Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,730,080 A→C 6.1% K167N (AAA→AAC rodZ → cell shape determination protein RodZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0800AC 6.1% 257.4 / 4.2 116K167N (AAA→AACrodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base A (57/48);  new base C (3/6);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 123 total aligned reads displayed.

GGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGA  >  NZ_CP034943/1729943‑1730216
                                                                                                                                         |                                                                                                                                        
ggTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGaaagaaaaaga                                                                                                                                        >  2:2485975/1‑140 (MQ=255)
ggTAACAATGCCGCATCTGAAAAATCAATTACGAAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGaaagacaaaga                                                                                                                                        >  2:2594919/1‑140 (MQ=255)
    aCAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGACAGAAAAAGAAGAt                                                                                                                                    >  1:1819025/1‑140 (MQ=255)
      aaTGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTCAAAAAGAAACGAAAGAAAAAGAAGATaa                                                                                                                                  >  2:4825381/1‑140 (MQ=255)
      aaTGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaa                                                                                                                                  >  2:3754978/1‑140 (MQ=255)
        tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                                >  1:3218736/1‑140 (MQ=255)
        tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                                >  2:142323/1‑140 (MQ=255)
        tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                                >  2:3982750/1‑140 (MQ=255)
        tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTAAACGCTAAAAGAGACTCAAAGCAACAGCTCTGAAAAAGCGGCAGACACTAAAAAAGAACCGATAGACATCGAAGATaaac                                                                                                                                >  1:492523/1‑139 (MQ=255)
          ccGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaaaa                                                                                                                              >  2:4570020/1‑140 (MQ=255)
             cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACTCTAAAAGAGAATCAACGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAACAGGAAGATAAAAaagaa                                                                                                                           >  2:2092987/1‑140 (MQ=255)
             cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTCAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                           >  2:639492/1‑140 (MQ=255)
             cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                           >  2:5010407/1‑140 (MQ=255)
             cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                           >  1:83256/1‑140 (MQ=255)
             cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                           >  2:2751779/1‑140 (MQ=255)
                 tGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAACAAGAAACGAAAGAAAAAGAAGATAAAAAagaagact                                                                                                                       <  1:2462223/140‑3 (MQ=255)
                 tGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAagaagact                                                                                                                       <  1:3420856/140‑3 (MQ=255)
                     aaataaatTACGAAAATTGAAAGAAATTACTAAATCCAAAAATATTCAACGCTAAAAGATAATCAAAGCAACAGGTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAACGAAGAAAGCGa                                                                                                                   <  2:348708/140‑1 (MQ=255)
                                  aatgttaaaGCAAGTACGAAATACCAAAAGATTCAACGATAAAACATAATAAAATCAACCGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACGAAACAAAAATAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                      <  2:1259162/134‑1 (MQ=255)
                                    aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAACAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTCAAAAAGAAACGAACGAACAAGAAGATACAAAAGAAGACAGCGACAGTGATCATCTGga                                                                                                    >  1:1309419/1‑140 (MQ=255)
                                    aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGAGAAAAAAGAAGAAAGCGACAGTGATAAACTGga                                                                                                    >  1:2536666/1‑140 (MQ=255)
                                              aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGCAGAAAGCGACAGTGATAAACGGGAGATTAAAGCa                                                                                          >  2:1372656/1‑140 (MQ=255)
                                              aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                          >  2:338968/1‑140 (MQ=255)
                                              aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                          >  2:2201622/1‑140 (MQ=255)
                                              aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGAATAAAgaa                                                                                          >  2:3623906/1‑138 (MQ=255)
                                              aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGAATAAAGCa                                                                                          >  2:3623907/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGTGGCCGACACTAAAGATGAAACGAAAGAAAAAGAAGAGACAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:1486865/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGAGAGTGAGAAACTGGAGATTAAAGAAAc                                                                                        >  1:2817524/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:1106554/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:485013/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:4430460/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:1806066/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:2308948/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:2817762/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:2963672/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:3715428/1‑140 (MQ=255)
                                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        >  1:3667023/1‑140 (MQ=255)
                                                tACCAAAACCCAAAACATCAAACCCAAAACCAGAATCAACCCAACACCTCTGAAAAAGAGGAACACACAAAAAAACAACCCAAACAAAAAGAACATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        <  1:512398/140‑1 (MQ=255)
                                                tACCAAAACCAAAAAGATACAACGAAAAAACAAAAAAAAAGCACCAGCTATTAAAATGAGCCACACAAAAAAAAACAACCCAACCAAAAAGAACATAAAAAAGCACAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                        <  1:5178749/140‑1 (MQ=255)
                                                aaccaaaCCCAAAAATATAAAACCCTAAAACAAAATAAAACCAACAGCAATTAAAACAAGCCAGACAATAAAAAACAAACCAAATAAAAAGACGAAAAAACCGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCacc                                                                                        <  1:4928519/136‑3 (MQ=255)
                                                  cGAAATCCAAAAAGTTTCAACGATAAAAGAGAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACAATAAAAAAGAAACGAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                      <  2:4499916/140‑1 (MQ=255)
                                                       tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGAGACAGTGATAAACTGCAGATAAAAGCAACTGGCACt                                                                                 >  2:4592714/1‑140 (MQ=255)
                                                         caaaaaGATAAAACCATAAAAGACAATAAAACAAACAGATCTTAAAAAGAGCCACAAAATAAAAAACAAACGAAACAAAAATAAGAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                               <  1:4964759/138‑1 (MQ=255)
                                                          cAAAAGATTCACCGCTAACAGACAATCAAACAAACAGCTCTTAAATAGAGGCACACAATAAAAAAGAAACTAAATAAAAACAAGATAACAAACTCGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                              <  1:807949/140‑1 (MQ=255)
                                                          cAAAAGATACAACGCTAAAATAGAAACAAACCAACAGCTCTCAAAAAGAGGCAGACACTAAAAACTAAACGAAACAAAACGAAGATAAAAACGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                              <  1:3609930/140‑1 (MQ=255)
                                                          aaaaattttCAACGATAAAACACAATAAAACCAACAGCTCTTAAAAAGAGGCACACAATAAAAAACAAACCAAACAAAAAGAACATAAAAACCAACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                              <  1:564616/133‑1 (MQ=255)
                                                             aaGATACAACGCTAAAAGATAAAAAAAGAAACAGCTCTGAAAAAGAGCCACACACTAAAAAACAAACTAAAGAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                           <  2:206654/140‑1 (MQ=255)
                                                             aaGATACAACGATAAAAGACAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                           <  2:18758/140‑1 (MQ=255)
                                                                     ccccTAAACGACAATAAAACCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAACCGAAACAAAAACACGATAAAAACGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  1:1301487/137‑1 (MQ=255)
                                                                     acccaaaaaGACAAAAAAACAAACCGATCTTAAAAAGAGCCACACAATAAAAAACAAACCAAACAAAAACAAAATAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  1:655419/135‑1 (MQ=255)
                                                                     aCGCTAAAAGAGAATAAAAGCAACAGCTATCAAAAAGAGGCCCACAATAAAAATGAAACGAAATAAAACTAAGATAAAAACGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  2:3346686/140‑1 (MQ=255)
                                                                     aCGCTAAAAGAGAATAAAAGCAAAAGCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACTAAAGAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  2:2401958/140‑1 (MQ=255)
                                                                     aCGCTAAAACACAAACAAACCAACATCACATAAAAAGAGGCATACACTAAAAAACAAACGAAACAAAAAGAACATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  2:3028569/140‑1 (MQ=255)
                                                                     aCGATAAAATACAAAAAAAGCAACACCTCTCAAAATGAGGCATACACTAAAAAATAAACGAAAGAAAAACAACCTAAAAAACACGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  1:2904151/140‑1 (MQ=255)
                                                                     aCGATAAAAGAGAATCAAAGCAAAAGATCTGAAAAAGAGGAAGACACTAAAAAAGAAACTAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  2:5144465/140‑1 (MQ=255)
                                                                     aCGATAAAACATAATAAAACCAACCCCTCTGAAAAAGAGCAACACACTAAAAAAGAAACCAAACAAAAACAATAAAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  2:4609878/140‑1 (MQ=255)
                                                                     aCGATAAAACAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                   <  2:2203309/140‑1 (MQ=255)
                                                                          aaaaGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAACAAAGAAACCAAAGAAAAAGAAGATAAAACCGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCACCTGGCACTGAAGGGTCATTAACGACtt                                                              <  2:1145089/140‑1 (MQ=255)
                                                                               agaATCAAACCAACAGATCTTAAAAATAGCCAGACACTAAAAAAGAACCGAACGAAACTCACCATACAAAACACGAACGCGACAGTTCTAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                         <  2:4717674/140‑1 (MQ=255)
                                                                                 aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTCACGACTTATGAAgt                                                       >  1:3022182/1‑140 (MQ=255)
                                                                                 aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                       >  2:2801761/1‑140 (MQ=255)
                                                                                 aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                       >  2:1717659/1‑140 (MQ=255)
                                                                                 aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                       >  1:4657009/1‑140 (MQ=255)
                                                                                   tCAAAGCAACAGCTCTGAAAAAGAGCAAGACACAAAAAAAGAAACGAAATAAAAAGAAGATAAAAAAGAAGAAAGCGACACTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGCTCAATAACGACTTATGAActgt                                                     >  2:5120118/1‑140 (MQ=255)
                                                                                    cAAACCAACAGCTCTCAAAAAGACCCACACAAAAAAAAACAAACCAAACAAAAACAAGAAAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                    <  1:187729/140‑1 (MQ=255)
                                                                                         caccAGCTCTGAAAAACAGGCACACACTAAAAAAGAAACCAACGAAAACCAAGATAAAAAACACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGgcg                                               <  2:1819025/137‑1 (MQ=255)
                                                                                                 ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGaaa                                       >  1:2063094/1‑140 (MQ=255)
                                                                                                 ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGaaa                                       >  1:2135360/1‑140 (MQ=255)
                                                                                                         gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                               >  2:4604200/1‑140 (MQ=255)
                                                                                                         gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                               >  2:1257183/1‑140 (MQ=255)
                                                                                                         gagGCAGACACTAAAAAAGAAAAGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGTAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                               >  2:1269590/1‑140 (MQ=255)
                                                                                                             cAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCTGCGCTGAGAAATTCGAGCTCGaa                           >  1:3896559/1‑140 (MQ=255)
                                                                                                                acacTAAAAAAGAAACGAAAGAAAACGAAGCTAAGAAAAAAGAAAATGACAGTGAGAAGCTGGAGATTAAAGCTGCTGGCACTGAAGGATCGTTAACGACTTATGAAGTGTCCGGCGCTGATAAAATCGAGCTGGAACTg                        >  1:1184762/1‑140 (MQ=255)
                                                                                                                acacTAAAAAAGAAACGAAAGAAAACGAAGATAAGAAAAAAGAAAATGACAGTGAGAAGCTGGAGATTAAAGCTGCTGGCACTGAAGGATCGTTAACGACTTATGAAGTGTCCGGCGCTGATAAAATCGAGCTGGAACTg                        >  1:2499874/1‑140 (MQ=255)
                                                                                                                 cacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                       <  2:3667023/140‑1 (MQ=255)
                                                                                                                 cacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                       >  1:782951/1‑140 (MQ=255)
                                                                                                                 cacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                       >  1:657136/1‑140 (MQ=255)
                                                                                                                 aacTAAAAAAGAAACCAAACAAAAACAACATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                       <  2:3389159/139‑1 (MQ=255)
                                                                                                                 aaatAAAAAATAAACGAAACAAAAAGACGATAAAAATGAATAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGAATTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                       <  2:1773615/137‑1 (MQ=255)
                                                                                                                      aaaaaGAAACGAAATAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAATCAACACGCACTGAACGGTCATTAACGACTTATTAAGTGACCGGACCTGAGAAATTCGAGCTAGAACTCAACGCa                  >  2:4109054/1‑140 (MQ=255)
                                                                                                                      aaaaaGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATAAAAGCAACTGGCACTCAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCTATCACGAACTGaatgaa                  >  2:2733512/1‑136 (MQ=255)
                                                                                                                           taaaCTAAATAAAAACAACATAAAAAATAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  2:2047817/139‑1 (MQ=255)
                                                                                                                           taaaCGAACTAAAAAGACTATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  2:782951/139‑1 (MQ=255)
                                                                                                                           taaaCGAAACAAAAATAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  2:412963/139‑1 (MQ=255)
                                                                                                                           gAAACTAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  2:447294/140‑1 (MQ=255)
                                                                                                                           gAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGGCAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  2:780795/140‑1 (MQ=255)
                                                                                                                           gAAACCAAACAAAAAGAACATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  1:4195273/140‑1 (MQ=255)
                                                                                                                           caaaCGAAACAAAAACAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  1:4744472/139‑1 (MQ=255)
                                                                                                                           caaaCCAAAGAAAAACAAGATAAAAAAGACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  1:717194/139‑1 (MQ=255)
                                                                                                                           caaaCCAAACAAAAAGAACAAAAAAAAGACCAACCCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  1:3973506/139‑1 (MQ=255)
                                                                                                                           caaaCCAAACAAAAACAAGCTAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  1:2201622/139‑1 (MQ=255)
                                                                                                                           caaaCCAAACAAAAACAAGATAAAAAAGAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa             <  1:4713042/139‑1 (MQ=255)
                                                                                                                            aaaCGAACGACAACGAAGATAAGAAAAAAGAAAATGACAGTGAGAAGCTGGAGATTAAAGCTGCTGGCACTGAAGGATCGTTAACGACTTATGAAGTGTCCGGCGCTGATAAAATCGAGCTGGAACTGAAGGCATCAGAc            <  2:1184762/140‑1 (MQ=255)
                                                                                                                            aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc            >  2:733557/1‑140 (MQ=255)
                                                                                                                            aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAATGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc            >  2:4884654/1‑140 (MQ=255)
                                                                                                                              aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg          >  1:793918/1‑140 (MQ=255)
                                                                                                                                 acccaacaagaacCTAAAAAAGAACAACTCGACACTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTc       <  1:4335147/133‑1 (MQ=255)
                                                                                                                                  aagaaaaagaaGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCt      >  2:4481623/1‑140 (MQ=255)
                                                                                                                                  aagaaaaagaaGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCt      >  2:3890296/1‑140 (MQ=255)
                                                                                                                                      aaaagaaCATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa  <  1:1009911/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGA  >  NZ_CP034943/1729943‑1730216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: