Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,293,328 G→T 10.7% R134L (CGA→CTA)  EO946_RS06740 → sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,293,3280GT10.7% 358.3 / 7.2 183R134L (CGA→CTA) EO946_RS06740sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base G (92/55);  new base T (2/25);  total (94/89)
Fisher's exact test for biased strand distribution p-value = 5.67e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

TCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAG  >  NZ_CP034943/1293189‑1293466
                                                                                                                                           |                                                                                                                                          
tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                                            <  1:2401339/140‑1 (MQ=255)
tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                                            <  2:2880815/140‑1 (MQ=255)
tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                                            <  2:136308/140‑1 (MQ=255)
tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                                            <  2:4696379/140‑1 (MQ=255)
    gCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATc                                                                                                                                        >  1:4791450/1‑140 (MQ=255)
               tttGAGGCGGACGGACAGGTCACAGAAGAAGACAGAGAGAGAATCCAAGAAGCTTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATTAACGAATCGCTGAtcttct                                                                                                                             <  2:2898486/139‑1 (MQ=255)
                  gAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCgg                                                                                                                          >  1:811332/1‑140 (MQ=255)
                  gAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCgg                                                                                                                          >  1:2558793/1‑140 (MQ=255)
                  gAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCgg                                                                                                                          >  1:690675/1‑140 (MQ=255)
                  gAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCgg                                                                                                                          >  1:1018159/1‑140 (MQ=255)
                  gAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCACTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAATGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCgg                                                                                                                          >  1:2914494/1‑140 (MQ=255)
                    gCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTGAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGgtg                                                                                                                        >  1:3525819/1‑140 (MQ=255)
                        gacggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaa                                                                                                                    >  1:154616/1‑140 (MQ=255)
                         acggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGAtcttct                                                                                                                             >  2:5039184/1‑130 (MQ=255)
                          cggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaaaa                                                                                                                  >  2:4165435/1‑140 (MQ=255)
                              cAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATc                                                                                                              >  2:172007/1‑140 (MQ=255)
                                tttATCACTAGCAGACAGAGCGTCATTCCAATAAGCGTTTTCCGTGTTAACGGGCCGATAACATTAATGGTTTTGGCTGATTTTGGTAGTTTGATTTTTTTATGAACGAATCGCTGATCTTCTGGGTGTAAAAAAATCGa                                                                                                            <  2:5187743/138‑1 (MQ=255)
                                gttATCATAAGAATACAGATAGAGAACCCAAGACGCGTTATCCCTGTTAACGGGCAGATAAAAACAAATGTTTTTGCTGCATAAGTTAGAATGCTTTTCTTATCAACTAATCGCTGATCTTCTCGTTTTAAAAAAATCGa                                                                                                            <  2:2510551/138‑1 (MQ=255)
                                  tATCATAAGAAGACAGAGAGAGACTCCAACAAGCGTTATCCCTGTTAACGGGAATACAAAAATAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACTAATCGCTGATCTTGTCGGTGTAAAAAAATCGACt                                                                                                          <  2:4937227/140‑1 (MQ=255)
                                   aTCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACtg                                                                                                         >  2:4874218/1‑140 (MQ=255)
                                       taataagaCAGAGAGACAATCCACTTAGCTTTATCCCTGTTAACGGCCATATAAAAACAAATTTTTTTGGTGCATAAGTTATAATGCTTTTCTTATTAACTAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCa                                                                                                     <  2:2748433/136‑1 (MQ=255)
                                           aagaCAGAGAGAGAATCCAATAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGGGTAAAAAAATCGAGTGTGCAAACg                                                                                                 >  2:1926783/1‑140 (MQ=255)
                                           aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCTCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCATACg                                                                                                 >  2:2827819/1‑140 (MQ=255)
                                           aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                 >  1:4587063/1‑140 (MQ=255)
                                           aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                 >  1:3908154/1‑140 (MQ=255)
                                           aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                 >  1:1789195/1‑140 (MQ=255)
                                                  agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAATATCGACTGTGCAAACGACCATCa                                                                                          >  2:1524067/1‑140 (MQ=255)
                                                        accccAACACGCGTTACCCCGGTTACCGGGCAGACAAAAATAATTGTTTTGGCTGAATACGGTAGATTGCTTTTCTTATGAACTAATCGGGGATCTTCCCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                                    <  1:1247623/137‑1 (MQ=255)
                                                        aaTCAAACAAGCGTTATCCGTGTTAAGGGGCAGAGAAAACGAAAGTTTTTTGGTGCATACGGTCCAATGCTTTTTTTATGAACGACTCGGTGATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                                    <  2:876619/140‑1 (MQ=255)
                                                         aTCCAAGATGCGTTATCCCTGTTAACGGTCAGATAAAATTAAATGTTTTTGCGGCATAAGGTAGAATGCTTTTTTTATTACCTAATGGATGATGTTCTCGGTTTAAAAAAATCGAATGTGCAAACGACCATCAAACGGGc                                                                                   <  2:3882667/140‑1 (MQ=255)
                                                          tCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACTACCATCAAACGGGCg                                                                                  >  2:3920377/1‑140 (MQ=255)
                                                          tCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCg                                                                                  >  1:3988291/1‑140 (MQ=255)
                                                           ccAAGAAGCTTAATCCCTGTTACCGGGAAGAGAAAAACAAATGTTTTTGCTGCATAAGGTAGACTCCTTTTCTTATGAACTAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGa                                                                                 <  2:2215001/140‑1 (MQ=255)
                                                                cggCGTTATCCCTTTTAACGGGCAGACAAAAACAAATGTTTTTGCTGAATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGAGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTa                                                                            <  2:3360857/138‑1 (MQ=255)
                                                                aagCTTTAGCCGTTTTAACGGGCATATAAAATAAATTTTTTTTGCTGCTTAAGTTTTAATGCTTTTCTTATCAACCAATCGCTTATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTa                                                                            <  2:1661282/140‑1 (MQ=255)
                                                                  gctttATCCCTGTTAACGGGCATACAAAAATAAATGTTTTTGCTGCATAAGGTATATTTCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                                          <  2:4549131/137‑1 (MQ=255)
                                                                  gCGTTATCCCTTTTAACGGGCATACAAAAAGAAATTTTTTTGCGCAATAAGGTTGAATGATTTACTTTTCAACCATTCGCTTATTTTCTCGTTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                                          <  1:2143631/140‑1 (MQ=255)
                                                                  gCGTTATCCCTTTTAACGGCCACAAAAAAAGAATTTTTTTTGCTCAATAAGTTAGAATGCTTTTCTTATCAACTAATCGCTGATATTCTGGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                                          <  1:2752718/140‑1 (MQ=255)
                                                                   cGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAg                                                                         >  1:657904/1‑140 (MQ=255)
                                                                       aTCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGc                                                                     >  1:4979614/1‑140 (MQ=255)
                                                                        tacattttAACGGGAATATAAAAATAAATGTTTTTGCTGCATAATGTATAATTTTTTTATTATGAACTAATCGCTGATTTTATCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCa                                                                    <  2:3943694/134‑1 (MQ=255)
                                                                        tCCGTGTTAAGGGGCATATAAAAATAATTGTTTTTGCTGAATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATGTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCa                                                                    <  1:259871/140‑1 (MQ=255)
                                                                             gTTAACTGGCAGAGAACAAGAAATGTTTTTTCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCTCTGATCTACTAGGTGTAAAAAAATCGACTGTGCAAACGACACTCAAAAGGGCGATCGTAAAGATGCAAAGAc                                                               >  1:2209391/1‑140 (MQ=255)
                                                                             gTTAACGGGCAGATAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGATGATCTTCTCCGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGTGCGATCGTAAAGATGCACAGAc                                                               >  1:1506252/1‑140 (MQ=255)
                                                                             gTTAACGGGCAGAGAAAATGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTGATGAACGAATCGCTGATCTTCTCGGTGTAAAAACATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGGAAAGAc                                                               >  2:557999/1‑140 (MQ=255)
                                                                             gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                               >  1:1521065/1‑140 (MQ=255)
                                                                             gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGAGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAgct                                                               >  1:3360857/1‑138 (MQ=255)
                                                                              ttAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAca                                                              >  2:4262838/1‑140 (MQ=255)
                                                                              ttAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGATGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAca                                                              >  2:4327683/1‑140 (MQ=255)
                                                                                   gggCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagg                                                         >  1:1456012/1‑140 (MQ=255)
                                                                                    ggCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagga                                                        >  1:110745/1‑140 (MQ=255)
                                                                                    ggCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagga                                                        >  1:2816039/1‑140 (MQ=255)
                                                                                        gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCaggctgtc                                                    >  1:3943694/1‑135 (MQ=255)
                                                                                        gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                                                    >  1:1249694/1‑140 (MQ=255)
                                                                                                     gTTTTTGCTGCCTCAGGTAGAATGGTTTTCTTATCAACGAATCGCTTATGTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGc                                       <  2:815248/140‑1 (MQ=255)
                                                                                                      tttttGCTGCATATGGTCTAATGCTTTTCTTAGCACCGAATCGCTGATCTTCTGGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                                      <  1:2395298/140‑1 (MQ=255)
                                                                                                      ttttgGCTCCACAAGTTAGAAGGATTTTCTAAGGAACTAATCGGTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                                      <  1:2949962/140‑1 (MQ=255)
                                                                                                                 tAAGGTATTATTCTTTTCTTATGTCCGAATCCCTGATCTTCTCTGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATGGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                           <  2:3743884/140‑1 (MQ=255)
                                                                                                                 tAAGGTAGACGCCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                           <  2:4994869/140‑1 (MQ=255)
                                                                                                                 aagggTACAATGCTTTTATTATCACCCAATCGATCTTATTCTCGGTGAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                           <  1:1896733/137‑1 (MQ=255)
                                                                                                                        tattgattttCTTATGACCGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCAt                    <  2:3486996/134‑1 (MQ=255)
                                                                                                                         aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGACAGCTTGTCATa                   >  2:3829662/1‑140 (MQ=255)
                                                                                                                         aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                   >  1:897602/1‑140 (MQ=255)
                                                                                                                         aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                   >  1:508242/1‑140 (MQ=255)
                                                                                                                           tGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACg                 >  2:2764816/1‑140 (MQ=255)
                                                                                                                              ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGGTCGCTTGCCGGGAAGCTTGTCATACGttt              >  2:3558075/1‑140 (MQ=255)
                                                                                                                              ttttCTTATGAACGAATCGCTGATCTTCTCGGGGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCTATCGTAAAGATGCAAAGACAGCAGGTGGAAAAGAAGCGATCGATTGCCTGAAAGCTTGTCATACGttt              >  1:2038865/1‑140 (MQ=255)
                                                                                                                               tttGTTTTGAACGAATCGCTGATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg             <  1:4470166/140‑1 (MQ=255)
                                                                                                                               tttCTTATGAACGATTCGAGGATCTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg             <  1:307778/140‑1 (MQ=255)
                                                                                                                               tttCTTATGAACGAATCGCTTATGTTATCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg             <  2:2629176/140‑1 (MQ=255)
                                                                                                                               tttCTTATGAACCACTCGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg             <  2:2855931/140‑1 (MQ=255)
                                                                                                                               tttCTTATCACCGAATCCCTGATCTACTGGGGTTAAAACAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg             <  1:4446043/140‑1 (MQ=255)
                                                                                                                               tttCTTATCAACGAAGCGCTGAACTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg             <  1:963081/140‑1 (MQ=255)
                                                                                                                               tttCTAATGAACGAATCGATGATATTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg             <  1:4269536/140‑1 (MQ=255)
                                                                                                                               ttccTTATTAACGACTCGGTTATCTTCTCGGTGTAAAAAAATCGACTCTGCAAACGAACATCAAACGGGCGATCGTAAATATGCATAGACAGCAGGAGGATATGAATCGATCGCTTGCCTGAAAGCTTGTGATACGTTTg             <  1:461175/137‑1 (MQ=255)
                                                                                                                                ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc            >  1:1618997/1‑140 (MQ=255)
                                                                                                                                ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc            >  1:3166010/1‑140 (MQ=255)
                                                                                                                                ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCGGAAAGCTTGTCATACGTTTGc            >  2:2470037/1‑140 (MQ=255)
                                                                                                                                ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATAAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc            >  1:50228/1‑140 (MQ=255)
                                                                                                                                 tCTTATTAACGAATCGCTTATCTTCTGGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc           <  1:4128496/140‑1 (MQ=255)
                                                                                                                                 tCTTATGAACTAATCGCTTATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc           <  1:2760233/140‑1 (MQ=255)
                                                                                                                                 tCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc           <  2:1249610/140‑1 (MQ=255)
                                                                                                                                 tCTAATGAACGAATCGCTTAGCTTCCCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc           <  1:964440/140‑1 (MQ=255)
                                                                                                                                    tATTAACTAATCGGTTATGTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCAcc        <  1:1403651/140‑1 (MQ=255)
                                                                                                                                    tATGAACTAATCGCTGATCTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCAcc        <  2:5077375/140‑1 (MQ=255)
                                                                                                                                    tATGAACGAATCGCTTATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCAcc        <  1:4147893/140‑1 (MQ=255)
                                                                                                                                    tATGAACCAATCGCTTATCGTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCACACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCAcc        <  2:3079898/140‑1 (MQ=255)
                                                                                                                                     aTGAACGAATCGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc           <  2:1409777/136‑1 (MQ=255)
                                                                                                                                     aTGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc           >  1:1409777/1‑136 (MQ=255)
                                                                                                                                      ttaaCTAATCGCTTATTTTCTCGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta      <  2:3271415/138‑1 (MQ=255)
                                                                                                                                      ttaaCGAATCGCTGATCTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta      <  1:2248743/138‑1 (MQ=255)
                                                                                                                                      tGACCGAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta      <  2:2104446/140‑1 (MQ=255)
                                                                                                                                      tGAACGAATCGCTTATCTTCTCGTTGAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta      <  1:420945/140‑1 (MQ=255)
                                                                                                                                       gAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCtat     >  1:3657824/1‑140 (MQ=255)
                                                                                                                                       gAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCtat     >  1:1365306/1‑140 (MQ=255)
                                                                                                                                       gAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCtat     >  1:3719741/1‑140 (MQ=255)
                                                                                                                                        aaCTAAGCGCTGAGCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCtata    <  1:570013/140‑1 (MQ=255)
                                                                                                                                        aaCGAATCGCTGATCTTCTCGGTGTAAAAAAAACGACTGTGCAAACCACCATCAAAAGGGCGTACGTAAAGTTGCAAAAAAAGCAGGAGGAAATGAATAGATCCCTTTGCAGAAAGCTTTTCAAACGTTTTACAccaaaa    >  1:569167/1‑136 (MQ=255)
                                                                                                                                         aCGAATGGCTGATCTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa   <  2:2032267/140‑1 (MQ=255)
                                                                                                                                          cGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAg  >  2:225678/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAG  >  NZ_CP034943/1293189‑1293466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: