Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,936,643 A→C 5.3% D3536E (GAT→GAG) ‡ EO946_RS09735 ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,936,6430AC 5.3% 1136.4 / 13.0 407D3536E (GAT→GAG) ‡EO946_RS09735non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (159/212);  new base C (31/4);  total (190/217)
Fisher's exact test for biased strand distribution p-value = 1.34e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

GTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTAACA  >  NZ_CP034943/1936506‑1936782
                                                                                                                                         |                                                                                                                                           
  ttCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGAATGAACTTGGGTACCGTCGTGTTTGGAAGTGCCTCTCCGGCAAATAACAGAAGACCCAGCCGTACAGCATCCCcataat                                                                                                                                         >  2:3608583/1‑140 (MQ=255)
   tCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGGCTCTCCACCAATTAAAATATGACGAACCGGTACTCCTTCCCcaacaaa                                                                                                                                        >  1:2460940/1‑138 (MQ=255)
     ggTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTg                                                                                                                                      <  1:4879275/140‑1 (MQ=255)
       ttGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCa                                                                                                                                    <  2:2706572/140‑1 (MQ=255)
          gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGGGTCTCCTCCCATTAACTAATGACGAAGCGGTACTCCTTGCCCATCAAGTGCACCt                                                                                                                                 >  1:3920758/1‑140 (MQ=255)
                   cATTTTTGTTTGCAGGTGCTTCTCTATCCGGGCCAAATAAGTGTATGAACTTGGTTAACGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTGCCCTTCATCTGCAGCTACCtgcagc                                                                                                                        <  2:5072418/140‑1 (MQ=255)
                    aTTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCt                                                                                                                       <  2:4596999/140‑1 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTTGCTCTCCTCCAATTAACATATTACGAAGCGGTAATCCATACCCAACAAATGCAGCTACCAGCAGCtt                                                                                                                      >  2:5099893/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGTCGAAGCGGTACTCCTTCCCCAACAACTGCAGCTACCACCACCtt                                                                                                                      >  1:3850744/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAACTGCCGCTCCTCCAAATAACAGATGACGAAGCGGTACACCTTACCCAACAACTTCAGCAACCACCAGCtt                                                                                                                      >  2:1346434/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAAATAAAAGATGACGAAGCGGTACTCCTTACCCAACAACTGCAGCTACCagcagcgt                                                                                                                      >  2:812589/1‑138 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCTCCTCCAATTAACAGATTAAGAAGCGGTACTACTTACCCATCATCTGCAGCTAACAGCAGCtt                                                                                                                      >  2:4366787/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCGCCAATTAACAGATTACGAAGCGGTACTACTTCCCCAACATCAGCAGCTACCACCACCtt                                                                                                                      >  2:185355/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCGCCAATTAAAAGAAGACCAAACGGTACACCTTCCACATCAAGTGCACCTACAaccagcat                                                                                                                      >  2:4746301/1‑138 (MQ=255)
                       tgtgATGGCATGTGCTGCTCTATCCTCGCCAAATAAGTGTATGAAATTGGTTACCGTCGTGTTTGGAATTGCCACTGCTCCACTTAACCGAAGAAGGAGCGGGACTCCGTCCCCATGATAAGCAGCGACCACCAGATTCa                                                                                                                    >  2:2926837/1‑140 (MQ=255)
                       tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTGCCGCGCCTCCAATTAACAGATTACGAAGCCGGACTACTTACCCATCATCTGCACCTACCAGCAGCATCa                                                                                                                    >  2:2879713/1‑140 (MQ=255)
                       tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTTCCTCGCCTCCAAATAACAGATAACCAAGCGGTACGCCTTCCCCATCATCTGCAGCAACCAGCAGCATCa                                                                                                                    >  2:1134733/1‑140 (MQ=255)
                        gtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCaa                                                                                                                   <  1:2085034/140‑1 (MQ=255)
                         tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTGCTACAAATAACAGATGACGAAGCGGTACTCCTTCCCCCACATATGCAGCTACCAGCAGCATCaaa                                                                                                                  >  2:3328256/1‑140 (MQ=255)
                           gagacAGGTGCTGCTCTATCCGCGCCAAATAAGTGGATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACCCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATg                                                                                                                <  2:4211662/136‑1 (MQ=255)
                             ggCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATggg                                                                                                              <  2:183312/140‑1 (MQ=255)
                             ggCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATGAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATggg                                                                                                              <  2:2587962/140‑1 (MQ=255)
                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCACCTGCAAGTAAAAGATGAAGAATCCGTAATACATCCCCATAATATGCAGCAACCAGAAGCTAAAAATgtgc                                                                                                             >  2:4936/1‑140 (MQ=255)
                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGGCTCGCCTCCAAATAACAGATTTCGAAGCGGTACAACTTCCCCATCATCTGCAGCTAACAGCAGCTTAAAATgggc                                                                                                             >  1:1290468/1‑140 (MQ=255)
                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggc                                                                                                             >  2:3264327/1‑140 (MQ=255)
                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCTACAATTAAAACATTACGAAGCCGTAATCCTTCCCCATCAACTGCACCTACCAGCAGCTTCAAATgggc                                                                                                             >  2:2790107/1‑140 (MQ=255)
                                aGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggcgg                                                                                                           <  1:57657/140‑1 (MQ=255)
                                 ggTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggcggg                                                                                                          <  1:1201821/140‑1 (MQ=255)
                                  gTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGGAGTTCCTCTGCTCCCAATAACAGATGAAGAAGCGGGACTCCTTCCCCATCATCAGCAGCGACCAGCAGCTTCAAATgggcgggc                                                                                                         >  1:4739598/1‑140 (MQ=255)
                                  gTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTTCCTCTCCTCCAAATAACAGATGACGAAGCCGTACTCCGTACCCAACAAAAGCAGCTACCAGCAGCATCAAAAgggtgggc                                                                                                         >  2:2213465/1‑140 (MQ=255)
                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                                                   <  1:3017354/140‑1 (MQ=255)
                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                                                   <  1:2570393/140‑1 (MQ=255)
                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                                                   <  1:2884268/140‑1 (MQ=255)
                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                                                   <  1:1392607/140‑1 (MQ=255)
                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCTGTACTCCAATTAACAGATTGCGAAGCGGTAATCCTTACCCCTCATCTGCAGCTACCAGCAGCTTAAAATTGGCGGGCGTCCCGTAGGt                                                                                              >  2:3179935/1‑140 (MQ=255)
                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCCACATCTGCAGCTACCAGCAGCTTCAAAAGGGCGGGCGTCCCGTCGGt                                                                                              >  2:2984255/1‑140 (MQ=255)
                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                                              <  2:2350022/140‑1 (MQ=255)
                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTAATCCTTGCCCATCATCTGCAGCTACCAGCAGCGTCAAATGGGCGGGCGTCCCGGCGGt                                                                                              >  2:3739792/1‑140 (MQ=255)
                                                gcgcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTAc                                                                                           <  2:3534452/140‑1 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTGCCATTAACAGATTTCGAAGCGGTAATCCTTACCCCACAAATGCAGCAACCACCAGCTTAAAAATGGCGGGCGTGCCGGCGGGTaagg                                                                                         >  1:213760/1‑137 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCACCTCCAAATAACAGATGACGAAGCGGTACTCCATGCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTGCCGTCGGTTAAGt                                                                                         >  2:2040/1‑140 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCGCTCCTCCAATTAACAGAAGACCAAGCGGTACTCCTTACCCATCAACAGCAGCTACCAGCAGCGTCAAATTGGCGGGCGGCCCGTCGGGTAcct                                                                                         >  1:4792713/1‑138 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGTAGTGGCGCGGCGCCAAATAACACATGTCCAAGCGGTACTCCGTCCCCAAAATCTTCAGCTACCACCACCGTGCAAAGGGCGGGCGGCCCGTCGGGTACGt                                                                                         >  1:4346581/1‑140 (MQ=255)
                                                     aaaTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcag                                                                                                                            <  1:3274931/102‑1 (MQ=255)
                                                       aTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATg                                                                                    <  2:2092922/140‑1 (MQ=255)
                                                       aTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATg                                                                                    <  2:1536422/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTCACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATTGGCGGGCGTCCCGTAGGTTACGTCAATGCGATg                                                                               <  1:2225639/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  2:1013683/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  1:4143223/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  2:2855363/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  1:1943766/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  1:2674812/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCACGTCGGTTACGTCAATGCGATg                                                                               <  1:2317117/140‑1 (MQ=255)
                                                              tATGTACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGGTACGTCAATGCGATGCt                                                                             <  1:63001/140‑1 (MQ=255)
                                                                tGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGAAGAAGCCGGACTCCTTCCCCAAAATCTGCAGCTACCCGCAGCGTCAAATTGGCGGGCGGCCCGTCGGTTACGGCAATGCGATGCTGt                                                                           >  2:55485/1‑140 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:4202532/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:5045574/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:3920758/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:381021/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:1007181/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGCACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:2962524/140‑1 (MQ=255)
                                                                    cTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGAt                                                                       <  2:1935427/140‑1 (MQ=255)
                                                                        gTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTa                                                                   <  1:4936/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  2:3037800/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:1632542/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:4451863/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATCACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGAAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:2790107/140‑1 (MQ=255)
                                                                         ttACCGTAGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  2:1483016/140‑1 (MQ=255)
                                                                               tcgtTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCAt                                                            <  1:1562578/140‑1 (MQ=255)
                                                                               tcgtTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCAt                                                            <  1:3474350/140‑1 (MQ=255)
                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                                                     <  1:896563/140‑1 (MQ=255)
                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                                                     <  1:3677719/140‑1 (MQ=255)
                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                                                     <  1:3582597/140‑1 (MQ=255)
                                                                                        gAAGTGCCTATCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCAACATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCTGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCg                                                   <  2:3382874/140‑1 (MQ=255)
                                                                                            tGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCAt                                               <  1:1975261/140‑1 (MQ=255)
                                                                                                   cctccAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGacac                                        <  2:3857903/140‑1 (MQ=255)
                                                                                                      ccAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAATGCCTCGCCATCAGACACCGc                                     >  1:5135685/1‑140 (MQ=255)
                                                                                                      ccAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGc                                     <  2:44211/140‑1 (MQ=255)
                                                                                                              cAGATGACGAATCGGTACTCCTTCCCCATGATCTGCAGCTACCATCAGCTTCAAAAGGGCGGGCGTCCCGTAGGTTACGTCAATGCGATGATGTCGATACTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAg                             <  1:4393721/140‑1 (MQ=255)
                                                                                                              cAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAg                             <  1:3184177/140‑1 (MQ=255)
                                                                                                                 aTGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGac                          >  1:373877/1‑139 (MQ=255)
                                                                                                                 aTGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGaa                          >  1:1513820/1‑140 (MQ=255)
                                                                                                                  tGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAAc                         <  1:4608228/140‑1 (MQ=255)
                                                                                                                        aGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                                              >  2:72806/1‑65 (MQ=255)
                                                                                                                        aGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                                              <  1:72806/65‑1 (MQ=255)
                                                                                                                         gCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAAc                  <  2:2135760/140‑1 (MQ=255)
                                                                                                                         gCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAAc                  <  1:1783733/140‑1 (MQ=255)
                                                                                                                              aCTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGt             <  2:695237/140‑1 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:1597927/75‑1 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:481211/75‑1 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTcc       >  2:1200692/1‑140 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTcc       >  2:1749207/1‑140 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTcc       >  1:3095088/1‑140 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTcc       >  1:4027028/1‑140 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGAACCCGCCTCTTTAGGAACGATAAACAAGGTATGTcc       >  1:4984795/1‑140 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGCCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAgg                            <  1:3800718/119‑1 (MQ=255)
                                                                                                                                     ccccATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCt      <  2:4475421/140‑1 (MQ=255)
                                                                                                                                       ccATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTaa    >  2:4057331/1‑140 (MQ=255)
                                                                                                                                       ccATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTaa    >  2:3133623/1‑140 (MQ=255)
                                                                                                                                         atcatcTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTaaca  >  2:88040/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTAACA  >  NZ_CP034943/1936506‑1936782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: