Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,491,058 T→G 6.2% E271D (GAA→GAC floA ← flotillin‑like protein FloA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,491,0580TG 6.2% 679.3 / 7.0 271E271D (GAA→GACfloAflotillin‑like protein FloA
Reads supporting (aligned to +/- strand):  ref base T (164/79);  new base G (3/25);  total (167/104)
Fisher's exact test for biased strand distribution p-value = 5.82e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

CGAATGAATCACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCAATGT  >  NZ_CP034943/2490919‑2491194
                                                                                                                                           |                                                                                                                                        
         cACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTGACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTAcg                                                                                                                                 <  2:828752/140‑1 (MQ=255)
                ttCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGgcgc                                                                                                                          >  1:1528714/1‑140 (MQ=255)
                  cTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGgcgcgc                                                                                                                        <  2:1995291/140‑1 (MQ=255)
                     tgtCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCagt                                                                                                                     >  1:3445585/1‑138 (MQ=255)
                     tgtCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCAtt                                                                                                                     >  1:4446816/1‑140 (MQ=255)
                      gtCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGAGCGCATTTCCTCTACGCGGGCGCGCAgtt                                                                                                                    >  2:3062771/1‑140 (MQ=255)
                       tCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCGGCCCGCACAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTCCGCGGGCGCGCATTTc                                                                                                                   <  2:2733200/140‑1 (MQ=255)
                        cAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTcc                                                                                                                  <  2:4234091/140‑1 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCACGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                >  1:2795739/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                >  2:3421685/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                >  2:1754675/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                >  2:4142189/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                >  2:5181357/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                >  1:2837244/1‑140 (MQ=255)
                                     cTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTc                                                                                                                   <  2:1106841/126‑1 (MQ=255)
                                     cTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGa                                                                                                     <  1:3959059/140‑1 (MQ=255)
                                     cTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGa                                                                                                     >  1:4777848/1‑140 (MQ=255)
                                     cTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGa                                                                                                     >  1:1243115/1‑140 (MQ=255)
                                      ttGTTATTCATGTAGTCCATGACGGCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCGGCTTCGGCTTCTACAACTTTCGCGCGCATTACGTCTACGCGGGCGCGCATTTCCTCTTCATGAGCGAc                                                                                                    >  2:2622325/1‑140 (MQ=255)
                                      ttGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGAc                                                                                                    <  1:2207222/140‑1 (MQ=255)
                                      ttGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAGTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGAc                                                                                                    <  1:3979516/140‑1 (MQ=255)
                                        gATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAg                                                                                                  >  2:3972813/1‑140 (MQ=255)
                                        gATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAg                                                                                                  >  1:4277030/1‑140 (MQ=255)
                                         atatTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGc                                                                                                 <  1:4567395/140‑1 (MQ=255)
                                         atatTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGc                                                                                                 <  1:4146758/140‑1 (MQ=255)
                                             tCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGACATc                                                                                             >  1:4485629/1‑140 (MQ=255)
                                             tCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAGTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATc                                                                                             >  1:143902/1‑140 (MQ=255)
                                                tGTAGTCCATGCCGCCAAGAGTCCCTTCCCGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGATTCTACCACTATCGCGCGCCTGTCCTCTACGCGGGCGCGCATGTCCTGTTCATGAGCGACAGCCATCGCa                                                                                          <  1:3401699/140‑1 (MQ=255)
                                                tGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTc                                                                                                                   >  1:526346/1‑115 (MQ=255)
                                                    gTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtc                                                                                      >  2:1533817/1‑140 (MQ=255)
                                                     tCCATGACGCCACTATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCGTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtcg                                                                                     >  1:5129602/1‑140 (MQ=255)
                                                      ccATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCCCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtcgt                                                                                    >  1:3721342/1‑140 (MQ=255)
                                                       cATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAATCGGCACTTCCGCTTCGGCGTCTAGCACTTTAGCGCGGCTTTCCTCTACTCGGGCGCGCATTTCGTGGTCGTGAGCGACGGCCATCGCAcgtcgtg                                                                                   >  2:1656749/1‑139 (MQ=255)
                                                       cATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGtt                                                                                   >  2:1608791/1‑140 (MQ=255)
                                                       cATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGtt                                                                                   >  1:4463480/1‑140 (MQ=255)
                                                        aTGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTc                                                                                                                   >  1:1579657/1‑107 (MQ=255)
                                                          gACGCCAATCTTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGTCTTCTACCACTTTCGCGCGCATGTCCTCGACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                <  1:3760064/140‑1 (MQ=255)
                                                          gACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                <  1:779367/140‑1 (MQ=255)
                                                          gACGCCAATAGTCCCGTCACGCACAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTGGAGCGACAGCCATCGCACGTCGttctt                                                                                <  2:2799556/140‑1 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCTATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  2:3865674/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  1:1665608/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  1:1783375/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  2:1478853/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  2:3404993/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  1:3850626/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  1:3645158/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  1:5043739/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACATTCGCGCGCATTTCCTCTACGCGGGCGCGCAATTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  2:2161334/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCGTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                           >  2:2965064/1‑140 (MQ=255)
                                                                       ccTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTg                                                                   >  1:9948/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCCTTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                 >  1:2435581/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                 >  1:389013/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                 >  2:613786/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                 >  2:650956/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                 >  2:4786345/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                 >  2:3622101/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATAGCAAGCGGCACTTCCGCTACGGCTTCTACCAATTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGATCTTCCGCGTGTGCCTgcg                                                                 >  2:5116094/1‑140 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCCCTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                               <  2:4091949/140‑1 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                               <  1:2355437/140‑1 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                               >  1:4962509/1‑140 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                               <  2:1140422/140‑1 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTACGCTTAGGCTTCTACCACTTTCGCGCGCTTTTCCTCGACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                               <  1:110507/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  1:653961/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  1:578020/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  2:4463480/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  1:3614743/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  1:3243751/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  1:1203465/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAGTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  1:1850958/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCGTCTACCACTTGCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGGCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                     <  2:2836814/140‑1 (MQ=255)
                                                                                     ttCTGACATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATTTTTTTAt                                                     <  2:2060810/140‑1 (MQ=255)
                                                                                       cTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCa                                                   >  1:4006713/1‑140 (MQ=255)
                                                                                        tGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAg                                                  >  2:1813999/1‑140 (MQ=255)
                                                                                        tGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTGTTGCCTGCGCGATGTGTTTATCAg                                                  >  2:2457879/1‑140 (MQ=255)
                                                                                          ccATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCCCTTTCGCGCGCAGTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGcc                                                <  2:2015725/140‑1 (MQ=255)
                                                                                          ccATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGcc                                                <  2:4006713/140‑1 (MQ=255)
                                                                                          ccATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGcc                                                <  2:4159559/140‑1 (MQ=255)
                                                                                          ccATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGcc                                                <  1:4976893/140‑1 (MQ=255)
                                                                                              cGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCttt                                                                         >  2:900240/1‑111 (MQ=255)
                                                                                              cGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCgg                                            >  2:1878427/1‑140 (MQ=255)
                                                                                              cGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCgg                                            >  2:4940197/1‑140 (MQ=255)
                                                                                                  aGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTg                                        >  1:1031212/1‑140 (MQ=255)
                                                                                                    cGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGt                                                           >  1:2009666/1‑119 (MQ=255)
                                                                                                      gCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCg                                    <  1:1754675/140‑1 (MQ=255)
                                                                                                        acgtCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGt                                  <  2:3171939/137‑1 (MQ=255)
                                                                                                        aCTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTTTCAGCCTCGGCCTGATCGGt                                  >  2:2222536/1‑140 (MQ=255)
                                                                                                             cGCTACGGCTACTACCAATTTCGCGCGCATTTCCTCTACGCGGGCGCTCATTTCCTGTTCTTGAGCTACAGCCATCGCACGTCGTTCTTCCCCATTTGCCTGCGCGATGTTTTTATCAGCCTCCGCCTGATCGGATTGTa                             >  2:3462235/1‑140 (MQ=255)
                                                                                                                ttCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTaaaa                          <  1:4109023/140‑1 (MQ=255)
                                                                                                                  cGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATc                        >  1:3066011/1‑140 (MQ=255)
                                                                                                                    gCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGc                      >  1:4378818/1‑140 (MQ=255)
                                                                                                                    gCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGc                      >  2:4360834/1‑140 (MQ=255)
                                                                                                                    gCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGc                      >  2:2925606/1‑140 (MQ=255)
                                                                                                                    gCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGc                      >  2:3291437/1‑140 (MQ=255)
                                                                                                                        cTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCg                  <  1:613786/140‑1 (MQ=255)
                                                                                                                        cTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCg                  >  1:1041869/1‑140 (MQ=255)
                                                                                                                              cTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGttt            >  2:1389945/1‑140 (MQ=255)
                                                                                                                              cTTTCGCGCGCAGGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGttt            <  1:2925606/140‑1 (MQ=255)
                                                                                                                               tttCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGtttt           >  1:5142617/1‑140 (MQ=255)
                                                                                                                                 tCGCGCGCATGTCCTCTCCGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTg         <  1:3421685/140‑1 (MQ=255)
                                                                                                                                        cATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTGTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCAATGt  <  2:5043739/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CGAATGAATCACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCAATGT  >  NZ_CP034943/2490919‑2491194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: