Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,073,288 A→T 5.5% G335G (GGT→GGA EO946_RS10385 ← NTTRR‑F1 domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,073,2880AT 5.5% 409.8 / 6.2 137G335G (GGT→GGAEO946_RS10385NTTRR‑F1 domain
Reads supporting (aligned to +/- strand):  ref base A (55/73);  new base T (5/4);  total (60/77)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TTGCTCCTGTTGATCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCA  >  NZ_CP034943/2073149‑2073426
                                                                                                                                           |                                                                                                                                          
ttGCTCCTGTTGATCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTCCTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgca                                                                                                                                            >  2:3645494/1‑140 (MQ=255)
ttGCTCCTGTTGATCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTCCTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgca                                                                                                                                            >  1:212419/1‑140 (MQ=255)
ttGCTCCTGTTGATCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgca                                                                                                                                            <  2:1387341/140‑1 (MQ=255)
gtGCTCCTGTTGATCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgca                                                                                                                                            <  1:1228945/139‑1 (MQ=255)
 tGCTCCTGTTGCGCCAGTTTCCCCCGTTGCACCAGTTACTCCCGGTGCTCCAGGTGCTCCTGTTGCTCCAGTTTCTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgcac                                                                                                                                           <  2:5071744/140‑1 (MQ=14)
 tGCTCCTGTTGATCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgcac                                                                                                                                           <  2:1926653/140‑1 (MQ=255)
           gATCCTGTTCCCCCGGTTGCACCAGTTCCCCCCGTTGCTCCCCTTGCACCAGTTGCACCATTTTCTCCCGTTTCTCCCGTTTCTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgcaccagttgatcc                                                                                                                                 <  1:3350214/140‑1 (MQ=18)
           gATCCGGTTTCCCCGGTTGCACCCGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgcaccagttgatcc                                                                                                                                 <  1:2323390/140‑1 (MQ=255)
            aTCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgcaccagttgatccc                                                                                                                                <  1:616300/140‑1 (MQ=255)
              ccTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTtgcaccagttgatcccgttgcaccagttgatcccgt                                                                                                                              >  2:4387554/1‑140 (MQ=255)
              ccTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTtgcaccagttgatcccgttgcaccagttgatcccgt                                                                                                                              >  2:3533393/1‑140 (MQ=255)
                  tttCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCt                                                                                                                          >  1:3230919/1‑140 (MQ=255)
                  gttCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCt                                                                                                                          <  1:4121752/139‑1 (MQ=255)
                   ttCCCCGGTTGCACCAGTTCCTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTc                                                                                                                         <  1:2260095/140‑1 (MQ=255)
                   ttCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGgttgcaccagttgatcccgttgcaccagttga                                                                                                                                    <  2:1062209/129‑1 (MQ=255)
                     ccccGGTTGCACCAGTTACTCCCGTTGCTCCTGTTGCTCCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCt                                                                                                                       <  2:4417725/140‑1 (MQ=255)
                          gTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGa                                                                                                                  >  1:1687668/1‑140 (MQ=255)
                          gTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGa                                                                                                                  >  1:3791963/1‑140 (MQ=255)
                          gTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGa                                                                                                                  >  1:1961952/1‑140 (MQ=255)
                           ttGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGAt                                                                                                                 >  1:1598543/1‑140 (MQ=255)
                           ttGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGAt                                                                                                                 <  1:1580314/140‑1 (MQ=255)
                                   gTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGc                                                                                                         >  2:1815266/1‑140 (MQ=255)
                                         cccGGTGCTCCAGGTGCTCCAGTGGCACCAGTCTCTCCCGGTACTCCAGTTCCTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTCCTCCCGGTGCACCAGTTGCTCCCGTTGCACCTGTTGATCCCGTTGCTCCCGt                                                                                                   <  1:2193253/140‑1 (MQ=17)
                                             ttGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCCGTTGCGCCCGTTGCACGAGTTGATCCGGTTGCTCCTGTTGCTCCGGTTGCTCCGGTGGcg                                                                                               >  1:881154/1‑139 (MQ=14)
                                             ttGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCa                                                                                               >  1:692085/1‑140 (MQ=255)
                                             ttGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGtt                                                                                                                    >  2:332746/1‑119 (MQ=255)
                                               gCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGa                                                                                                                  <  1:4103149/119‑1 (MQ=255)
                                                  ccATTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          <  1:2225622/140‑1 (MQ=255)
                                                  ccAGTTGCACGAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          <  1:4473832/140‑1 (MQ=255)
                                                  ccAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          <  2:1961952/140‑1 (MQ=255)
                                                  ccAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          <  1:5122397/140‑1 (MQ=255)
                                                  ccAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          <  1:3306074/140‑1 (MQ=255)
                                                  ccAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGATTCCCCGGTTGCACCAGTTTATCCCGTTGCACCAGTTGATCCCGTTGCTCCAGTTGATCCCGTAGATACCGTCGCTCCAGt                                                                                          >  1:1371034/1‑140 (MQ=18)
                                                  acAGTTGCACCAGTGGCACCACTTTCTCCCGTTCCGCCCGTTTCTCCCGTTGCGCCTGTTTCCCCGGTTGCACCAGTTGCTCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          <  2:1288934/139‑1 (MQ=18)
                                                              gTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGa                                                                              <  1:3418438/140‑1 (MQ=255)
                                                               tGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGAt                                                                             <  1:4248143/140‑1 (MQ=255)
                                                                      aGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGt                                                                                                   <  2:3981785/111‑1 (MQ=255)
                                                                        ttactcccgttactcccgttactcccgTTGCGGCAGTTTCACCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGGTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGa                                                                    <  2:2406375/140‑1 (MQ=255)
                                                                        ttactcccgttactcccgttactcccgTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGa                                                                    <  1:511890/140‑1 (MQ=255)
                                                                        ttactcccgttactcccgttactcccgTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGa                                                                    <  1:3522967/140‑1 (MQ=255)
                                                                        ttactcccgttactcccgttactcccgTTGCGCCAGTGTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGa                                                                    <  1:2546755/140‑1 (MQ=255)
                                                                             cctgttgctcccgttcttcccgttGCGCCTTGTGCTCCGGTTGCTGCTGTTGCTCCGGGTGCACCAGTTTATCCCGTTGCTCCTGTTGATCCCGTTGCGCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGt                                                               <  1:2981475/137‑1 (MQ=17)
                                                                             cccgttactcccgtttctcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGt                                                               <  1:3526558/140‑1 (MQ=255)
                                                                             cccgttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGt                                                               <  1:3645494/140‑1 (MQ=255)
                                                                             cccggttctccggttgctcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGGTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGt                                                               <  1:4319608/140‑1 (MQ=18)
                                                                             cccggtactcccggttctcccgttGCGCCAGGTTCCCCGGGTGCACCAGTTGATCCCGGTGCACCACTTGATCCCGGTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGt                                                               <  1:4580765/140‑1 (MQ=25)
                                                                               cgttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGt                                                                                                                     <  2:2517895/84‑1 (MQ=38)
                                                                               cgttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGt                                                                                                                     <  2:211715/84‑1 (MQ=38)
                                                                                gttactcccgttactcccgttGCTCCAGTTTCCCCGGGTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTgg                                                            <  1:4968260/140‑1 (MQ=255)
                                                                                gttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCCGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTgg                                                            >  2:2555465/1‑140 (MQ=255)
                                                                                gttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTgg                                                            <  1:3113988/140‑1 (MQ=255)
                                                                                gttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTgg                                                            <  1:4947641/140‑1 (MQ=255)
                                                                                gttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGATGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTgg                                                            <  1:4812251/140‑1 (MQ=255)
                                                                                 ttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          >  2:52964/1‑109 (MQ=255)
                                                                                 ttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          <  1:52964/109‑1 (MQ=255)
                                                                                 ttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACATGTTGGt                                                           >  1:3180016/1‑140 (MQ=255)
                                                                                 ttactcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCAGTAGCACCCGTGGCTCCGGTTGATCCAGTTGGTCCTGTTgct                                                           >  2:2658986/1‑138 (MQ=14)
                                                                                   gctccggtttctccagttGCTCCAGTTTCGCCTGTTGCACCAGTTTATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCAGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTcc                                                         <  2:3904292/139‑1 (MQ=18)
                                                                                     tcccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGGTGCTCCTGTTGATCCCGTTGCTCCCGTAGCACCAGTGGCTCCTGTTGGTCCCGTAGGACCTGTTCGTCATg                                                       >  2:68625/1‑140 (MQ=18)
                                                                                      cccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCTGTTGATCCAGTTGCTCAAATTGAGCCCGTGGCTCGGGATGATGACGTATCTCGCGTCGCACAAGTAGCTCATGTTGATCCCGTGGGGCGGGTTGGTGCTGt                                                      >  2:2883141/1‑140 (MQ=9)
                                                                                      cccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTGGCTCCTGTTGCTCCTGTTGCTCCGGTTTCTCCTGTTGGTCCTGt                                                      >  2:3846035/1‑140 (MQ=14)
                                                                                      cccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCTCCGGTGGCACCAGTTGGACCTGTTGGTCCGGTTGGTCCGGTGGGTCCTGt                                                      >  2:2219301/1‑140 (MQ=14)
                                                                                      cccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCGGTTGATCCCGTTGCTCCCGTGGCTCCAGTGGCTCCTGTTGAGCCGGTTTCTCCTGTTGGTCCTGt                                                      >  2:3616631/1‑140 (MQ=17)
                                                                                      cccgttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTGGCTCCCGTTCCACCCCTTGATCCCGGTGCTGCTGTTGATCCGGTTTCTCCCGTCGCACCAGTGGGTCCTGTTGCTCCCGTTGGGGGTGTGGGTCCGGt                                                      >  2:3168847/1‑140 (MQ=17)
                                                                                        cgttactcccggtGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAg                                                    <  1:2535709/140‑1 (MQ=255)
                                                                                         cgcacgcccgttGCGCCAGGTTCCCCGGTTCCACCAGTAGAGACCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTAGATCCCGTTGGACCTGTTGGTCCTGTAGc                                                   <  1:3949660/134‑1 (MQ=21)
                                                                                          ttactcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCt                                                  >  2:3247326/1‑140 (MQ=255)
                                                                                          ttactcccggtGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGGTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCt                                                  <  1:5080060/140‑1 (MQ=255)
                                                                                              tcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCTGTTGATCCCGTTGCTCCGGTTGCTCCCGTTGCTCCGGTAGCACCAGTTGCTCCGGTTGCTCCGGTTGCTCCGGTTGGTCCGGTTGCTcccg                                              >  2:278966/1‑138 (MQ=11)
                                                                                              tcccgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTGGCTCCGGTTGCTCCCGTTGCTCCCGTTGGTCCTGTGGGTCCGGTAGCTCCAg                                              >  2:3683504/1‑140 (MQ=17)
                                                                                                 cgttGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTg                                           >  1:2763360/1‑140 (MQ=255)
                                                                                                      cgcCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTcc                                                                                                      >  2:2326495/1‑76 (MQ=255)
                                                                                                      cgcCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAcca                                      <  1:4342402/140‑1 (MQ=255)
                                                                                                       gcCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccag                                     <  1:1498461/140‑1 (MQ=255)
                                                                                                                  ccggcgcaccactcgaccccgttgcaccagttgctccccttCCTCGTGGCGATCGAGTTGCGTGCGCAGCAACAGGGGCTCCAGTGCCACCAGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccagtggctccagtg                          <  1:5164652/135‑1 (MQ=9)
                                                                                                                     ttgcaccagttgatcccgttgcaccagttgatcccgTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccagtggctccagtggct                       >  2:830577/1‑140 (MQ=255)
                                                                                                                     gtgcaccagttgatcccgttgcacgagttgatcccgtTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccagtggctccagtggct                       <  1:4048857/139‑1 (MQ=255)
                                                                                                                      tgcaccagttgatcccgttgcaccagttgatcccgtTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACcagtggctccagtggctc                      >  2:4174407/1‑140 (MQ=255)
                                                                                                                      tgaaccagttgatccctttgaacgagttgatcccgttgcTCGTGTTGTACCCGTTGAACCCGTGGAACCATTGGATCGTGTTGTTCGCGTTGGACCTGTTGGTCCTGTAGGACCAGTTCAACcagtggctaaagtgcctc                      >  2:4750030/4‑140 (MQ=12)
                                                                                                                      tacgccagttgctccggttgcaccagttgttcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAcca                                      >  2:3733478/5‑124 (MQ=17)
                                                                                                                      tacgccagttgctccggttgcaccagttgttcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAcca                                      <  1:3733478/120‑1 (MQ=17)
                                                                                                                        caccagttgatcccgttgcaccagttgatcccgttgCTCCTGTTGATCCCGTTGCTCCCGTCGATCCGGTGGGTCCTGTTGATCCGGTTGGGCCTGTTGGTCCTGTCGCTGAAGTTTCAccagtggctccaggggcgcgg                    >  2:3772611/1‑136 (MQ=18)
                                                                                                                          cctgttgctcccgttgcaccatttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTggctccagtggctccagt                  <  1:3317307/137‑1 (MQ=255)
                                                                                                                          ccagttgatcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTggctccagtggctccagt                  <  1:1013143/140‑1 (MQ=255)
                                                                                                                             gttgatcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTAc               >  2:1610406/1‑140 (MQ=255)
                                                                                                                             gttgatcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTAc               >  2:4777899/1‑140 (MQ=255)
                                                                                                                              ttgatcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCTCCAGTGGCTCCAGTGGCTCCAGTTACt              <  1:2683359/140‑1 (MQ=255)
                                                                                                                              ttgatcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACt              <  1:2346847/140‑1 (MQ=255)
                                                                                                                              ttgatcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACt              >  2:2784992/1‑140 (MQ=255)
                                                                                                                               tgatcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTc             >  2:3037031/1‑140 (MQ=255)
                                                                                                                                  tcccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGt                                                                                          >  2:4734516/1‑60 (MQ=255)
                                                                                                                                   cccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccagtggctccagtggct                       <  1:4748188/126‑1 (MQ=255)
                                                                                                                                   cccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCAccagtggctccag                            >  1:837828/1‑121 (MQ=255)
                                                                                                                                   cccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGt         >  2:839081/1‑140 (MQ=255)
                                                                                                                                   cccgttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGt         >  2:1119258/1‑140 (MQ=255)
                                                                                                                                       ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTGGCACCAGTGGCGCCTGTTGATCCCGTTGGTCCTGTTGGTCCTGTAGCTCCAGTTGCACCCGTGGCTCCCGTGGCTCCAGTTGCTCCCGTTgcg     >  2:1640953/1‑140 (MQ=18)
                                                                                                                                       ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCCGTTACTCCCGATgcg     >  2:2978692/1‑140 (MQ=255)
                                                                                                                                       ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTGCCGTTgcg     >  2:3176554/1‑140 (MQ=255)
                                                                                                                                       ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCACTGGCTCCTGTTGATCCAGTGGCTCCTGTAGCTCCTGTAGCTCCAGTTGAACCAGTGGCTCCAGGGGCTCCCGTCACTCCCGTTgcg     >  2:3077175/1‑140 (MQ=21)
                                                                                                                                        tgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGGTCCAGTTACTCCCGTTgcgc    >  2:4087340/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTGCTCCTGTTGATCCTGTTTCCCCGGTTGCACCAGTTACTCCCGTTGCTCCAGTTGCACCAGTGGCACCAGTTACTCCCGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCA  >  NZ_CP034943/2073149‑2073426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: