Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,530 A→C 7.6% Q22P (CAA→CCA) ‡ EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5300AC 7.6% 718.7 / 5.3 288Q22P (CAA→CCA) ‡EO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (178/65);  new base C (2/37);  total (180/108)
Fisher's exact test for biased strand distribution p-value = 1.23e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

TCACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCG  >  NZ_CP034943/755391‑755666
                                                                                                                                           |                                                                                                                                        
 cACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCaa                                                                                                                                         <  1:3222707/140‑1 (MQ=255)
   ccGGTTTTTTCAAGGGAATAGCTTTCCATTATTTATGCCATATTAAACTAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                       <  1:655615/140‑1 (MQ=255)
        tttatCAAGTTAATATCTGTCCATTATTGATGCCATATGAAACTAAAAAATCGAGGGGGAATGAGAAATTGAAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGAAAGCACAACTCAAGGGGGGt                                                                                                                                  <  2:4945999/140‑1 (MQ=255)
                gggAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAg                                                                                                                          >  2:1965492/1‑140 (MQ=255)
                     gagCTTTCCATGATTGATGCCATAAGAAACGAAAAAAACGCGGGGGAATGAGAAATCGCAAAACACATTTATCTTGCTGGCGATTCAACTGTACAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTtct                                                                                                                     <  1:4076567/140‑1 (MQ=255)
                           tacATGATAGATTCAATATGAAACGAAAAAAACGAGGGGTAATGACAAATGGCAAATCAAAGTTATCATGATGGAGATTGAACTCATCAAACGTATCCAGAAAGCACAAATCAAGCAGTGTAGGCGCAGATTCTAGCCTc                                                                                                               >  2:4763311/3‑140 (MQ=255)
                           tCCATTATTTATTCCATATGAAACGAAAAAAACGAGGGGGAATGATAAATGGCAAATCACATTTATCTTGCTTGCGATTCAACTGTTCAAACGTATTGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               <  2:1304877/140‑1 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAATCGAGGGCGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCACGGGGGGTGGGGGGAGTTTCTCGGCTc                                                                                                               >  2:4557871/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTCTCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  2:779980/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  2:2394191/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  1:776662/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  1:2589686/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  1:505777/1‑140 (MQ=255)
                              ctcatagatGCCATATGAAACGCATACAACGAGGGGGATTGAGAAATGGCAAATCACAATTATCTTGCTGGCGATTCACCTGTTCACACGTATGGTGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCa                                                                                                            <  2:3096350/134‑1 (MQ=255)
                               tgatttatTCCATATTAAACTAAAATAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCAt                                                                                                           <  2:3328521/140‑1 (MQ=255)
                               tgatttatTCCATATGAAACTAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCAt                                                                                                           <  2:2199899/140‑1 (MQ=255)
                               tgattgatGCCATATTAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAACTCCAGGGGGGGGGGGGCAGTTTCTCGGCTCGCAt                                                                                                           <  2:615989/140‑1 (MQ=255)
                                gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                          >  1:1067971/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAAGGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:4256335/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCATACGTATGGAGACAGCAAAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGccc                                                                                                     >  2:5183630/1‑139 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:3758685/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGTAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:4424697/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAACTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:4856534/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:2854853/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:1176379/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:2517726/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:4178923/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:1980334/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:3368210/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1976461/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:3630045/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1811409/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1677593/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1360057/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:529746/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATCGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCTCAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:997459/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGCGACAGAGAAATGGCAAATCACATATATCTTGCTGGCGAGTCAACTTTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTCGGGGCAGTTTCTCGGCTCGCATCTGccc                                                                                                     >  2:15720/1‑139 (MQ=255)
                                     aacacATATAAAAACAAAAAAACGAGGGGGAATTATAAATTTCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:2142964/5‑140 (MQ=255)
                                       gcaatatGACACGAAACAAACGAGGGGGAATGAGAAATGGCAAATCCCATTTATCTTGCTGCCGATTCAACTGTTCAAACGTATGCAGCCAGCACACCTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  2:2257313/137‑1 (MQ=255)
                                       gacATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  2:4371488/138‑1 (MQ=255)
                                       gCCATATGATACTATAAAATCTAGGGTGAATGAGAAATGGCAAATCACATTTATCTTTCTGGAGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  2:4646138/140‑1 (MQ=255)
                                       gCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGTAGACAGCACAAATCAAGGGGGGTGGGGGCAGGTTCTCGGCTCGCATCTGCCGGa                                                                                                   >  1:5182185/1‑140 (MQ=255)
                                         tatatTAAAAGTAATAAACGTGGGTGAATGATAAATGGCAAATCACATTTATCTTGCTGGCGATTCAAGTTTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGc                                                                                                 <  2:3052549/139‑1 (MQ=255)
                                           tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGATCat                                                                                               >  1:2718771/1‑140 (MQ=255)
                                           tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                               >  2:2805797/1‑140 (MQ=255)
                                           tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                               >  1:4385827/1‑140 (MQ=255)
                                           tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAAAGTATGGAGACAGCAGAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                               >  2:823441/1‑140 (MQ=255)
                                            atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                       >  2:1119776/1‑99 (MQ=255)
                                              taaaCTAAAAAATAGAGGTGTAATGATAATTGGCAAATCACATTTATCTTGCTGGCGATTCATCTGTTCAAACGTATGGAGAAAGCACAAATCACGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:4537006/139‑1 (MQ=255)
                                               caccGACAAAAACGAGGGGGAATGAGAAATGCCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAACCGTATGGAGACAGCACCAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  1:2365336/137‑1 (MQ=255)
                                               aaaCGAAAAAAACGTGGGGTAAGTATATATGTCACTTCACTTTTATCTTGCTGGCGATTCATCTGTTCAACCGTATGGAGACCGCAACACTCCAGGGGGGTGGGCGCAGGTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:1556954/140‑1 (MQ=255)
                                               aaaCGAAAAAAACGCGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACACCACAAACCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  1:679903/140‑1 (MQ=255)
                                               aaaCGAAAAAAACGAGGTGGAATGAGAAATCTCAAATCACATTTATCTTGCTGGCGATTCAACTGATCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:311392/140‑1 (MQ=255)
                                               aaaCGAAAAAAACGAGGGGGAATGAGAAACGGAAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  1:610363/140‑1 (MQ=255)
                                               aaaCCAACAAAACGAGGTGGAAAGAGCAATGGCACATCACATTGATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGCCAGCACACCACCAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  1:810692/140‑1 (MQ=255)
                                                 acttaaaaaaCGAGGTGTAATTCTTAATGGCATATCACTTTTATCTGGCTGGCGATTCAAGTTTTCAAACGTATGGAGACAGCACAAAACCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:2147376/136‑1 (MQ=255)
                                                 actaaaaataCGAGTGTGAATGAGAAATGGCAAATAACATTTATCTTGCTGGCGATTCTACTGTTCAAACGTATTGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:2013860/137‑1 (MQ=255)
                                                 actaaaaaaaCGTGGGGGAATGATAAATGGCTAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGTGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:1610460/137‑1 (MQ=255)
                                                 accaaacaaacGAGGCGGAATGCGAACTGCCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACCACTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:96339/137‑1 (MQ=255)
                                                 accaaaaaaaCGCGGGGGAACGCGCACTGCCAAATCACCTTTATCTTGCTGGCTCTTCAACTGTTCACACGTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:1708201/137‑1 (MQ=255)
                                                 aCGAAAAAAACGAGGGGGAATGAGAAATGGCATATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:3936347/140‑1 (MQ=255)
                                                 aCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:505777/140‑1 (MQ=255)
                                                           tgAGGTGTAATGATAAATGTCAAATCAATTTTATCTTGCTGGCGATTCTACTGTTCAAACGTATGGAGACATCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGTAGCATATTCAAGTGATTAACa                                                                               <  2:2944625/139‑1 (MQ=255)
                                                              gggggAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTC‑‑‑TGATTAACAGAGCaa                                                                         >  1:1017322/1‑140 (MQ=255)
                                                                         aaaTGGCAAATCACATTTATCTTGATGGCGATTCAACTGTTCAAAAGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCTGCTCGCATCTGCAGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGga                                                                 >  1:4931734/1‑140 (MQ=255)
                                                                                    gaaaTTTATATTTCTGTCGATTCAACTGTTCTAACGTTTGGATAGAGCAAAAATCAAGGGGGGTTGGGGCAGTTTCTCTGCTCGCATCTGCCGGAGCATATTCAAGTTATTAACAGACCAATCTGGTGATGAAGATCGaa                                                      <  2:4207310/137‑1 (MQ=255)
                                                                                      caTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGaaaa                                                    >  1:4691307/1‑140 (MQ=255)
                                                                                       aTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                   >  1:4896977/1‑140 (MQ=255)
                                                                                                 cTGGCGATTCCACTGTTCACCCGTATGCCCCCCGCACACCTCCCGGCGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                         <  2:4178923/140‑1 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAGATTTGTGGaa                                         >  2:1366133/1‑140 (MQ=255)
                                                                                                   ggCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAaga                                       >  2:3999468/1‑140 (MQ=255)
                                                                                                        ttCATCTGTTCAACAGTCTCGCGCCCGCACAAATCCAGGGGGGCTCGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCCTATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCa                                  <  2:1741160/140‑1 (MQ=255)
                                                                                                              tGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTc                            >  2:4980130/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACCGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                          >  2:717318/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                          >  2:450997/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                          >  2:3588012/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                          >  2:250344/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                          >  2:1392905/1‑140 (MQ=255)
                                                                                                                  cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAATTGATTAACAGATCAATCGGGGGACGAAGCTCGAAAACATTTGTGGAAGAGGGCAGTCTTCAgtc                        >  1:4664649/1‑138 (MQ=255)
                                                                                                                  cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                        >  2:4109951/1‑140 (MQ=255)
                                                                                                                        tctGGAGACAGCACAACTCACGGGGGGTCGGGGCAGTTGCTCCGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGCGGAAGAAGCTCGAAAACATGTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                  <  2:253989/138‑1 (MQ=255)
                                                                                                                        aaTGGAGACACCACAAATCATGGGGGCTGGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                  <  2:3210979/139‑1 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                >  1:2794637/1‑140 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                >  1:3918675/1‑140 (MQ=255)
                                                                                                                            gagagaGCACTATTCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAt              <  2:2865969/140‑1 (MQ=255)
                                                                                                                               aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg           >  1:962872/1‑140 (MQ=255)
                                                                                                                               aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg           >  2:2695133/1‑140 (MQ=255)
                                                                                                                               aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg           >  1:3861844/1‑140 (MQ=255)
                                                                                                                                catatcaAAACAAGCGGGGCGGTCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGTGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGa          <  2:1913311/135‑1 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt         >  2:445078/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt         >  1:3130261/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt         >  2:370690/1‑140 (MQ=255)
                                                                                                                                 aGCAAAAATCCCGGGTGGTGGTGTCAGTTTCTCGGCTCGCATATGCCGGAGCATATTCAAGGGATTAACAGATCAACCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt         <  2:54492/140‑1 (MQ=255)
                                                                                                                                  gCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAtt        >  1:1721824/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:3959444/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:3590828/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:3453764/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:5123525/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:1427365/1‑140 (MQ=255)
                                                                                                                                    acaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGa      >  1:4110131/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TCACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCG  >  NZ_CP034943/755391‑755666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: