Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,097,430 C→G 8.7% S58C (TCT→TGT)  EO946_RS05675 → MarR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,097,4300CG 8.7% 600.6 / 2.3 233S58C (TCT→TGT) EO946_RS05675MarR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (118/87);  new base G (4/11);  total (122/111)
Fisher's exact test for biased strand distribution p-value = 2.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAA  >  NZ_CP034943/1097291‑1097568
                                                                                                                                           |                                                                                                                                           
taaTTATATGCGGAGCTCATTCATCAGCAGCAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGTCGAATTGAAGAAGCTCTATGTCGAGCTCGGGAGTAAATCTCAAGTATCGATGACAGAAATTCATGttctttc                                                                                                                                             <  2:1723901/139‑1 (MQ=255)
  aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                                                           <  1:2886346/140‑1 (MQ=255)
  aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                                                           <  1:1214675/140‑1 (MQ=255)
  aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                                                           <  2:4089464/140‑1 (MQ=255)
  aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                                                           <  1:1931022/140‑1 (MQ=255)
  aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                                                           <  2:2381802/140‑1 (MQ=255)
        tGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGACTTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTg                                                                                                                                     >  1:5022284/1‑140 (MQ=255)
        tGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCTATGACAGAAATTCATGTTCTTTCTTGTGTTg                                                                                                                                     >  2:1061769/1‑140 (MQ=255)
        tGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTg                                                                                                                                     >  2:1867424/1‑140 (MQ=255)
        tGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTg                                                                                                                                     >  1:1709097/1‑140 (MQ=255)
         gCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTATAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTgg                                                                                                                                    <  2:1888414/140‑1 (MQ=255)
          cGGAGCTCATTCATCAGCAGGAGAACTGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTggg                                                                                                                                   <  1:1021639/140‑1 (MQ=255)
          cGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTggg                                                                                                                                   <  2:1127566/140‑1 (MQ=255)
          cGGAGATCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTggg                                                                                                                                   <  2:4652139/140‑1 (MQ=255)
           ggAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTgggg                                                                                                                                  >  1:4865253/1‑140 (MQ=255)
           ggAGCTCATTCATCAGCAGGAGAAAGGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTgggg                                                                                                                                  >  1:3364226/1‑140 (MQ=255)
             aGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGAc                                                                                                                                >  2:4442629/1‑140 (MQ=255)
              gCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                                              <  2:822179/140‑1 (MQ=255)
              gCTCATTCAGCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAATCTCGATGTCGAGCACGGGAGTAAATCTCAAGTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                                              <  1:505082/140‑1 (MQ=255)
                tcattcatCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACact                                                                                                                            >  2:4007280/1‑138 (MQ=255)
                 cattcatCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATg                                                                                                                           <  1:3452059/140‑1 (MQ=255)
                   ttcatCAGCAGGAGAACTGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGaa                                                                                                                         <  2:536846/140‑1 (MQ=255)
                        cagcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaat                                                                                                                    <  2:3385778/140‑1 (MQ=255)
                         agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTTTGTTGGGGACAATTAACcaatc                                                                                                                   >  2:1249814/1‑140 (MQ=255)
                         agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatc                                                                                                                   >  1:4834876/1‑140 (MQ=255)
                              gagaCATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                              <  2:4848813/140‑1 (MQ=255)
                              gagaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                              >  2:894915/1‑140 (MQ=255)
                              gagaAATGGGAGGGGCTCATTAAAGCCTTGGTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                              <  2:2518255/140‑1 (MQ=255)
                               agaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTc                                                                                                             <  2:4352577/140‑1 (MQ=255)
                                gaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCa                                                                                                            >  1:5185068/1‑140 (MQ=255)
                                        aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                    >  1:3837176/1‑140 (MQ=255)
                                         ggggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGATCACGTGAGTAAATCTCAATTATCTATGAAAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTAACGTCTATTg                                                                                                   >  2:3173295/1‑140 (MQ=255)
                                          gggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGc                                                                                                  >  2:3404548/1‑140 (MQ=255)
                                          gggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGc                                                                                                  >  2:1467574/1‑140 (MQ=255)
                                          gggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTACTTTCTTGTGTTGGGGACAATGAAACAATCAATGTCACGTCTATTga                                                                                                  >  1:2972402/1‑139 (MQ=255)
                                            gCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTGTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAg                                                                                                >  2:1738801/1‑140 (MQ=255)
                                              tCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaa                                                                                              <  1:4442629/140‑1 (MQ=255)
                                               cATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaa                                                                                             >  1:1409211/1‑140 (MQ=255)
                                               cATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaa                                                                                             <  1:2009059/140‑1 (MQ=255)
                                                 ttAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaaaa                                                                                           >  1:2323942/1‑140 (MQ=255)
                                                      gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                      >  2:31085/1‑140 (MQ=255)
                                                      gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                      >  2:1826048/1‑140 (MQ=255)
                                                        cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                    <  1:3124632/140‑1 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTGTATTGCAGAAAAAATGAATACg                                                                                  >  2:2825679/1‑140 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                  <  2:4534106/140‑1 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                  >  1:4686072/1‑140 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                  >  1:416308/1‑140 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                  >  2:3588288/1‑140 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                  >  1:738052/1‑140 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                  >  1:213272/1‑140 (MQ=255)
                                                          ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCAAGTCTATTGCAGAAAAAATGAATACg                                                                                  >  2:3954961/1‑140 (MQ=255)
                                                          ttttGGCTCACGAAGTGAAGAAGCTAGATGTCGAGCACGGGCTTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGACCCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                  >  1:2422928/1‑140 (MQ=255)
                                                             tGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCATAAAAAATGAATACGACa                                                                               <  2:3730800/140‑1 (MQ=255)
                                                                    tgaattgaattAGCTCGATTTCTAGAACGGTATTAAATCTAAATTATCGTTGACATAATTTAATTTTCTTTTTTGTTTTGGGTACAATGAACCAATAATTTTCACGTCTTTTGCAGAAAAAATGAATACGAAAAAAGCGa                                                                        <  2:3802269/139‑1 (MQ=255)
                                                                    cGAATTTAAGAAGGTCGATGTCGAGCACGGGAGTAAATCTCAAGTAGCGTTGACAGAATTTCTTTTTCTTTCTTGTGTGGGGTACAATGAACCAATAAATGTAAGGTCTATTGCACAAAAAATTAATCCGACCAAAGCGa                                                                        <  2:4801610/140‑1 (MQ=255)
                                                                         tGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTa                                                                   >  1:4620979/1‑140 (MQ=255)
                                                                                 cttgttGTCGAGCCGGGGAGTAAATCTCAATTTTCGATCACATAAATTAATTTTTTTTGTTGTTTTGGGCACAATTAACAAATCAATGTCACGTCTTTTCAAGAAAAAATCAATACCACAAAAGCGACTGTATCGCGCAt                                                           <  2:3313784/135‑1 (MQ=255)
                                                                                 ctggttGTCGAGCACGGTAGTAAATCTAAATTATCGATGAAATAAATTAATTTTTTTTCTTGTTTTGGGCAAAATCAACAAATAAATTTCACGTCTATTGAAGAAAAAATGAATACCAAAAAAGCGACTGTATCGCGCAt                                                           <  2:203428/135‑1 (MQ=255)
                                                                                 ctctATGTCGAGCAGGGGATAAAATATAAATTAGCGATCACACAATTTCTTGTTCTTTCTTGTGTTGGGTACAATCAACCAATCAATGTCACGTATATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCAt                                                           <  2:3509086/140‑1 (MQ=255)
                                                                                 cTCGTTGTGGAGCACGGGATTAAATCTAATTTATCTATGACAGAATTTATTTTTCTTTGTTGTTTTGGGTAAAATTAACAAACCAATGGCACTTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCAt                                                           <  2:4782593/140‑1 (MQ=255)
                                                                                      tgtctcgCACGGTATTACATCTCATTGAGCGATCACAGACATTCTTGGTGTTTCTTTGGGGGGGTCAAATGAACCATTCATTGTCCGGTCTATTGAAGAAAATATGAATCCGAAAAAAGCGACTGTATCGCGCATCAGca                                                      <  2:2632640/134‑1 (MQ=255)
                                                                                           aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                              <  2:2766807/79‑1 (MQ=255)
                                                                                           aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                 >  1:683080/1‑140 (MQ=255)
                                                                                           aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                 >  1:2850403/1‑140 (MQ=255)
                                                                                           aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                 >  1:4802118/1‑140 (MQ=255)
                                                                                           aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                 >  1:3097333/1‑140 (MQ=255)
                                                                                           aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                 >  1:3503433/1‑140 (MQ=255)
                                                                                           aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                 >  1:1876175/1‑140 (MQ=255)
                                                                                                  gAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCg                                          >  1:80914/1‑140 (MQ=255)
                                                                                                       aaTCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGcgcg                                     >  2:806792/1‑140 (MQ=255)
                                                                                                       aaTCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGcgcg                                     >  1:1488603/1‑140 (MQ=255)
                                                                                                       aaTCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGcgcg                                     >  1:1546892/1‑140 (MQ=255)
                                                                                                            cAATTATCGATGACAGAAATTCATGTTCTTTGTTGTGGTGGGGCCAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttg                                >  1:2578150/1‑139 (MQ=255)
                                                                                                            cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                >  1:1986866/1‑140 (MQ=255)
                                                                                                            cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                >  1:344074/1‑140 (MQ=255)
                                                                                                            cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                >  1:2212202/1‑140 (MQ=255)
                                                                                                            cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGGCTATTGCAGAAAAAATGAATACGACAACAGCGACTGTATCGCGCATCAGCACAATATTGCTCGGCGCGGGttt                                >  1:3283368/1‑140 (MQ=255)
                                                                                                             aaTTATCTATGACATAATTTCTTGTGATTTTTTGTGTTGGGGACAATCAACCAATCAATGTCACGTATTTTGCATAAAAAATTAATACGCCAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                               <  1:1093280/140‑1 (MQ=255)
                                                                                                             aaTTATCGTTTACATAAATTCATTTTCTTTCTTGTGTTGGGTAAAATCAAACAATAAATTTCACTTCTATTCCACAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                               <  2:5033805/140‑1 (MQ=255)
                                                                                                             aaTTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCCTCAGCACAAAATTGCTCGGCGCGGGTTTc                               >  2:5012655/1‑140 (MQ=255)
                                                                                                             aaTTATCGATGAAAGAAATTCAGTTTCTTTCTGGTGTGGGGGACAATGAAACAATCAAGGTCACGTATATTGCATAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                               <  2:55240/140‑1 (MQ=255)
                                                                                                               ttATCGATGACATAATTTATTGTTTTTTCTTGTGTTGGGGAAAATGAACAAATCAATGTCACGTCTTTTGCACAAAAAATTAAAACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                             <  1:894915/140‑1 (MQ=255)
                                                                                                                  tCGATGACAGAAATTCTTGTTCTTTGTTGTGTTGGGGACAATGAAACAATCATTGTCACGTCTTTTGAAGAAAAAATTAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                          <  1:3588288/140‑1 (MQ=255)
                                                                                                                  tCGATCACATAATTTATTTTTTTTTGTTGTTTTGGGGAAAATTAACAAATAAATGTCAGGTATATTGCACAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                          <  1:4214921/140‑1 (MQ=255)
                                                                                                                  gctaTGACACAATTTATTGTTGTTTTTTGTGTTGGGCACAATCAACCAATCATTGTCACGTCTTTTGCACAAAAAATGAATCCCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                          <  1:4566214/137‑1 (MQ=255)
                                                                                                                       gaaataaaTTATTGTTCTTTCTTTTGTTGGGCACAATGACACAATCATTGTCAGGTATATTGCAGAAAAAATTAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                     <  1:1374013/135‑1 (MQ=255)
                                                                                                                       gaaataaaTTAATTTTCTTTCTTGTGTTGGGCACAATCAACAAATAAATGTCACTTATTTTCCATAAAAAATGAATCCCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                     <  1:271162/135‑1 (MQ=255)
                                                                                                                       gACATAAATTCATGTGATTTCTTGTGTTGGGCAAAATCAACCAATCAAGGGCAGTTATATTGCATAAAAAATGAATACCCCACCCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                     <  1:1609032/140‑1 (MQ=255)
                                                                                                                       gACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                     >  1:4632288/1‑140 (MQ=255)
                                                                                                                       cacacAAATCCCTGTTCGTTCTTGGGTGGGCCCCAAACAACCAACCAATGCCACGCCTATCCCACAAAAAACCACTCCCCCACACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                     <  1:2750390/139‑1 (MQ=255)
                                                                                                                         cataatttCTTGTTCTTTCTGGTTTTGGGCACAATTAACCAATCATGTTCACTTCTTTTCCAGAAAAAATAAAAACCAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                   <  1:1146105/134‑1 (MQ=255)
                                                                                                                         cataaaTTCATTTTGTTTCTTGTGTGGGGGACAATCAACCAATCAATGTCACGTCTATTGCACAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                   <  2:242043/137‑1 (MQ=255)
                                                                                                                         aataaaTTAATGTTATTTCTTTTTTTGGGCACAATGAACAAATCAATGTCACGTATATTGAAGAAAAAATGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                   <  2:3893505/137‑1 (MQ=255)
                                                                                                                         aataaaTACAGGTTTTTTCTTGTGTTGGGCACAATGAACCAATCAATGTCAGTTTTATTCCATAAAAAATTAATACTAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                   <  2:1622989/137‑1 (MQ=255)
                                                                                                                            aaaTTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAg                >  1:4370528/1‑140 (MQ=255)
                                                                                                                                    tttgtttctgGTTTTGGGGAAAATTAACCAATAAATGTCACTTTTATTGCAGAAAAATTTAATACTCCAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa        <  2:460249/136‑1 (MQ=255)
                                                                                                                                    gtgctttcttGTGTGGGGGACAATGAACCAATCAATGTCAGGTCTATTGCAGAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa        <  2:4361222/137‑1 (MQ=255)
                                                                                                                                    gTTTTTTCTTGTGTTGGGGAAAATTAACAAATCAATGTCACTTATTTTGAACAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa        <  1:3954961/140‑1 (MQ=255)
                                                                                                                                    gTTCTTTGTTGTGTTGGGCACAAGCAACCAATCAATGTCACGTATTGTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa        <  1:1425850/140‑1 (MQ=255)
                                                                                                                                    gTTCTTTGTGGTGTTGTGCCAAATTAACAAATCATTTTCACGTTTATTCAATAAAAAATTAATCCGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa        <  1:3924822/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAA  >  NZ_CP034943/1097291‑1097568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: