Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,531 A→C 8.2% Q22H (CAA→CAC) ‡ EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5310AC 8.2% 673.8 / 6.1 288Q22H (CAA→CAC) ‡EO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (178/70);  new base C (2/34);  total (180/108)
Fisher's exact test for biased strand distribution p-value = 8.68e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCG  >  NZ_CP034943/755392‑755666
                                                                                                                                           |                                                                                                                                       
cACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCaa                                                                                                                                         <  1:3222707/140‑1 (MQ=255)
  tatgTTTTTTCAATTGAAGTTCTTTCATTGATTTATGTAATATGAAACTAAAAAAACGAGTGTGAATTAGAAATTGCAAATCACATTTATGTTACTGGCGATTCAACTTTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                       <  2:2986798/137‑1 (MQ=255)
                    gagCTTTCCATGATTGATGCCATAAGAAACGAAAAAAACGCGGGGGAATGAGAAATCGCAAAACACATTTATCTTGCTGGCGATTCAACTGTACAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTtct                                                                                                                     <  1:4076567/140‑1 (MQ=255)
                          tCCTTGATTGATGCCATATGAACCGAAAAAATCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               <  2:2069503/140‑1 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  2:3313399/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  1:505777/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  2:2394191/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  1:776662/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                               >  1:2589686/1‑140 (MQ=255)
                             aTGATTGATTCCATATTAAACGAAAAAATCTAGGGTGAATTAGAAATGTCAAATCACATTTATCTTGCTGGCGATTCAACTGTTAAAACGTATGTAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCa                                                                                                            <  2:5050204/140‑1 (MQ=255)
                              tgattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTAGGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCAt                                                                                                           <  2:760562/140‑1 (MQ=255)
                               gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                          >  1:1067971/1‑140 (MQ=255)
                                 ttgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTg                                                                                                        >  1:2433993/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAAGGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:4256335/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:2854853/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:4848270/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:3368210/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:523477/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1976461/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:444498/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1811409/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:4147885/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  2:669479/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1677593/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:1360057/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:4178923/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATCGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCTCAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:997459/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGCGACAGAGAAATGGCAAATCACATATATCTTGCTGGCGAGTCAACTTTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTCGGGGCAGTTTCTCGGCTCGCATCTGccc                                                                                                     >  2:15720/1‑139 (MQ=255)
                                    aacacATATAAAAACAAAAAAACGAGGGGGAATTATAAATTTCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                     >  1:2142964/5‑140 (MQ=255)
                                      tccATATGAAACTAAAAAAACGAGGGGGAATGATAAATGGCAAATGAGATTTTTCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  2:339823/139‑1 (MQ=255)
                                      gctatatGAAACTCAAAAAACGATTTGGAATGAGAAATGGAATATCTCATTTATCTTGCTGGAGATTCACCTTTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  2:1169793/137‑1 (MQ=255)
                                      gcaatatGACACGAAACAAACGAGGGGGAATGAGAAATGGCAAATCCCATTTATCTTGCTGCCGATTCAACTGTTCAAACGTATGCAGCCAGCACACCTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  2:2257313/137‑1 (MQ=255)
                                      gCCATATGAAACGAAAAAAACGCGGGGGAATGAGAAATGGCACAGCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGACACAGCACACCTCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  1:4306872/140‑1 (MQ=255)
                                      gCCATATGAAACGAAAAAAACGAGGGGGAATGAGAACTCCCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGACACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                   <  1:3500992/140‑1 (MQ=255)
                                      gCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGTAGACAGCACAAATCAAGGGGGGTGGGGGCAGGTTCTCGGCTCGCATCTGCCGGa                                                                                                   >  1:5182185/1‑140 (MQ=255)
                                        cATATGAAACTAAAAAATCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCTATTCAACTGTTCAACCGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGc                                                                                                 <  2:4349645/140‑1 (MQ=255)
                                        cATATGAAACGAAAAACACGAGTGTGAATGAGAAATGGCAAATCACATTTATCTGGCTGGCTATTCAACTGTTCAAACGTATGGAGACATCACAAATCAAGGGGGGTGGGGGCAGTGTCTCGGCTCGCATCTGCCGGAGc                                                                                                 <  2:1343714/140‑1 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGATCat                                                                                               >  1:2718771/1‑140 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                               >  1:598313/1‑140 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                               >  2:4068285/1‑140 (MQ=255)
                                           atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                       >  2:1119776/1‑99 (MQ=255)
                                             taaaCGAAAAAAACGAGTGGTAATGAGAAATGTCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:4536942/139‑1 (MQ=255)
                                              caccGACAAAAACGAGGGGGAATGAGAAATGCCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAACCGTATGGAGACAGCACCAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  1:2365336/137‑1 (MQ=255)
                                              aaaCTAAAAATAAGATGGGTAATGAGATATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:2687048/140‑1 (MQ=255)
                                              aaaCTAAAAAAACGAGGGTGAATGAGACATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:1780102/140‑1 (MQ=255)
                                              aaaCGAAATAAACGTTGTGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTTTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:2765836/140‑1 (MQ=255)
                                              aaaCGAAATAAACGAGCGTCAATGAGAAATGCAAAATCACATTTATCTTGCTGGCGATTCACCTTTTCAAACGTATGGACACAGCACTTATGTGGGGGGGTGGGGCCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:1949214/140‑1 (MQ=255)
                                              aaaCGAAAAAAACGAGGTGGAATGAGAAATCTCAAATCACATTTATCTTGCTGGCGATTCAACTGATCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  2:311392/140‑1 (MQ=255)
                                              aaaCCAACAAAACGAGGTGGAAAGAGCAATGGCACATCACATTGATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGCCAGCACACCACCAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           <  1:810692/140‑1 (MQ=255)
                                                actaaaaaaaCGTGGGGGAATGATAAATGGCTAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGTGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:1610460/137‑1 (MQ=255)
                                                accaaacaaacGAGGCGGAATGCGAACTGCCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACCACTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:96339/137‑1 (MQ=255)
                                                accaaaaaaaCGCGGGGGAACGCGCACTGCCAAATCACCTTTATCTTGCTGGCTCTTCAACTGTTCACACGTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                         <  2:1708201/137‑1 (MQ=255)
                                                 cGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAg                                                                                        >  2:370571/1‑140 (MQ=255)
                                                  gAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGt                                                                                       >  1:649595/1‑140 (MQ=255)
                                                             gggggAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTC‑‑‑TGATTAACAGAGCaa                                                                         >  1:1017322/1‑140 (MQ=255)
                                                                        aaaTGGCAAATCACATTTATCTTGATGGCGATTCAACTGTTCAAAAGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCTGCTCGCATCTGCAGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGga                                                                 >  1:4931734/1‑140 (MQ=255)
                                                                           tGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaaga                                                              >  1:3696368/1‑140 (MQ=255)
                                                                            ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                             >  2:4958025/1‑140 (MQ=255)
                                                                            ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                             >  2:4624942/1‑140 (MQ=255)
                                                                            ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                             >  2:4492836/1‑140 (MQ=255)
                                                                                   gtcttttATGTTGATGGCGATTCATCTGTTCAATGGTCTGGAGACAGCATTTTTCACGGGGTGTGGGGGCAGTTTCTCTGCTCGCATCTGCCGGAGCATATTCTATTGATTAACAGATCAATCGGGGGTAGAAGCTCgta                                                      <  2:2926689/136‑3 (MQ=255)
                                                                                   gaaaTTTATATTTCTGTCGATTCAACTGTTCTAACGTTTGGATAGAGCAAAAATCAAGGGGGGTTGGGGCAGTTTCTCTGCTCGCATCTGCCGGAGCATATTCAAGTTATTAACAGACCAATCTGGTGATGAAGATCGaa                                                      <  2:4207310/137‑1 (MQ=255)
                                                                                   cactgctATCTTGCCGCCGATTCACCTGTTCCCCCGTATGCAGCCAGCACAAAGCAAGGGGCCTGGGGCCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGaa                                                      <  2:811599/134‑1 (MQ=255)
                                                                                     caTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGaaaa                                                    >  1:4691307/1‑140 (MQ=255)
                                                                                      aTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                   >  1:4896977/1‑140 (MQ=255)
                                                                                        ttATCTTGCTTGACATTCACCTGTCCAAACGTCCGCAGACACCACAACTCCCGGGGGGTGGGGGCAGTGTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAt                                                 <  1:679595/140‑1 (MQ=255)
                                                                                        ttATCTTGCTGGCTATTCACCTGTTCAAACGTATGGGGACAGCACCACTCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAt                                                 <  2:542742/140‑1 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                         >  2:219859/1‑140 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                         >  2:1487387/1‑140 (MQ=255)
                                                                                                     taTTCAAGTGTTCCACAGTAGTGAGCGAGCACAACTCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAggg                                    <  2:3994867/139‑1 (MQ=255)
                                                                                                       ttCATCTGTTCAACAGTCTCGCGCCCGCACAAATCCAGGGGGGCTCGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCCTATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCa                                  <  2:1741160/140‑1 (MQ=255)
                                                                                                            gaggacAAGCGTCTGGAGACTGCACACCGCCCGTGGGGGCGGGGCAGTTTCTCGGCGCGCATCTGCCGGAGCATAGTCAACTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACACTTGTGGAAGACGGCAGGCtt                             <  1:4404290/135‑1 (MQ=255)
                                                                                                             tGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTc                            >  2:3090493/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                          >  2:4921969/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                          >  2:250344/1‑140 (MQ=255)
                                                                                                                 ccaaCGTCTGGAGCAAGCACACATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCTTATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                        <  2:4539632/138‑1 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                        >  2:597252/1‑140 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                        >  2:3778310/1‑140 (MQ=255)
                                                                                                                       tATCGAGACAGCACATCTCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                  <  2:253308/140‑1 (MQ=255)
                                                                                                                       aaTGGAGACACCACAAATCATGGGGGCTGGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                  <  2:3210979/139‑1 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                >  1:4628817/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                >  2:4730770/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                >  1:3918675/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                >  2:120798/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                >  1:2794637/1‑140 (MQ=255)
                                                                                                                              aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg           >  1:3861844/1‑140 (MQ=255)
                                                                                                                              aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg           >  1:962872/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGTGCAGGCTTCAGGCGATTCTTTATGTGAt         >  2:5147290/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt         >  2:198848/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt         >  1:3130261/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCGCGGCTCTCATCTGCCGGAGCAGATTCAAGTGATTAACAGAGCAATCGGGGGAAGACGCTCGACAACATTTGTGTAAGAGGGCAGCCTTCAGGCGATTCTTGATGTGAt         >  2:2843747/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTATCGGATCGCATCTGCCGGAGCATATTAAAGTGATTAACATATCAATCGGGGGAAGAAGCTCGAAAACATTTGTGCAAGAGGGAATGCTTCAGGCGATTCTTGATGTGAt         >  2:3627149/1‑140 (MQ=255)
                                                                                                                                 gCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAtt        >  1:1721824/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                           >  2:1379528/1‑56 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:3453764/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:3959444/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:5123525/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg       >  1:1427365/1‑140 (MQ=255)
                                                                                                                                   acaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGa      >  1:4110131/1‑140 (MQ=255)
                                                                                                                                       aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg  >  2:1975266/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCG  >  NZ_CP034943/755392‑755666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: