Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,627,197 G→T 6.1% intergenic (‑18/‑115) EO946_RS13595 ← / → EO946_RS13600 YrzK family protein/SH3 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,627,1970GT 6.1% 727.9 / 12.0 256intergenic (‑18/‑115)EO946_RS13595/EO946_RS13600YrzK family protein/SH3 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (78/156);  new base T (20/2);  total (98/158)
Fisher's exact test for biased strand distribution p-value = 1.32e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GACCAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGAAATACTTTGTTC  >  NZ_CP034943/2627059‑2627333
                                                                                                                                          |                                                                                                                                        
gACCAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGa                                                                                                                                         <  2:969175/140‑1 (MQ=255)
 aCCAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGAt                                                                                                                                        <  2:4440045/140‑1 (MQ=255)
 aCCAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGAt                                                                                                                                        <  2:4128746/140‑1 (MQ=255)
 aCCAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGAt                                                                                                                                        <  2:2508082/140‑1 (MQ=255)
  ccAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGAtt                                                                                                                                       <  2:3434020/140‑1 (MQ=255)
  ccAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGAtt                                                                                                                                       <  2:2622062/140‑1 (MQ=255)
   cAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTg                                                                                                                                      <  2:3500648/140‑1 (MQ=255)
   cAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTg                                                                                                                                      <  1:699262/140‑1 (MQ=255)
   cAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTg                                                                                                                                      <  1:2613765/140‑1 (MQ=255)
     aaTTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGTTATTTCATGATTTGCTCACCGTTTTGATTGTa                                                                                                                                    >  1:4875120/1‑140 (MQ=255)
     aaTTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCAACGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTa                                                                                                                                    >  2:1947802/1‑140 (MQ=255)
      aTTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTTCTTAAACGTGGTGAAATGCACGTCCCAATTTTTGATGATATTTAATGATTTTCAAAACGTTTTGATTTTAt                                                                                                                                   >  1:3476756/1‑140 (MQ=255)
       ttCAGATGCTGTTTCAGTAAGAGAACCGGTCGTTTCGGGAGCTGCGTGATCGCTGTAATGCAGATTTGCTTCCCCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTAtt                                                                                                                                  <  1:4269210/140‑1 (MQ=255)
       ttCAGATGATGGTTCAGTAAGATAACCGGTCGTTTCGGTATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTAGTGAAAGGAACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTAtt                                                                                                                                  <  1:481937/140‑1 (MQ=255)
            ttGCTGTTTCAGTAAGATAACCGGTAGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGc                                                                                                                             <  2:2100985/139‑1 (MQ=255)
            ctGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGc                                                                                                                             <  1:3970231/139‑1 (MQ=255)
            ctGCTGTGTCAGTAAGATAACCGGTCGTGTCGGGATCGGCGTGATCGCTGTACTGCAGATTTGCGTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGc                                                                                                                             <  1:3324823/139‑1 (MQ=255)
            aggCTGTTTCAGGAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGc                                                                                                                             <  2:4362436/138‑1 (MQ=255)
            aTGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGc                                                                                                                             <  1:1069928/140‑1 (MQ=255)
            aTGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGc                                                                                                                             <  2:2608787/140‑1 (MQ=255)
             tGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGcc                                                                                                                            <  2:1045005/140‑1 (MQ=255)
             tGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGACATTTCATGATTTGCACACCTTTTTGATTGTATTTATGcc                                                                                                                            <  1:1524949/140‑1 (MQ=255)
             tGCTGTTTCAGGAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGcc                                                                                                                            <  1:1414661/140‑1 (MQ=255)
                tGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGTAATGCACGTGCCATTTTTGGATGATATTTCATGATTTGCAACCCTTTTTGAATGTATTTATGCCTAg                                                                                                                         >  2:2074767/1‑140 (MQ=255)
                 gTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGt                                                                                                                        >  2:3588831/1‑140 (MQ=255)
                 gTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGt                                                                                                                        >  2:4326539/1‑140 (MQ=255)
                 gTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCCTCGTGAAATGCACGTCCCATTTTTGGATTATATTTCATGATTTTCAAACCTTTTTTATTGTATTTATGCCTAGt                                                                                                                        >  2:632420/1‑140 (MQ=255)
                 gTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATAGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCAAACCTTTTTGATTGTATTTATGCCTAGt                                                                                                                        >  2:2826210/1‑140 (MQ=255)
                 gTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATAGCTGTAAAGCAGATTTGCTTCACCGTCGTGAAATTCACGTACCATAATTGGATCAAATTTCAACATTTGCACACCGTTTTGATTATATTTATGACAAGt                                                                                                                        >  2:4497073/1‑140 (MQ=255)
                  tttCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATTAAGATTTTCTTCACCGTCGTTAAATGCACGTCCCATTTTTTGATGATATTTAATGATTTTAAAAACTTTTTGATTGTATTTATGCCTATta                                                                                                                       >  2:4219948/1‑140 (MQ=255)
                  tttCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTTATTGTATTTATGCCTAGta                                                                                                                       >  2:3325455/1‑140 (MQ=255)
                  tttCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGta                                                                                                                       >  2:2698310/1‑140 (MQ=255)
                  tttCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACAGTCGTGAAATGCACGTCCCATTTTTTGATGATATTTCATGATTTGCACACATTTTTTATTGTATTTATGCCTAGta                                                                                                                       >  2:2998180/1‑140 (MQ=255)
                          aGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCAACGTCGTGAAATTCAAGTCCCATTTTTGGATGATATTTCATGATTTGCACACCATTTTTATTTTATTTATTCCTAGTATAAACaaa                                                                                                               >  1:4056210/1‑140 (MQ=255)
                           gATAACCGGTCGTTTCGGGCTCTGCGTGATCGCTGTAATGCAGATTTGCGTCACCGTCGTGAAATTCACGTGCCATTTTTTGATGATATTTAAAGATTTGCCCACCATTTTGATTGTATTTATTCCTAGTATAAACaaaa                                                                                                              >  1:3379701/1‑139 (MQ=255)
                           gATAACCGGTCGTTTCGGGATCTGCGTGATAGCTTTAATTCAGATTTTCTTAAACGTCGTTAAAAGCACCTCCCAATTTTGGATGATATTTAATTATTTTGACACCTTTTTTTTTTTATTTATTCCTAGTAaaaaaaaaa                                                                                                              >  1:3687381/1‑139 (MQ=255)
                            aTAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATc                                                                                                             >  2:1101462/1‑140 (MQ=255)
                            aTAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATAATTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATc                                                                                                             >  1:3420643/1‑140 (MQ=255)
                            aTAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAAAGCAGATTTGCTTCACCCTCGTGAAATGCACGTCCCCTTTTTGGATGATAATTCATGATTTGCACACCTTTTTGATTGTATTTTTTCCTAGTATAAACAAATc                                                                                                             >  2:1295610/1‑140 (MQ=255)
                            aTAACCGGTCGTTTCGGGATCTGCGTGATAGCTGTAATGCAGATTTGCTTAACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGGACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATc                                                                                                             >  1:4381481/1‑140 (MQ=255)
                                ccGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTTCTTCACCGTCGTGAAATGCACCTCCCAATTTTGGATGATATTTAATGATTTTCACACCATTTTTATTTTATTTATGCCTAGTATAAAAAAATCattt                                                                                                         >  1:4082295/1‑140 (MQ=255)
                                ccGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGc                                                                                                                             >  2:4082223/1‑120 (MQ=255)
                                ccGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCCTCGTGAAATTCACGTCCCATTTTTTGGTGATATTTAATGATTTGCACACCTTTTTTATTTTATTTATTCCTAGTATAAACAAATCattt                                                                                                         >  1:4570909/1‑140 (MQ=255)
                                ccGGTCGTTTCGGGATCTGCGTGATAGCTGTAAAGCAGAATTGCTTCACCCTCGTGAAAATCACCTCCCATTTTTTGATGATATTTAATTATTTTCAAAACATTTTTATTTTATTTATTACTAGTATAAAAAAAtaaatt                                                                                                         >  1:4138077/1‑135 (MQ=255)
                                      gTTTCGGGATCTGCGTGATCGCTGTAATGCCCATTTTCTTAACCGTCGTGAAATTCACGTCCCATTTTTGGATGATATTTAAAGATTTTCAAACCTTTTTTATTGTATTTTTGGCTAGTATAAAAAAATAattttatttt                                                                                                   >  1:2897834/1‑140 (MQ=255)
                                                cTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTaaaaa                                                                                         <  2:2411551/140‑1 (MQ=255)
                                                cTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTaaaaa                                                                                         <  2:670376/140‑1 (MQ=255)
                                                 tGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAAt                                                                                        >  1:3648156/1‑140 (MQ=255)
                                                     tGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGa                                                                                    <  2:3476756/140‑1 (MQ=255)
                                                       aTCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGaaa                                                                                  >  1:4727249/1‑140 (MQ=255)
                                                       aTCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGaaa                                                                                  >  1:4409411/1‑140 (MQ=255)
                                                             gTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGcc                                                                                                                            >  2:4859972/1‑92 (MQ=255)
                                                             gTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACtt                                                                            >  1:1523672/1‑140 (MQ=255)
                                                             gTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACtt                                                                            >  1:946034/1‑140 (MQ=255)
                                                             gTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACtt                                                                            >  1:2713127/1‑140 (MQ=255)
                                                               aaTGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTa                                                                          <  1:825646/140‑1 (MQ=255)
                                                               aaTGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTa                                                                          <  1:613301/140‑1 (MQ=255)
                                                                 tGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACg                                                                        <  1:3119397/140‑1 (MQ=255)
                                                                      aTTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGAt                                                                                                                                        >  1:2325115/1‑71 (MQ=255)
                                                                      aTTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAATTGATTTTATTTTTATTTATAAATCAGAAAGGCCTTTACGataat                                                                   >  2:2931643/1‑140 (MQ=255)
                                                                      aTTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGataat                                                                   >  1:3772560/1‑140 (MQ=255)
                                                                        ttGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGAtaataa                                                                 >  1:2737693/1‑140 (MQ=255)
                                                                        ttGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATAAGAAAGGACTTTAGGAtaataa                                                                 >  1:2906179/1‑140 (MQ=255)
                                                                        ttGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATAAGAAAGGACTTTACGAtaataa                                                                 >  2:1195336/1‑140 (MQ=255)
                                                                         tGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAg                                                                <  1:3299880/140‑1 (MQ=255)
                                                                             tCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATAAGAAAGGACTTTACTATAATAATCAAg                                                            >  2:4585266/1‑140 (MQ=255)
                                                                                     gtGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACGTTACGATAATAAGCAAGAATTCttt                                                    >  2:874486/1‑140 (MQ=255)
                                                                                         aaTGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTa                                                <  2:3379701/140‑1 (MQ=255)
                                                                                         aaTGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTa                                                <  2:3687381/140‑1 (MQ=255)
                                                                                         aaTGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTa                                                <  1:1005377/140‑1 (MQ=255)
                                                                                          aTGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTaa                                               >  1:1747470/1‑140 (MQ=255)
                                                                                             cACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGa                                            >  1:112613/1‑140 (MQ=255)
                                                                                              aCGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGat                                           <  1:4527039/140‑1 (MQ=255)
                                                                                              aCGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGat                                           <  1:2181611/140‑1 (MQ=255)
                                                                                              aCGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGat                                           <  1:1725195/140‑1 (MQ=255)
                                                                                              aCGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGat                                           <  1:242411/140‑1 (MQ=255)
                                                                                                gTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAATCAAGAATGCTTTTTTAATGatca                                         >  1:688417/1‑140 (MQ=255)
                                                                                                   caaTTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGAtcatca                                      <  2:3882970/138‑1 (MQ=255)
                                                                                                     aTTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGa                                    <  1:1986827/140‑1 (MQ=255)
                                                                                                     aTTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGa                                    >  2:4984126/1‑140 (MQ=255)
                                                                                                       ttttGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGaaa                                  <  2:1392756/140‑1 (MQ=255)
                                                                                                       ttttGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGaaa                                  <  2:2322409/140‑1 (MQ=255)
                                                                                                         ttGGATGATATTTCATGATTTTCACACCTTTTTTATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATCAGCAAGAATGCTTTTTTAATGATCATCAGAAagg                                <  1:5122760/140‑1 (MQ=255)
                                                                                                         ttGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAagg                                <  2:177922/140‑1 (MQ=255)
                                                                                                            gatgatATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGGACGAATGCTTTTTTAATGATCATCAGACaggagg                             >  2:3957762/1‑140 (MQ=255)
                                                                                                                     tCATGATTTTCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGa                    <  1:2696296/140‑1 (MQ=255)
                                                                                                                     tCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGa                    <  1:987570/140‑1 (MQ=255)
                                                                                                                     tCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGa                    <  2:1519260/140‑1 (MQ=255)
                                                                                                                     tCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGa                    <  1:4326539/140‑1 (MQ=255)
                                                                                                                      cATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATACACAAATCATTTTATTATTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATTAg                   <  1:3539691/140‑1 (MQ=255)
                                                                                                                       aTGATTTGCACAGCTTTTTGATTGTATTTATGCCTATTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGc                  <  2:221873/140‑1 (MQ=255)
                                                                                                                       aTGATTTGCACACCTTTTTGATTTTTTTTATGCCTATTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGc                  <  1:2076710/140‑1 (MQ=255)
                                                                                                                       aTGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGc                  <  2:856609/140‑1 (MQ=255)
                                                                                                                       aTGATTTGCAAACCTTTTTGATTGTATTTATGCCTATTATACACAAATCATTTTATTTTTATTTAAAAATAAGAAAGGACTTTAGGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGc                  <  1:4224172/140‑1 (MQ=255)
                                                                                                                            ttccacaCCTTTTTGATTTTATTTATTCCTAGTATAAACAAATCATTTTAATTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGaa             <  1:3111950/137‑1 (MQ=255)
                                                                                                                            ttGCACACGTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGaa             <  2:1619556/140‑1 (MQ=255)
                                                                                                                            ttGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGaa             <  2:3113745/140‑1 (MQ=255)
                                                                                                                                  acatttttGATTTTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGAAATACtt       <  2:1846923/137‑1 (MQ=255)
                                                                                                                                  acCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGAAATACtt       <  2:3772560/140‑1 (MQ=255)
                                                                                                                                    cTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGAAATACTTTg     >  1:2560566/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
GACCAAATTCAGATGCTGTTTCAGTAAGATAACCGGTCGTTTCGGGATCTGCGTGATCGCTGTAATGCAGATTTGCTTCACCGTCGTGAAATGCACGTCCCATTTTTGGATGATATTTCATGATTTGCACACCTTTTTGATTGTATTTATGCCTAGTATAAACAAATCATTTTATTTTTATTTAAAAATCAGAAAGGACTTTACGATAATAAGCAAGAATGCTTTTTTAATGATCATCAGAAAGGAGGACACAATGAGCAAGAAATACTTTGTTC  >  NZ_CP034943/2627059‑2627333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: