Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,103,772 T→A 8.7% V4V (GTA→GTT azoJ ← FMN‑dependent NADH‑azoreductase AzoJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,103,7720TA 8.7% 391.6 / 7.9 149V4V (GTA→GTTazoJFMN‑dependent NADH‑azoreductase AzoJ
Reads supporting (aligned to +/- strand):  ref base T (66/67);  new base A (14/2);  total (80/69)
Fisher's exact test for biased strand distribution p-value = 6.32e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

AGGAAGGTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAA  >  NZ_CP034943/2103634‑2103909
                                                                                                                                          |                                                                                                                                          
aggaaggTTCTCTTTATGAAGATCTAATTGAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTAAAattata                                                                                                                                           >  1:2264566/1‑140 (MQ=255)
aggaaggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTGTTTAAACataata                                                                                                                                           >  1:17299/1‑140 (MQ=255)
aggaaggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGGTTTTACAAattata                                                                                                                                           >  2:1122482/1‑140 (MQ=255)
aggaaggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAataata                                                                                                                                           >  1:3832346/1‑140 (MQ=255)
     ggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                      <  1:791048/140‑1 (MQ=255)
     ggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                      <  1:1936672/140‑1 (MQ=255)
     ggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                      <  1:4079137/140‑1 (MQ=255)
     ggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                      <  2:550764/140‑1 (MQ=255)
      gTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTAAAAATAATACTTTAg                                                                                                                                     >  1:4836217/1‑140 (MQ=255)
      gTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTTTATTTAAAAATAAAACTGTac                                                                                                                                     >  1:1371566/1‑139 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCCGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  2:4747288/140‑1 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTTATTTTACAAATAATACTGTata                                                                                                                                    >  2:3795950/1‑138 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTata                                                                                                                                    >  2:3734779/1‑138 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  1:873438/140‑1 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  2:4264289/140‑1 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  1:2978108/140‑1 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  2:3734390/140‑1 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  1:2644488/140‑1 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  1:2793125/140‑1 (MQ=255)
       ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATAAGGTAGa                                                                                                                                    >  2:237629/1‑140 (MQ=255)
       gtctcTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTACTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  2:1958867/139‑1 (MQ=255)
        tctcTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGAc                                                                                                                                   >  2:2370018/1‑140 (MQ=255)
          tctTTATGAAGATCTAATTCAACCCCTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCTCTTGATTTTACAAATAATACTGTAGACAt                                                                                                                                 <  1:1559502/140‑1 (MQ=255)
          tctTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACAt                                                                                                                                 <  1:2226495/140‑1 (MQ=255)
             ttATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTTATTTTACAAATAATACTGTAGACAGTTg                                                                                                                              >  2:1269079/1‑140 (MQ=255)
             ttATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTCAAAAAATACTGTATACAATTg                                                                                                                              >  2:3289420/1‑140 (MQ=255)
                 gAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGGTTTTAAAAATATTAATGTAGAGATGTGTAtt                                                                                                                          >  1:3958923/1‑140 (MQ=255)
                      tCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTTGAAATTTGTTTtcgtcc                                                                                                                    >  2:3648941/1‑140 (MQ=255)
                      tCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATtactcc                                                                                                                    >  2:5020679/1‑140 (MQ=255)
                      tCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGGAGACCTTTGTATtcctcc                                                                                                                    >  2:3072868/1‑140 (MQ=255)
                      tCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATAATTTAGACATTTGTATtactcc                                                                                                                    >  2:2211416/1‑140 (MQ=255)
                      tCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAAAACTGTATACATTTGTATtactcc                                                                                                                    >  2:3258828/1‑140 (MQ=255)
                      tCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGATTTTACAAATAATAATGTAGACAGTTGTATtcctcc                                                                                                                    >  2:4419306/1‑140 (MQ=255)
                               aCCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTATACCTTTGTATTACTCCCGGGAAACt                                                                                                           >  2:673090/1‑140 (MQ=255)
                               aCCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTAAAAATAATAAGTTTGACAGTTTTTTTGCTCCCGGGACACt                                                                                                           >  1:3530477/1‑140 (MQ=255)
                                    ttCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTATTCATTTGTATTACTCCCGGTAAAAtttttt                                                                                                      >  2:2648940/1‑139 (MQ=255)
                                        tcatcaGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                  <  2:1233831/140‑1 (MQ=255)
                                        tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTGGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCTATTTATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                  <  2:4512572/140‑1 (MQ=255)
                                        tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                  <  1:938956/140‑1 (MQ=255)
                                        tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                  <  2:1998091/140‑1 (MQ=255)
                                        tcaGCAGGGTGATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTACTTGAAACGCCTTCTTCGGCTGTACTGTCGATTGATTTTACAAATAATACTGTAGACATTTGTAGTCCTCCCGGTAAAATTTTTATAtt                                                                                                  <  1:513863/140‑1 (MQ=255)
                                          agcagGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTaa                                                                                                <  1:3755962/140‑1 (MQ=255)
                                          agcagGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATAATGTATACATTTTTATTGATACCGGTAAACTTTTTATATTaa                                                                                                >  2:4389967/1‑140 (MQ=255)
                                            cagGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGATTTTACAAATAATACTGTAGACAGTTTGATTACTCCCGGTAAACTTTTGataatagat                                                                                              >  2:3451351/1‑137 (MQ=255)
                                             agGGTTTTTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACtt                                                                                             <  1:3434859/140‑1 (MQ=255)
                                              gggTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTCAAATAAAACTGTAGACAATTGTATTGCTCCCGGTTAACTTTTTATATTAAATTa                                                                                            >  2:4183454/1‑140 (MQ=255)
                                              gggTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATACTGTATACATTTGTATTACTCACTGTAAAAATTTTATATTAACTTa                                                                                            >  2:3899308/1‑140 (MQ=255)
                                              gggTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATAATGTAGTCATTTTTATTACTCCCCGTAAAAATTTTATATTAAATTa                                                                                            >  1:2814663/1‑140 (MQ=255)
                                              gggTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAAAAATACTGTAGACATTTGTATTACTCCCGGGAAAATTTTTATATTAACTTa                                                                                            >  2:4338881/1‑140 (MQ=255)
                                                 ttATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAAGAAAACTGGAGACATTTTTTTTCCTGCCGGTAAACATTTTATATTAACTTaaaa                                                                                         >  1:595407/1‑140 (MQ=255)
                                                 ttATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATAATCTAGTCAATTGTATTCCTCCCGGAAAAATTTTTATATTAACTTACaa                                                                                         >  2:2888554/1‑140 (MQ=255)
                                                 ttATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGGGGTTTTAAAAATAAAACTGTATAAAATTTTATTGCGCCCGGGCAACATTTTTTATTAAATTaaaa                                                                                         >  1:1893301/1‑140 (MQ=255)
                                                  tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                        <  1:4356582/140‑1 (MQ=255)
                                                  tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                        <  1:3268891/140‑1 (MQ=255)
                                                  tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                        <  1:1678230/140‑1 (MQ=255)
                                                    ttttCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTTGTTTTAAAAAAAATAAAGTATACAATTGTATTACTCCCCGGAAACCGTTTATATTTAATTACAAATa                                                                                      >  1:2508437/1‑140 (MQ=255)
                                                         tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTACTGCCCGTAAACTTTTTATATTAACTTAAAAAAAGTATg                                                                                 >  2:4224836/1‑140 (MQ=255)
                                                           tataAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTAAAAAAAATACTGTAGACATTTGTATTAGTCCCGGTCAACTTGTTATATTAACTTTAAAATAGTATGtt                                                                               >  1:3315165/1‑140 (MQ=255)
                                                           tataAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTTTAGACAGTTGTATTGCTCCCGGTAAACTTTTTATATTATCTTACAAATAGTATGtt                                                                               >  2:3200796/1‑140 (MQ=255)
                                                               agcagcTAATAAAGACTCGTAACGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCTCTTGATGTTACAAATAATAATGTAGACATTTGTATTCCTCACTGTAAACATTTTATATTAACTTACAAATAGTATGTTTctt                                                                           <  1:4997218/140‑1 (MQ=255)
                                                               agcagcTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTActt                                                                           <  1:4640591/140‑1 (MQ=255)
                                                                   gcTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                    <  1:4879471/80‑1 (MQ=255)
                                                                    cTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTGTTTCAAAAAATAATGGAGACATTTTTCTTGCGCCCGGGGAACGTTTTATATTTAATTTCAAATATTATGGTAgttttttt                                                                      >  1:1127483/1‑140 (MQ=255)
                                                                    cTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGGTTTTTCAAATTATACTTTATAAAGTTTTATTGCGCCCCGTCAAAATTTTATATTAAAATACAAATAGTATTTTTctttattt                                                                      >  1:1025642/1‑140 (MQ=255)
                                                                          aaGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACg                                                                <  2:1783598/140‑1 (MQ=255)
                                                                            gCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCa                                                              <  2:504530/140‑1 (MQ=255)
                                                                            gCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCa                                                              <  2:829872/140‑1 (MQ=255)
                                                                            gCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGGCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCa                                                              <  2:826259/140‑1 (MQ=255)
                                                                             ccTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACAATTGTATTTCTCCCGGTAAACTTTTTATATTAAATTAAAAATAGTATGTTACTTTCTTTTTTAAGCaa                                                             >  2:1942539/1‑140 (MQ=255)
                                                                              cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTAAAAATAATAATTTATACATTTTTATTGCTCCCCGGAAACTTTTTATATTAAATTACAAATAGTGTGTTAATTTGtttttttatgaaa                                                            >  1:4031824/1‑133 (MQ=255)
                                                                              cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTAAAAGAAAACGGTAGACCTTTTTATTGCTCCCCGGAAAGATTGTATTTTAAATTACAAAAAGTAAGTTAATTTATTTTTTACGCcaa                                                            >  2:1368211/1‑140 (MQ=255)
                                                                              cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTTTATTCCGCCCGGTAAACGTTTTATATTAACTTAAAAAAAGTATGTTACTTTCTTTTTTAAGCaaa                                                            >  2:1209129/1‑140 (MQ=255)
                                                                              cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAAAAATAATGTAGACATTTGTATTCCTCCCGGTAAAATTTTTATATTAAATTACAAATAGTAAGTTACTTTGTTTTTTAAGCaaa                                                            >  2:581605/1‑140 (MQ=255)
                                                                              cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTACAAATAAAACTGTTGACATTTGTATTGCACCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTTGTTTGtttttttagcaca                                                            >  2:4644859/1‑133 (MQ=255)
                                                                                cGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGGAGAAATTTGTGTTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTAGTTTATTTTTGTAGCAAAGt                                                          >  2:4780560/1‑140 (MQ=255)
                                                                                cGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACATTAGACATTTTTATTACCCCCGGTAAACTTTTTATATTAACTTACAAATAATATTGTGAGTTATTTTTTACGCCAAGt                                                          >  2:1639263/1‑140 (MQ=255)
                                                                                cGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATAATGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATTTTACTTTATTTTTTACGCAAAGt                                                          >  2:916202/1‑140 (MQ=255)
                                                                                cGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGATTTTAAAAATGATACTGTGGAAATTTGTATTGCTCACGGTCAACATTTTATATTAACTTACAAATAGTATGTTACTTTGTTTTTTACGCAAAGt                                                          >  2:1462706/1‑140 (MQ=255)
                                                                                 gTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGGGATTTTTTAAAAAAAAAGGTAGACAATTTTATTACTCCCCGGAAACGTTTTTTATTAAGTTAAAAAAAATATGTTAATTTATTTTTTAAGCAAACTc                                                         >  1:4349651/1‑140 (MQ=255)
                                                                                   acaGTTTACTTGAAACGCCTTCTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACGGTAGAGATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATTTTACATTCTTTTTTACGTAAAGTCaa                                                       <  2:1317265/138‑1 (MQ=255)
                                                                                     aGTTTAGTTGAAACGCCTTCTTCGTCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATc                                                     <  2:4811521/140‑1 (MQ=255)
                                                                                         tAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACtt                                                 <  1:4624682/140‑1 (MQ=255)
                                                                                                 aCGCCTTCTTCGGCTGTACGGTCGCTTTGTTTTTAAAATAAAAATATATAAATTTTTATTGCTCACCGGAAAAGTTTTTTAATTACTTATAAAAAGTATTTTAGTTTATTTTTTAATCAAACTAAAACACGTTAtttttt                                         >  1:3222848/1‑138 (MQ=255)
                                                                                                                 tACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTAAGCAAAGTCAATCACTTTATTTTTAAAATGAGAtttttttt                         >  2:3806403/1‑140 (MQ=255)
                                                                                                                 tACGGTCGCTTGATTTTAAAAAAAATACTGTAGACATTTGTATTCCTCACCGTCAAATTTTTATATTAAATTACAAATAGTATGTTACTTTGTTTTTGACGCAAAGTCAATAACTTTATTTTGAATATGAGAtttttttt                         >  2:1597077/1‑140 (MQ=255)
                                                                                                                 tACGGTCGCTTGATTTTAAAAAAAATACTGTAGAAATTTTTTTTGCTCCCGGGCAAAGGTTTTTATTAAATTTCAAAAAGTATGTTTCTTTTTTTTTTAAGCACAGTGAAGCACTTTATTTTTTATATTATAtatttttt                         >  1:5008481/1‑140 (MQ=255)
                                                                                                                       cGCTTTATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCaaaaa                   <  1:2916223/140‑1 (MQ=255)
                                                                                                                       cGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCaaaaa                   <  1:3795950/140‑1 (MQ=255)
                                                                                                                       cGCTTGATTTTACAAATAATACAGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCaaaaa                   <  1:3734779/140‑1 (MQ=255)
                                                                                                                       cGCTTGATTTTACAAAGAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAAGAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCaaaaa                   <  1:241924/140‑1 (MQ=255)
                                                                                                                         cTTGATTTTAAAAATAATAATTTATAAAATTTTATTCCTACCCGTAAAAATTTTATAATAACATACAAATAGTATGTTTCTTTATTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCaaaaaaa                 >  2:1997176/1‑140 (MQ=255)
                                                                                                                             aTTTTAAAAAAAATACTGTAGACACTTTTATTCCTCCCGGTAAACTTTTTATATTAAATTAAAAAAAGTATTTTACTTTATTTTTTACGCAAAGTCAATCAAATTATTTTTAATATTAGATTTTTTTTAAAAAAAAAgaa             >  1:4158809/1‑138 (MQ=255)
                                                                                                                               tttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAg           <  2:3574137/140‑1 (MQ=255)
                                                                                                                               tttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTCTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAACGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAg           <  1:4381470/140‑1 (MQ=255)
                                                                                                                                ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt          <  2:3315165/140‑1 (MQ=255)
                                                                                                                                ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt          <  2:702596/140‑1 (MQ=255)
                                                                                                                                ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt          <  2:3077900/140‑1 (MQ=255)
                                                                                                                                ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt          <  2:4350262/140‑1 (MQ=255)
                                                                                                                                 tACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTa         <  2:392768/140‑1 (MQ=255)
                                                                                                                                   cAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACa       <  1:673090/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AGGAAGGTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAA  >  NZ_CP034943/2103634‑2103909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: