BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,720,712	C→A	13.6%	T212T (ACC→ACA)	spoIIIE →	DNA translocase SpoIIIE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,720,712	0	C	A	13.6%	366.2 / 13.5	168	T212T (ACC→ACA)	spoIIIE	DNA translocase SpoIIIE
Reads supporting (aligned to +/- strand): ref base C (81/58); new base A (3/26); total (84/84)
Fisher's exact test for biased strand distribution p-value = 2.43e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TTGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGA  >  NZ_CP034943/1720573‑1720851

tttttATGATTTTGATCGGAATTATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAGATCCGTCAAAAcc                                                                                                                                             <  2:4659122/137‑1 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                             >  2:2281762/1‑140 (MQ=255)

ttGTTATGATTTTGAGCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTTATGATATGAAATCCGTCAAAAcc                                                                                                                                             >  2:3763921/1‑140 (MQ=255)

                 ggAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGaa                                                                                                                            <  1:3151162/140‑1 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAgc                                                                                                                >  2:25290/1‑140 (MQ=255)

                              tCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAgcg                                                                                                               <  2:2080652/140‑1 (MQ=255)

                                  aGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGa                                                                                                           >  2:595651/1‑140 (MQ=255)

                                  aGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGa                                                                                                           >  2:3126728/1‑140 (MQ=255)

                                  aGGGCGCTCTCTGCAAGAAACGCTGAACAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGGTTGCATTTATTGATGCTATGAAATACGTCGAAACCCATATGAAGGCAGCGTACAAAAGGAAAGCGCGGa                                                                                                           >  2:3125042/1‑140 (MQ=255)

                                         tctctTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaaa                                                                                                    >  2:3609445/1‑140 (MQ=255)

                                         tctctTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaaa                                                                                                    >  2:3240528/1‑140 (MQ=255)

                                             ttCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATCAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaaaacaa                                                                                                >  1:4370601/1‑140 (MQ=255)

                                             ttCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAAACATATGAAGTCATCGTAAAAATCGACAGCGCCGAGcaaaaaacaa                                                                                                >  1:3073470/1‑140 (MQ=255)

                                              tCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAATCGCCGAGcaaaaaacaaa                                                                                               >  2:2051509/1‑140 (MQ=255)

                                                  gAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCaaaaaacaaaaaac                                                                                           >  2:977055/1‑140 (MQ=255)

                                                     aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                        >  1:603954/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACTAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:218618/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:3099222/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:4680308/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:4162005/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:1993671/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  1:3366559/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAAAAAAAAACGGccc                                                                                      >  2:388066/1‑140 (MQ=255)

                                                                 tGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACa                                                                            >  1:3442450/1‑140 (MQ=255)

                                                                  ggATGAGCCCTATTGGCCGCTTTATAAAAGAACAATTGTTTGCATTTATTGATGATATGAACTCCGTCAAAACAAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAg                                                                           <  2:1036292/140‑1 (MQ=255)

                                                                  ggATGAGCCCGATTGGCCGATTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAg                                                                           <  2:1208253/140‑1 (MQ=255)

                                                                    aTGAGCCCGTTTGGACGCTTTAAAAAATAACAATGGTTTGAATTTTTTGATGATATGAAATCCTTAAAAACAAATATGAAGTCACCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCa                                                                         <  2:4257351/140‑1 (MQ=255)

                                                                     tGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCaa                                                                        >  2:3540278/1‑140 (MQ=255)

                                                                      taGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCTTTTATTGATGATATTAAATCCTTCAAAACAAATATTAAGTCATCGAAAAAAACTAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCaaa                                                                       <  2:1346684/139‑1 (MQ=255)

                                                                      gAGCCCGATTGGCCGATTTATAAAAGAACAATGGTTTCCATTTATTGATGAAATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCaaa                                                                       <  2:1180898/140‑1 (MQ=255)

                                                                         cccGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAACTgg                                                                    >  1:528442/1‑140 (MQ=255)

                                                                              ttGGCCTATATATAAAATAAATTTGTTTTTCATTTTTTTATTATATTAAATCCGAAAAAACAAATATTAAGTAATCTAAAAAAACGAACGCGCCGAGAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCg                                                               <  1:2491621/140‑1 (MQ=255)

                                                                                ggCCGTTTTAAAAAACAAATTTGGTTTGATTTTATTTATTATATAAAACCCGAAAAACACAATATCAATCCAACTAAAACAACGAACGCGCCGATCAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                             <  1:339466/140‑1 (MQ=255)

                                                                                ggCCGCTTTATAAATGAACAATGGTTTGCATTTATTTATGATATGAAATCCGTCAAAACCAATATGAAGACATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                             <  2:2568124/140‑1 (MQ=255)

                                                                                ggCCGCTTTATAAAAGAACAATGGTTTGCATTTATTTATGATATCAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                             <  2:4757795/140‑1 (MQ=255)

                                                                                gccagCTTAATAAAACAACAATGGTTTGCATTTATTGATGCTATGAAATCCGTCAAAACAAATATGAAGTCATCGAAAAAAACGAAAGCGCCGCGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                             <  1:5033596/136‑1 (MQ=255)

                                                                                   ctcTTTATAAAATAACAATTGTTTGATTTTATTCATGATATCAAACCCTAAAAAACAAATATCACCTCAACCAAAAAAACCAACCCGCCCAGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:3943208/138‑1 (MQ=255)

                                                                                   cgttttATAAAATAACATTTTTTTGATTTTTTTGATGATATCAAAGCCCTCAAAACAAATTTGAAGTAAGCGAAAAAAACGAACCCGCCGAGAAAAAAAAAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:2471966/137‑1 (MQ=255)

                                                                                   cgatttATAAAATAACATTGTTTTGCATTTTTTGATGATATCAAAACCGAAAAAACAAATATGAAGTCATCGAAAAAAACGAACGCGCCGACCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:4944495/137‑1 (MQ=255)

                                                                                   cgatttATAAAATAACAATGTTTTGCATTTATTGATGATATGAAATCCGTCAAAACAAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  2:4557053/137‑1 (MQ=255)

                                                                                   cgatttATAAAAGAACAATGTTTTGCATTTATTGATGATATCAAACCCGACAAAACCAATATGACGACATCGAAAAAAACCAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:5107277/137‑1 (MQ=255)

                                                                                   cgatttAAAAAACAACATTTTTTTGCTTTTTTTGGTTTTATTAAAGCCGTAAAAACAAATATTATGTAATCAAAAAAAACTAACGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:4283620/137‑1 (MQ=255)

                                                                                   ccttttATAAAAGAACAATGGTTTGAATTTATTGATTATATGAAATCCGTAAAAACCAATATGAACTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  2:3276104/137‑1 (MQ=255)

                                                                                   cGCTTTATAAATTAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGACGTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  2:1557427/140‑1 (MQ=255)

                                                                                   cGCTTTATAAAAGAACACTGGTTTGCATTTATTGATGATATGACATCCGTCAAAACCAATAGGAAGTCATCGAAAAAAACGAACGCGCCGAGCAACAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  2:1730243/140‑1 (MQ=255)

                                                                                   cGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          >  2:1487075/1‑140 (MQ=255)

                                                                                   cGCTTTATAAAAGAAAATTGGTTTCAATTTATTGATTATATCAAACCCTACAAAACAAATATTAAGACAACTAAAAAAACGAAACCGCCGAGAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:4704552/140‑1 (MQ=255)

                                                                                     gtttATAAAAGAACAATGTTTTTCATTTATTGATGATATGAAATCCGTAAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                        <  2:3834262/139‑1 (MQ=255)

                                                                                     cTTTATAAAACAACAATGTTTTGCATTTATTGATGATATTAAATCCTTAAAAACCAATATGAAGTCATCGAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                        <  2:4330882/140‑1 (MQ=255)

                                                                                        tataAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATTTGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                     <  2:3741480/140‑1 (MQ=255)

                                                                                        tataAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                     >  1:1732467/1‑140 (MQ=255)

                                                                                        tataAAACAACACTGGTTTGAATTTATTGATGATAGCAAATCCGAAAAAACAAATATGAAGCAACCGAAAAAAACCAACGCCCCGACAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                     <  1:4496762/140‑1 (MQ=255)

                                                                                        tataAAACAACAATGGTTTGCATTTATTGATGATATTAAATCCGTCAAAACCAATATGAAGCCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                     <  2:4376297/140‑1 (MQ=255)

                                                                                                aaCAATGGTTTGCATTTATTGATGATATGAAATCCGTCCAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACTAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCGTTCTGCTGAagag                                             >  2:4921157/1‑140 (MQ=255)

                                                                                                aaCAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAagag                                             >  2:3698128/1‑140 (MQ=255)

                                                                                                aaCAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATAGAAAAAAACTAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGAAAATGGAACCGGAGCCTTCTGCTGAagag                                             >  1:1488911/1‑140 (MQ=255)

                                                                                                    aTGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAggggg                                         >  2:3349528/1‑140 (MQ=255)

                                                                                                        tttGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACACAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCGGCTGAAGAGGGGGAttt                                     >  2:938488/1‑140 (MQ=255)

                                                                                                        tttGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGAttt                                     >  2:271766/1‑140 (MQ=255)

                                                                                                          tGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGACGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCGGACGAGGGGGATTTgg                                   >  1:5129664/1‑140 (MQ=255)

                                                                                                          tGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                   >  1:2819744/1‑140 (MQ=255)

                                                                                                          tGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                   >  1:1601793/1‑140 (MQ=255)

                                                                                                          tGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                   >  1:1608482/1‑140 (MQ=255)

                                                                                                          tGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                   >  1:3433917/1‑140 (MQ=255)

                                                                                                             tttgaTTTATGATATCAAATCCGACAAACCCAATATTAAGTAATCGAAAAAAACGAAAGCGCCTAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaaa                                <  1:1086659/136‑1 (MQ=255)

                                                                                                             aTTTATTGATGATATCAAATCCGTCAAAACCAATATGAAGACATCTAAAAAAACCAAAGCGCCGAGAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaaa                                <  1:1082692/140‑1 (MQ=255)

                                                                                                                        atctcaaaTCCGAAAAAACAAATATGAAGTCATCGAAAAAAACTAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  1:4666615/135‑1 (MQ=255)

                                                                                                                        atatGAATTCCGTCAAAACCAATATGAAGGCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:3424157/140‑1 (MQ=255)

                                                                                                                        atatGAAATCCTTAAAAACAAATATGAAGTCAGCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:1724647/140‑1 (MQ=255)

                                                                                                                        atatGAAATCCGTCAAAACCAATATTACGTCAACGAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:1452928/140‑1 (MQ=255)

                                                                                                                        atatGAAATCCGTCAAAACCAATATGAAGTCCTCGAAAAAAACGAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:2149389/140‑1 (MQ=255)

                                                                                                                        atatGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:4413615/140‑1 (MQ=255)

                                                                                                                        atatGAAATCCGACAAAACCAATATGAAGTCATCGAAAAAAACGACCGCGCCGAGCAAAAACCAAAACACGGCCCGCACAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:3479688/140‑1 (MQ=255)

                                                                                                                        atatCAAATCCGTAAAAACCAATATTAAGTCATCGAAAAAAACCAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  1:4112252/140‑1 (MQ=255)

                                                                                                                        atatCAAAGCCGACAAACAAAATATGAACAAATCCAAAAAAACCAACGCGCCGACAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  1:465660/140‑1 (MQ=255)

                                                                                                                        atatCAAACCCGACAAAACCAATATGAACTCATCGAAAAAAACGAACGCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  1:3857761/140‑1 (MQ=255)

                                                                                                                        ataaGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCCAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:1110465/140‑1 (MQ=255)

                                                                                                                        agatcaaaTCCGTCAAAACCAATATGAAGACATCGAAAAAAACGACCGCGCCTAGCAAAAAAAACAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  1:3619770/135‑1 (MQ=255)

                                                                                                                         tatGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctc                    >  2:2242026/1‑140 (MQ=255)

                                                                                                                             aaaTCCGTCAAAACCAATATGAAGTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  2:515062/140‑1 (MQ=255)

                                                                                                                             aaaTCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  2:533249/140‑1 (MQ=255)

                                                                                                                             aaaTCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  2:603954/140‑1 (MQ=255)

                                                                                                                             aaaTCCGTCAAAACCAATATGAAGTCACCGAAAAAAACGAACGCGCCGAGCAAAAAACAAACAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  2:2555147/140‑1 (MQ=255)

                                                                                                                             aaaTCCGTCAAAACAAATATGAAGTCATCGAAAAAAACGAACGCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  1:3550888/140‑1 (MQ=255)

                                                                                                                             aaaTCCCTAAAAACCAATATCACTTCATCTAAAAAAACGAAAGCGCCGACCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  1:3208953/140‑1 (MQ=255)

                                                                                                                               aTCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattc              >  2:4833465/1‑140 (MQ=255)

                                                                                                                                 ccGTCATAACAAATATGAAGTAATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCATCTTattcat            >  2:2480637/1‑140 (MQ=255)

                                                                                                                                 ccGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattcat            >  2:2096508/1‑140 (MQ=255)

                                                                                                                                   gTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattcattc          >  1:2758090/1‑140 (MQ=255)

                                                                                                                                   gTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattcattc          >  1:2757342/1‑140 (MQ=255)

                                                                                                                                   gTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGAGTTGGAAACGGTATCGCCTCTTattcattc          >  1:4431118/1‑140 (MQ=255)

                                                                                                                                    tCATAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACTGCAAATGGATCCGGAGCCTTCTGATGGAGAGGGGGATTTGGAAACGGGATCGCCTCGTattcattaa         >  1:2287481/1‑138 (MQ=255)

                                                                                                                                    tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACCGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGAttt                                     <  2:4079678/112‑1 (MQ=255)

                                                                                                                                    tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca         <  2:497048/140‑1 (MQ=255)

                                                                                                                                    tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca         >  2:2213350/1‑140 (MQ=255)

                                                                                                                                    tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACAGTATCGCCTCTTAttcattca         >  2:850416/1‑140 (MQ=255)

                                                                                                                                        aaCCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGc     >  2:2825418/1‑140 (MQ=255)

                                                                                                                                           cAATATGAAGTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGa  <  2:1732467/140‑1 (MQ=255)

                                                                                                                                           cAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGa  <  2:5094689/140‑1 (MQ=255)

                                                                                                                                           cAATATGAAGTCAGCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAACTGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGa  <  2:170849/140‑1 (MQ=255)

TTGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGA  >  NZ_CP034943/1720573‑1720851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`