BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,207	G→T	11.2%	Q109K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,207	0	G	T	11.2%	1114.4 / 23.0	515	Q109K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (194/240); new base T (77/3); total (271/244)
Fisher's exact test for biased strand distribution p-value = 2.83e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 515 total aligned reads displayed.

TGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCG  >  NZ_CP034943/240069‑240343

 gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAAGTTTTTTGAGATTTTaa                                                                                                                                        >  1:220454/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCCGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGTATTGCttttg                                                                                                                               >  1:2227463/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCtttac                                                                                                                               >  2:1006653/1‑138 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGAGTATACGGGCGTGTTAGGATTTTTTTTTGAATTTTTGGGAGTTTTGTATGGGttttg                                                                                                                               >  1:4974279/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGCTTTTc                                                                                                                               >  2:1589601/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAATTTTTGGAGATTTTAATTTCtttta                                                                                                                               >  1:1881257/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTc                                                                                                                               >  2:3740598/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                               >  2:2369795/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAAGTTTTTGGAGATTTTAATGGCtttta                                                                                                                               >  1:1961711/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGGTGTCCGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGCTTTTc                                                                                                                               >  1:4912131/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGCTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGTTTTGAATGGCTTTTc                                                                                                                               >  2:4964265/1‑140 (MQ=255)

            agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCg                                                                                                                             >  2:5079192/1‑140 (MQ=255)

            agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCg                                                                                                                             >  2:1508316/1‑140 (MQ=255)

            agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAGTTGAATGGCTTTTCCg                                                                                                                             >  2:4064596/1‑140 (MQ=255)

            agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAATTTTTGGAGATTTGAATTGCttttgag                                                                                                                             >  1:4681495/1‑137 (MQ=255)

                ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTGTAGTTTTTGGAGATTTTAATTGCTTTTGCGtttt                                                                                                                         >  1:1532657/1‑140 (MQ=255)

                ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTATTTTTGGGAGATTTGAATTGCTTTTACGtttt                                                                                                                         >  1:2814001/1‑140 (MQ=255)

                    cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTGTTCCGTTTTTaaa                                                                                                                     >  2:670810/1‑140 (MQ=255)

                    cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTaaa                                                                                                                     >  2:4889027/1‑140 (MQ=255)

                    cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTaaa                                                                                                                     >  2:4135932/1‑140 (MQ=255)

                    cATCTCTGATGTTGGAATTATTCAGAGATGCTGGTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTtaa                                                                                                                     >  2:2894154/1‑140 (MQ=255)

                      tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTGGGAGATTTTAATTGCTTTTACGTTTTTaaaaa                                                                                                                   >  1:3484779/1‑139 (MQ=255)

                      tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                                   >  1:1057839/1‑140 (MQ=255)

                      tctcTGATGTTGGAATTATTCAGAGATGATGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTGCGTTTTTAAAAc                                                                                                                   >  1:2867799/1‑140 (MQ=255)

                       gtctGATGTTGGAATTATTCAGTGATTCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGTATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                  <  1:4376752/139‑1 (MQ=255)

                           gATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAagag                                                                                                              >  2:3160579/1‑140 (MQ=255)

                             tGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGc                                                                                                            <  2:4429914/140‑1 (MQ=255)

                                tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                         <  2:2407893/140‑1 (MQ=255)

                                tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                         <  1:2294672/140‑1 (MQ=255)

                                tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGGTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                         <  1:4062245/140‑1 (MQ=255)

                                tGGAATGATTCAGAGATGCTGTTTTTGTATTTTGGTTCTCAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                         <  1:3070501/140‑1 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTGAATTGGTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                        >  1:1770815/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGTGAGCTTTTTATTGCGTTTCAGTTTTTTAAAACAGAGGCAAGc                                                                                                        >  1:3958496/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATTGCTTTTCAGTTTTTAAAACAAGAGGCAAGc                                                                                                        >  2:4713316/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                        >  2:280252/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                        <  1:950921/140‑1 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                        >  2:580454/1‑140 (MQ=255)

                                      tattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGAt                                                                                                   <  2:2608854/140‑1 (MQ=255)

                                       attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                  <  2:5040338/140‑1 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAAAAATAGGGAATCCAGATAAACCCg                                                                                            >  1:1667585/1‑140 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCg                                                                                            >  1:3614448/1‑140 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAAACCg                                                                                            >  2:1873334/1‑140 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAATTTTTTGGAGATTTTTATTGCTTTTCCGTTTTTAAAACAAGAGGCAACCCACAAAAAcccc                                                                                            >  1:795981/1‑139 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTGAAGTTTTGGGAGATTTGAAAGTCGTTTGCGTTTTTTAAACAAGATGCAAGCCCGAAAAAcccc                                                                                            >  1:203796/1‑139 (MQ=255)

                                                tGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGATGCAAACCAGATAAACCCGaca                                                                                         >  2:4474615/1‑140 (MQ=255)

                                                tGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGCTTTTACGTTTTTAAAACAAGAGGCAATCCATATAAACACGaca                                                                                         >  2:1266154/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTTCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCACATAAACCCGacaac                                                                                       >  2:3473134/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGAAAGCCAGATAAAACCGacaac                                                                                       >  2:2558010/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGATATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                       >  2:4302425/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTAAAAAAAGAGGCAAGCCACATAAACCCGacaac                                                                                       >  2:2020950/1‑140 (MQ=255)

                                                       tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTTAGTTTTTGGGGATTTTAATTGCGTTTGCGTTTTTAAAAAAATAGGGAACCCAGATAAACCCCAaaacaacaa                                                                                  >  1:1352302/1‑140 (MQ=255)

                                                             tttttGTTGTCATACGAAGTCGATCATTCCGCCTCGCCGGGCGTTTTCGGATTTTTGTGTGAAGTTTTGGGAGATTAGAAGTGCTTTTACGTTTGTAATTAAAGCGGGAAGCCCGAAAAACCCGCCCCCAACcaaaaagt                                                                            >  2:4238286/1‑138 (MQ=255)

                                                               tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                          <  1:3221357/140‑1 (MQ=255)

                                                               tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGGTCGGGTTTTTTTTTGTAGTTTTGGGTGATTTGTATGGCTTTTGCGTTTTTAAAAAAAGTGGCAAGCCAGATAAAACCGCCAACAACAAAAaataaa                                                                          >  1:1466007/1‑139 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                      <  1:3831333/140‑1 (MQ=255)

                                                                   tgcTAATACGAAGTCGCTCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                      <  2:2232599/138‑1 (MQ=255)

                                                                        aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                 <  2:3082177/140‑1 (MQ=255)

                                                                        aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                 <  2:1412838/140‑1 (MQ=255)

                                                                        aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                 <  2:3376429/140‑1 (MQ=255)

                                                                         tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                <  1:2950620/140‑1 (MQ=255)

                                                                         tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                <  1:4185101/140‑1 (MQ=255)

                                                                         tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAATTTTGTTTGAAGTTTTGCGAGTTTTGAATGGCTTTTCCGTTTTTAAAACATGAGGCAAGCCAGATAAAGCGGACTACAACAATAAATAATCTTGCAAGTa                                                                <  1:5115105/140‑1 (MQ=255)

                                                                         tACGAAGTCGATCATTCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATGTGAATGGCCTTTCCGTTATTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                <  2:3856058/140‑1 (MQ=255)

                                                                            gAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAAc                                                             <  2:1355144/140‑1 (MQ=255)

                                                                             aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGAGTTTAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACt                                                            >  2:927550/1‑140 (MQ=255)

                                                                             aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACt                                                            >  2:5178336/1‑140 (MQ=255)

                                                                              aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                           <  1:3294828/140‑1 (MQ=255)

                                                                              aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                           <  1:5038202/140‑1 (MQ=255)

                                                                                   aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGCTTTGAATGGCTTTTCCGTTTTTAAAACCAGAGGCAAGCCAGATAAAACCGACAACAACAAAAAAAAATATAGCAAGTAAAATCcaatc                                                      >  2:1723651/1‑137 (MQ=255)

                                                                                   aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAAAAAAAAATAAACTAGCAAGTAAACTCcatgc                                                      >  2:3209917/1‑140 (MQ=255)

                                                                                    tCATTCCTACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTGTGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACAAAAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:1600342/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCACGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  2:114240/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:913718/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:1140024/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:4828942/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:2379524/140‑1 (MQ=255)

                                                                                        tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                                                 <  2:851270/140‑1 (MQ=255)

                                                                                         acGACTATACGGGAGTTTTCGGATTTTTGTTTGAAGTTTTGGGATATTTTAATGTCTTTTCAGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATgggg                                                <  1:64827/139‑1 (MQ=255)

                                                                                                  cGGGCGTTTTCGGATTTTTGTTTTAAATTTTGGGGGAGTTGAATTGCTTTTGCGTTTTTAAAACAAGAGGCAAGCCCCATAAACCCCCCAACAAAAAAAAAAAAACTATCAAGTAAACTACAAGCATGGGGTaattaatt                                       >  1:4561786/1‑140 (MQ=255)

                                                                                                  cGGGCGTGTTGGGATTTTTGTTTTTAGTTTTTGGGGATTTGAATTGCTTTTCCGGTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACAAAAAAAAATCTAGCAAGTAAAATCCATGCATTGGGTAattcctg                                       >  1:1185481/1‑137 (MQ=255)

                                                                                                    ggCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCg                                     <  2:3928265/140‑1 (MQ=255)

                                                                                                        ttttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCAGTCGTTGc                                 <  2:4836344/140‑1 (MQ=255)

                                                                                                          ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAATGGTTTTGCGTTTTTAAAACAAGAGGCCACCCAGATAAAACCGGCACCAAAAAAAAATAATCTATCACGTAAACTCCATGCATGGGGTGATTCATTGGTTGaaa                               >  2:2062287/1‑140 (MQ=255)

                                                                                                                 ttttGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaa                        <  2:2131963/140‑1 (MQ=255)

                                                                                                                    tgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa                     <  2:4644158/140‑1 (MQ=255)

                                                                                                                    tgtctgAAGTTTTGGGAGATTTGCATGGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa                     <  1:3153383/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGCTTTGAATCGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                    <  1:2754385/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                    <  1:1294788/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                    <  2:211158/140‑1 (MQ=255)

                                                                                                                        tgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAATTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATaga                 <  2:1814222/140‑1 (MQ=255)

                                                                                                                               ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATTCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa          <  2:3257564/140‑1 (MQ=255)

                                                                                                                               ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa          <  2:3603408/140‑1 (MQ=255)

                                                                                                                               ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa          <  2:1213068/140‑1 (MQ=255)

                                                                                                                                tGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa          <  2:739502/139‑1 (MQ=255)

                                                                                                                                  ggAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAtg       <  2:2733005/140‑1 (MQ=255)

                                                                                                                                      aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAACATAGAGTTACTAATGTGcc   <  1:532173/140‑1 (MQ=255)

                                                                                                                                      aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc   <  2:4156615/140‑1 (MQ=255)

                                                                                                                                      aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc   <  1:258755/140‑1 (MQ=255)

                                                                                                                                      aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc   <  1:716364/140‑1 (MQ=255)

                                                                                                                                      aTTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAGACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc   <  1:1834677/140‑1 (MQ=255)

TGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCG  >  NZ_CP034943/240069‑240343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`