Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,632,871 A→G 5.4% M575T (ATG→ACG)  recJ ← single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,632,8710AG 5.4% 740.9 / 21.2 221M575T (ATG→ACG) recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base A (113/95);  new base G (6/6);  total (120/101)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 235 total aligned reads displayed.

CGCCTTCGCTTGATCTTCAGAGCTGATGACGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAACCCGGGACAATGC  >  NZ_CP034943/2632732‑2633009
                                                                                                                                           |                                                                                                                                          
    ttCGCTTGATCTTCAGAGCTGATTACGTGCACTTCACGGCGAAGGTCTTCAGTCTGAATAAGTGTTGTTGAGTCTTCTTTGAAATAGACAATGGCCCGTTTAGAATATGGAACGACGGAAACTGTGTCTTCCCACATcag                                                                                                                                        >  2:4586465/1‑138 (MQ=255)
    ttCGCTTGATCTTCAGAGCTGATGACGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCg                                                                                                                                        >  1:3996682/1‑140 (MQ=255)
    ttCGCTTGATCTTCAGAGCTGATGACGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCg                                                                                                                                        >  2:1477857/1‑140 (MQ=255)
            aTCTTCAGAGCTGATGACGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCcaca                                                                                                                                            <  2:3730790/128‑1 (MQ=255)
               ttCAGAGCTGATGACGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTa                                                                                                                             <  1:192000/140‑1 (MQ=255)
                         atgaCGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAg                                                                                                                   >  2:1574094/1‑140 (MQ=255)
                             cGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGc                                                                                                               <  2:6001/140‑1 (MQ=255)
                             cGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGc                                                                                                               <  2:530387/140‑1 (MQ=255)
                             cGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGc                                                                                                               <  2:4930958/140‑1 (MQ=255)
                              gTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGcc                                                                                                              >  2:2537116/1‑140 (MQ=255)
                              gTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGcc                                                                                                              >  1:5063142/1‑140 (MQ=255)
                                gCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCAt                                                                                                            >  2:840214/1‑140 (MQ=255)
                                 cACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCAtt                                                                                                           <  2:5167393/140‑1 (MQ=255)
                                      cACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCCTTCAGaa                                                                                                      >  1:3200623/1‑140 (MQ=255)
                                      cACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGaa                                                                                                      >  1:2592757/1‑140 (MQ=255)
                                      cACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGaa                                                                                                      >  1:4616908/1‑140 (MQ=255)
                                      cACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGaa                                                                                                      >  2:2144970/1‑140 (MQ=255)
                                      cACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTCAAATCAAACAGCTGCCATTCAGaa                                                                                                      <  2:337731/140‑1 (MQ=255)
                                      cACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTATCCCCTTAAATCAAACAGCTGCCATTCAGaa                                                                                                      >  1:545802/1‑140 (MQ=255)
                                       aCGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGaaa                                                                                                     <  1:4460118/140‑1 (MQ=255)
                                        cGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAAc                                                                                                    >  2:3106026/1‑140 (MQ=255)
                                        cGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAAc                                                                                                    >  2:3521846/1‑140 (MQ=255)
                                             aaGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGTACGGACGGAAACTTTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGAGATTCAGAAACAGCgg                                                                                               <  1:1880390/140‑1 (MQ=255)
                                             aaGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCgg                                                                                               <  1:3140667/140‑1 (MQ=255)
                                             aaGGTATTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCgg                                                                                               <  1:3879212/140‑1 (MQ=255)
                                              aGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGc                                                                                              >  1:439868/1‑140 (MQ=255)
                                              aGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGc                                                                                              <  2:4082099/140‑1 (MQ=255)
                                              aGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGc                                                                                              >  1:2680281/1‑140 (MQ=255)
                                                gTCTTCAGTTTGAAGTAATGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGGCGGAAACTGTGTCTTCCCACGTCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCAt                                                                                            >  1:878189/1‑140 (MQ=255)
                                                gTCTTCAGTTTGAAGTAATGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGGCGGAAACTGTGTCTTCCCACGTCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCAt                                                                                            >  1:1281046/1‑140 (MQ=255)
                                                gTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCAt                                                                                            <  1:4177693/140‑1 (MQ=255)
                                                   ttCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCt                                                                                         <  2:3218338/140‑1 (MQ=255)
                                                   ttCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCt                                                                                         <  2:293362/140‑1 (MQ=255)
                                                     cAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTa                                                                                       >  2:3593730/1‑140 (MQ=255)
                                                     cAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTa                                                                                       >  1:3307247/1‑140 (MQ=255)
                                                          tGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCAt                                                                                  >  1:913857/1‑140 (MQ=255)
                                                          tGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCAt                                                                                  >  2:1229446/1‑140 (MQ=255)
                                                            aagaagTGTTGTTGAGACTTCTTAGAAAGAGACAATGGCCAGTTTAGCAGATGGAAGGACGGAAACAGAGTCTTCCCACAACCGTTATCCCCTTAAATCAAACAGCAGCCATTCAGAAACAGCGGCATAATTAATCaaaa                                                                                >  2:1297924/1‑137 (MQ=255)
                                                                  tgttgtTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGCCGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCGTCATCATTCGCTGCg                                                                          >  1:491858/1‑140 (MQ=255)
                                                                  tgttgtTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTgag                                                                          >  1:2849537/1‑138 (MQ=255)
                                                                  tgttgtTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCg                                                                          >  1:1043016/1‑140 (MQ=255)
                                                                  tgttgtTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTGAATCATTAGCTGCg                                                                          >  2:3904601/1‑140 (MQ=255)
                                                                    ttgttgAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGt                                                                        >  1:1571573/1‑140 (MQ=255)
                                                                      gttgAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttt                                                                      >  1:367519/1‑140 (MQ=255)
                                                                      gttgAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttt                                                                      >  2:3415634/1‑140 (MQ=255)
                                                                      gttgAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttt                                                                      >  2:1927402/1‑140 (MQ=255)
                                                                        tgtgTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttttt                                                                    <  1:4508340/137‑1 (MQ=255)
                                                                        tgAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttttt                                                                    <  1:4197966/140‑1 (MQ=255)
                                                                        tgAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttttt                                                                    <  1:3830996/140‑1 (MQ=255)
                                                                        tgAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttttt                                                                    <  1:4755278/140‑1 (MQ=255)
                                                                        tgAGTCTTCTTTGAAAGAGACAATGGCCCCTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGttttt                                                                    <  1:5074689/140‑1 (MQ=255)
                                                                            tcttctTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCg                                                                                                <  2:4681942/108‑1 (MQ=255)
                                                                             cttcttTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTa                                                               <  2:882726/140‑1 (MQ=255)
                                                                             cttcttTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTa                                                               <  1:681530/140‑1 (MQ=255)
                                                                              ttcttTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTAt                                                              >  1:2002013/1‑140 (MQ=255)
                                                                                     aaaGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCa                                                       <  1:1153872/140‑1 (MQ=255)
                                                                                     aaaGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCa                                                       <  2:3704711/140‑1 (MQ=255)
                                                                                      aaGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCAt                                                      <  1:3593730/140‑1 (MQ=255)
                                                                                         agaCAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCg                                                   >  2:2735436/1‑140 (MQ=255)
                                                                                          gaCAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGt                                                  <  2:3309468/140‑1 (MQ=255)
                                                                                          gaCAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGt                                                  <  2:2260500/140‑1 (MQ=255)
                                                                                           aCAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGc                                                                                              >  1:3370390/1‑95 (MQ=255)
                                                                                             aaTGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGa                                               >  2:712807/1‑140 (MQ=255)
                                                                                              aTGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGAt                                              <  1:3471821/140‑1 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGACACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCCATTGTTCCATTCGTTGaga                                             >  1:264290/1‑138 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACCTCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCAGCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATa                                             >  1:3547245/1‑140 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAACTCACACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTGTTTTTCTAGTGTTCCATTCGTTGAtg                                             >  1:306570/1‑139 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATa                                             <  2:2691615/140‑1 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATa                                             <  2:4830558/140‑1 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATa                                             >  1:275561/1‑140 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATa                                             >  1:429776/1‑140 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATa                                             >  2:4369578/1‑140 (MQ=255)
                                                                                               tGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATa                                             >  1:3432811/1‑140 (MQ=255)
                                                                                                    cGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCAt                                                      >  1:175151/1‑126 (MQ=255)
                                                                                                    cGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCAt                                                      <  2:175151/126‑1 (MQ=255)
                                                                                                     gTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACAACCGTTTTCCCATTAAATCAAACAGCTCCCATTCAGACACAGCGGCATCCATAATCATTAGATGCGGTTTTTTTCTATTGTTCCAATCGAGGATATACAta                                       >  2:3914638/1‑139 (MQ=255)
                                                                                                              aTGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCgg                                                                                               >  2:2745192/1‑75 (MQ=255)
                                                                                                                ggAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAAt                            >  2:2457543/1‑140 (MQ=255)
                                                                                                                ggAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAAt                            >  1:621805/1‑140 (MQ=255)
                                                                                                                ggAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTACATTCGTTGATAGACATCTCACCGACAAt                            >  1:4823116/1‑140 (MQ=255)
                                                                                                                   aGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGa                         <  2:4780304/140‑1 (MQ=255)
                                                                                                                   aGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTAAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGa                         <  2:1355722/140‑1 (MQ=255)
                                                                                                                        ggAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTc                    >  1:1795778/1‑140 (MQ=255)
                                                                                                                        ggAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTc                    >  2:1832019/1‑140 (MQ=255)
                                                                                                                          aaaCTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCtt                  <  2:1889542/140‑1 (MQ=255)
                                                                                                                          aaaCTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCtt                  <  2:1644867/140‑1 (MQ=255)
                                                                                                                               gtgtCTTCCCACGTCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTTATCATAAGCTGCGGCTTTTTTCGATTGTTCCATTCGTTGATTGACATCTCACCGACAATGGAAATTCTTGAAcc             <  2:2639222/140‑1 (MQ=255)
                                                                                                                               gtgtCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGTTAGACATCTCACCGACAATTGAAATTCTTGAAcc             >  1:1422208/1‑140 (MQ=255)
                                                                                                                               gtgtCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAAcc             <  1:2735633/140‑1 (MQ=255)
                                                                                                                               gtgtCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAAcc             <  1:840214/140‑1 (MQ=255)
                                                                                                                               gtgtCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAAcc             >  1:3320865/1‑140 (MQ=255)
                                                                                                                               gtgtCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATATCACCGACAATTGAAATTCTTTAAcc             >  1:4088782/1‑140 (MQ=255)
                                                                                                                                tgtCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAAccc            >  2:3129477/1‑140 (MQ=255)
                                                                                                                                      cccACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAACCCGGGACa      <  2:1043016/140‑1 (MQ=255)
                                                                                                                                        cacaTCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAAt                            <  2:4502923/116‑1 (MQ=255)
                                                                                                                                          caTCCGTTTTCCCCTTAAATCACACAGCTGCCATTCAGACACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATATCACCGACAATTGAAATTCTTGAACCCGGGACAATGc  >  2:3182090/1‑140 (MQ=255)
                                                                                                                                          caTCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGaa                        <  1:2348708/118‑1 (MQ=255)
                                                                                                                                          caTCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGaa                        >  2:2348708/1‑118 (MQ=255)
                                                                                                                                          caTCCGTTTTCCCCTTAAATCAAAAAGCTGCCATTCAGAAACAGCGGCATCATTAATAATTAGCTGCGGTTTTTTTCTATTGTTACAATCGTTGATAGACATCTCACCGACAAATCAAATTCTTGAAACCGCGACACTGc  >  2:5107545/1‑140 (MQ=255)
                                                                                                                                          caTCCGTGTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAACCCGGGACAATGc  >  1:1634013/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CGCCTTCGCTTGATCTTCAGAGCTGATGACGTGCACTTCACGGCGAAGGTCTTCAGTCTGAAGAAGTGTTGTTGAGTCTTCTTTGAAAGAGACAATGGCCCGTTTAGCAGATGGAAGGACGGAAACTGTGTCTTCCCACATCCGTTTTCCCCTTAAATCAAACAGCTGCCATTCAGAAACAGCGGCATCCTTAATCATTAGCTGCGGTTTTTTTCTATTGTTCCATTCGTTGATAGACATCTCACCGACAATTGAAATTCTTGAACCCGGGACAATGC  >  NZ_CP034943/2632732‑2633009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: