Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,097,464 G→T 7.6% T69T (ACG→ACT EO946_RS05675 → MarR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,097,4640GT 7.6% 518.0 / 2.0 217T69T (ACG→ACTEO946_RS05675MarR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (122/67);  new base T (2/26);  total (124/93)
Fisher's exact test for biased strand distribution p-value = 4.46e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

AGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGA  >  NZ_CP034943/1097331‑1097603
                                                                                                                                     |                                                                                                                                           
aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                                       >  1:1126731/1‑140 (MQ=255)
aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                                       >  1:3837176/1‑140 (MQ=255)
aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                                       >  1:3105232/1‑140 (MQ=255)
  gggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGc                                                                                                                                     >  2:1467574/1‑140 (MQ=255)
  gggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGc                                                                                                                                     >  2:3404548/1‑140 (MQ=255)
  gggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTACTTTCTTGTGTTGGGGACAATGAAACAATCAATGTCACGTCTATTga                                                                                                                                     >  1:2972402/1‑139 (MQ=255)
       cATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaa                                                                                                                                >  1:1409211/1‑140 (MQ=255)
       cATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaa                                                                                                                                <  1:2009059/140‑1 (MQ=255)
              gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                                         >  2:31085/1‑140 (MQ=255)
              gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                                         >  2:1826048/1‑140 (MQ=255)
                cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                                                       <  2:4834876/140‑1 (MQ=255)
                cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                                                       <  2:2791377/140‑1 (MQ=255)
                cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                                                       <  2:1877421/140‑1 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTGTATTGCAGAAAAAATGAATACg                                                                                                                     >  2:2825679/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  1:1874579/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  1:1927093/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  1:1971274/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  2:4436886/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  1:4490393/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  2:256365/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  1:1285712/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  1:483966/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCGATTGCAGAAAAAATGAATACg                                                                                                                     >  2:4087034/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCAAGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  2:3954961/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCGTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                     >  1:1432314/1‑140 (MQ=255)
                  ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATAGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAAGACg                                                                                                                     >  1:927893/1‑140 (MQ=255)
                            ctattttaagaagCTCGTTTTCGAGCACGTTAGTAAATCTCAATTATCGTTGAACTAAATTCATGTTCTTTCTTGTGTTGGGGAAAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATCAATACGACAAAAGCGa                                                                                                           <  2:4700273/133‑1 (MQ=255)
                                 tGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTa                                                                                                      >  1:4620979/1‑140 (MQ=255)
                                         ctggttGTCGAGCACGGTAGTAAATCTAAATTATCGATGAAATAAATTAATTTTTTTTCTTGTTTTGGGCAAAATCAACAAATAAATTTCACGTCTATTGAAGAAAAAATGAATACCAAAAAAGCGACTGTATCGCGCAt                                                                                              <  2:203428/135‑1 (MQ=255)
                                         cTCGTTGTGGAGCACGGGATTAAATCTAATTTATCTATGACAGAATTTATTTTTCTTTGTTGTTTTGGGTAAAATTAACAAACCAATGGCACTTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCAt                                                                                              <  2:4782593/140‑1 (MQ=255)
                                         cTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCAt                                                                                              >  1:503670/1‑140 (MQ=255)
                                                   aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                                    >  1:4802118/1‑140 (MQ=255)
                                                   aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                                    >  1:3503433/1‑140 (MQ=255)
                                                   aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                                    >  1:3148949/1‑140 (MQ=255)
                                                        gggAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTCTTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCt                                                                               >  2:375660/1‑140 (MQ=255)
                                                          gAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCg                                                                             >  1:80914/1‑140 (MQ=255)
                                                               aaTCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGcgcg                                                                        >  2:806792/1‑140 (MQ=255)
                                                               aaTCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGcgcg                                                                        >  1:1547665/1‑140 (MQ=255)
                                                                aTCTAAATTATCGATCAAAAAAATAATTTTTCTTTTTTGTGTGGGGCAAAATCAACAAAACAATGTCACTTCTATTCAAAAAAAAATGAATCCCACAACCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg                                                                       <  1:4678405/140‑1 (MQ=255)
                                                                aTCTAAATTATCCATCACATAAATCCATGTCCTTTCTTGTGTTGGGCACAATCACCCAATCATGGTCACGTCTATTGCACAAAAAATCAACCCCACAAACGCGACGGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg                                                                       <  1:4965210/140‑1 (MQ=255)
                                                                    cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                                   >  1:344074/1‑140 (MQ=255)
                                                                    cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                                   >  1:770136/1‑140 (MQ=255)
                                                                    cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                                   >  1:604898/1‑140 (MQ=255)
                                                                    cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                                   >  1:4355732/1‑140 (MQ=255)
                                                                    cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                                   >  1:3893505/1‑140 (MQ=255)
                                                                    cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGGCTATTGCAGAAAAAATGAATACGACAACAGCGACTGTATCGCGCATCAGCACAATATTGCTCGGCGCGGGttt                                                                   >  1:3283368/1‑140 (MQ=255)
                                                                    cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGCACAATGAACCAATCAATGTCACGTCTATAGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                                   >  1:23767/1‑140 (MQ=255)
                                                                     aaTTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                                                                  >  1:2641398/1‑140 (MQ=255)
                                                                     aaTTATCGATGAAAGAAATTCAGTTTCTTTCTGGTGTGGGGGACAATGAAACAATCAAGGTCACGTATATTGCATAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                                                                  <  2:55240/140‑1 (MQ=255)
                                                                       ttATCTATGACAGAAATTCTTGTTCTTTCTTGTTTTGGGGAAAATCAACAAATAAATGTCACGTCTATTGAACAAAAAATCAAACCCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                                <  1:1532319/140‑1 (MQ=255)
                                                                       ttATCGATGAAAGAATTTCTTTTTCTTTCTGGTGTTGGGCACATTGAACCAAGCAAGGTCACGTCTATGGCAGAAAAATTGAATCCGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                                <  2:3568426/140‑1 (MQ=255)
                                                                          tggATGAAATAAATTCTTGTTCTTTTTTGTGTTGGGGACAATCAACCAATCATTTTCACTTATTTTGCATAAAAAATTAATCCCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                             <  1:1036440/138‑1 (MQ=255)
                                                                          tctaTGACATAAATTCAGTTTGTTTGTTGTTTGGGGCAAAATTAACAAAACAATGTCACGTATAGTGAACAAAAAATGAATACCACACACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                             <  1:4629572/137‑1 (MQ=255)
                                                                          tctaTGACAGAATTTATTGTTCTTTATTGTGTTGGGGACAATCAACAAATAATTGTCAGGTCTTTTGCACAAAAAATGAATCCCACAACCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                             <  1:3086338/137‑1 (MQ=255)
                                                                          tctaTGACAGAAATTAATTTTCTTTTTTGTTTTGGGCAAAATGAACAAATCATTGTAACTTATTTTGCATAAAAAATTAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                             <  1:2909024/137‑1 (MQ=255)
                                                                          tCGATGACAGAAATTCTTGTTCTTTGTTGTGTTGGGGACAATGAAACAATCATTGTCACGTCTTTTGAAGAAAAAATTAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                             <  1:3588288/140‑1 (MQ=255)
                                                                          tCGATCACATAATTTATTTTTTTTTGTTGTTTTGGGGAAAATTAACAAATAAATGTCAGGTATATTGCACAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                             <  1:4214921/140‑1 (MQ=255)
                                                                          actaTGACACAAATTCATGTTCTTTCTTGTGTTGGGCAAAATTAACAAATCAATGTCACTTCTATTGAACAAAAAATCAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                             <  2:4370528/137‑1 (MQ=255)
                                                                            gATGACATAAATTCTTGTTCTTTCTTGTGTTGGGCACAATTAACCAATCATTGTAACGTCTTTTGCATAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTcaca                                                           <  2:1732209/140‑1 (MQ=255)
                                                                               gcgaGAAATTATTGATCTTTCTTGTCTTGGGGCCAATGAACAAATCATTGTCACGTTTATTCAAACAAAATTCAATACCAAACAAGCGACTGTATCGCGCATAAGCACAAAATTGCTCGGCGCGGGGTTCCTTCACAGaa                                                        <  2:881597/137‑1 (MQ=255)
                                                                               gaaataaaTTAATTTTCTTTCTTGTGTTGGGCACAATCAACAAATAAATGTCACTTATTTTCCATAAAAAATGAATCCCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                                                        <  1:271162/135‑1 (MQ=255)
                                                                               gACATAAATTCATGTGATTTCTTGTGTTGGGCAAAATCAACCAATCAAGGGCAGTTATATTGCATAAAAAATGAATACCCCACCCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                                                        <  1:1609032/140‑1 (MQ=255)
                                                                               gACAGAATTTCATTTTTTTTATTGTTTTGGGCAAAATGAACAATTAAATTTCACTTCTATTGAAAAAAAAATAAATACCAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                                                        <  1:2239296/140‑1 (MQ=255)
                                                                               cacacAAATCCCTGTTCGTTCTTGGGTGGGCCCCAAACAACCAACCAATGCCACGCCTATCCCACAAAAAACCACTCCCCCACACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGaa                                                        <  1:2750390/139‑1 (MQ=255)
                                                                                 cataatttCTTGTTCTTTCTGGTTTTGGGCACAATTAACCAATCATGTTCACTTCTTTTCCAGAAAAAATAAAAACCAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                      <  1:1146105/134‑1 (MQ=255)
                                                                                 cataaaTTCATTTTGTTTCTTGTGTGGGGGACAATCAACCAATCAATGTCACGTCTATTGCACAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                      <  2:242043/137‑1 (MQ=255)
                                                                                 cacaaaTTCATGTTATTTCTTTTGTTGGTCAAAATTAACAAAACAATTTAACTTATATTCCACAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                      <  2:4355732/137‑1 (MQ=255)
                                                                                 cAGAAATTCTTGTTCTTTCTTGTGTTGGGGAAAATGAACCAATAAATTTCACGTCTATTGCAGAAAAAATGAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                      <  2:5015143/140‑1 (MQ=255)
                                                                                 aataaaTTAATGTTATTTCTTTTTTTGGGCACAATGAACAAATCAATGTCACGTATATTGAAGAAAAAATGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                      <  2:3893505/137‑1 (MQ=255)
                                                                                 aaGAAATTCATTTTCTTTCTTGTGTTGGTGAAAATTAACCAATAAATTTAACTTCTATTGCTGAAAAATTTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                      <  2:3690053/139‑1 (MQ=255)
                                                                                            tttttttattTTGTGGGGGAAAATGAACAAATAATTGTCAGGTCTTTTGCATAAAAAATCAATACCACACACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                           <  1:2106268/136‑1 (MQ=255)
                                                                                            tttgtttctgGTTTTGGGGAAAATTAACCAATAAATGTCACTTTTATTGCAGAAAAATTTAATACTCCAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                           <  2:460249/136‑1 (MQ=255)
                                                                                            gTTTTTTCTTGTGTTGGGGAAAATTAACAAATCAATGTCACTTATTTTGAACAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                           <  1:3954961/140‑1 (MQ=255)
                                                                                            gTTCTTTTTTGTGTTGGGTACAATTAACAAATCATTGTCACGTCTATTGCACAAAAAATTAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                           <  2:3182112/140‑1 (MQ=255)
                                                                                            gTTCTTTCTTGTTTTGTGGAAAATGAACAAATCATTGTCACGTCTATTGCATAAAAAATGAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                           <  2:4578952/140‑1 (MQ=255)
                                                                                            gTTCTTTCTTGTTTTGGGAAAAATTAACCAATCAATTTCACGTATATGGCACAAAAAATTAATCCCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                           <  1:2170132/140‑1 (MQ=255)
                                                                                               ctttcttGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATaa                                        >  1:2488716/1‑140 (MQ=255)
                                                                                                       tgtTGGGTACATTTAACCAATCAATGTCACGACTTTTGCATAAAAAATTAATCCGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                                <  2:2675890/140‑1 (MQ=255)
                                                                                                       tgtTGGGGAAAATGAACAAATAAATGTAACTTATATTGAACAAAAATTCAAACCCACAACCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                                <  1:3404548/140‑1 (MQ=255)
                                                                                                         ttGGGCAAAATGAAACAATAAATGTCACGTCTTTTCCAAAAAAATTGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAaggag                              <  1:256365/140‑1 (MQ=255)
                                                                                                           ggggAAAATCAACCAATAAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGt                            <  2:3737134/140‑1 (MQ=255)
                                                                                                            gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGCTAACAAAAAGGAGGTc                           >  1:709525/1‑140 (MQ=255)
                                                                                                            gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                           >  1:1797743/1‑140 (MQ=255)
                                                                                                            gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                           >  1:3737134/1‑140 (MQ=255)
                                                                                                            gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                           >  2:1628404/1‑140 (MQ=255)
                                                                                                              gACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACCAAAAGGAGGTCTa                         >  2:1378554/1‑140 (MQ=255)
                                                                                                              gACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTa                         >  2:5106053/1‑140 (MQ=255)
                                                                                                                 aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACtt                      >  1:146941/1‑140 (MQ=255)
                                                                                                                 aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACtt                      >  1:3232964/1‑140 (MQ=255)
                                                                                                                          atcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGAc             >  1:4181344/1‑140 (MQ=255)
                                                                                                                           tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg            >  1:4438213/1‑140 (MQ=255)
                                                                                                                           tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg            >  1:4000900/1‑140 (MQ=255)
                                                                                                                           tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg            >  2:1820888/1‑140 (MQ=255)
                                                                                                                           tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg            >  2:1316122/1‑140 (MQ=255)
                                                                                                                           tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg            >  2:1163718/1‑140 (MQ=255)
                                                                                                                               tGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACgccgg        >  1:863290/1‑140 (MQ=255)
                                                                                                                                    cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg   >  2:3332820/1‑140 (MQ=255)
                                                                                                                                    cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg   >  1:1518389/1‑140 (MQ=255)
                                                                                                                                    cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg   >  2:1044612/1‑140 (MQ=255)
                                                                                                                                     gTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGa  >  1:3025098/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                           
AGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGA  >  NZ_CP034943/1097331‑1097603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: