BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	814,356	T→G	13.5%	H340Q (CAT→CAG)	nosA →	nitric oxide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	814,356	0	T	G	13.5%	923.2 / 80.3	380	H340Q (CAT→CAG)	nosA	nitric oxide synthase
Reads supporting (aligned to +/- strand): ref base T (123/198); new base G (56/3); total (179/201)
Fisher's exact test for biased strand distribution p-value = 1.82e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

AGCAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCTTA  >  NZ_CP034943/814217‑814494

aGCAAGGTGTCAGCATCGTGGACCCTCATACGGCCGCCCGCCAGTTTACACGGTTTGAAGAACAGGAGGACGAACCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCCCTCat                                                                                                                                            <  2:1736968/140‑1 (MQ=255)

  cAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCagat                                                                                                                                          >  1:4134371/1‑140 (MQ=255)

      gtgtCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTc                                                                                                                                      <  1:242536/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGACGAACAGGAGGAAGAAGCGGGCAGAAGGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  2:1846436/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  2:2676998/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  >  2:1518848/1‑140 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  1:5023065/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCAcc                                                                                                                                  >  2:5183941/1‑140 (MQ=255)

          cAGCATCGTGGACCATCAGACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  1:1224487/140‑1 (MQ=255)

                cGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCt                                                                                                                            >  2:3879033/1‑140 (MQ=255)

                cGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCt                                                                                                                            >  1:104808/1‑140 (MQ=255)

                 gtctacaatcatACGGCCGAAATCAAATTTAAACGGTTTGAATAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTa                                                                                                                           >  2:4697859/8‑140 (MQ=255)

                  tGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTAt                                                                                                                          >  1:158990/1‑140 (MQ=255)

                  tGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTAt                                                                                                                          >  1:1856458/1‑140 (MQ=255)

                    gACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCAACCGCTGCCACTCATATCTTCCACCGTTCCTATGa                                                                                                                        <  2:2571930/140‑1 (MQ=255)

                    gACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGACTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGa                                                                                                                        <  2:3779858/140‑1 (MQ=255)

                      ccATCATACGGCCGCAAGCCCGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTAATTCCGCCGATTTCACCCGCTGCCACTCAGAGCTTCCACCGTTCCTATGACa                                                                                                                      >  1:4959993/1‑140 (MQ=255)

                        atcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAAc                                                                                                                    >  1:2892983/1‑140 (MQ=255)

                        atcatACGGCCGCAAGCCAGTTTAAACGCTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACGCAGAGCTTCCACCGTTCCTAGGACAAc                                                                                                                    >  1:669369/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACt                                                                                                                   >  1:87034/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAAcg                                                                                                                   >  2:2047888/1‑139 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGAGTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACt                                                                                                                   >  2:3279211/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGTCGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATCTCTTCCACCGTTCCGATGACAACTCAATCGtt                                                                                                           >  2:2768410/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                           >  2:2469917/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                           >  1:302995/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                           >  1:5052985/1‑140 (MQ=255)

                                   gCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTaa                                                                                                         >  2:141943/1‑140 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                        >  2:4579744/1‑140 (MQ=255)

                                     aaGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTcc                                                                                                                             <  2:5097356/118‑1 (MQ=255)

                                     aaGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTcc                                                                                                                             <  2:2375038/118‑1 (MQ=255)

                                         cAGTTTAAACGGTTTGAAGAACAGGAGGAAGTAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGaa                                                                                                   <  2:891229/140‑1 (MQ=255)

                                         cAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGaa                                                                                                   <  2:3640484/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:2968319/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:4761685/140‑1 (MQ=255)

                                                      ttGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAg                                                                                      >  1:710730/1‑140 (MQ=255)

                                                      ttGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTACCAg                                                                                      >  2:1590505/1‑140 (MQ=255)

                                                        gaagaaCAGGAGGAAGAAGCGGGCAGAAATCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGa                                                                                    <  2:5050928/140‑1 (MQ=255)

                                                        gaagaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGa                                                                                    <  2:2909991/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:3589476/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:1725674/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:949857/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:2527577/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:4553137/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:4367383/140‑1 (MQ=255)

                                                               aggaggAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                             <  2:3709947/140‑1 (MQ=255)

                                                               aggaggAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                             <  1:1994888/140‑1 (MQ=255)

                                                                 gaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTa                                                                           <  1:4342241/140‑1 (MQ=255)

                                                                 gaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTa                                                                           <  2:3580141/140‑1 (MQ=255)

                                                                 gaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTa                                                                           <  2:4524329/140‑1 (MQ=255)

                                                                   ggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACg                                                                         >  2:3206935/1‑140 (MQ=255)

                                                                   ggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAACAGGATAAGCCTTACg                                                                         >  2:868894/1‑140 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCCCTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaacaac                                                               >  2:926031/1‑140 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTCATCAGGATa                                                                                  <  2:3453547/121‑1 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTCATCAGGATa                                                                                  >  1:3453547/1‑121 (MQ=255)

                                                                                  aaaGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATTAGGATAAGCCTTACGAGTAAACAACGGaaa                                                          <  2:700606/140‑1 (MQ=255)

                                                                                    aGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGAGTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGGTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGaaaag                                                        <  1:1319247/140‑2 (MQ=255)

                                                                                          cGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGa                                                  <  2:3368377/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  1:2832509/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  1:30955/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  2:4414206/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  1:1961164/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  2:2593401/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGAGAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  2:4962888/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTGAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  1:215066/140‑1 (MQ=255)

                                                                                            ggggACTGGACGGGGCGGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  2:4269752/140‑1 (MQ=255)

                                                                                                   ggACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGCTAAGCCTTACGAGTAAACAACGGAAAATCGGTGTTGGCCTTCa                                         >  2:4805083/1‑140 (MQ=255)

                                                                                                   ggACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                >  1:1536223/1‑133 (MQ=255)

                                                                                                   ggACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCa                                         >  2:3547544/1‑140 (MQ=255)

                                                                                                   ggACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCa                                         >  1:4000711/1‑140 (MQ=255)

                                                                                                    gACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCaa                                        <  2:3975129/140‑1 (MQ=255)

                                                                                                    gACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCaa                                        <  2:1289194/140‑1 (MQ=255)

                                                                                                    gACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCaa                                        <  2:1549974/140‑1 (MQ=255)

                                                                                                       gTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGc                                     >  2:114327/1‑140 (MQ=255)

                                                                                                        tGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCt                                    >  1:1497911/1‑140 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                   <  2:2856981/140‑1 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                   <  2:2728958/140‑1 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                   <  2:2315476/140‑1 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                   >  2:4141400/1‑140 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                   >  1:2089671/1‑140 (MQ=255)

                                                                                                         ggCTGATTCCGCCGAGTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                   <  2:1784042/140‑1 (MQ=255)

                                                                                                            tGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCCTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCgg                                <  1:5145637/140‑1 (MQ=255)

                                                                                                              aTTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGa                                                                                    <  2:2110921/86‑1 (MQ=255)

                                                                                                              aTTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGa                              <  2:3072853/140‑1 (MQ=255)

                                                                                                              aTTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGa                              <  1:3207082/140‑1 (MQ=255)

                                                                                                              aTTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGa                              <  2:1944392/140‑1 (MQ=255)

                                                                                                               ttCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGAt                             >  2:1892928/1‑140 (MQ=255)

                                                                                                                 ccgccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTGCGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTc                           <  2:558242/140‑1 (MQ=255)

                                                                                                                            aCCCTCTGCCACTCATATCTTCCACCGTTCCTATGACCACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga                <  2:4252385/140‑1 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga                <  2:4225345/140‑1 (MQ=255)

                                                                                                                                   tccACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  1:4463630/139‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACACCGGAAACTCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  2:2171663/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  1:2187325/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  1:2766278/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  2:760863/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  1:3749118/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  1:3832459/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  2:2255193/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  2:4088222/140‑1 (MQ=255)

                                                                                                                                       cTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAAcc     <  2:2212092/140‑1 (MQ=255)

                                                                                                                                        tCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCt    <  2:1018541/140‑1 (MQ=255)

AGCAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCTTA  >  NZ_CP034943/814217‑814494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`