BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	165,400	C→T	6.6%	noncoding (2928/2928 nt)	EO946_RS00950 →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	165,400	0	C	T	6.6%	367.6 / 14.6	137	noncoding (2928/2928 nt)	EO946_RS00950	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base C (52/76); new base T (6/3); total (58/79)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 142 total aligned reads displayed.

GCGACACATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGT  >  NZ_CP034943/165354‑165539

gCGACACATGGAGCTGACAGATACTACTCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATa                                                >  1:3850017/1‑140 (MQ=11)

gCGACACATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATa                                                >  1:3634977/1‑140 (MQ=11)

gCGACACATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATa                                                >  1:1621922/1‑140 (MQ=11)

  gACACATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATAcc                                              <  1:1353609/140‑1 (MQ=32)

  gACACATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATAcc                                              <  1:1391095/140‑1 (MQ=32)

  gACACATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATAcc                                              <  1:3856724/140‑1 (MQ=32)

   acacATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCg                                             <  2:4133731/140‑1 (MQ=32)

     acaTGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGaa                                           <  2:3853604/140‑1 (MQ=32)

      caTGGAGCTGACAGATACTCATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAac                                          >  2:749836/1‑140 (MQ=17)

      caTGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAac                                          >  2:1191701/1‑140 (MQ=32)

      caTGGAGCTAACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAac                                          >  2:730220/1‑140 (MQ=17)

        tGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAacac                                        <  2:707493/140‑1 (MQ=32)

        tGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAacac                                        <  2:4608706/140‑1 (MQ=32)

         ggAGCTGACAGATACTAATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAacac                                        <  2:4263230/140‑1 (MQ=12)

          gAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACgg                                      <  2:2025521/140‑1 (MQ=32)

           aGCTGACAGATACTAATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACgg                                      <  1:2632726/140‑1 (MQ=255)

             cTGACAGCTACTAAGCGATCGAGGACTTAACAACA‑TTTTGAATGAAGTCACACCTGTTATCTAGTTTTGAGAGAACAATCTCAATTTTTTTGGTGTCTATAGCGAAGAGGTCACACCCGTTCCCATACCGAACCCGGAAg                                   >  2:2530903/1‑140 (MQ=11)

               gACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGtt                                 >  2:1260850/1‑140 (MQ=32)

                aCAGATACTAATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGtt                                 >  1:4482528/1‑140 (MQ=255)

                aCAGATACTAATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGtt                                 >  1:2169286/1‑140 (MQ=255)

                aCAGATACTAATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACATCTGTGATCGAGTTGTGAGAGTACACTCTGAATTTGTTTGGTGGCGATAGCGAAGAGGTCAGAGCCGTTCCCATACCGAACACGGAAGtt                                 >  1:4643447/1‑140 (MQ=11)

                aCAGATACTAATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGtt                                 >  2:1139017/1‑140 (MQ=12)

                aCAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTa                                <  2:1565985/140‑1 (MQ=32)

                aCAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTa                                >  1:3280026/1‑140 (MQ=32)

                aCAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTAACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGTTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTa                                >  2:5041218/1‑140 (MQ=17)

                 cAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTaa                               >  2:632046/1‑140 (MQ=32)

                 cAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTaa                               >  2:4127134/1‑140 (MQ=32)

                 cAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACACGTTCCCATACCGAACACGGAAGTTaa                               >  2:3327091/1‑140 (MQ=17)

                 cAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACAACCGTTCCCATACCGAACACGGAAGTTaa                               >  2:3056793/1‑140 (MQ=17)

                  ataTACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  2:3097295/138‑1 (MQ=17)

                  ataTACTAATCGATAGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTTGTGGCTATATCGAAGAGGTCACACCCGTTCCCATACCGAAAACGGAAGTTAAg                              <  1:4342428/138‑1 (MQ=11)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCAGACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:4314023/140‑1 (MQ=17)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGCGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:3643526/140‑1 (MQ=17)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  2:1506265/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  2:1149269/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:1287903/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  2:1149034/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  2:3784184/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:4817798/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:1286974/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:2978962/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:3068903/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:1999506/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:4768224/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:4313979/140‑1 (MQ=32)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGGTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  2:1887704/140‑1 (MQ=17)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATATAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:218596/140‑1 (MQ=17)

                  aGATACTAATCGATCGAGGACTTAACCACA‑TTTCGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAg                              <  1:2341549/140‑1 (MQ=17)

                   gATACTAATCGATCGAGGACTTAAACACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTAAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGc                             <  1:5127007/140‑1 (MQ=17)

                     tACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGctc                           >  1:4779990/1‑140 (MQ=32)

                     tACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGctc                           >  2:1298226/1‑140 (MQ=32)

                     tACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGctc                           >  2:4743064/1‑140 (MQ=32)

                     tACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGctc                           >  2:210407/1‑140 (MQ=32)

                         aaTCGATCGAGGACTTAACCACA‑TTTTGAATGATTTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCa                       <  2:1833688/140‑1 (MQ=17)

                         aaTCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCa                       <  2:4957460/140‑1 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:38884/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:3507763/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:1599725/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  1:3710554/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:4392412/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:4440433/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  1:4757200/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:1067755/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  1:938085/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:5191193/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  1:1610474/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  1:1366632/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAg                      >  2:835944/1‑140 (MQ=32)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGATAAGCTCTTCAg                      >  2:2679714/1‑140 (MQ=17)

                          atcgatcgAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACAGAACACGGAAGTTAAGCTCTGCAg                      >  2:4945693/1‑140 (MQ=17)

                            cgatcgaGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAgcg                    <  1:4868442/140‑1 (MQ=32)

                            cgatcgaGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAgcg                    <  1:540885/140‑1 (MQ=32)

                              atcgaGGACTTAACCACA‑TTTTGCATGATGTCAGCCGTGTTATGTAGTGTTGAGACAACCCTCGCACTTTGTTGGGTGGCGTTAGCGAAGAGGTCGCACCCGTTCCCATACCGCACACGGACGTTAAGCTCTTCAGCGcc                  >  1:586232/1‑140 (MQ=11)

                              atcgaGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGcc                  >  2:4387962/1‑140 (MQ=32)

                              atcgaGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGcc                  <  2:1092387/140‑1 (MQ=32)

                              atcgaGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGcc                  >  2:3479021/1‑140 (MQ=32)

                               tcgaGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCg                 >  1:3155404/1‑140 (MQ=32)

                                  aGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAacac                                        >  1:2667145/1‑115 (MQ=11)

                                  aGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAacac                                        <  2:2667145/115‑1 (MQ=11)

                                  aGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATg              >  1:533887/1‑140 (MQ=32)

                                  aGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTGGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATg              <  2:173164/140‑1 (MQ=17)

                                  aGGACTTAACCACA‑TTGTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGGTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATg              <  1:3789355/140‑1 (MQ=17)

                                    gACTTAACCACA‑TTTTGACTGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGt            <  1:2374703/140‑1 (MQ=17)

                                    gACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGt            <  1:2038111/140‑1 (MQ=32)

                                      cTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAg          >  2:1386064/1‑140 (MQ=32)

                                        tAACCACA‑TTTTGAATTATGTGACCCCTGTTATCTAGTTTTGAGAGCACACTCTCATTTTGTTTGGTGGAGATAGCGATGAGGTCACACCGGTTCCCATACCGAACACGGAAGTTAAGCTCTTGATCGCCGATGGTAGTc        <  1:2310170/140‑1 (MQ=11)

                                        tAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTc        <  1:2869340/140‑1 (MQ=32)

                                        tAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTc        <  1:912540/140‑1 (MQ=32)

                                        tAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTc        <  1:5146397/140‑1 (MQ=32)

                                        tAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTc        <  1:2872232/140‑1 (MQ=32)

                                        tAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTCTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTc        <  1:2872210/140‑1 (MQ=17)

                                        tAACCACA‑TTGTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGGGCCGATGGTAGTc        <  2:1871674/140‑1 (MQ=17)

                                         aaCCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCg       <  2:3130463/140‑1 (MQ=32)

                                          cccACA‑TTGACAATGATGGCACACCTGTTATCTAGTTGTCAGAGAACACTCTCAATTTGTATGGTGGCGAGAGCGACGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGGCgg      <  2:4255764/139‑1 (MQ=11)

                                          aCCACA‑TTTTGAATGCTGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTCAGCTCTTCAGCGCCGATGGTAGTCgg      <  2:4255944/140‑1 (MQ=17)

                                          aCCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCgg      <  1:3507763/140‑1 (MQ=32)

                                          aCCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCgg      <  1:1569329/140‑1 (MQ=32)

                                          aCCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCgg      <  2:1755701/140‑1 (MQ=32)

                                          aCCACA‑TTTTGAATGATGGCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCgg      <  2:4646908/140‑1 (MQ=17)

                                              ca‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGt  <  2:4426304/140‑1 (MQ=32)

GCGACACATGGAGCTGACAGATACTAATCGATCGAGGACTTAACCACA‑TTTTGAATGATGTCACACCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGT  >  NZ_CP034943/165354‑165539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`