Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 847,234 C→A 6.3% C284F (TGT→TTT)  EO946_RS04305 ← NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943847,2340CA 6.3% 787.1 / 11.9 298C284F (TGT→TTT) EO946_RS04305NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (101/167);  new base A (24/3);  total (128/170)
Fisher's exact test for biased strand distribution p-value = 3.46e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TGCAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCA  >  NZ_CP034943/847096‑847372
                                                                                                                                          |                                                                                                                                          
tgCAATCCCAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACa                                                                                                                                           >  2:4071026/1‑140 (MQ=255)
tgCAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACa                                                                                                                                           >  2:2363594/1‑140 (MQ=255)
tgCAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACa                                                                                                                                           <  2:2338084/140‑1 (MQ=255)
tgCAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACa                                                                                                                                           >  2:4909229/1‑140 (MQ=255)
tgCAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACa                                                                                                                                           >  2:3526743/1‑140 (MQ=255)
tgCAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCGTTGTAACGAGAAGAGCCTCTCCGTAAACGGAGAGGCTCTCGAATTATTTTATACa                                                                                                                                           >  2:3404317/1‑140 (MQ=255)
  cAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACAtt                                                                                                                                         >  1:3595973/1‑140 (MQ=255)
  cAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCACCTTAAACGGAGAGGCTCTCTAATTATTTTATACAtt                                                                                                                                         >  1:3709907/1‑140 (MQ=255)
  cAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCGCACCTTAAACGGAGAGGCTCTCTTAATATTTTATACagt                                                                                                                                         >  1:1014815/1‑138 (MQ=255)
     tCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTTAACGGAGAGGCTCTCGAATTATTTTATATAttttt                                                                                                                                      >  2:384955/1‑139 (MQ=255)
     tCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATAAATTTta                                                                                                                                      >  1:1350182/1‑140 (MQ=255)
     tCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCATAAAAGGAGAGGGTGTCTAATTATTTTATAAAATTta                                                                                                                                      >  1:1507235/1‑140 (MQ=255)
      cacaGATCTGAAAAAACAAGGCTACCCATTTAAAAGCCTTGATGATTTAATGTTGGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCGCTCCTTAAACGGAGAGGCTCTCTAAGTATTTTATACATTTtat                                                                                                                                     <  2:2942755/140‑1 (MQ=255)
      cacaGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTtat                                                                                                                                     >  2:3910941/1‑140 (MQ=255)
      cacaGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCGCGCGAATTATTTTATACATTTtat                                                                                                                                     >  2:2445055/1‑140 (MQ=255)
      cacaGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAAAGGAGAGGCTCGCTAATTATTTTATAAAATTtat                                                                                                                                     >  2:4534933/1‑140 (MQ=255)
      cacaGATCAGAAAAAACAAGGCTACACATCTAAAACCCTTGATGATAGAAGGCTTGAGAACGAAATCACGCTGCCGTCTTTGTAACGAGACGAGCCTCTCCTTAAACGGAGAGGCTCTCTACTTATGTTATACATTTtat                                                                                                                                     <  2:940504/140‑1 (MQ=255)
           aTCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGGCTCTGCTTAAAAGGAGAGGGGCTGGAATTATTTTTTAAATTTTATaattt                                                                                                                                >  1:398299/1‑137 (MQ=255)
           aTCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTg                                                                                                                                <  2:4750877/140‑1 (MQ=255)
           aTCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTg                                                                                                                                >  2:5004242/1‑140 (MQ=255)
           aTCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTg                                                                                                                                <  1:5105142/140‑1 (MQ=255)
                acaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAAGGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGcc                                                                                                                           <  2:3089364/138‑1 (MQ=255)
                aaaaaaCAAGGCTACGCATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACCGAGAGGCACTCTAAATATTTTATACAATTTATAAGTGCGGcc                                                                                                                           >  2:4668876/1‑140 (MQ=255)
                aaaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGcc                                                                                                                           <  1:2507657/140‑1 (MQ=255)
                aaaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGcc                                                                                                                           <  1:1987492/140‑1 (MQ=255)
                aaaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGcc                                                                                                                           <  2:2014238/140‑1 (MQ=255)
                aaaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGcc                                                                                                                           >  2:2552829/1‑140 (MQ=255)
                aaaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGcc                                                                                                                           <  2:3032272/140‑1 (MQ=255)
                aaaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAAAGGAGAGGCTCTCTAATTATTTTATAAATTTTATAAGTGCGGcc                                                                                                                           >  2:1415649/1‑140 (MQ=255)
                aaaaaaCAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAAGGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGcc                                                                                                                           <  2:2173818/140‑1 (MQ=255)
                              cacaTTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGGCGCGCCGTAAAAGGAGAGGCGGGGTAATTATTTTATAAATTTTTTAATTGAGGCCCCATCCAAGTCTcc                                                                                                             >  1:1809581/1‑140 (MQ=255)
                              cacaTGTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAAAGGAGCGGCTCGCGAATTATTTTATACATGTTATAAGTGCGGCCCCATCCaatggggc                                                                                                             >  1:3836241/1‑134 (MQ=255)
                                   ttAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGGGGCCCAATACACGTGTCCGAAGc                                                                                                        >  2:3468065/1‑140 (MQ=255)
                                   ttAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCCATCCAACTCTCCGAAGc                                                                                                        >  2:588102/1‑140 (MQ=255)
                                        aGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCACTCCTTAAACGGCGAGGCTCTCGAATTATTTTATACAATTTATAAGTTCGGCCCAAAACAAATATACGAAGCCtttt                                                                                                   >  2:771698/1‑140 (MQ=255)
                                                           ttGAGAAAGCAATGAAGCTGCCGTCTTGGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAAt                                                                                <  2:1780196/140‑1 (MQ=255)
                                                            tGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGGTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAAtt                                                                               >  2:229945/1‑139 (MQ=255)
                                                               gaaagaaaTGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGcc                                                                            <  2:1235002/140‑1 (MQ=255)
                                                                     aaTGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGc                                                                      <  2:2767093/140‑1 (MQ=255)
                                                                        gAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAg                                                                   <  1:405892/140‑1 (MQ=255)
                                                                          aGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCt                                                                 <  1:640709/140‑1 (MQ=255)
                                                                          aGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCt                                                                 <  1:670766/140‑1 (MQ=255)
                                                                               ccGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCa                                                            <  1:4534933/140‑1 (MQ=255)
                                                                               ccGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCa                                                            <  1:3910941/140‑1 (MQ=255)
                                                                                         aaCGAGAAGCGCCTCGCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCACTCCCAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCGCTTGGCCAGCTCGGCACGaggtaggt                                                  <  2:3231644/140‑1 (MQ=255)
                                                                                         aaCGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtaggt                                                  <  2:1619631/140‑1 (MQ=255)
                                                                                         aaCGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtaggt                                                  <  2:1580546/140‑1 (MQ=255)
                                                                                         aaCGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtaggt                                                  <  1:4017029/140‑1 (MQ=255)
                                                                                         aaCGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtaggt                                                  <  1:463602/140‑1 (MQ=255)
                                                                                         aaCGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtaggt                                                  <  1:4909229/140‑1 (MQ=255)
                                                                                              gaagaGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCa                                             <  2:1483285/140‑1 (MQ=255)
                                                                                              gaagaGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCa                                             <  2:3593596/140‑1 (MQ=255)
                                                                                              gaagaGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCa                                             <  1:4668303/140‑1 (MQ=255)
                                                                                                   gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                        <  1:4359284/140‑1 (MQ=255)
                                                                                                   gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                        <  2:398299/140‑1 (MQ=255)
                                                                                                   gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                        <  1:2436222/140‑1 (MQ=255)
                                                                                                   gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                        <  2:1938859/140‑1 (MQ=255)
                                                                                                       ctcCTTAAACGGAGAGGCTGTCTAATTATTTTATAAATTTTATAATTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAAAGGCCCATGGCCAGCGCGGCACGAGGTAGGTTTGCAGAGGCCTGt                                    >  2:4378445/1‑140 (MQ=255)
                                                                                                       ctcCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTACGCTTTCAAAATGGCCCATGGCCATCTCGGCACGAGGTCGGTTTGCATAGGCCTGt                                    >  2:3710081/1‑140 (MQ=255)
                                                                                                         ccTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTGAAGTCCGCTTTCAAAATGGCCCTTGGGCAGCTCGGCAAGAGGTACGTTTGCAGAGGCCTggtg                                  >  2:3923669/1‑137 (MQ=255)
                                                                                                                  gagaGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggc                         >  1:532026/1‑140 (MQ=255)
                                                                                                                  gagaGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggc                         <  2:4950212/140‑1 (MQ=255)
                                                                                                                  gagaGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggc                         >  1:325894/1‑140 (MQ=255)
                                                                                                                  gagaGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGGAGGTTTGCAGAGGCCTGTTCTgcggcggc                         >  2:5161763/1‑140 (MQ=255)
                                                                                                                   agagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                        <  1:4966568/140‑1 (MQ=255)
                                                                                                                   agagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                        >  1:2841479/1‑140 (MQ=255)
                                                                                                                   agagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTGTTCACTTCCGCTTTCAAAATGGCCCTGGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                        <  1:1892886/140‑1 (MQ=255)
                                                                                                                   agagGCTCTCTAATTACTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                        <  1:30463/140‑1 (MQ=255)
                                                                                                                    gagGCTCTCTATTTATTTTATAGATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCTAGAGGTGGGTTTGCTGAGGCCTGTGCTGCGGCGGCGa                       >  1:3768012/1‑140 (MQ=255)
                                                                                                                    gagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcgt                       >  1:1655051/1‑139 (MQ=255)
                                                                                                                    gagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGa                       <  1:3429736/140‑1 (MQ=255)
                                                                                                                    gagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGa                       >  2:5035141/1‑140 (MQ=255)
                                                                                                                    gagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGa                       >  1:766557/1‑140 (MQ=255)
                                                                                                                     agGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAg                      <  2:3948654/140‑1 (MQ=255)
                                                                                                                     agGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAAGCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAg                      <  1:2314499/140‑1 (MQ=255)
                                                                                                                        ctctctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAACATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                   <  2:2255889/140‑1 (MQ=255)
                                                                                                                        ctctctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                   <  1:1205743/140‑1 (MQ=255)
                                                                                                                        ctctctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                   <  1:4248165/140‑1 (MQ=255)
                                                                                                                        ctctctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                   >  2:1071216/1‑140 (MQ=255)
                                                                                                                        ctctctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGCCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                   <  2:3293774/140‑1 (MQ=255)
                                                                                                                        ctctctAATTATTTTATACATTTTAGAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                   <  2:2920869/140‑1 (MQ=255)
                                                                                                                           tctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAg                >  2:5091567/1‑140 (MQ=255)
                                                                                                                             tAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCGCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGAt              <  1:3080803/140‑1 (MQ=255)
                                                                                                                               attattTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTg            <  1:1589688/140‑1 (MQ=255)
                                                                                                                                  attTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTa         >  1:4504566/1‑140 (MQ=255)
                                                                                                                                  attTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTa         >  2:3634526/1‑140 (MQ=255)
                                                                                                                                  attTTATACATTTTATAAGTGCGGCCCAATCCAAGTCACCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTa         <  2:1810363/140‑1 (MQ=255)
                                                                                                                                  agtttATACATTTTCTAAGTGCGGCCCAATCCAAGTCGCCGAAGCCTATTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTa         <  2:2055508/138‑1 (MQ=255)
                                                                                                                                     ttATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATcc      >  2:1370746/1‑140 (MQ=255)
                                                                                                                                     ttATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATcc      >  2:4235964/1‑140 (MQ=255)
                                                                                                                                     ttATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATcc      >  2:4013242/1‑140 (MQ=255)
                                                                                                                                       ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt    <  2:4299252/140‑1 (MQ=255)
                                                                                                                                       ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt    <  1:2072053/140‑1 (MQ=255)
                                                                                                                                       ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt    <  1:4760457/140‑1 (MQ=255)
                                                                                                                                       ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt    <  1:3166036/140‑1 (MQ=255)
                                                                                                                                       ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt    <  2:1507235/140‑1 (MQ=255)
                                                                                                                                       ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt    <  1:3938521/140‑1 (MQ=255)
                                                                                                                                       ataCATTTTATAAGTGCGGCCCAATCCAACTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt    <  1:3531731/140‑1 (MQ=255)
                                                                                                                                         aCATTTTTTAAGTGCGGCCCAATCCAAGTCTCCGAAGCATTTTTAAATTCCGTTTTCAAATTGGCCCTTGGCAAGCTCGGCAAGAGTTAGGTTTGCAGAGGCATGTTATGCGGCGGCGAGTGCCAGATTGTTATCCTTCa  <  2:1305586/140‑1 (MQ=255)
                                                                                                                                         aCATTTTATAAGTGCGGCCCAATCCACGTCTCCGAAGCCTTTTTCATTTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCa  <  2:1718504/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TGCAATCACAGATCTGAAAAAACAAGGCTACACATTTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCA  >  NZ_CP034943/847096‑847372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: