Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,800,874 A→C 16.4% S3519R (AGC→CGC)  EO946_RS09135 → SDR family NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,800,8740AC16.4% 517.0 / 28.6 250S3519R (AGC→CGC) EO946_RS09135SDR family NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (132/67);  new base C (2/49);  total (134/116)
Fisher's exact test for biased strand distribution p-value = 2.63e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

ATTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAACGC  >  NZ_CP034943/1800738‑1801011
                                                                                                                                        |                                                                                                                                         
aTTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGCAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCg                                                                                                                                        >  2:3162580/1‑140 (MQ=255)
   gATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGAcc                                                                                                                                     >  1:4741899/1‑140 (MQ=255)
   gATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGATCTCGTACCAGAAAAAAATAATAAAAgagaac                                                                                                                                     >  1:3510359/1‑138 (MQ=255)
    aTCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCg                                                                                                                                    >  2:2228867/1‑140 (MQ=255)
     tgtgCTTATTTTTAACGGATCACAAAGAGCTGTTCTAAGCATTTTACGGGGATTATGCGTGTTAGGAACAAAAGCCCCCTGAAGCTGATATGTAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCgg                                                                                                                                   <  2:460870/137‑1 (MQ=255)
     tCGGCTTATTCTTAACGGATAACAAAGAGCTTATCTAAGCATTTTACCGGGTTTATGCGTCTGAGGCACAAACGCCCCCTGAAGCTTATATGTAATTTCCTGCAGATCTCGTACCAGAAACAAATAATATAAGCGACCgg                                                                                                                                   <  2:1567196/140‑1 (MQ=255)
      cGGTTTATTCTTAACGCAACACAAAGAGCTGATCTAAGATTTTGACCGGGATTATGGGTCTGAGGCACAAAAGCCCCCTGAAGCTGATTTGTAAATTCCTGCAGCTCTCGTACCACAAAAAAATAATAAACGCGACCGGc                                                                                                                                  <  1:1014261/140‑1 (MQ=255)
       ggCTTCTTCTTAACGGATCACAAAGACCTCATCTAAGCATTTTACCGGTATGATGCGTCTGAGGCAAAAAAGCCCCCTTAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCa                                                                                                                                 <  2:4376303/140‑1 (MQ=255)
         cttcttcttAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATCAGCGACCGGCAAt                                                                                                                               >  1:201442/1‑140 (MQ=255)
                  aaCGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCg                                                                                                                      >  2:3731571/1‑140 (MQ=255)
                  aaCGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCg                                                                                                                      >  2:4889239/1‑140 (MQ=255)
                   aCGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGa                                                                                                                     >  2:3694624/1‑140 (MQ=255)
                   aCGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGa                                                                                                                     >  2:5034432/1‑140 (MQ=255)
                   aCGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAAAATAATCGACCGGCAATCTCACGCCGa                                                                                                                     >  1:2277798/1‑140 (MQ=255)
                      gATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGcc                                                                                                                  >  1:1989158/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  2:4833258/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  1:1028228/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  1:4264608/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  2:591142/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  1:809342/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  2:1386928/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  2:3151383/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  2:377580/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  2:3826982/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                 >  1:3085325/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACAGGGATGATGCGTCTGAGTCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGAAGCTCTCGTACCAGAAAACAATAAAATAAGCGACCGCCAATCTCACGACGAGCCt                                                                                                                 >  2:1256546/1‑140 (MQ=255)
                         cacaAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTAt                                                                                                               >  1:3730204/1‑140 (MQ=255)
                         cacaAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCGAt                                                                                                               >  1:658473/1‑140 (MQ=255)
                            aacgcgCTGACCGACGATTTTTCCGGGGTTGATGCGTCTGCGGCAAAAAAGCCCCCTGAAGCTGATATGTAATTTCGTGCAGCTGTCGTACCCGAAAACAATAATACACGCGACCGGCAATCTCACGCCGAGCCTATCGc                                                                                                            <  1:4556618/135‑1 (MQ=255)
                            aaaGTGCTTATCTAAGCTTTTTACCGGGATTATGGGTCTGAGCCAAAAACGCCCCCTGAAGCTGATATGCAAATGCCTGCAGCTCTCGTACCACAAAACAATAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGc                                                                                                            <  1:29878/140‑1 (MQ=255)
                            aaaGCGCTGATCTAACCATTTGACCGGGAGGATGCGTCTGAGGCACAAACCCCCCCTGAACCTGATATGCACAGTCCTGCAGCTCTCGTCCCCGAAAACAAGAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGc                                                                                                            <  2:4085649/140‑1 (MQ=255)
                            aaaCAGCTGATCGAAGCATTTTACCGGGAGGATGCGTCTGCGGCACAAAACCCCCCTCAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGc                                                                                                            <  2:4492213/140‑1 (MQ=255)
                              agagCTGATCGAAGCATTTTACCGGGATGATGCGTGTGAGGCACAAACGCCCCCTGAACCTGATATGGAAATTCCTGCAGCTATCTTCCCACAAAAAAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCa                                                                                                          <  2:3303634/140‑1 (MQ=255)
                                 gCTGTTCGAAGCTTTTTACCGGGATGATGCTTCGGCTCAAAAAAAGCCCCCAGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCATAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                       <  2:807323/140‑1 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATCATGCGTGTGAGGCAAAAAAGCCCCCTGACGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                       <  2:1828816/140‑1 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAATCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                   >  2:4149444/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                   >  1:3532084/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                   >  1:1287470/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                   >  1:2818555/1‑140 (MQ=255)
                                      tCGAAGCATTTTACCGGGATGTTGCGTCTGAGGCACAAAACCCCCCTGAAGATGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGc                                                                                                  <  2:2294511/140‑1 (MQ=255)
                                        gAAGCATTTTACCGTGATGCTGCGTCCCAGGCCCAAACGCCCCCTCACGCTGATATGGAAATTCCCCCAGCTCACGAACCAGAAAACAATAATACACCCGACCGGCAATCACACGCAGAGCCTATCGCCATTATTGGCAt                                                                                                <  2:1051605/140‑1 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                              >  1:1627168/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                              >  2:4556003/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                              >  2:4397737/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                              >  1:4442933/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGAGGCGTCTGAGGCAAAAACCCCCCCTCACGCTGATATGGAATTTCCTGCAGCTCTCGGACCCTAAAACAAAAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                              <  1:4048945/140‑1 (MQ=255)
                                          aGCATTTCACCGGTTTGTGGGGTCGGAGCCAAAAACGCCCCCTTAAGCTTATATGTAATTTCCGCCAGCTCTCGTACCATAAAAAAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                              <  1:1927554/140‑1 (MQ=255)
                                              ttttCCCGGTATGATGCGTGAGAGCAACAAACCCCCCCCCACGATCATCTTGAAATTCAGCCAGACCCCGAACAACAAAAAAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTgg                                                                                          <  1:4296805/140‑1 (MQ=255)
                                                 tACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCGGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATAATTGGCATGAGTGGCAg                                                                                       >  2:2831840/1‑140 (MQ=255)
                                                  aCCGGGAGGATGCGTGTCTGGCACAAACGCCCCCTGACGCTGATATGGAATGTCCTGCAGCTCTCGTACCAGAAAAAATTAATATCCGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAgg                                                                                      <  2:658473/140‑1 (MQ=255)
                                                   cggggATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGt                                                                                     <  2:4238816/138‑1 (MQ=255)
                                                      ggATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATCTGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGGATGAGTGGCAGGTTTc                                                                                  >  1:319975/1‑140 (MQ=255)
                                                        ttgtggCGTCTGCGCCACAAACGCCCCCTTAAGCTGATATGGAACTTCCTGCAGCTCTCGTACCACAAAACAATAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                <  1:2925233/135‑1 (MQ=255)
                                                        atgatgCGTCTGCGCCACAACAGCCCCCTGACGGTGATATGGAAATTCCGGCCGCTCGCGTACCACAAAAAAATAATATCAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                <  1:421193/140‑1 (MQ=255)
                                                        atgatgCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCAGGAGTGGCAGGTTTCCt                                                                                >  2:1525520/1‑140 (MQ=255)
                                                        atgatgCCTCTGAGGCACAAAAGCCACGTTAAGCTCATATGCAAATTCCTGCAGCTCACGTACCAGAAACAATTACTAAAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                <  1:2572726/140‑1 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                        >  2:3210562/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                        >  1:5192632/1‑140 (MQ=255)
                                                                 cTGAGGCACAAAAGCCCCCTGAAGCTGATATGGACATTCCTGCAGCTCTCGTACCCGAAAACAATATTATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCAGTATTGGCATGAGTGGCAGGTTTCCTCAAGCGGAt                                                                       >  2:2476322/1‑140 (MQ=255)
                                                                  tgcggCAAAAAAGCCCCCTGACGCTGATATGGAAATTCGTGCAGCTCTCGTACCACAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                      <  1:1414953/137‑1 (MQ=255)
                                                                  tgcgccacaAACGCCCCGTGAAGCTGATATGCAAATTCGTGCAGCTCTCGTACCACAAAACAATATTATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                      <  1:4503271/135‑1 (MQ=255)
                                                                  tGAGGAACAAAAGCCCCCTGAATCTGATAGGGAAATTCCTGCAGCTCTCGTACCACAAAAAAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                      <  2:3483036/140‑1 (MQ=255)
                                                                  tGAGGAACAAAAGCCCCCTGAAGCTTATATGGAAATTCCTGCAGCTCTCTTACCACAAAACACTAATATCCGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                      <  1:41705/140‑1 (MQ=255)
                                                                  ggAGGCACAAAAGCCCCTTGAAGCTTATATGTAAATTCCTGCAGCTCTCGGACCAGAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                      <  2:1989227/139‑1 (MQ=255)
                                                                   gcggaacaAAAGCCCCCTGAATGTTATATGTAATTGCGGGCAGCTCTCGTACCAGAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTc                                                                     <  1:1481768/135‑1 (MQ=255)
                                                                         caACAGCCCCCTGAAGCGGATAGGGAAATTCCTGCAGCTGTCGTACCAGAAAACCATAATATCCGCTACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCa                                                               <  2:4202387/140‑1 (MQ=255)
                                                                          aaaaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAt                                                              >  2:2591931/1‑140 (MQ=255)
                                                                           acaGCCCCGGGAAGCTTATATGCAATTGCCTGCAGCTCGCGTACAACAAAACATTAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                             <  1:1872526/138‑1 (MQ=255)
                                                                           aaaGCCCCCTCAAGCTGATATGGAAATTCCTGCAGCTATCGTCCCTGAAAACAGTAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                             <  2:2033389/140‑1 (MQ=255)
                                                                           aaaGCCCCATTAAGCTTATATGCAAATTCCTGCAGATATCGTACCAGAAAAAAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGAAGGTTTCCTCAAGCTGATTCGGTCCATg                                                             <  2:3686345/140‑1 (MQ=255)
                                                                           aaaCCCCCCGGATGCTGATATGGAAATACCGGCAGCTGTCGTCCCACAAACCAATAAAATACGCGACCGGCAATCTCCCGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCCAGCTGATTCGGTCCATg                                                             <  1:2471167/140‑1 (MQ=255)
                                                                            aaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGa                                                            >  2:1545589/1‑140 (MQ=255)
                                                                             accccccTGAAGCTGATATGCAAATTCCTGCAGCTATCGTACCACAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAg                                                           <  1:5045986/138‑1 (MQ=255)
                                                                             aGCCCCCTGAACCTGATATGTAATTTCCTGCAGCTCTCGTACCACAAAAAAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAg                                                           <  1:1375382/140‑1 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTAt                                                       >  1:3630205/1‑140 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTAt                                                       >  2:4047428/1‑140 (MQ=255)
                                                                                     tcaGCTGATATGGAAATTCCTGCAGCTGTCGTACCACAAAACACTAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCCAGCTGATTCGGTCCATGAGCTATGGGa                                                   <  2:557058/138‑1 (MQ=255)
                                                                                     taaGCTGATATGTAAATTCCTGCAGCTATCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGa                                                   <  2:5187584/139‑1 (MQ=255)
                                                                                     gAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGa                                                   <  2:5064718/140‑1 (MQ=255)
                                                                                     gAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGa                                                   <  2:1870743/140‑1 (MQ=255)
                                                                                     gAAGCTGATATCGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGa                                                   <  2:3863098/140‑1 (MQ=255)
                                                                                       aGCTGATATGGAAATTCCTGCAGCCCTCGAACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACa                                                 <  2:2637336/140‑1 (MQ=255)
                                                                                           gATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCCTTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTCTGGGACActct                                             >  2:2954116/1‑140 (MQ=255)
                                                                                           gATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCt                                             >  2:405060/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAGTCGGTCCATGAGCTATGGGACAATCTg                                            >  1:2233384/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCa                                                                                                          >  1:2406663/1‑78 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                            >  1:613612/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTACAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                             >  1:335478/1‑123 (MQ=255)
                                                                                                      tCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCa                                  >  1:2641408/1‑140 (MQ=255)
                                                                                                      tCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCa                                  <  2:1627168/140‑1 (MQ=255)
                                                                                                         tGCAGCTCTCGTACCAGACAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCAGTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAGGAGCTATGGGACAATCTGAAAAACGGCAAAc                               >  2:2060928/1‑140 (MQ=255)
                                                                                                            aGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACAtt                            <  2:3381299/140‑1 (MQ=255)
                                                                                                             gCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTg                           <  1:5012495/140‑1 (MQ=255)
                                                                                                                ctcGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTAt                        >  1:989426/1‑140 (MQ=255)
                                                                                                                ctcGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTAt                        >  1:3894071/1‑140 (MQ=255)
                                                                                                                ctcGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTAt                        >  2:2797392/1‑140 (MQ=255)
                                                                                                                      ccAGAAAAAAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGa                  <  2:4741899/140‑1 (MQ=255)
                                                                                                                         gAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGACAAACGGCAAACATTGTATCTCTGACAt               >  2:2992960/1‑140 (MQ=255)
                                                                                                                          aaaaCAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATc              >  2:4992202/1‑140 (MQ=255)
                                                                                                                                    tataAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAc    >  1:2097565/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
ATTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAACGC  >  NZ_CP034943/1800738‑1801011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: