Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 986,786 C→A 10.0% Q367K (CAA→AAA)  spoVR → stage V sporulation protein SpoVR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943986,7860CA10.0% 705.3 / 23.8 273Q367K (CAA→AAA) spoVRstage V sporulation protein SpoVR
Reads supporting (aligned to +/- strand):  ref base C (113/124);  new base A (2/33);  total (115/158)
Fisher's exact test for biased strand distribution p-value = 5.75e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

AGAAATGAAGAAAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCT  >  NZ_CP034943/986647‑986924
                                                                                                                                           |                                                                                                                                          
   aaTGAAGAAAATTGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCaaa                                                                                                                                         >  2:4147772/1‑140 (MQ=255)
       aagaaAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACa                                                                                                                                     <  2:4047134/140‑1 (MQ=255)
         gaaAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAgg                                                                                                                                   >  2:213519/1‑140 (MQ=255)
         gaaAATGGGAGTGAAGCCTGGCTAAGGCATTGAGAAAATGTATGAGGACAGGGAAATTGAATCCGATATCTCTTTCATACCCAATTAATTAAAAAAACACTAAGTTATACCCGAGCATCTTTAATTGATTCAAAAACAgg                                                                                                                                   >  2:3170827/1‑140 (MQ=255)
           aaaTGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGCGGACAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGGTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGc                                                                                                                                 <  2:1299266/140‑1 (MQ=255)
            aaTGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCa                                                                                                                                <  2:957701/140‑1 (MQ=255)
            aaTGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCa                                                                                                                                <  2:2235316/140‑1 (MQ=255)
            aaTGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCa                                                                                                                                <  2:4101111/140‑1 (MQ=255)
             atcggactGAAGCCTGTCGCAGGCAGTGAGAAACTGTTTGAGGTCAGGGAACTTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCTAGGATCTTTATTTGTTTCAAAAACAGGGCag                                                                                                                               <  2:3023506/133‑1 (MQ=255)
             aggggCGTGAAGCCTGGCTCAGGCAGGGAGTAACTGTTTGAGGGCAGGGAAATTGACTCCGATATCTCTTTCAGCCGCAATGACTTAACAAAACACTGAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCag                                                                                                                               <  2:1676689/138‑1 (MQ=255)
             aTGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCag                                                                                                                               <  2:4091968/140‑1 (MQ=255)
              tGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCaga                                                                                                                              <  1:4670875/140‑1 (MQ=255)
              tGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCaga                                                                                                                              <  1:5140049/140‑1 (MQ=255)
              tGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCaga                                                                                                                              <  1:4207713/140‑1 (MQ=255)
              tGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCaga                                                                                                                              <  2:3339014/140‑1 (MQ=255)
                 gCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                           >  1:488125/1‑140 (MQ=255)
                 gCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGGCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGAGTTCGGTATGCGCGGGGCTCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                           >  1:1781683/1‑140 (MQ=255)
                    tGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATtat                                                                                                                        >  1:1662254/1‑140 (MQ=255)
                           tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTCGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                 >  1:559109/1‑140 (MQ=255)
                           tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCaaaaa                                                                                                                                       >  2:484461/1‑118 (MQ=255)
                           tGGCTCAGGCAGGGAGAAAATGGTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGAGCGAGGATCTTTATTTGTTTCAAAAACAGTGCAGAGATTATAAAAtcg                                                                                                                 >  1:1892978/1‑140 (MQ=255)
                                 aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                           >  1:577419/1‑140 (MQ=255)
                                 aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATATTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                           <  1:4492641/140‑1 (MQ=255)
                                       ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                     >  2:1432697/1‑140 (MQ=255)
                                        gagaAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATg                                                                                                    >  2:2740911/1‑140 (MQ=255)
                                        gagaAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATg                                                                                                    >  2:1941031/1‑140 (MQ=255)
                                             aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTAAAAAACAGGGCAGAGATTATAAAATAGTCTACAAAGAATGGAAAt                                                                                               >  2:635393/1‑140 (MQ=255)
                                              aTGTTTGAGGTCAGGTAAATTGAATCCGATATCTCTATCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATATTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATc                                                                                              <  2:1218607/140‑1 (MQ=255)
                                                 tttGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGt                                                                                           >  1:2158016/1‑140 (MQ=255)
                                                   tGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTAc                                                                                         >  1:1006332/1‑140 (MQ=255)
                                                   tGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTAc                                                                                         >  2:1842047/1‑140 (MQ=255)
                                                      ggTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAACAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTAcgcg                                                                                      <  2:885745/140‑1 (MQ=255)
                                                      ggTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTAcgcg                                                                                      <  2:2158759/140‑1 (MQ=255)
                                                        tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  2:3828782/140‑1 (MQ=255)
                                                        tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  2:1410134/140‑1 (MQ=255)
                                                          aGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCa                                                                                                                                <  1:4747549/94‑1 (MQ=255)
                                                          aGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCa                                                                                                                                >  2:4747549/1‑94 (MQ=255)
                                                                   gAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAg                                                                         <  2:1643554/140‑1 (MQ=255)
                                                                     aTCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAACGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCa                                                                       <  1:5071753/140‑1 (MQ=255)
                                                                       ccGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATg                                                                     <  1:3830309/140‑1 (MQ=255)
                                                                           tatCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCggg                                                                 <  2:2012155/140‑1 (MQ=255)
                                                                           tatCTCTTTCATCCGCAAGTACTTAACAAAAGACTTAGTTAAGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCggg                                                                 <  2:838788/140‑1 (MQ=255)
                                                                              ctctTTCATCCGCAATTACTTAACAAAAGACGGAGTGATGCGCGAGGATCTTTATGTGTTTCAAAAACAGGGCAGAGAGGATAAAAGCGTCGACAAAGAATGGACATCGGTACGCGACCAGCTCGTGAGCGTGCGGGTac                                                              >  1:2385529/1‑139 (MQ=255)
                                                                                   tCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAcggc                                                         >  2:3560858/1‑140 (MQ=255)
                                                                                   tCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAcggc                                                         >  2:4781380/1‑140 (MQ=255)
                                                                                      tCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCggg                                                      >  1:1112387/1‑140 (MQ=255)
                                                                                       ccGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGt                                                     >  2:4413391/1‑140 (MQ=255)
                                                                                         gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  1:991893/107‑1 (MQ=255)
                                                                                         gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                   >  1:3157890/1‑140 (MQ=255)
                                                                                         gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                   <  1:2274216/140‑1 (MQ=255)
                                                                                         gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                   <  1:1825326/140‑1 (MQ=255)
                                                                                         gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                   <  2:4870358/140‑1 (MQ=255)
                                                                                           aaTTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTcc                                                 >  1:2488895/1‑140 (MQ=255)
                                                                                           aaTTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCGCGTGAGCATGCCGGTAAACGGCGGGTTTcc                                                 >  2:3793217/1‑140 (MQ=255)
                                                                                                 ttAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATAttt                                           >  1:3276895/1‑140 (MQ=255)
                                                                                                   aaCAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATAGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                         >  2:5182323/1‑140 (MQ=255)
                                                                                                   aaCAAAAGACTTAGTTATGCGCGAGGATCTTTATTTCTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTgt                                         <  2:1096096/140‑2 (MQ=255)
                                                                                                     cAAAAGACTTAGTTATGCGCGGGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                       <  2:1781683/140‑1 (MQ=255)
                                                                                                        aaGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                       >  1:3059285/1‑137 (MQ=255)
                                                                                                               ttttATGCGCGAGTATTTTTTTTTTTTTCAAAAACAGGGCAGATATTATAAACACGGCGACAAATAATGCAAATCGGGCCGCTACCAGCTCGGGAGCATGGGGGTAAACGGCGGTTTGCCATATTTGACAGTAAacgacg                             <  1:4007316/138‑1 (MQ=255)
                                                                                                               aGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                         >  2:1088327/1‑128 (MQ=255)
                                                                                                               aGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAacgacg                             >  1:2933932/1‑140 (MQ=255)
                                                                                                               aGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAATAAacgacg                             >  2:3621034/1‑140 (MQ=255)
                                                                                                                    tGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGAt                        >  1:4370144/1‑140 (MQ=255)
                                                                                                                    tGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGAt                        >  1:2080208/1‑140 (MQ=255)
                                                                                                                    tGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGAt                        >  1:3010400/1‑140 (MQ=255)
                                                                                                                      cgcgTGGTTATTTATTTTTTTAAAAAACAGTGAAGAGATTATAAAATCGTCTAAAAAGAAGGTAATTCGTTACGCGACCAGCTCGTGAGCTTGGGGTTAAACGGGGGTTTTCAATATTTGACAGTACACGACGGTGATTa                      <  1:1314694/140‑1 (MQ=255)
                                                                                                                      cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGCGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTACACGACGGTGATTa                      >  2:2330374/1‑140 (MQ=255)
                                                                                                                      cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                      >  1:4303320/1‑140 (MQ=255)
                                                                                                                      cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                      >  1:2718536/1‑140 (MQ=255)
                                                                                                                       gcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTAc                     >  2:470791/1‑140 (MQ=255)
                                                                                                                       gagAGGATCTTTATTTGTTTAAAAAACTGTGCTCATTTTATAAACTCGTCCACAAAGAATGTAAATGGGTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTGTCCATATTTGAAAGTAAACGACGGTGATTAc                     <  2:3914080/138‑1 (MQ=255)
                                                                                                                          aGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATg                  >  2:2840691/1‑140 (MQ=255)
                                                                                                                                 ttATTTGTTACAAAACCAGGCCAGATATAATAAAATCGGCGACAAAGACTGGAAATGGGTACGCGACCAGCTGGTGAGCTGGCGGTTAAACGGCGGTTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATa           <  1:3352886/140‑1 (MQ=255)
                                                                                                                                   tttttttTCAAAAAATGGGCAGTCATTTTAAAAGCGTCTACAATGAATGGAAAGCGGTCCGGGACCAGGTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACATTACACGACGGTGATTACATGAAAAATAAc         <  1:3667185/139‑1 (MQ=255)
                                                                                                                                   tttttttACAAAAACCGGGAAGCCATTATAACACGTTCGAAAAACAATGGAAATCGGCCCGCGACCAGCACGTGCGCAGGGGGGTAACCGGCGGGTTTCAATATTTGACAGAAAACGACGGTGATTACATGAAAAATAAc         <  1:707240/139‑1 (MQ=255)
                                                                                                                                   ttttGTGTCAAAAACAGGGCCGAGATTTTAAAAGCGTCGACAAATAAGGGAAATCGGTACGCGACCTGCGGGTGCGAATGGGTGTAAACGGGGGGTTTCCATATTTGACAGTACACGACGGTGATTACATGAAAAATAAc         <  2:709247/139‑1 (MQ=255)
                                                                                                                                   aTTTTTTACAAAAACTGGGCAGAGTTTAAAAAATCGTCGAAAAAGAATGTAAATCGGTCCGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  1:1041000/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTCAAAAACAGGGCAGCGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTTAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:1127622/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCTTGCGGGTAAACGGCGGTTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:2264208/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTAAAAAACAGGGCAGATATTATAAAAGCGTCGACAAAGAATGTAAATCGGACCGCGACCAGCTCGTCAGAATGCGGGAAAACGGCGGGTTTCCATATTTCACAGTAAACGACGGTGATTACATGAAAAATAAc         <  1:880932/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTAAAAAACAGGCCAGAGTTTATAAAACCGTCGACAAAGAATGGAAATCGGTACGCCACCAGATCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:4606324/140‑1 (MQ=255)
                                                                                                                                       ttttAAAAACCCGGCCAGAGTTTATAAAATGGTCCACAACTATTGGAAATGGGTACGCGACCAGCTCGTTCGCAGGCGGGTAAAGGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc     <  1:4899062/139‑1 (MQ=255)
                                                                                                                                       gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAga     >  2:3252888/1‑139 (MQ=255)
                                                                                                                                       gTTTAAAAAACAGGGCATATATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc     <  2:3145552/140‑1 (MQ=255)
                                                                                                                                        tttCAAAAACAGGGCATCTATTTTAAAATCTTCGACAAAGATTGGAAATCGGTACGCGACCAGATCGTGAGCATGCGGGTAAAGGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:5100721/140‑1 (MQ=255)
                                                                                                                                        tttCAAAAACAGGGCAGATATTATAAAATCTTCTAAAAAGAATGGAAATCGTTACGCGACCAGATCGTGAGAATGCGGGTAAACGGCGGTTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:2307851/140‑1 (MQ=255)
                                                                                                                                        tttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGATTGCAAATCGGTACGCGACCAGCTGGTGAGAATGCGTGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:3466764/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACTGGGCAGATATTATAAACTCGTCCAAAAACTATGGAAATCGGTACGCCACCAGCTCGTGAGAATGCGGGTAAACGGGGGGTTTCAATTTTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:3733427/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACTGGGCAGAGTTTATAAAATCGTCGACAAAGATTGGAATTCGGTACGCTACCAGCTCGTGAGCATGCGGGTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:2457964/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGTCACAGATTATAAAATCTTCGACAAAGATTGTAAATCGGTACGCGACCAGCTCGTGAGCTTGCGGTTAAACGGCGGTTTTCCATTTTTGACAGTAAACGACTGTGATTACATGAAAAATAACGAGct    <  2:3672/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGGCAGATATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGTTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:4999140/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGGCAGAGATTATAAAATCGTCGAAAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGGGGGTAAACGGCGGTTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:2943770/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGGATGAGATTATAAAATCTTCGACAAACAATGTAAATCGGTACGCCACCAGATCGTGAGCTTGCGGTTAAACGGCGGGTTTCCATATTTGACTTTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:3589713/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGGAAGAGATTATAAAAGCGTCGACAAATATTGCAATTCGGTACGCGCCCAGCTGGTGAGCAGGGGGGTAAACGGGGGTTTTCAATATTTGACAGTACACGACGGTGATTACATGAAAAATAACGAGct    <  1:391185/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGCCATACATTATAAAATCTTCTACAAAGAATGGAAATCGGTACGCGACAAGCTCGTGAGAATGGGGTTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:4771099/140‑1 (MQ=255)
                                                                                                                                        tgacAAAAACAGGGCAGAGATTATAAACGCGCCGACAAAGAGGGCAAACGGGGACGCGACCAGCTCGTTAGCAGGCGGGTAAACGGCGGTTTTCCATATTGGACAGGAACCGACGGTGATTACATGAAAAATAACGAGct    <  2:4916054/137‑1 (MQ=255)
                                                                                                                                         ttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    >  1:2554776/1‑139 (MQ=255)
                                                                                                                                         ttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:2554776/139‑1 (MQ=255)
                                                                                                                                          tCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGctct  >  1:2952502/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGAAATGAAGAAAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCT  >  NZ_CP034943/986647‑986924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: