Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,147,508 T→G 6.3% T173T (ACA→ACC EO946_RS10810 ← CoA transferase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,147,5080TG 6.3% 447.0 / 12.4 159T173T (ACA→ACCEO946_RS10810CoA transferase subunit A
Reads supporting (aligned to +/- strand):  ref base T (74/74);  new base G (3/8);  total (77/82)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 163 total aligned reads displayed.

CGCTTCTCACAACCCCCTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCATTTGAT  >  NZ_CP034943/2147369‑2147647
                                                                                                                                           |                                                                                                                                           
  cTTCTCACAACCCCCTCGACAATAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCAGATTGCGCGCTGt                                                                                                                                           >  2:2111291/1‑140 (MQ=255)
  cTTCTCACAACCCCCTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGt                                                                                                                                           >  2:1448685/1‑140 (MQ=255)
  cTTCTCACAACCCCCTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGt                                                                                                                                           >  2:1792640/1‑140 (MQ=255)
  cTTCTCACAACCCCCTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGt                                                                                                                                           >  1:4523914/1‑140 (MQ=255)
  cTTCTCACAACCCCCTCGAAAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGCGACGATCCTCTCAGCTTCGGCAAAGGTTATCCTGGCAGCCATTGCCATAAGCGCAGTCATATTGCGCGCTgg                                                                                                                                           >  2:2111393/1‑139 (MQ=255)
        acaACCCCCTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGtt                                                                                                                                     <  2:3703235/140‑1 (MQ=255)
        acaACCCCCTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGtt                                                                                                                                     <  1:1708909/140‑1 (MQ=255)
             ccccTCGACAAAAACCCCCGGTGTGAGAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACg                                                                                                                                >  2:5081221/1‑140 (MQ=255)
             ccccTCGACAAAAAACCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCGGTTTTGTTATACg                                                                                                                                <  2:54712/140‑1 (MQ=255)
               ccTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGtt                                                                                                                              >  1:3237196/1‑140 (MQ=255)
               ccTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGtt                                                                                                                              >  2:4362033/1‑140 (MQ=255)
               ccTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGtt                                                                                                                              >  2:1908494/1‑140 (MQ=255)
               ccTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGtt                                                                                                                              >  1:4520357/1‑140 (MQ=255)
               ccTCAACAAAAACCCCCGGTGTGACAATCATTTCTTCAGAAATCTCCCCCATCGGAACGATACGCTCAGCTTCGGCAAAGGTTCTCCTGGCGGCCATTGCCATAAGCGGGTTCATATTGCGCGCGGTTTTGtc                                                                                                                                     >  2:3909531/1‑132 (MQ=255)
               ccTCAACAAAAACCCCCGGTGTGACAATCATTTCTTCAGAAATCTCCCCCATCGGAACGATACGCTCAGCTTCGGCAAAGGTTCTCCTGGCGGCCATTGCCATAAGCGGGTTCATATTGCGCGCGGTTTTGtc                                                                                                                                     <  1:1315268/133‑2 (MQ=255)
                cTCGACAAAAACCCCCGGTGTGACATTGTTTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTa                                                                                                                             <  1:4955488/140‑1 (MQ=255)
                cTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTa                                                                                                                             <  1:2156518/140‑1 (MQ=255)
                cTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTa                                                                                                                             >  2:1247582/1‑140 (MQ=255)
                cTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTa                                                                                                                             <  1:997925/140‑1 (MQ=255)
                    aCAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGAGTCATATTGCGCGCTGTTTTGTTATACGTTAAAtt                                                                                                                         >  1:4004063/1‑140 (MQ=255)
                        aaaCCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCg                                                                                                                     >  1:4204659/1‑140 (MQ=255)
                          aCCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGaa                                                                                                                   >  2:3061551/1‑140 (MQ=255)
                          aCCCCCGGTGTGACAATCAGTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGaa                                                                                                                   <  2:2483744/140‑1 (MQ=255)
                              ccGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCa                                                                                                               >  1:4774052/1‑140 (MQ=255)
                              ccGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCa                                                                                                               >  1:3533198/1‑140 (MQ=255)
                                 gtgtGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCg                                                                                                            <  2:4647727/140‑1 (MQ=255)
                                 gtgaGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCg                                                                                                            <  2:5138512/140‑1 (MQ=255)
                                      aCAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGTTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATAGTGCGCGCTGTATTGTTATACGTTAAATTGACGAATTCATCGGCAAt                                                                                                       >  1:5159292/1‑140 (MQ=255)
                                      aCAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAAt                                                                                                       >  1:3626906/1‑140 (MQ=255)
                                            aTTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTgcgc                                                                                                 >  2:2215275/1‑140 (MQ=255)
                                             tttCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCa                                                                                                <  2:4920813/140‑1 (MQ=255)
                                             tttCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCGGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCa                                                                                                <  2:46332/140‑1 (MQ=255)
                                                  tcGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATtgat                                                                                           >  1:3368290/1‑140 (MQ=255)
                                                  tcGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATtgat                                                                                           >  1:3077525/1‑140 (MQ=255)
                                                   cGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATtgatg                                                                                          <  2:1024046/140‑1 (MQ=255)
                                                   cGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCGGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATtgatg                                                                                          <  2:2518128/140‑1 (MQ=255)
                                                   cGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGGTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCGGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATtgatg                                                                                          <  2:3447624/140‑1 (MQ=255)
                                                             tcCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAAt                                                                                <  2:3604774/140‑1 (MQ=255)
                                                             tcCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAAt                                                                                <  2:2996805/140‑1 (MQ=255)
                                                             tcCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAAt                                                                                <  1:3923661/140‑1 (MQ=255)
                                                             tcCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAAt                                                                                <  1:1367759/140‑1 (MQ=255)
                                                             gcccATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATACGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAAt                                                                                <  2:1629777/139‑1 (MQ=255)
                                                                cATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAg                                                                             <  2:4061513/140‑1 (MQ=255)
                                                                         gATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAgcg                                                                    <  1:3444674/140‑1 (MQ=255)
                                                                         gATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAgcg                                                                    >  2:3364694/1‑140 (MQ=255)
                                                                         gATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAgcg                                                                    <  1:2215275/140‑1 (MQ=255)
                                                                               cTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCtt                                                              <  2:82595/140‑1 (MQ=255)
                                                                               cTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCtt                                                              <  2:4774052/140‑1 (MQ=255)
                                                                               cTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCtt                                                              <  2:3533198/140‑1 (MQ=255)
                                                                               cTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCtt                                                              <  2:132876/140‑1 (MQ=255)
                                                                                 cAGCGTCGGCAAAGGTTCTGCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTGTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCa                                                            <  1:5095503/140‑1 (MQ=255)
                                                                                  aGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCaa                                                           <  1:4419875/140‑1 (MQ=255)
                                                                                  aGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCaa                                                           <  1:4686912/140‑1 (MQ=255)
                                                                                                 tctcCTGGCGGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTc                                            <  1:1816690/140‑1 (MQ=255)
                                                                                                 tctcCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTc                                            <  1:3001759/140‑1 (MQ=255)
                                                                                                  ctcCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTcc                                           >  2:940946/1‑140 (MQ=255)
                                                                                                  ctcCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCCAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAAGCAGCCGTCAGCGCCTCTGCAACCAAATACCGCTTTcc                                           >  2:4780291/1‑140 (MQ=255)
                                                                                                     cTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGc                                        <  1:1019173/140‑1 (MQ=255)
                                                                                                        gCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTGTCCAGCcac                                     >  2:2954265/1‑140 (MQ=255)
                                                                                                         cAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCcaca                                    <  1:1935439/140‑1 (MQ=255)
                                                                                                         cAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCcaca                                    <  1:1802422/140‑1 (MQ=255)
                                                                                                         cAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCcaca                                    <  1:1337681/140‑1 (MQ=255)
                                                                                                         cAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCcaca                                    <  1:415263/140‑1 (MQ=255)
                                                                                                          aGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAg                                   <  1:3891690/140‑1 (MQ=255)
                                                                                                               ttGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACg                              <  1:3061551/140‑1 (MQ=255)
                                                                                                               ttGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACg                              <  1:3141515/140‑1 (MQ=255)
                                                                                                               ttGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACg                              <  1:3529994/140‑1 (MQ=255)
                                                                                                                    aTAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTc                         <  1:2496570/140‑1 (MQ=255)
                                                                                                                      aaGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCATATACCGCTTTCCAGCCACAGACACGATGTCCt                       >  1:1626269/1‑140 (MQ=255)
                                                                                                                      aaGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCt                       >  2:4872905/1‑140 (MQ=255)
                                                                                                                      aaGCGGAGTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATGTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTGTCCAGCCCCAGACACGATGTCCt                       >  1:579363/1‑140 (MQ=255)
                                                                                                                            cttCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCt                 >  1:1491991/2‑140 (MQ=255)
                                                                                                                            aTTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCt                 >  2:543133/1‑140 (MQ=255)
                                                                                                                            aTTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCt                 >  1:3311067/1‑140 (MQ=255)
                                                                                                                            aTTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCt                 >  1:724397/1‑140 (MQ=255)
                                                                                                                            aTTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCt                 <  2:1564789/140‑1 (MQ=255)
                                                                                                                            aTTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCt                 >  2:1835386/1‑140 (MQ=255)
                                                                                                                            aTTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAAGTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGAGGTCCTTTTCCt                 >  1:3950717/1‑140 (MQ=255)
                                                                                                                              tCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTca               <  2:5116102/140‑1 (MQ=255)
                                                                                                                              tCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTca               <  2:1644837/140‑1 (MQ=255)
                                                                                                                              tCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTca               <  2:1332464/140‑1 (MQ=255)
                                                                                                                                atatTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTcaca             <  1:3123760/140‑1 (MQ=255)
                                                                                                                                atatTGCGCGCTGGTTTGTTATACGATAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTcaca             <  2:2065832/140‑1 (MQ=255)
                                                                                                                                 tatTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACaa            >  1:5142471/1‑140 (MQ=255)
                                                                                                                                  atTGCGCGCTGTTTTGTTCTACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTcacaga           >  1:897598/1‑138 (MQ=255)
                                                                                                                                    tGCGCGCTGTTGTGTTATAAGTTAAATTGCCGAATTCATCGTAAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGAAACGATGTCCTTTTCCTCACAAAcc         <  1:4264147/140‑1 (MQ=255)
                                                                                                                                      cgcgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCAt       >  1:1741396/1‑140 (MQ=255)
                                                                                                                                      cgcgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCAt       >  1:1523489/1‑140 (MQ=255)
                                                                                                                                      cgcgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCAt       >  2:4679559/1‑140 (MQ=255)
                                                                                                                                      cgcgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACAAAATACCGCTTTCAAGCCACAGACACGATGTCCTTTTCCTCACAAACCAt       >  1:1037317/1‑140 (MQ=255)
                                                                                                                                      cgagatGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCAt       <  2:3379913/135‑1 (MQ=255)
                                                                                                                                       gcgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCTCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCAtt      >  2:3219675/1‑140 (MQ=255)
                                                                                                                                        cgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAg                                                                             >  1:1859215/1‑68 (MQ=255)
                                                                                                                                        cgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCAttt     >  2:3205185/1‑140 (MQ=255)
                                                                                                                                        cgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCAt       >  1:3737730/1‑138 (MQ=255)
                                                                                                                                        cgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGAGGTCCTTTTACTCACAAACCAatt     >  1:2115215/1‑140 (MQ=255)
                                                                                                                                        cgcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTCATGAAAGCAAATTCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTACAGCCACAGACACGATGTCCTTTTCCTCACACACCAttt     >  1:2754110/1‑140 (MQ=255)
                                                                                                                                         gcTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCATTTg    <  1:3908186/140‑1 (MQ=255)
                                                                                                                                          cTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCATTTGa   >  1:1963349/1‑140 (MQ=255)
                                                                                                                                           tGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCATTTGAt  >  2:1871516/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGCTTCTCACAACCCCCTCGACAAAAACCCCCGGTGTGACAATCATTTCTTCGGAAATCTCTCCCATCGGGACGATCCGCTCAGCTTCGGCAAAGGTTCTCCTGGCAGCCATTGCCATAAGCGGATTCATATTGCGCGCTGTTTTGTTATACGTTAAATTGCCGAATTCATCGGCAATTTGCGCATTGATGAAAGCAAAATCAGCCGTCAGCGCCTCTTCAACCAAATACCGCTTTCCAGCCACAGACACGATGTCCTTTTCCTCACAAACCATTTGAT  >  NZ_CP034943/2147369‑2147647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: