BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,190	G→T	17.1%	F114L (TTC→TTA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,190	0	G	T	17.1%	848.5 / 54.4	475	F114L (TTC→TTA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (210/167); new base T (94/4); total (304/171)
Fisher's exact test for biased strand distribution p-value = 2.95e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 508 total aligned reads displayed.

CGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGA  >  NZ_CP034943/240051‑240328

cGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTtttgtttg                                                                                                                                            >  2:4736035/1‑140 (MQ=255)

 ggCTGTGGTGGAGAAGGTGTACGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGa                                                                                                                                           <  2:5009436/140‑1 (MQ=255)

 ggCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGa                                                                                                                                           <  2:1203009/140‑1 (MQ=255)

   cTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTAg                                                                                                                                         >  2:3632581/1‑140 (MQ=255)

   cTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAAg                                                                                                                                         >  1:3257564/1‑140 (MQ=255)

   cTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGaat                                                                                                                                         >  1:515616/1‑139 (MQ=255)

      tggtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAtttt                                                                                                                                      >  2:1998158/1‑140 (MQ=255)

      tcgtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTAATTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGAGTTTTAGGATTTTTGTTTGAAtttt                                                                                                                                      >  2:2222210/3‑140 (MQ=255)

           gagaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGga                                                                                                                                 >  1:436449/1‑140 (MQ=255)

                ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGAttt                                                                                                                            >  1:3210914/1‑140 (MQ=255)

                ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTCTGTTTGAAGTTTTGGGAGAttt                                                                                                                            >  2:1758980/1‑140 (MQ=255)

                  tgtAAGAGAGAGAGAAGGAGCATCACTGATGTTGGAATTATTCAGAGATGATGTTTATGTATTTTTGGTCTAATACGAAGTCGAACAATCCGACAATACCGCATTTTTCGGAATTTTGTTTGAAGATTTTGGAGATTAGa                                                                                                                          >  2:2545697/1‑140 (MQ=255)

                   gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTGGGAGATTTGaa                                                                                                                         >  2:2603103/1‑140 (MQ=255)

                     aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGATATTTGAATg                                                                                                                       >  2:4160345/1‑140 (MQ=255)

                     aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATg                                                                                                                       >  2:632911/1‑140 (MQ=255)

                     aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATg                                                                                                                       >  2:524712/1‑140 (MQ=255)

                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGGTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGAGTATACGGGCGTTTTGGGATTTTTGTTTTAATTTTTTTGTGATTTGAATGGCttt                                                                                                                  >  2:3405996/1‑140 (MQ=255)

                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTTTATgggtgt                                                                                                                  >  2:3267657/1‑136 (MQ=255)

                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTTAATTGCttt                                                                                                                  >  1:2810718/1‑140 (MQ=255)

                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCttt                                                                                                                  >  2:714619/1‑140 (MQ=255)

                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTAGGATTTTTGTTTGAATTTTTGTGAGATTTGAATGGCttt                                                                                                                  >  2:4117848/1‑140 (MQ=255)

                           gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTTGGAGATTTTAATGGCtttt                                                                                                                 >  1:2018562/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTTATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTTTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGAATTTTGTTTGAAGTTTAGTGAGATATGAATGGCTTTTc                                                                                                                >  1:1181603/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGTATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCgttta                                                                                                                >  1:4883255/1‑139 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                >  1:5034056/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATGGCgtttg                                                                                                                >  1:3098156/1‑139 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATGGGTTTTc                                                                                                                >  2:4915069/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCtttta                                                                                                                >  2:2689083/1‑139 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                >  1:2312503/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                >  2:2369795/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTGTGATATTTGAATGGCtttcc                                                                                                                >  2:1006632/1‑138 (MQ=255)

                             gagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTcc                                                                                                               <  1:2840686/140‑1 (MQ=255)

                                  ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTGTAGTTTTTGGAGATTTTAATTGCTTTTGCGtttt                                                                                                          >  1:1532657/1‑140 (MQ=255)

                                  ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGtttt                                                                                                          >  2:84359/1‑140 (MQ=255)

                                    aGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTa                                                                                                        >  2:2035657/1‑140 (MQ=255)

                                    aGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTGAAGGTTTGGTAGATTTTTATGGCTTTTCCGTTTTTa                                                                                                        >  2:1841488/1‑140 (MQ=255)

                                      cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGATTTTCCGTTTTTaaa                                                                                                      >  2:4369930/1‑140 (MQ=255)

                                        tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTTGAGTTTTGGGTGATTTTGATTGCTTTTCCGTTTTTaaaaa                                                                                                    >  1:2149933/1‑139 (MQ=255)

                                        tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTGGGAGATTTTAATTGCTTTTACGTTTTTaaaaa                                                                                                    >  1:3484779/1‑139 (MQ=255)

                                        tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                    >  1:1057839/1‑140 (MQ=255)

                                              aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                              <  2:4519568/140‑1 (MQ=255)

                                                  tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                          <  1:4920591/140‑1 (MQ=255)

                                                  tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGGTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                          <  1:4062245/140‑1 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACTAAGTCGATCATTACGACTATACGGGCGTGTTCGGAATTTTTTTTGTACTTTTTGGTGTTTTTAAATTGTATTAACTTTTTAAAAAAAGATGCaaac                                                                                         >  1:2266728/1‑138 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCGTTTCAGTTTTTAAAACAATATGCCAGc                                                                                         >  2:2795863/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                         <  1:950921/140‑1 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTGTTCCGTTTTTAAAAAAAGATGCAAGc                                                                                         >  2:3871238/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCGGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTAGTTTTGGGAGATTTGAATGTCTTTTCCGTTTTTAAAACACTAGGCAAGc                                                                                         >  2:3580641/1‑140 (MQ=255)

                                                    gAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGcc                                                                                        >  2:1659543/1‑140 (MQ=255)

                                                      attattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAg                                                                                      >  2:913718/1‑140 (MQ=255)

                                                        tattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGAt                                                                                    <  2:3749979/140‑1 (MQ=255)

                                                         attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATa                                                                                   >  2:2871618/1‑140 (MQ=255)

                                                         attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                   >  2:3933121/1‑140 (MQ=255)

                                                         attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                   <  1:1746779/140‑1 (MQ=255)

                                                               agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAACAGGCAACCCACATAAACCCg                                                                             >  2:4660445/1‑140 (MQ=255)

                                                               agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTTAGTTTTTGGAGATTTGAATTGCTTTTACGTTTTTAAAAAAAGAGGCAATCCACATAAAaccc                                                                             >  1:1294586/1‑139 (MQ=255)

                                                               agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCg                                                                             >  1:3614448/1‑140 (MQ=255)

                                                               agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCg                                                                             >  2:3162305/1‑140 (MQ=255)

                                                               agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAATTTTTTGGAGATTTTTATTGCTTTTCCGTTTTTAAAACAAGAGGCAACCCACAAAAAcccc                                                                             >  1:795981/1‑139 (MQ=255)

                                                                  tGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGaca                                                                          <  1:579154/140‑1 (MQ=255)

                                                                    cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTGCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGacaac                                                                        >  2:1389807/1‑140 (MQ=255)

                                                                    cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGGTTTTCCGTTTTTTAAACAAGAGGCAAGCCAGATAAAACCGacaac                                                                        >  2:2150095/1‑140 (MQ=255)

                                                                    cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGATGCAATCCAGATAAACCCGacaac                                                                        >  2:5047143/1‑140 (MQ=255)

                                                                    cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTGGGTGATTTGAATGGCTTTTCCGTTTTTTATAAAATAGGCAAGCCCGATAAACACGacaac                                                                        >  1:2487600/1‑140 (MQ=255)

                                                                         tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTTAGTTTTTGGGGATTTTAATTGCGTTTGCGTTTTTAAAAAAATAGGGAACCCAGATAAACCCCAaaacaacaa                                                                   >  1:1352302/1‑140 (MQ=255)

                                                                                 tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTATTTTTTGGAGGTTTGAATGGCTTTTTCGTTTTTTAAAAAAGAGGCAAGCCCCATAAACCCGACAACAAAAAAAaataat                                                           >  1:4589115/1‑140 (MQ=255)

                                                                                 tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTGTAGTTTTTGGGGTTTTGTATTGGTTTTGCGTTTTTTAAACAACATGCAAGCCACATAAAACCGCACACAACAaaaaaaaaa                                                           >  1:1865730/1‑139 (MQ=255)

                                                                                 tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGCTTTTTGTTTTAAGTTTTTGGAGATTTTAATTGCTTTTACGTTTTTTAAAAAAGAGGCAAGCCAGATAAACACCACAACaaaaaaaaaaaaa                                                           >  1:1929072/1‑139 (MQ=255)

                                                                                 tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGGTCGGGTTTTTTTTTGTAGTTTTGGGTGATTTGTATGGCTTTTGCGTTTTTAAAAAAAGTGGCAAGCCAGATAAAACCGCCAACAACAAAAaataaa                                                           >  1:1466007/1‑139 (MQ=255)

                                                                                   tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTGCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                         <  2:3112002/140‑1 (MQ=255)

                                                                                     ttCTAATCCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                       <  1:3010978/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                       <  1:3831333/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                       <  1:1659543/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                       <  2:180694/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                       <  1:4474615/140‑1 (MQ=255)

                                                                                          aTACGAAGTCGATCATTCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                  <  2:5117329/140‑1 (MQ=255)

                                                                                           tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                 <  1:4185101/140‑1 (MQ=255)

                                                                                           tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                 <  2:1306915/140‑1 (MQ=255)

                                                                                           tACGAAGTCGATCATTCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                 <  2:4431618/140‑1 (MQ=255)

                                                                                           gaCGAACTCGATCATTCCGACTATACTGGCGTTTTCTGATTTTTGTTTTAAGTTTTGGGAGATTTCAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCTACAACAACAAAAAATAATCTAGCAAGTa                                                 <  2:2924666/139‑1 (MQ=255)

                                                                                              gAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAAc                                              <  2:3210914/140‑1 (MQ=255)

                                                                                               aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACt                                             >  2:425654/1‑140 (MQ=255)

                                                                                                aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                            <  2:2087068/140‑1 (MQ=255)

                                                                                                 gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGTGAGATTTTAATGGATTTTCATTTTTTAAAACAAGAGGCAAGCCAGATAAACCCTTCAAAAAAAAAAAATAATCTAGCAAGTatagtct                                           <  2:2405793/140‑7 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCACGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  2:114240/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  1:1140024/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  1:3952725/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  1:2899/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  1:4828942/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  1:709960/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  1:922734/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                      <  2:2115709/140‑1 (MQ=255)

                                                                                                                   aCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCACATAAAACCGACAAAAAAAAAAAATAATATAGCAAGTAAACTCCATGCATGGGGTAattcat                         >  2:14679/1‑140 (MQ=255)

                                                                                                                        cGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACGAGAGGCAAGCCAGATTAACCCGACCACAACAAAAAATAATATAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGtt                    >  2:1545825/1‑140 (MQ=255)

                                                                                                                          ttttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCAGTCGTTGc                  <  2:4836344/140‑1 (MQ=255)

                                                                                                                           tttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCa                 <  1:1589601/140‑1 (MQ=255)

                                                                                                                                      tgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa      <  1:2664719/140‑1 (MQ=255)

                                                                                                                                      tgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa      <  2:4136140/140‑1 (MQ=255)

                                                                                                                                        tttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATa    <  1:3386429/140‑1 (MQ=255)

                                                                                                                                         ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag   <  1:5084876/140‑1 (MQ=255)

CGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGA  >  NZ_CP034943/240051‑240328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`