Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,936,644 T→A 9.6% D3536V (GAT→GTT) ‡ EO946_RS09735 ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,936,6440TA 9.6% 1189.7 / 17.9 407D3536V (GAT→GTT) ‡EO946_RS09735non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (141/214);  new base A (47/2);  total (190/217)
Fisher's exact test for biased strand distribution p-value = 5.90e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

GTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTAACA  >  NZ_CP034943/1936506‑1936782
                                                                                                                                          |                                                                                                                                          
  ttCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGAATGAACTTGGGTACCGTCGTGTTTGGAAGTGCCTCTCCGGCAAATAACAGAAGACCCAGCCGTACAGCATCCCcataat                                                                                                                                         >  2:3608583/1‑140 (MQ=255)
   tCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGGCTCTCCACCAATTAAAATATGACGAACCGGTACTCCTTCCCcaacaaa                                                                                                                                        >  1:2460940/1‑138 (MQ=255)
       ttGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCa                                                                                                                                    <  2:2125283/140‑1 (MQ=255)
       ttGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCa                                                                                                                                    <  1:3579630/140‑1 (MQ=255)
                   cATTTTTGTTTGCAGGTGCTTCTCTATCCGGGCCAAATAAGTGTATGAACTTGGTTAACGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTGCCCTTCATCTGCAGCTACCtgcagc                                                                                                                        <  2:5072418/140‑1 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTTGCTCTCCTCCAATTAACATATTACGAAGCGGTAATCCATACCCAACAAATGCAGCTACCAGCAGCtt                                                                                                                      >  2:5099893/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGTCGAAGCGGTACTCCTTCCCCAACAACTGCAGCTACCACCACCtt                                                                                                                      >  1:3850744/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGACGAATCGGTACTACTTACCCAACATATGCAGCTAACACCAGCtt                                                                                                                      >  2:3380064/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCGCCAAATAAAAGATGACGAACCGGTACTCCTTACCCAACATCTTCAGAGACCCCCAGCtt                                                                                                                      >  2:4202532/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGACGTGCCTCTCGTGCCAATAACAGAATCAGCACCGGTAATCCTTCCCCAGCATCAGAAGCTACCAGCAGCtg                                                                                                                      >  2:1815366/1‑139 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTGCAATTAAAAGATTACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCtt                                                                                                                      >  2:3129497/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAAATAAAAGATGACGAAGCGGTACTCCTTACCCAACAACTGCAGCTACCagcagcgt                                                                                                                      >  2:812589/1‑138 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGGTGCTCCAAATAAAAGATTAACAAACGGTACGACATCCCCATAAACAGCAGCTAACAACAACtt                                                                                                                      >  2:4393721/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGGCTCTCCTCCAATTAAAAGATGACGAAGCGGTACTCCTTCCCCATAATATTCAGCTACCAGCACCtt                                                                                                                      >  1:566906/1‑140 (MQ=255)
                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCGCCAATTAACAGATTACGAAGCGGTACTACTTCCCCAACATCAGCAGCTACCACCACCtt                                                                                                                      >  2:185355/1‑140 (MQ=255)
                       tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTGCCGCGCCTCCAATTAACAGATTACGAAGCCGGACTACTTACCCATCATCTGCACCTACCAGCAGCATCa                                                                                                                    >  2:2879713/1‑140 (MQ=255)
                       tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAAATAACAGATGACGAAGCGGTACTAATTACCCATCATATGACACTAACAGCACCTTCa                                                                                                                    >  2:4056267/1‑140 (MQ=255)
                         tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCCATTAACAGATTAAGAAGCGGTACTCCTTCCCCATCATCTGCAGCAAACAGCAGCATCaaa                                                                                                                  >  2:3025135/1‑140 (MQ=255)
                             ggCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATggg                                                                                                              <  2:2182713/140‑1 (MQ=255)
                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCACCTGCAAGTAAAAGATGAAGAATCCGTAATACATCCCCATAATATGCAGCAACCAGAAGCTAAAAATgtgc                                                                                                             >  2:4936/1‑140 (MQ=255)
                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGGCTCGCCTCCAAATAACAGATTTCGAAGCGGTACAACTTCCCCATCATCTGCAGCTAACAGCAGCTTAAAATgggc                                                                                                             >  1:1290468/1‑140 (MQ=255)
                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGACGTGCCTCTCCGCCAATTAACAGATGACGAAGAGGTACTCCTTCCCCATAATCTGCAGAGACCAGCAGCTTCAAATgggg                                                                                                             >  2:2547595/1‑139 (MQ=255)
                                 ggTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggcggg                                                                                                          <  1:1201821/140‑1 (MQ=255)
                                  gTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggcgggc                                                                                                         <  1:3072427/140‑1 (MQ=255)
                                  gTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGGAGTTCCTCTGCTCCCAATAACAGATGAAGAAGCGGGACTCCTTCCCCATCATCAGCAGCGACCAGCAGCTTCAAATgggcgggc                                                                                                         >  1:4739598/1‑140 (MQ=255)
                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                                                   <  1:2884268/140‑1 (MQ=255)
                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                                                   <  1:1392607/140‑1 (MQ=255)
                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCTGTACTCCAATTAACAGATTGCGAAGCGGTAATCCTTACCCCTCATCTGCAGCTACCAGCAGCTTAAAATTGGCGGGCGTCCCGTAGGt                                                                                              >  2:3179935/1‑140 (MQ=255)
                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCGCTGCTCCAATTAAAAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCGACCCGCAGCGTCAACTGGGCGGGCGGCCCCTCGGt                                                                                              >  2:4534838/1‑140 (MQ=255)
                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                                              <  2:2350022/140‑1 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTGCCATTAACAGATTTCGAAGCGGTAATCCTTACCCCACAAATGCAGCAACCACCAGCTTAAAAATGGCGGGCGTGCCGGCGGGTaagg                                                                                         >  1:213760/1‑137 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCGCTCCTCCAATTAACAGAAGACCAAGCGGTACTCCTTACCCATCAACAGCAGCTACCAGCAGCGTCAAATTGGCGGGCGGCCCGTCGGGTAcct                                                                                         >  1:4792713/1‑138 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGTAGTGGCGCGGCGCCAAATAACACATGTCCAAGCGGTACTCCGTCCCCAAAATCTTCAGCTACCACCACCGTGCAAAGGGCGGGCGGCCCGTCGGGTACGt                                                                                         >  1:4346581/1‑140 (MQ=255)
                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCTCCAATTAACAAATGACGAAGCGGTACTCCTTGCCCAACATCTGCAGCTACCAGCAGCTTCAAAATGGCGGGCGTCCCGTCGGTTACGt                                                                                         >  1:4969860/1‑140 (MQ=255)
                                                     aaaTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcag                                                                                                                            <  1:3274931/102‑1 (MQ=255)
                                                       aTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATg                                                                                    <  2:1536422/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTCACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATTGGCGGGCGTCCCGTAGGTTACGTCAATGCGATg                                                                               <  1:2225639/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  2:4440865/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  1:2674812/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  1:4143223/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  2:1013683/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                                                                               <  1:1943766/140‑1 (MQ=255)
                                                            tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCACGTCGGTTACGTCAATGCGATg                                                                               <  1:2317117/140‑1 (MQ=255)
                                                              tATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                                                                             <  1:4876851/140‑1 (MQ=255)
                                                                tGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGAAGAAGCCGGACTCCTTCCCCAAAATCTGCAGCTACCCGCAGCGTCAAATTGGCGGGCGGCCCGTCGGTTACGGCAATGCGATGCTGt                                                                           >  2:55485/1‑140 (MQ=255)
                                                                tGAACTTGGTTACCGTCGTTTTTTGAACTTCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCAACCACTGCAGCTACCAGCAGCGTCAAAAGGGCGGGCGTCCCCTCGGTTACGGCAATGCGAAGCTGt                                                                           >  2:1581616/1‑140 (MQ=255)
                                                                tGAACTTGGTTACCGTCGTTTTTGGAAGTTCCTCTCCTCCAATTAAAAGAAGACGAAGAGGTACTCCTTCCCCATCATAATCAGCGACCCGCAGCGTAAAAAGGGCGGGCGTCCCGTCGGTTACCTCAATGCGAAGcggg                                                                           >  2:2770908/1‑137 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:5045574/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:3919112/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:1007181/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:2855155/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:4202532/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:3675673/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCACGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:3005936/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGCACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  1:2962524/140‑1 (MQ=255)
                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGCTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:1343843/140‑1 (MQ=255)
                                                                    cTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGAt                                                                       <  2:1935427/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:3966671/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:1632542/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCGTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:256113/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCGTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:4925272/140‑1 (MQ=255)
                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATCACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGAAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  1:2790107/140‑1 (MQ=255)
                                                                         ttACCGTAGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                                                  <  2:1483016/140‑1 (MQ=255)
                                                                               tcgtTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCAt                                                            <  1:3474350/140‑1 (MQ=255)
                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                                                     <  1:896563/140‑1 (MQ=255)
                                                                                            tGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCAt                                               <  1:1975261/140‑1 (MQ=255)
                                                                                                    ctccAATTAAAAGATGACGAAGCGGTACTCCTTCCCCCTCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACTcc                                       <  2:2086689/140‑1 (MQ=255)
                                                                                                      ccAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAATGCCTCGCCATCAGACACCGc                                     >  1:5135685/1‑140 (MQ=255)
                                                                                                      ccAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGc                                     >  1:716699/1‑140 (MQ=255)
                                                                                                         aTTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCCTCAGACACCGCctc                                  >  2:2697129/1‑140 (MQ=255)
                                                                                                              cAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAg                             <  1:3184177/140‑1 (MQ=255)
                                                                                                              cAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAg                             <  1:4393438/140‑1 (MQ=255)
                                                                                                                 aTGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATg                                                                                    <  2:4727777/82‑1 (MQ=255)
                                                                                                                 aTGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGac                          >  1:373877/1‑139 (MQ=255)
                                                                                                                 aTGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGaa                          >  2:111512/1‑140 (MQ=255)
                                                                                                                 aTGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGaa                          >  1:1513820/1‑140 (MQ=255)
                                                                                                                  tGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAAc                         <  1:4608228/140‑1 (MQ=255)
                                                                                                                   caCGAAGCGGTACTCCTTACAAATAAACTGCAGATAAAACCATCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACg                        >  2:3483085/2‑140 (MQ=255)
                                                                                                                      gAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGACGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATa                     >  2:3733618/1‑140 (MQ=255)
                                                                                                                        aGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                                              >  2:72806/1‑65 (MQ=255)
                                                                                                                        aGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATaaa                   <  1:391709/140‑1 (MQ=255)
                                                                                                                        aGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATaaa                   <  2:1197839/140‑1 (MQ=255)
                                                                                                                         gCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGTGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAAc                  <  2:2961550/140‑1 (MQ=255)
                                                                                                                         gCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAAc                  <  1:1783733/140‑1 (MQ=255)
                                                                                                                           ggTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACaa                <  2:2022749/140‑1 (MQ=255)
                                                                                                                            gTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGGATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAg               >  1:2056867/1‑140 (MQ=255)
                                                                                                                              aCTCCTTCCCCATCATCTGCAGCTACGAGCAGCTTCAATTGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGt             <  2:4281153/140‑1 (MQ=255)
                                                                                                                              aCTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGt             <  2:695237/140‑1 (MQ=255)
                                                                                                                              aCTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGt             <  2:3244605/140‑1 (MQ=255)
                                                                                                                              aCTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGt             <  2:3850744/140‑1 (MQ=255)
                                                                                                                              aCTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGt             <  2:3326047/140‑1 (MQ=255)
                                                                                                                                tCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTAt           <  1:1561192/140‑1 (MQ=255)
                                                                                                                                    ttcccATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTcc       >  1:4426018/3‑140 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                                                        <  2:481010/75‑1 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTcc       >  1:618415/1‑140 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGCCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAgg                            <  1:3800718/119‑1 (MQ=255)
                                                                                                                                    tCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGCCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAgg                            >  2:3800718/1‑119 (MQ=255)
                                                                                                                                         atcatcTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAATGTATGTCCTaaca  >  2:164246/1‑140 (MQ=255)
                                                                                                                                         atcatcTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTaaca  >  2:2923754/1‑140 (MQ=255)
                                                                                                                                         atcatcTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTaaca  >  2:88040/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAGGAACGATAAACAAGGTATGTCCTAACA  >  NZ_CP034943/1936506‑1936782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: