Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,139,461 C→A 14.7% A126S (GCG→TCG)  deoD ← purine‑nucleoside phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,139,4610CA14.7% 856.7 / 39.1 380A126S (GCG→TCG) deoDpurine‑nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (225/85);  new base A (2/67);  total (228/152)
Fisher's exact test for biased strand distribution p-value = 4.94e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 398 total aligned reads displayed.

TAATTTGCAAGCTTTTCGATTTGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGT  >  NZ_CP034943/2139322‑2139598
                                                                                                                                           |                                                                                                                                         
tAATTTGCAAGCTTTTCGATTTGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGgcgc                                                                                                                                           <  2:1319742/140‑1 (MQ=255)
      gCAAGCTTTTCGATTTGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATc                                                                                                                                     >  2:3192599/1‑140 (MQ=255)
        aaGCTTTTCGATTTGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCaa                                                                                                                                   >  2:4141952/1‑140 (MQ=255)
                     tGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGc                                                                                                                      >  1:1025372/1‑140 (MQ=255)
                     tGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGc                                                                                                                      >  1:3446763/1‑140 (MQ=255)
                      gCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCg                                                                                                                     >  2:5197138/1‑140 (MQ=255)
                      gCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCg                                                                                                                     >  2:1082389/1‑140 (MQ=255)
                       cGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGAATCATAGGCATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGa                                                                                                                    <  2:2514195/140‑1 (MQ=255)
                         aaTCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGAct                                                                                                                  <  2:3498291/140‑1 (MQ=255)
                              gggtTGTCGAACTGATCCGGTGTAAATCCGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATACGCATTTTTTAAACGCTCCAAATCTGCGCAAGGCGCAAAAACAATACTTCCGAAAGCGACTCTGtt                                                                                                             <  2:4286396/138‑1 (MQ=255)
                                        atttagcAGGTGTAAATACGCTCCCGCCGGTCACGGGCCCGCCTTTATCCTTGGGGGAATCATAGCCATTTTTTAAAAGCTCGAAAGGTGCGCACGGCGAAAAATCAATCCTGCCCAAAGCGACTCTGTTCATTTGTGaa                                                                                                   <  2:3293761/134‑1 (MQ=255)
                                        aCTTATCAGCTGTAAATACGCTTCCTCCCGACACCGGCACGCATTTATCCTTTGCGGAATCATAGGCATTTTTTAAAAGCTCCAAATCGGCGCACGGCGAAAAATCAATACTCCCCAACGCGACTCTGTTCATTTGTGaa                                                                                                   <  1:2116863/140‑1 (MQ=255)
                                         cTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTGTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                  >  2:4653319/1‑140 (MQ=255)
                                          ttaTCAGCTGTAAATACGCTTCCCCCGGCCCCGGGCACGCCTTTATCCTTTGCGGCATCATAGGAATTTTTAAAAAGCGCCAAAGCGGCGCAAGGCGCAAAATAAATCCTTCCGAACGCGACTCTGTTCATTTGTGAATc                                                                                                 <  2:4214185/138‑1 (MQ=255)
                                          tGATCAGGTGTAAATACGCTGCCTCCCGGCCCCGGCACGCCTTTATCCTTGGCGGCATCATAGGCATTTTTTAAAAGCTCGAAAGCGGCGCAAGGCGAAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATc                                                                                                 <  2:1551496/140‑1 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATAGTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:2694276/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:581602/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:8639/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:3908240/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  2:4541755/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:289079/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:1107034/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  2:1819745/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:2190791/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:2001819/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  2:2756108/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:1394155/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                               >  1:1137639/1‑140 (MQ=255)
                                            aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTATGTTCATTTGTGAATAAg                                                                                               >  2:2249406/1‑140 (MQ=255)
                                                 cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAg                                                                                          >  1:514725/1‑140 (MQ=255)
                                                 cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTGGCGGAATCATCGCCATTTTTTAACACCTCGAAATCTGCGCAAGGCCAAAAATAAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAg                                                                                          <  1:5047952/140‑1 (MQ=255)
                                                 cTGTAAATAAGCTTCCTAACGTCACCGGCACGCCTTTAACCTTTGCGGCATAATAGGCATTTTTTAAAAGCTCGAAAAATGCGCAAGGCGAAAAATCAATAATTCCGAAAGAGACTATGTTCAGTTTTGAATCAGTTGAg                                                                                          >  1:4296808/1‑140 (MQ=255)
                                                  tgaaactACGGTCCCCACCGCCACCGGCCCGCCTTTAGCGTTTGCGGCACCATAGGAATTTTTTAAAAGCTCGAACTCTGCGCAAGGCGAAAAAACAAGACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                         <  1:4814742/134‑1 (MQ=255)
                                                  tGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                         <  2:1137639/140‑1 (MQ=255)
                                                  ggTAAATACGCTTCCTCGCGTCCCGGGCACGCGTTTATCCTTTGCTGAATCATAGGCATTTTTTAATTGATCGAATGATGCTCAAGGCGCAAAATCAATACTGCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                         <  2:2839083/139‑1 (MQ=255)
                                                      aaTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGt                                                                                     >  1:3093874/1‑140 (MQ=255)
                                                         aCGCTTCCTACCGTCACCGGCACGCCTTTATCGTTTGGGGCATAATAGGAATTTTTAAAACGCACGAAATCTGCGCAAGGCGCAAAAACAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCAt                                                                                  <  2:1791720/140‑1 (MQ=255)
                                                         aCGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATCGGCATTTTTTAAAAGCTCGAAATCGGCGCAAGGCGCAAAATCAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCAt                                                                                  <  2:3016959/140‑1 (MQ=255)
                                                         aCGCTTCCAACCGTCACCGGCACGCCTTTATCCTTTGGGGCATCATCGGAATTTTTTAAAAGCACGAAATCTCCGCAAGGCGAAAAATCAATACTTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCAt                                                                                  <  1:4229061/140‑1 (MQ=255)
                                                           taatacTACCGACACCGGAAAGCATTTATCAAATGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                >  1:1504704/6‑140 (MQ=255)
                                                           gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATCGGCATATTTTAAAAGCTCGAAATCTCCGCACGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                >  2:2149635/1‑140 (MQ=255)
                                                           gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                >  2:1679537/1‑140 (MQ=255)
                                                           gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                >  2:141309/1‑140 (MQ=255)
                                                           gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                >  1:3190101/1‑140 (MQ=255)
                                                           gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                >  2:2332589/1‑140 (MQ=255)
                                                              tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGATTCAGTTGAGGATGTCATATCCa                                                                             >  2:2485847/1‑140 (MQ=255)
                                                                  aCCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAAt                                                                         >  1:377770/1‑140 (MQ=255)
                                                                  aCCGTCACCGGCACGCCTGTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATAGTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAAt                                                                         >  1:2435667/1‑140 (MQ=255)
                                                                     gTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGAc                                                                      >  1:658132/1‑140 (MQ=255)
                                                                         ccGGCACGCCTTTATCCTTTGCGGCATCATAGCCATTTTTAAAAAGCTCGAAAGCGGCGCAAGGCGCAAAATCAATACTCCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                  <  2:2084252/140‑1 (MQ=255)
                                                                            gCACGCTTTTATCCTTTGCGGATTAATTGGCTTTTTTTAAAAGCTCGAAATCTGCGCACGGCGCAAAATCACTCCTCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGc                                                               <  1:493233/140‑1 (MQ=255)
                                                                            gCACGCCTTTCTCCTGTGCGCCATCATCGGCATTTTTTAAAAGCTCGAAATCGGCCCACGGCGAAAAATAAATACTTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGc                                                               <  1:4349405/140‑1 (MQ=255)
                                                                            gCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGc                                                               <  2:3684300/140‑1 (MQ=255)
                                                                            ccACGCCTTTATCCTTTGCGGCATAATAGGAATTTTTAAAAAGCTCTAAATCTGCGAAAGGCGAAAAAACAATACTGCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGc                                                               <  1:3384984/139‑1 (MQ=255)
                                                                                     tATCCTTTGCGGCATCATCGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTGCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                      <  2:1457197/140‑1 (MQ=255)
                                                                                     tATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                      >  1:4046975/1‑140 (MQ=255)
                                                                                           ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATAGTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTg                                                >  1:3553678/1‑140 (MQ=255)
                                                                                           ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAACCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGACAAAATGACGTCCCGCACTGTGACATCTTTg                                                >  1:1295594/1‑140 (MQ=255)
                                                                                            ttcGGCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGAGAATCTGTTCATTTGTGAATCAGTTGAGGATGTCATATCCAAAATGACTTCCCGCACTTTGACATCTTTGc                                               >  1:1853578/3‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:4511311/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  2:4839944/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:1791824/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:2531371/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:2639690/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  2:436118/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  2:106328/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:912039/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:3690932/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:3860285/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:3948284/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:4738095/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:4655094/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:4616280/1‑140 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACttt                                                          <  2:2212815/129‑1 (MQ=255)
                                                                                            tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGAGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTTTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  1:788112/1‑140 (MQ=255)
                                                                                            tGCGGCATCATACGCATATTTTAAAAGCGCGAAATATTAGCAAGTCGAAAAATCAAAACTTCCGAAAGAGACTATCATCATTTGTGAATCAGTTGAGGATGTCATAGACAAAATGACGACCCGAACTTTCACATAtttca                                               >  2:2656505/1‑138 (MQ=255)
                                                                                            atcgtcatcatAGGCATTTTTTAAAATCTCCAAATCTGCTCAATTCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                               >  2:2392130/6‑140 (MQ=255)
                                                                                                 catcatAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATa                                          >  1:5095962/1‑140 (MQ=255)
                                                                                                   tcatAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgc                                        >  2:767874/1‑140 (MQ=255)
                                                                                                      tAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGcgcc                                     >  2:717688/1‑140 (MQ=255)
                                                                                                         gaaTTTTTTAAAAGCTCTAAATCTGCGAAAGGCGCAAAATCAATCCTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                  <  2:377770/138‑1 (MQ=255)
                                                                                                         gCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACGTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                  <  2:3560289/140‑1 (MQ=255)
                                                                                                         gCATTTTTTAAAAGATCGAAATCTGCGCAAGGCGAAAAAGCAATACTGCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                  <  2:611982/140‑1 (MQ=255)
                                                                                                         gCATATTTTAAAAGCTCGAACTCTGCGCAAGGCGCAAAATCAATCCTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                  <  2:4976945/140‑1 (MQ=255)
                                                                                                         ccATTTTTTAAAAGCTGTAAATCCGGCAAAGGCCAAAAATCAATAGTTCCCACAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                  <  1:4882684/139‑1 (MQ=255)
                                                                                                             tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGac                              >  1:2979202/1‑139 (MQ=255)
                                                                                                             tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGaa                              >  1:3211263/1‑140 (MQ=255)
                                                                                                                ttAAACGCTCTAATTCGGCGAAAGGCGAAAAATCAATACTCCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCt                           <  1:5197138/140‑1 (MQ=255)
                                                                                                                 tAAACGCTCTAAATCTGCGCAAGGCGCAAAAACAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                          <  2:341543/140‑1 (MQ=255)
                                                                                                                 tAAAAGCTCTAAATGTGCGCAAGGCGAAAAATCAAAACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                          <  2:3933726/140‑1 (MQ=255)
                                                                                                                 tAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                          <  1:106328/140‑1 (MQ=255)
                                                                                                                 tAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                          <  2:2564546/140‑1 (MQ=255)
                                                                                                                         cGAAATCTGCGCAAGGCGAAAAATCAATCCTCCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                  <  1:2055238/140‑1 (MQ=255)
                                                                                                                         cGAAATCTGCGAAAGGCGAAAAATCAATACTGCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                  <  2:1025372/140‑1 (MQ=255)
                                                                                                                                 ggccAAGGCGCAAAACAAATAGTCCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTg          <  2:372284/137‑1 (MQ=255)
                                                                                                                                  cgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGc         >  1:2284992/1‑140 (MQ=255)
                                                                                                                                  cgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGc         >  1:2741960/1‑140 (MQ=255)
                                                                                                                                  cgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGc         >  1:162580/1‑140 (MQ=255)
                                                                                                                                  cgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGc         >  1:3620719/1‑140 (MQ=255)
                                                                                                                                        ccgcAAAATCAATACTCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCg   <  1:4151277/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TAATTTGCAAGCTTTTCGATTTGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGT  >  NZ_CP034943/2139322‑2139598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: