTGTTTTTGCATTCTTTTTATTCTTCATAGTCACGGTTTGTGTGACGCTTAAACGAAGCAGGCGTTTTTTGGGATTGCTCTTTGTGATTCATTTTATCCaa                            >  2:1844078/1‑140 (MQ=255)
                                                                                                             aTTTCTTCAATCCGACGGAGTCGTGGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGACCGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAg                      <  1:3958347/140‑1 (MQ=255)
                                                                                                             aTTTATTCAATCCGACGGAGTCTTTGGCTTTCTTTCTTTTTCCATTCTTTTTCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAg                      <  1:2959172/140‑1 (MQ=255)
                                                                                                                 cttcAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTGCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCCGGCGTTTTTTAGGCTTCCTCTTGGTGGTTCAGGTTCTCCCATTCAAgcggc                  >  2:871240/1‑140 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                 <  2:552057/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCATTCTTTTTCTTCTTCCTATGAACGTTTTGAGTCACGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGAGTCATTTTCTCCAATTCAAgctgct                 <  1:1530346/140‑1 (MQ=255)
                                                                                                                  ttcAATCAGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                 <  2:3331447/140‑1 (MQ=255)
                                                                                                                           aCGGATTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTCTGAGTCAGGCTTAACCGAAGCAGGCGTATTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGtttttt        <  1:786757/140‑1 (MQ=255)
                                                                                                                            cGGAGTCGTTGGCGTTCTTTCTTTGTCCATTCTTTTTCTTCTTCATATGAACGTTGTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt       <  1:524607/140‑1 (MQ=255)
                                                                                                                             ggAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtc      <  1:558310/140‑1 (MQ=255)
                                                                                                                              gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct     <  2:4745693/140‑1 (MQ=255)
                                                                                                                                 tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg  <  1:3405865/140‑1 (MQ=255)
                                                                                                                                 tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg  <  1:4905315/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                  
GTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCG  >  NZ_CP034943/633914‑634182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: